In 2022, an unprecedented outbreak of mpox raged in several nations. Sequences from the 2022 outbreak reveal a higher nucleotide substitution if compared with the estimated rate for orthopoxviruses. Recently, intra-lesion SNVs (single nucleotide variants) have been described, and these have been suggested as possible sources of genetic variation. Until now, it has not been clear if the presence of several SNVs could represents the result of local mutagenesis or a possible co-infection. We investigated the significance of SNVs through whole-genome sequencing analysis of four unrelated mpox cases. In addition to the known mutations harboured by the circulating strains of virus (MPXV), 7 novel mutations were identified, including SNVs located in genes that are involved in immune evasion mechanisms and/or viral fitness, six of these appeared to be APOBEC3-driven. Interestingly, three patients exhibited the coexistence of mutated and wild-type alleles for five non-synonymous variants. In addition, two patients, apparently unrelated, showed an analogous pattern for two novel mutations, albeit with divergent frequencies. The coexistence of mixed viral populations, harbouring non-synonymous mutations in patients, supports the hypothesis of possible co-infection. Additional investigations of larger clinical cohorts are essential to validating intra-patient viral genome heterogeneity and determining the possibility of co-presence events of slightly divergent MPXV strains. Full article

mTOR is a central regulator of cell growth and metabolism in response to mitogenic and nutrient signals. Notably, mTOR is not only found in the cytoplasm but also in the nucleus. This review highlights direct involvement of nuclear mTOR in regulating transcription factors, orchestrating epigenetic modifications, and facilitating chromatin remodeling. These effects intricately modulate gene expression programs associated with growth and metabolic processes. Furthermore, the review underscores the importance of nuclear mTOR in mediating the interplay between metabolism and epigenetic modifications. By integrating its functions in nutrient signaling and gene expression related to growth and metabolism, nuclear mTOR emerges as a central hub governing cellular homeostasis, malignant transformation, and cancer progression. Better understanding of nuclear mTOR signaling has the potential to lead to novel therapies against cancer and other growth-related diseases. Full article

The Chinese Loess Plateau has undergone extensive revegetation to restore degraded land and enhance carbon sequestration. However, soil organic carbon (SOC) sequestrated in the soil profiles of deep-rooted plants has not been fully studied. Here, we investigated the SOC within a 0–23 m profile in farmlands and apple orchards converted from farmlands with different ages (A5, <5 years; A10, ~10 years; A15, ~15 years; A20, >20 years) and the controlling factors on three loess tablelands (Changwu, Qingyang, and Luochuan). The results show that SOC stocks among farmlands and orchards showed no significant difference (p = 0.88); however, SOC stocks showed a trend with tree ages, i.e., a decrease for A5 and A10 but an increase for A15 and A20. For the vertical variability, the SOC stock was the highest within 0–1 m, regardless of the standing age; however, the SOC stock in this layer only accounted for 8.8% of the total SOC stock (97.93 ± 9.18 kg m⁻²). Climate accounted for 82% of the variations and controlled the changes in SOC in the 0–1 m range, while soil texture dominated the SOC in the soil below 1 m, accounting for 57% of the SOC variations. The variations in SOC in the thick, unsaturated zones provide implications for future land use management and the sustainability of apple orchards in arid regions. Full article

In this study, the fatigue strength of polished drill pipe specimens was investigated and compared with previous test results of corroded and not-corroded pipes. The resonant fatigue test rig, which was designed and implemented by the University of Pisa, is initially presented by providing a detailed description of the set-up of the machine, the calibration of the strain gauges, the control system, and the correct identification of the vibrational node locations. A polishing rig was also designed and put into operation to remove the corrosion pits from the outer surface of almost the entire length of the drill pipe specimens. After the fatigue tests with the resonant rig, and the observation of the fatigue fracture of the specimens, a few samples were extracted from different zones (corroded and not corroded) of the failed drill pipe specimens. This allowed for investigations to be carried out using a scanning electronic microscope. The obtained results were analyzed using the Murakami model, and a discussion is presented about the effect of the corrosion pits on the fatigue strength. Full article

Many liqueurs, including spirits infused with botanicals, are crafted not only for their taste and flavor but also for potential medicinal benefits. However, the scientific evidence supporting their medicinal effects remains limited. This study aims to verify in vitro anticancer activity and bioactive compounds in shochu spirits infused with Cordyceps militaris, a Chinese medicine. The results revealed that a bioactive fraction was eluted from the spirit extract with 40% ethanol. The infusion time impacted the inhibitory effect of the spirit extract on the proliferation of colon cancer-derived cell line HCT-116 cells, and a 21-day infusion showed the strongest inhibitory effect. Furthermore, the spirit extract was separated into four fractions, A-D, by high-performance liquid chromatography (HPLC), and Fractions B, C, and D, but not A, exerted the effects of proliferation inhibition and apoptotic induction of HCT-116 cells and HL-60 cells. Furthermore, Fractions B, C, and D were, respectively, identified as adenosine, cordycepin, and N⁶-(2-hydroxyethyl)-adenosine (HEA) by comprehensive chemical analyses, including proton nuclear magnetic resonance (¹H-NMR), Fourier transform infrared spectroscopy (FT-IR), and electrospray ionization mass spectrometry (ESI-MS). To better understand the bioactivity mechanisms of cordycepin and HEA, the agonist and antagonist tests of the A3 adenosine receptor (A3AR) were performed. Cell viability was suppressed by cordycepin, and HEA was restored by the A3AR antagonist MR1523, suggesting that cordycepin and HEA possibly acted as agonists to activate A3ARs to inhibit cell proliferation. Molecular docking simulations revealed that both adenosine and cordycepin bound to the same pocket site of A3ARs, while HEA exhibited a different binding pattern, supporting a possible explanation for the difference in their bioactivity. Taken together, the present study demonstrated that cordycepin and HEA were major bioactive ingredients in Cordyceps militaries-infused sweet potato shochu spirits, which contributed to the in vitro anticancer activity. Full article

African horse sickness is a severe and often fatal disease affecting all species of equids. The aetiological agent, African horse sickness virus (AHSV), can be differentiated into nine serotypes. The identification of AHSV serotypes is vital for disease management, as this can influence vaccine selection and help trace disease incursion routes. In this study, we report the development and optimisation of a novel, molecular-based assay that utilises multiplex PCR and microsphere-based technology to expedite detection and differentiation of multiple AHSV serotypes in one assay. We demonstrated the ability of this assay to identify all nine AHSV serotypes, with detection limits ranging from 1 to 277 genome copies/µL depending on the AHSV serotype. An evaluation of diagnostic sensitivity and specificity revealed a sensitivity of 88% and specificity of 100%. This method can serotype up to 42 samples per run and can be completed in approximately 4–6 h. It provides a powerful tool to enhance the rapidity and efficiency of AHSV serotype detection, thereby facilitating the generation of epidemiological data that can help understand and control the incidence of AHSV worldwide. Full article

Preclinical evidence indicates the potential anti-tumor capabilities of cannabinoids in prostate cancer (PC). We undertook a cross-sectional study using National Survey on Drug Use and Health data from 2002 to 2020, involving 2503 participants in the USA. The independent variable was marijuana use status (current, former, never), while the dependent variable was self-reported PC (yes, no). Eleven other demographic variables were assessed as covariates. PC prevalence was lower among current marijuana users (46/145, 31.7%) and former users (323/1021, 31.6%) compared to non-users (534/1337, 39.9%, p < 0.001). PC prevalence was lower among users versus non-users in the elderly (≥65) (36.4% vs. 42.4%, p = 0.016) and non-Hispanic white subgroups (28.9% vs. 38.3%, p < 0.001). There were no significant PC prevalence differences between users and non-users in the younger population (50–64) or other race/ethnicity. In the multivariable analyses, former marijuana use was associated with lower PC compared to never using (odd ratio = 0.74, 95% CI 0.62–0.90, p = 0.001). Current use was also suggestive of reduced prevalence but was not statistically significant (odd ratio = 0.77, 95% CI 0.52–1.14, p = 0.198), possibly due to low sample size. Our findings from a large national survey provide additional data to link marijuana use with lower PC prevalence. Full article

Ultrasonic vibration-assisted con-rod fracture splitting (UV-CFS) was used to carry out the fracture experiment of 1045 quenched and tempered steel. The effect of ultrasonic vibration on the fracture properties was studied, the fracture microstructure and the evolution of dislocations near the fracture were analyzed and the microscopic mechanism was analyzed. The results show that in the case of conventional fracture splitting without amplitude, the dimple and the fracture belong to ductile fracture. With the increase in ultrasonic amplitude, the plasticity and pore deformation of the con-rod samples decrease at first and then increase; when the amplitude reaches a certain point, the load required for cracking is reduced to a minimum and the ultrasonic hardening effect is dominant, resulting in a decrease in the plasticity of the sample, a cleavage fracture, a brittle fracture, the minimum pore deformation and high cracking quality. The research results also show that with the increase in ultrasonic amplitude, the fracture dislocation density decreases at first, then increases, and dislocation entanglement and grain breakage appear, then decrease, and multiple dislocation slip trajectories appear. The changes in the dislocation density and microstructure are consistent with the above results. Full article

An accurate simulation and projection of future warming are needed for a proper policy response to expected climate change. We examine the simulations of the mean global and Arctic surface air temperatures by the CMIP6 (Climate Models Intercomparison Project phase 6) climate models. Most models overestimate the observed mean global warming. Only seven out of 19 models considered simulate global warming that is within ±15% of the observed warming between the average of the 2014–2023 and 1961–1990 reference period. Ten models overestimate global warming by more than 15% and only one of the models underestimates it by more than 15%. Arctic warming is simulated by the CMIP6 climate models much better than the mean global warming. The reason is an equal spread of over and underestimates of Arctic warming by the models, while most of the models overestimate the mean global warming. Eight models are within ±15% of the observed Arctic warming. Only three models are accurate within ±15% for both mean global and Arctic temperature simulations. Full article

RAMOSA1 (RA1) is a Cys2-His2-type (C2H2) zinc finger transcription factor that controls plant meristem fate and identity and has played an important role in maize domestication. Despite its importance, the origin of RA1 is unknown, and the evolution in plants is only partially understood. In this paper, we present a well-resolved phylogeny based on 73 amino acid sequences from 48 embryophyte species. The recovered tree topology indicates that, during grass evolution, RA1 arose from two consecutive SUPERMAN duplications, resulting in three distinct grass sequence lineages: RA1-like A, RA1-like B, and RA1; however, most of these copies have unknown functions. Our findings indicate that RA1 and RA1-like play roles in the nucleus despite lacking a traditional nuclear localization signal. Here, we report that copies diversified their coding region and, with it, their protein structure, suggesting different patterns of DNA binding and protein–protein interaction. In addition, each of the retained copies diversified regulatory elements along their promoter regions, indicating differences in their upstream regulation. Taken together, the evidence indicates that the RA1 and RA1-like gene families in grasses underwent subfunctionalization and neofunctionalization enabled by gene duplication. Full article

In contextual vocabulary acquisition, second language learners address both morphological and contextual cues to infer the meanings of novel words. The study investigated cognitive strategies and their eye movement correlates in contextual vocabulary inferencing task. A sample of 30 healthy participants with normal or corrected to normal vision read an Intermediate-level text in English, aiming to define the meaning of 10 low-frequency words embedded in the text. Based on both unstructured and structured post-hoc reports, we attributed the inferencing process either to word-level processing, context-level processing, or discourse-level processing strategy. Eye movements were recorded on the target word and on the target word clause areas of interest. Inferencing success was associated with increased first fixation duration on the target word both for word- and context-level processing strategy, and increased dwell time on the target word clause for context-level processing strategy. Our findings emphasize the role of both morphological and contextual processing in vocabulary inferencing task. Full article

High-precision tapered threads are widely used in hard-loaded mechanical joints, especially in the aggressive environment of the drilling of oil and gas wells. Therefore, they must be made of workable materials often difficult to machine. This requires the use of high-performance cutting tools, which means the application of non-zero geometric parameters: rake and edge inclination angles. This study is based on analytical geometry methodology and describes the theoretical function of the thread profile as convoluted surfaces dependent on the tool’s geometric angles. The experiments were conducted using a visual algorithm grounded on the obtained function and prove the practical use of the scientific result. They predict the required accuracy of thread made using a lathe tool with a rake angle of up to 12°. Full article

In this paper, a six-degree-of-freedom analog tactile probe with a new, simple, and robust mechanical design is presented. Its design is based on the use of one elastomeric ring that supports the stylus carrier and allows its movement inside a cubic measuring range of ±3 mm. The position of the probe tip is determined by three low-cost, noncontact, 2D PSD (position-sensitive detector) sensors, facilitating a wider application of this probe to different measuring systems compared to commercial ones. However, several software corrections, regarding the size and orientation of the three LED light beams, must be carried out when using these 2D sensors for this application due to the lack of additional focusing or collimating lenses and the very wide measuring range. The development process, simulation results, correction models, experimental tests, and calibration of this probe are presented. The results demonstrate high repeatability along the X-, Y-, and Z-axes (2.0 µm, 2.0 µm, and 2.1 µm, respectively) and overall accuracies of 6.7 µm, 7.0 µm, and 8.0 µm, respectively, which could be minimized by more complex correction models. Full article

Using the solid-state reactions method Na_0.55(Co,M)O₂ (M = Cr, Ni, Zn, W, and Bi) ceramics were prepared and their crystal structure, microstructure, electrophysical, thermophysical, and thermoelectric properties were studied. Doping of Na_0.55CoO₂ by transition or heavy metal oxides led to the increase in the grain size of ceramics, a decrease in electrical resistivity and thermal diffusivity values, and a sharp increase in the Seebeck coefficient, which resulted in essential enhancement of their thermoelectric properties. The largest power factor (1.04 mW/(m·K²) at 1073 K) and figure of merit (0.702 at 1073 K) among the studied samples possessed the Na_0.55Co_0.9Bi_0.1O₂ compound, which also demonstrated the highest values of the Seebeck coefficient (666 μV/K at 1073 K). The obtained results show that the doping of layered sodium cobaltite by different metal oxides allows for improving its stability, microstructure, and functional properties, which proves the effectiveness of the doping strategy for developing new thermoelectric oxides with enhanced thermoelectric performance. Full article

The Anthropocene, marked by human-induced climate change, necessitates urgent action to address climate goals and respect planetary boundaries. While sustainability research provides knowledge, the first challenge lies in communicating the findings in an adequate manner to the public and several stakeholders, such as economic and political actors. Therefore, this study explores the significance of science communication in sustainability science, focusing on a case study—the True Cost Accounting (TCA) campaign by the University of Greifswald, Technical Institute of Nuremberg, and German retailer PENNY. TCA herein serves as a transparency tool, economic incentive, and discussion basis for sustainable consumption. This study investigates consumer perceptions of ecological prices of foods through a face-to-face survey during the 2023 PENNY campaign, comparing results to an informational campaign carried out in 2021. Findings indicate a high awareness of the true cost campaign in 2023, with 50.8% of participants hearing about it. Consumers’ willingness to pay true costs and potential behavior changes were explored. In comparison to results from the informational campaign of 2021, customers showed a decrease in this WTP when the true prices would actually impact their spending, indicating an attitude–behavior gap. In addition, a willingness to reduce the consumption of animal foods—if TCA was implemented—of 60.5% was determined, which suggests that TCA has the potential for sustainable behavior change. This study highlights factors that influence consumer attitudes and preferences regarding the inclusion of TCAs, such as environmental, social, and animal welfare costs. Customers’ understanding of increased prices—like, in this case, the compensation for environmental and social costs—is an argument in favor of true prices. The results emphasize the need for differentiated scientific communication strategies to bridge knowledge and action gaps in sustainability science. Full article

Internet-of-things networks consist of multiple sensor devices spread over a wide area. In order to protect the data from unauthorized access and tampering, it is essential to ensure secure communication between the sensor devices and the central server. This security measure aims to guarantee authenticity, confidentiality, and data integrity. Unlike traditional computing systems, sensor node devices are often limited regarding memory and computing power. Lightweight communication protocols, such as LoRaWAN, were introduced to overcome these limitations. However, despite the lightweight feature, the protocol is vulnerable to different types of attacks. This proposal presents a highly secure key establishment protocol that combines two cryptography schemes: Elliptic Curve Qu–Vanstone and signcryption key encapsulation. The protocol provides a method to establish a secure channel that inherits the security properties of the two schemes. Also, it allows for fast rekeying with only one exchange message, significantly reducing the handshake complexity in low-bandwidth communication. In addition, the selected schemes complement each other and share the same mathematical operations in elliptic curve cryptography. Moreover, with the rise of a community-friendly platform like RISC-V, we implemented the protocol on a RISC-V system to evaluate its overheads regarding the cycle count and execution time. Full article

In this study, TiO₂ nanospheres (TiO₂-NS) were synthesized by the solvothermal method. Firstly, the synthesized nanomaterial was characterized by X-ray diffraction (XRD), Fourier Transformed Infrared (FTIR), scanning electron microscopy (SEM) and UV-Vis Diffuse Reflectance Spectroscopy (DRS). To study the photocatalytic degradation of Tartrazine (TTZ) and Naphthol Blue Black (NBB) in a binary mixture, the influence of some key parameters such as pH, pollutant concentration and catalyst dose was taken into account under visible and UV light. The results show a 100% degradation efficiency for TTZ after 150 min of UV irradiation and 57% under visible irradiation at 180 min. The kinetic study showed a good pseudo-first-order fit to the Langmuir–Hinshelwood model. Furthermore, in order to get closer to the real conditions of textile wastewater, the influence of the presence of salt on TiO₂-NS’s photocatalytic performance was explored by employing NaCl as an inorganic ion. The optimum conditions provided by the Response Surface Methodology (RSM) were low concentrations of TTZ (2 ppm) and NBB (2.33 ppm) and negligible salt (NaCl) interference. The percentage of photodegradation was high at low pollutant and NaCl concentrations. However, this yield became very low as NaCl concentrations increased. The photocatalytic treatment leads to 31% and 53% of mineralization yield after 1 and 3 h of visible light irradiation. The synthesis of TiO₂-NS provides new insights that will help to develop an efficient photocatalysts for the remediation of contaminated water. Full article

This article presents an analysis of the performance of a 14.04 kWp grid-connected photovoltaic (PV) installation consisting of monocrystalline silicon, polycrystalline silicon and bifacial glass–glass monocrystalline silicon modules. The photovoltaic system was mounted in Poland, a location characterized by temperate climate conditions. On the basis of the obtained results, the photovoltaic parameters were determined in accordance with the international standard. The annual energy yield of the entire system was 1033 kWh/kWp, and the performance ratio achieved was 83%. The highest average daily final yield was in the range of 4.0–4.5 kWh/kWp for each photovoltaic technology under investigation. In the cold part of the year, the efficiency of the photovoltaic modules was estimated to be 15%, and it was estimated to be 7% during the warm part of the year. Array capture losses accounted for around 0.75 kWh/kWp of energy loss per day, whereas the inverter efficiency was over 95% during the months that are beneficial for energy production. Full article

In 2021, the American Association of Colleges of Nursing (AACN) added “the impact of climate change on environmental and population health” into The Essentials: Core Competencies for Professional Nursing Education. Presently, little guidance exists for nursing faculty new to climate education. The year prior, the Nurses Climate Challenge (NCC)—a campaign to educate 50,000 health professionals about health impacts of climate change—launched the School of Nursing Commitment through a series of focus groups and collaborative content development. With an aim of increasing access to knowledge and tools to support education about the health impacts of climate change, the NCC Commitment partners with nursing schools and provides a community of practice. Partner schools use NCC resources in courses and report the number of students educated. Within three years, 61 nursing schools in 30 states joined the Commitment. Participants included academic health centers, research institutions, multi-state schools, and small private colleges, and programs ranged from AD to PhD. Faculty (1) integrated resources into didactic and clinical settings, such as population or organ-system content, leadership, and policy; and (2) used resources to support assignments. In four years, faculty reported educating over 37,700 students, using NCC resources in 439 educational sessions. The Commitment may be valuable for faculty fulfilling AACN Essentials by bringing climate change to the classroom, community, and bedside. Furthermore, the Commitment may be a replicable model for health professional education and inspiring action on climate change. Full article

A PLS-based quality prediction method is proposed for batch processes using two-dimensional extended windows. To realize the adoption of information in the directions of sampling time and batch, a newly defined region of support (ROS), called the k-i-back-extended region of support (KIBROS), is proposed; it establishes an extended window by adding two regions of data to the traditional ROS to include all possible important data for quality prediction. Based on the new ROS, extended windows are established, and different models are proposed using the extended windows for batch process quality prediction. Furthermore, using the typical injection molding batch process as an example, the proposed quality prediction method is experimentally verified, proving that the proposed methods have higher prediction accuracy than the traditional method and that the prediction stability is also improved. Full article

Mitochondrial genomes are playing an increasingly important role in molluscan taxonomy, germplasm, and evolution studies. The first complete mitochondrial genome of the commercial big brown mactra clam, Mactra grandis, was characterized using Illumina next-generation sequencing in this study. The 17,289 bp circular genome has a typical gene organization of 13 protein-coding genes (PCGs), 2 rRNAs, and 22 tRNAs, with an obvious (A + T)-bias of 64.54%. All PCGs exhibited a homogeneous bias in nucleotide composition with a (A + T)-bias, a positive GC skew, and a negative AT skew. Results of phylogenetic analysis showed that Mactra grandis was most closely related to Mactra cygnus. The functional gene arrangement of the two species was identical but different from other Mactra species. The congeneric relationships among Mactra species were demonstrated by genetic distance analysis. Additionally, the selective pressure analysis suggested that cox1 was highly efficient for discriminating closely related species in genus Mactra, while nad2 was the most appropriate marker for population genetic analysis. Full article