The 2023 MDPI Annual Report has
been released!
 
14 pages, 1068 KiB  
Article
A Patented Dietary Supplement (Hydroxy-Methyl-Butyrate, Carnosine, Magnesium, Butyrate, Lactoferrin) Is a Promising Therapeutic Target for Age-Related Sarcopenia through the Regulation of Gut Permeability: A Randomized Controlled Trial
by Mariangela Rondanelli, Clara Gasparri, Alessandro Cavioni, Claudia Sivieri, Gaetan Claude Barrile, Francesca Mansueto and Simone Perna
Nutrients 2024, 16(9), 1369; https://doi.org/10.3390/nu16091369 (registering DOI) - 30 Apr 2024
Abstract
Adequate diet, physical activity, and dietary supplementation with muscle-targeted food for special medical purposes (FSMP) or dietary supplement (DS) are currently considered fundamental pillars in sarcopenia treatment. The aim of this study is to evaluate the effectiveness of a DS (containing hydroxy-methyl-butyrate, carnosine, [...] Read more.
Adequate diet, physical activity, and dietary supplementation with muscle-targeted food for special medical purposes (FSMP) or dietary supplement (DS) are currently considered fundamental pillars in sarcopenia treatment. The aim of this study is to evaluate the effectiveness of a DS (containing hydroxy-methyl-butyrate, carnosine, and magnesium, for its action on muscle function and protein synthesis and butyrate and lactoferrin for their contribution to the regulation of gut permeability and antioxidant/anti-inflammation activity) on muscle mass (assessed by dual x-ray absorptiometry (DXA)), muscle function (by handgrip test, chair test, short physical performance battery (SPPB) test, and walking speed test), inflammation (tumor necrosis factor-alpha (TNF-a), C-reactive protein (CRP), and visceral adipose tissue (VAT)) and gut axis (by zonulin). A total of 59 participants (age 79.7 ± 4.8 years, body mass index 20.99 ± 2.12 kg/m2) were enrolled and randomly assigned to intervention (n = 30) or placebo (n = 28). The skeletal muscle index (SMI) significantly improved in the supplemented group compared to the placebo one, +1.02 (CI95%: −0.77; 1.26), p = 0.001; a significant reduction in VAT was observed in the intervention group, −70.91 g (−13.13; −4.70), p = 0.036. Regarding muscle function, all the tests significantly improved (p = 0.001) in the supplemented group compared to the placebo one. CRP, zonulin, and TNF-alpha significantly decreased (p = 0.001) in intervention, compared to placebo, −0.74 mg/dL (CI95%: −1.30; −0.18), −0.30 ng/mL (CI95%: −0.37; −0.23), −6.45 pg/mL (CI95%: −8.71; −4.18), respectively. This DS improves muscle mass and function, and the gut muscle has emerged as a new intervention target for sarcopenia. Full article
(This article belongs to the Special Issue Plant Derivatives and Bioactive Food Components for Health Promotion)
14 pages, 1123 KiB  
Article
Effects of Annealing Temperature on Bias Temperature Stress Stabilities of Bottom-Gate Coplanar In-Ga-Zn-O Thin-Film Transistors
by Yuyun Chen, Yi Shen, Yuanming Chen, Guodong Xu, Yudong Liu and Rui Huang
Coatings 2024, 14(5), 555; https://doi.org/10.3390/coatings14050555 (registering DOI) - 30 Apr 2024
Abstract
Defect annihilation of the IGZO/SiO2 layer is of great importance to enhancing the bias stress stabilities of bottom-gate coplanar thin-film transistors (TFTs). The effects of annealing temperatures (Ta) on the structure of the IGZO/SiO2 layer and the stabilities of [...] Read more.
Defect annihilation of the IGZO/SiO2 layer is of great importance to enhancing the bias stress stabilities of bottom-gate coplanar thin-film transistors (TFTs). The effects of annealing temperatures (Ta) on the structure of the IGZO/SiO2 layer and the stabilities of coplanar IGZO TFTs were investigated in this work. An atomic depth profile showed that the IGZO/SiO2 layer included an IGZO layer, an IGZO/SiO2 interfacial mixing layer, and a SiO2 layer. Higher Ta had only one effect on the IGZO layer and SiO2 layer (i.e., strengthening chemical bonds), while it had complex effects on the interfacial mixing layer—including weakening M-O bonds (M: metallic elements in IGZO), strengthening damaged Si-O bonds, and increasing O-related defects (e.g., H2O). At higher Ta, IGZO TFTs exhibited enhanced positive bias temperature stress (PBTS) stabilities but decreased negative bias temperature stress (NBTS) stabilities. The enhanced PBTS stabilities were correlated with decreased electron traps due to the stronger Si-O bonds near the interfacial layer. The decreased NBTS stabilities were related to increased electron de-trapping from donor-like defects (e.g., weak M-O bonds and H2O) in the interfacial layer. Our results suggest that although higher Ta annihilated the structural damage at the interface from ion bombardment, it introduced undesirable defects. Therefore, to comprehensively improve electrical stabilities, controlling defect generation (e.g., by using a mild sputtering condition of source/drain electrodes and oxides) was more important than enhancing defect annihilation (e.g., through increasing Ta). Full article
(This article belongs to the Special Issue Advanced Metal Oxide Films: Materials and Applications)
15 pages, 1621 KiB  
Article
Genetic Diversity of Plasmodium vivax Surface Ookinete Protein Pvs25 and Host Genes in Individuals Living along the Thai–Myanmar Border and Their Relationships with Parasite Density
by Abdifatah Abdullahi Jalei, Wanna Chaijaroenkul and Kesara Na-Bangchang
Microbiol. Res. 2024, 15(2), 693-707; https://doi.org/10.3390/microbiolres15020045 (registering DOI) - 30 Apr 2024
Abstract
Plasmodium vivax (Pv) accounts for over 50% of malaria cases in Latin America and Asia. Despite a significant reduction in Pv transmission in Thailand, the parasite remains endemic to the border areas. This study aimed to investigate the genetic diversity of [...] Read more.
Plasmodium vivax (Pv) accounts for over 50% of malaria cases in Latin America and Asia. Despite a significant reduction in Pv transmission in Thailand, the parasite remains endemic to the border areas. This study aimed to investigate the genetic diversity of the parasites and the host factors, as well as their relation to parasite density in Pvisolates, along the Thai–Myanmar border. Genetic variations in Pv markers, specifically the ookinete surface protein Pvs25, and host genes, including Toll-like receptor 6 (TLR6), TLR9, TIR Domain-containing adaptor protein (TIRAP), Toll-interacting protein (TOLLIP), Duffy antigen receptor for chemokines (DARC), and intercellular adhesion molecule 1 (ICAM-1), were investigated using polymerase chain reaction (PCR) with restriction fragment length polymorphism (RFLP). A total of 548 PCR-positive Pv samples collected from Tak and Kanchanaburi provinces during two periods (2006–2007 and 2014–2016) were included in the study. Pvs25 exhibited four haplotypes, with H1 (EGTKV) being the most prevalent in both provinces. Kanchanaburi isolates exhibited greater genetic diversity than Tak isolates. No significant deviations from neutrality were observed for Pvs25 in either area. ICAM-1 and TOLLIP s3750920 heterozygous carriers had greater median parasite densities than homozygous mutants. The TLR9 rs187084 T genotype had a significantly higher parasite density than the non-T genotype. The findings underscore the significant association between the rs3750920 C/T, rs5498 A/G, and rs187084 T genotypes and high parasite density in patients infected with Pv, highlighting their potentially critical role in malaria susceptibility. Full article
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22 pages, 6579 KiB  
Article
Numerical Simulation on the Two-Degree-of-Freedom Flow-Induced Vibration of a Submerged Floating Tunnel under Current
by Guannan Wang, Ningchuan Zhang, Guoxing Huang and Zhuowei Zhou
J. Mar. Sci. Eng. 2024, 12(5), 759; https://doi.org/10.3390/jmse12050759 (registering DOI) - 30 Apr 2024
Abstract
The submerged floating tunnel (SFT) is a novel form of transportation infrastructure for crossing deeper and wider seas. One of the primary challenges in designing SFTs is understanding their hydrodynamic response to complex environmental loads. In order to investigate the two-degree-of-freedom (2-DOF) flow-induced [...] Read more.
The submerged floating tunnel (SFT) is a novel form of transportation infrastructure for crossing deeper and wider seas. One of the primary challenges in designing SFTs is understanding their hydrodynamic response to complex environmental loads. In order to investigate the two-degree-of-freedom (2-DOF) flow-induced vibration (FIV) response of SFTs under current, a two-dimensional (2D) numerical model was developed using the Reynolds-averaged Navier–Stokes (RANS) method combined with the fourth-order Runge–Kutta method. The numerical results were validated by comparing them with the existing literature. The study then addressed the effects of coupled vibration and structural parameters, i.e., the mass ratio and natural frequency ratio, on the response and wake pattern of SFTs, numerically. The results indicated that coupled vibration had a significant impact on the SFT response at reduced velocities of Urwx ≥ 4.4. A decrease in mass ratio (m* < 1) notably amplified the 2-DOF vibration amplitudes of SFTs at Urwx ≥ 4.4, particularly for in-line vibration. Similarly, a decrease in natural frequency ratio (Rf < 1) significantly suppressed the in-line vibration of SFTs at Urwx ≥ 2.5. Therefore, for the design of SFTs, careful consideration should be given to the effect of mass ratio and natural frequency ratio on in-line vibration. Full article
(This article belongs to the Section Coastal Engineering)
31 pages, 3020 KiB  
Review
Unveiling the Potential of Natural Compounds: A Comprehensive Review on Adipose Thermogenesis Modulation
by Jaeeun Shin, Yeonho Lee, Seong Hun Ju, Young Jae Jung, Daehyeon Sim and Sung-Joon Lee
Int. J. Mol. Sci. 2024, 25(9), 4915; https://doi.org/10.3390/ijms25094915 (registering DOI) - 30 Apr 2024
Abstract
The process of adipocyte browning has recently emerged as a novel therapeutic target for combating obesity and obesity-related diseases. Non-shivering thermogenesis is the process of biological heat production in mammals and is primarily mediated via brown adipose tissue (BAT). The recruitment and activation [...] Read more.
The process of adipocyte browning has recently emerged as a novel therapeutic target for combating obesity and obesity-related diseases. Non-shivering thermogenesis is the process of biological heat production in mammals and is primarily mediated via brown adipose tissue (BAT). The recruitment and activation of BAT can be induced through chemical drugs and nutrients, with subsequent beneficial health effects through the utilization of carbohydrates and fats to generate heat to maintain body temperature. However, since potent drugs may show adverse side effects, nutritional or natural substances could be safe and effective as potential adipocyte browning agents. This review aims to provide an extensive overview of the natural food compounds that have been shown to activate brown adipocytes in humans, animals, and in cultured cells. In addition, some key genetic and molecular targets and the mechanisms of action of these natural compounds reported to have therapeutic potential to combat obesity are discussed. Full article
(This article belongs to the Special Issue Effects of Phytochemicals on Metabolic Disorders)
15 pages, 3126 KiB  
Article
Analysis of Ozone Pollution Characteristics, Meteorological Effects, and Transport Sources in Zhuzhou, China
by Bei Yan, Jia Luo, Min Zhang, Yi Zhang, Tongjue Xiao, Lu Wang, Bo Liu, Yunjuan Han, Gongxiu He, Lili Yang and Zhihong Huang
Atmosphere 2024, 15(5), 559; https://doi.org/10.3390/atmos15050559 (registering DOI) - 30 Apr 2024
Abstract
Based on the hourly surface ozone (O3) observations and meteorological data from Zhuzhou in 2021, the pollution characteristics and influencing factors of O3 in Zhuzhou were investigated in the study. In addition, the Potential Source Contribution Function (PSCF) and Concentration [...] Read more.
Based on the hourly surface ozone (O3) observations and meteorological data from Zhuzhou in 2021, the pollution characteristics and influencing factors of O3 in Zhuzhou were investigated in the study. In addition, the Potential Source Contribution Function (PSCF) and Concentration Weighted Trajectory (CWT) analysis methods were employed to analyze the transmission paths and potential pollution sources of O3 pollution in Zhuzhou. The results showed that the total number of days with O3 exceeding the standard at all monitoring stations in Zhuzhou was 142 days in 2021. The overall air quality was less affected by SO2, NO2, and CO, and the trend of O3 pollution was still increasing. The concentrations of O3, CO, and NO2 varied significantly in different months, and the variation of O3 exhibited a “double-peak” pattern, with the peak value occurring in September. The O3 concentration in urban areas was significantly higher than that in suburban areas. Meteorological conditions had a significant impact on the degree of O3 pollution in Zhuzhou. The average wind speed in Zhuzhou throughout the year was 1.7 m/s, and the prevailing wind direction in summer was southeast, with a frequency of 16%. O3 pollution was mainly transported by short-distance airflow during the over-standard periods in 2021, accounting for 37.64%. The main source of O3 pollutant was from Jiangxi Province in the east, with the shortest distance of regional transport and the highest O3 concentration. In addition, transportation from central Guangdong Province, western Jiangxi Province, and central Hubei Province also had a significant impact. Full article
19 pages, 2844 KiB  
Article
A Study of Adjacent Intersection Correlation Based on Temporal Graph Attention Network
by Pengcheng Li, Baotian Dong and Sixian Li
Entropy 2024, 26(5), 390; https://doi.org/10.3390/e26050390 (registering DOI) - 30 Apr 2024
Abstract
Traffic state classification and relevance calculation at intersections are both difficult problems in traffic control. In this paper, we propose an intersection relevance model based on a temporal graph attention network, which can solve the above two problems at the same time. First, [...] Read more.
Traffic state classification and relevance calculation at intersections are both difficult problems in traffic control. In this paper, we propose an intersection relevance model based on a temporal graph attention network, which can solve the above two problems at the same time. First, the intersection features and interaction time of the intersections are regarded as input quantities together with the initial labels of the traffic data. Then, they are inputted into the temporal graph attention (TGAT) model to obtain the classification accuracy of the target intersections in four states—free, stable, slow moving, and congested—and the obtained neighbouring intersection weights are used as the correlation between the intersections. Finally, it is validated by VISSIM simulation experiments. In terms of classification accuracy, the TGAT model has a higher classification accuracy than the three traditional classification models and can cope well with the uneven distribution of the number of samples. The information gain algorithm from the information entropy theory was used to derive the average delay as the most influential factor on intersection status. The correlation from the TGAT model positively correlates with traffic flow, making it interpretable. Using this correlation to control the division of subareas improves the road network’s operational efficiency more than the traditional correlation model does. This demonstrates the effectiveness of the TGAT model’s correlation. Full article
(This article belongs to the Special Issue Information-Theoretic Methods in Data Analytics)
14 pages, 17634 KiB  
Article
Design of Multi-Functional Bio-Safe Dental Resin Composites with Mineralization and Anti-Biofilm Properties
by Jiaojiao Yun, Michael F. Burrow, Jukka P. Matinlinna, Hao Ding, Sin Man (Rosalind) Chan, James K. H. Tsoi and Yan Wang
J. Funct. Biomater. 2024, 15(5), 120; https://doi.org/10.3390/jfb15050120 (registering DOI) - 30 Apr 2024
Abstract
This study aims to develop multi-functional bio-safe dental resin composites with capabilities for mineralization, high in vitro biocompatibility, and anti-biofilm properties. To address this issue, experimental resin composites consisting of UDMA/TEGDMA-based dental resins and low quantities (1.9, 3.8, and 7.7 vol%) of 45S5 [...] Read more.
This study aims to develop multi-functional bio-safe dental resin composites with capabilities for mineralization, high in vitro biocompatibility, and anti-biofilm properties. To address this issue, experimental resin composites consisting of UDMA/TEGDMA-based dental resins and low quantities (1.9, 3.8, and 7.7 vol%) of 45S5 bioactive glass (BAG) particles were developed. To evaluate cellular responses of resin composites, MC3T3-E1 cells were (1) exposed to the original composites extracts, (2) cultured directly on the freshly cured resin composites, or (3) cultured on preconditioned composites that have been soaked in deionized water (DI water), a cell culture medium (MEM), or a simple HEPES-containing artificial remineralization promotion (SHARP) solution for 14 days. Cell adhesion, cell viability, and cell differentiation were, respectively, assessed. In addition, the anti-biofilm properties of BAG-loaded resin composites regarding bacterial viability, biofilm thickness, and biofilm morphology, were assessed for the first time. In vitro biological results demonstrated that cell metabolic activity and ALP expression were significantly diminished when subjected to composite extracts or direct contact with the resin composites containing BAG fillers. However, after the preconditioning treatments in MEM and SHARP solutions, the biomimetic calcium phosphate minerals on 7.7 vol% BAG-loaded composites revealed unimpaired or even better cellular processes, including cell adhesion, cell proliferation, and early cell differentiation. Furthermore, resin composites with 1.9, 3.8, and 7.7 vol% BAG could not only reduce cell viability in S. mutans biofilm on the composite surface but also reduce the biofilm thickness and bacterial aggregations. This phenomenon was more evident in BAG7.7 due to the high ionic osmotic pressure and alkaline microenvironment caused by BAG dissolution. This study concludes that multi-functional bio-safe resin composites with mineralization and anti-biofilm properties can be achieved by adding low quantities of BAG into the resin system, which offers promising abilities to mineralize as well as prevent caries without sacrificing biological activity. Full article
(This article belongs to the Special Issue Functional Materials for Dental Restorations—Volume II)
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11 pages, 837 KiB  
Article
Changes in the Carotenoids of Zamia dressleri Leaves during Development
by Enrique Murillo, Veronika Nagy, Dania Menchaca, József Deli and Attila Agócs
Plants 2024, 13(9), 1251; https://doi.org/10.3390/plants13091251 (registering DOI) - 30 Apr 2024
Abstract
It has been observed that the leaves of some Zamia species undergo a kind of “reverse ripening”; that is, they change from their original brown color to green during development. We assumed that this strange color change was due to the change in [...] Read more.
It has been observed that the leaves of some Zamia species undergo a kind of “reverse ripening”; that is, they change from their original brown color to green during development. We assumed that this strange color change was due to the change in carotenoid composition, so we followed the changes for several weeks. The detailed carotenoid composition and content at different stages of development of the leaves was determined with HPLC-DAD focusing on the changes in red and yellow carotenoids. The total and relative amounts of red and yellow carotenoids were determined simultaneously from one measurement from a saponified and/or unsaponified extract. At the beginning of development, the concentration of red carotenoids was higher than that of the yellow ones; it decreased drastically until 22 days and continued to decrease slowly until they completely disappeared. The concentration of yellow carotenoids decreased at the beginning as well, but after 22 days it started to increase. The amount of red carotenoids started to decrease when the leaflet stopped growing. Lutein is the main component in old leaflets, which is not a red carotenoid precursor. Red carotenoids can always be found in their esterified form in the leaves. These findings support the hypothesis that red and yellow carotenoid accumulation are independent and probably have different functions in the leaflet. The strange color change was explained based on the compartmentalization of red and yellow carotenoids and on the changing activity of the enzyme capsanthin–capsorubin synthase responsible for the synthesis of red carotenoids capsorubin and capsanthin. Full article
(This article belongs to the Section Phytochemistry)
19 pages, 3328 KiB  
Article
Dimension Prediction and Microstructure Study of Wire Arc Additive Manufactured 316L Stainless Steel Based on Artificial Neural Network and Finite Element Simulation
by Yanyan Di, Zhizhen Zheng, Shengyong Pang, Jianjun Li and Yang Zhong
Micromachines 2024, 15(5), 615; https://doi.org/10.3390/mi15050615 (registering DOI) - 30 Apr 2024
Abstract
The dimensional accuracy and microstructure affect the service performance of parts fabricated by wire arc additive manufacturing (WAAM). Regulating the geometry and microstructure of such parts presents a challenge. The coupling method of an artificial neural network and finite element (FE) is proposed [...] Read more.
The dimensional accuracy and microstructure affect the service performance of parts fabricated by wire arc additive manufacturing (WAAM). Regulating the geometry and microstructure of such parts presents a challenge. The coupling method of an artificial neural network and finite element (FE) is proposed in this research for this purpose. Back-propagating neural networks (BPNN) based on optimization algorithms were established to predict the bead width (BW) and height (BH) of the deposited layers. Then, the bead geometry was modeled based on the predicted dimension, and 3D FE heat transfer simulation was performed to investigate the evolution of temperature and microstructure. The results showed that the errors in BW and BH were less than 6%, and the beetle antenna search BPNN model had the highest prediction accuracy compared to the other models. The simulated melt pool error was less than 5% with the experimental results. The decrease in the ratio of the temperature gradient and solidification rate induced the transition of solidified grains from cellular crystals to columnar dendrites and then to equiaxed dendrites. Accelerating the cooling rate increased the primary dendrite arm spacing and δ-ferrite content. These results indicate that the coupling model provides a pathway for regulating the dimensions and microstructures of manufactured parts. Full article
21 pages, 24644 KiB  
Article
WaveSegNet: An Efficient Method for Scrap Steel Segmentation Utilizing Wavelet Transform and Multiscale Focusing
by Jiakui Zhong, Yunfeng Xu and Changda Liu
Mathematics 2024, 12(9), 1370; https://doi.org/10.3390/math12091370 (registering DOI) - 30 Apr 2024
Abstract
Scrap steel represents a sustainable and recyclable resource, instrumental in diminishing carbon footprints and facilitating the eco-friendly evolution of the steel sector. However, current scrap steel recycling faces a series of challenges, such as high labor intensity and occupational risks for inspectors, complex [...] Read more.
Scrap steel represents a sustainable and recyclable resource, instrumental in diminishing carbon footprints and facilitating the eco-friendly evolution of the steel sector. However, current scrap steel recycling faces a series of challenges, such as high labor intensity and occupational risks for inspectors, complex and diverse sources of scrap steel, varying types of materials, and difficulties in quantifying and standardizing manual visual inspection and rating. Specifically, we propose WaveSegNet, which is based on wavelet transform and a multiscale focusing structure for scrap steel segmentation. Firstly, we utilize wavelet transform to process images and extract features at different frequencies to capture details and structural information in the images. Secondly, we introduce a mechanism of multiscale focusing to further enhance the accuracy of segmentation by extracting and perceiving features at different scales. Through experiments conducted on the public Cityscapes dataset and scrap steel datasets, we have found that WaveSegNet consistently demonstrates superior performance, achieving the highest scores on the mIoU metric. Particularly notable is its performance on the real-world scrap steel dataset, where it outperforms other segmentation algorithms with an average increase of 3.98% in mIoU(SS), reaching 69.8%, and a significant boost of nearly 5.98% in mIoU(MS), achieving 74.8%. These results underscore WaveSegNet’s exceptional capabilities in processing scrap steel images. Additionally, on the publicly available Cityscapes dataset, WaveSegNet shows notable performance enhancements compared with the next best model, Segformer. Moreover, with its modest parameters and computational demands (34.1 M and 322 GFLOPs), WaveSegNet proves to be an ideal choice for resource-constrained environments, demonstrating high computational efficiency and broad applicability. These experimental results attest to the immense potential of WaveSegNet in intelligent scrap steel rating and provide a new solution for the scrap steel recycling industry. These experimental results attest to the immense potential of WaveSegNet in intelligent scrap steel rating and provide a new solution for the scrap steel recycling industry. Full article
(This article belongs to the Special Issue Artificial Intelligence and Data Science)
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14 pages, 4022 KiB  
Article
Differential Colonization and Mucus Ultrastructure Visualization in Bovine Ileal and Rectal Organoid-Derived Monolayers Exposed to Enterohemorrhagic Escherichia coli
by Minae Kawasaki and Yoko M. Ambrosini
Int. J. Mol. Sci. 2024, 25(9), 4914; https://doi.org/10.3390/ijms25094914 (registering DOI) - 30 Apr 2024
Abstract
Enterohemorrhagic Escherichia coli (EHEC) is a critical public health concern due to its role in severe gastrointestinal illnesses in humans, including hemorrhagic colitis and the life-threatening hemolytic uremic syndrome. While highly pathogenic to humans, cattle, the main reservoir for EHEC, often remain asymptomatic [...] Read more.
Enterohemorrhagic Escherichia coli (EHEC) is a critical public health concern due to its role in severe gastrointestinal illnesses in humans, including hemorrhagic colitis and the life-threatening hemolytic uremic syndrome. While highly pathogenic to humans, cattle, the main reservoir for EHEC, often remain asymptomatic carriers, complicating efforts to control its spread. Our study introduces a novel method to investigate EHEC using organoid-derived monolayers from adult bovine ileum and rectum. These polarized epithelial monolayers were exposed to EHEC for four hours, allowing us to perform comparative analyses between the ileal and rectal tissues. Our findings mirrored in vivo observations, showing a higher colonization rate in the rectum compared with the ileum (44.0% vs. 16.5%, p < 0.05). Both tissues exhibited an inflammatory response with increased expression levels of TNF-a (p < 0.05) and a more pronounced increase of IL-8 in the rectum (p < 0.01). Additionally, the impact of EHEC on the mucus barrier varied across these gastrointestinal regions. Innovative visualization techniques helped us study the ultrastructure of mucus, revealing a net-like mucin glycoprotein organization. While further cellular differentiation could enhance model accuracy, our research significantly deepens understanding of EHEC pathogenesis in cattle and informs strategies for the preventative measures and therapeutic interventions. Full article
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18 pages, 312 KiB  
Review
A Narrative Review of the Efficacy of Interventions for Emotional Dysregulation, and Underlying Bio–Psycho–Social Factors
by Thomas Easdale-Cheele, Valeria Parlatini, Samuele Cortese and Alessio Bellato
Brain Sci. 2024, 14(5), 453; https://doi.org/10.3390/brainsci14050453 (registering DOI) - 30 Apr 2024
Abstract
In this narrative, comprehensive, and updated review of the literature, we summarize evidence about the effectiveness of interventions aimed at reducing emotion dysregulation and improving emotion regulation in children, adolescents, and adults. After introducing emotion dysregulation and emotion regulation from a theoretical standpoint, [...] Read more.
In this narrative, comprehensive, and updated review of the literature, we summarize evidence about the effectiveness of interventions aimed at reducing emotion dysregulation and improving emotion regulation in children, adolescents, and adults. After introducing emotion dysregulation and emotion regulation from a theoretical standpoint, we discuss the factors commonly associated with emotion regulation, including neurobiological and neuropsychological mechanisms, and the role of childhood adverse experiences and psycho–social factors in the onset of emotion dysregulation. We then present evidence about pharmacological and non-pharmacological interventions aiming at improving emotion dysregulation and promoting emotion regulation across the lifespan. Although our review was not intended as a traditional systematic review, and the search was only restricted to systematic reviews and meta-analyses, we highlighted important implications and provided recommendations for clinical practice and future research in this field. Full article
18 pages, 1460 KiB  
Article
Endophytic Capacity of Entomopathogenic Fungi in a Pasture Grass and Their Potential to Control the Spittlebug Mahanarva spectabilis (Hemiptera: Cercopidae)
by Michelle O. Campagnani, Alexander Machado Auad, Rogério Martins Maurício, Ana Paula Madureira, Mauroni Alves Cangussú, Luiz Henrique Rosa, Marcelo Francisco A. Pereira, Mayco Muniz, Sebastião Rocha O. Souza, Natany Brunelli M. Silva, Ana Carolina Rios Silva and Wellington Garcia Campos
Agronomy 2024, 14(5), 943; https://doi.org/10.3390/agronomy14050943 (registering DOI) - 30 Apr 2024
Abstract
Pests in pastures have compromised the production of biomass for feeding livestock herds. Many strategies have been applied to sustainably solve this problem. One viable and innovative technique is the delivery of entomopathogenic fungi through endophytes. Therefore, this study aimed to (i) evaluate [...] Read more.
Pests in pastures have compromised the production of biomass for feeding livestock herds. Many strategies have been applied to sustainably solve this problem. One viable and innovative technique is the delivery of entomopathogenic fungi through endophytes. Therefore, this study aimed to (i) evaluate the endophytic capacity of two entomopathogenic fungi, Fusarium multiceps UFMGCB 11443 and Metarhizium anisopliae UFMGCB 11444, in Urochloa brizantha [(Hochst. ex A. Rich.) Stapf] (Poaceae) cultivar ‘Marundu’) via foliar inoculation or seed treatment, and (ii) measure their efficiency in controlling Mahanarva spectabilis Distant, 1909 (Hemiptera: Cercopidae) in U. brizantha. In the greenhouse, the fungi colonized the tissues of U. brizantha plants when inoculated via foliar spraying or seed treatment. The fungi F. multiceps and M. anisopliae caused 88% and 97.1% epizootic effects via seed inoculation, respectively, and 100% epizootic effects via foliar inoculation. In the field, the lowest fungal dose of 0.5 kg/ha had the same effect as a fourfold greater dose, with a >86% decrease in insect pest infestation observed. In summary, the fungi F. multiceps and M. anisopliae have endophytic effects and can effectively control M. spectabilis in U. brizantha pastures. Full article
(This article belongs to the Special Issue Biological Pest Control in Agroecosystems)
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34 pages, 1699 KiB  
Article
On the Accurate Estimation of Information-Theoretic Quantities from Multi-Dimensional Sample Data
by Manuel Álvarez Chaves, Hoshin V. Gupta, Uwe Ehret and Anneli Guthke
Entropy 2024, 26(5), 387; https://doi.org/10.3390/e26050387 (registering DOI) - 30 Apr 2024
Abstract
Using information-theoretic quantities in practical applications with continuous data is often hindered by the fact that probability density functions need to be estimated in higher dimensions, which can become unreliable or even computationally unfeasible. To make these useful quantities more accessible, alternative approaches [...] Read more.
Using information-theoretic quantities in practical applications with continuous data is often hindered by the fact that probability density functions need to be estimated in higher dimensions, which can become unreliable or even computationally unfeasible. To make these useful quantities more accessible, alternative approaches such as binned frequencies using histograms and k-nearest neighbors (k-NN) have been proposed. However, a systematic comparison of the applicability of these methods has been lacking. We wish to fill this gap by comparing kernel-density-based estimation (KDE) with these two alternatives in carefully designed synthetic test cases. Specifically, we wish to estimate the information-theoretic quantities: entropy, Kullback–Leibler divergence, and mutual information, from sample data. As a reference, the results are compared to closed-form solutions or numerical integrals. We generate samples from distributions of various shapes in dimensions ranging from one to ten. We evaluate the estimators’ performance as a function of sample size, distribution characteristics, and chosen hyperparameters. We further compare the required computation time and specific implementation challenges. Notably, k-NN estimation tends to outperform other methods, considering algorithmic implementation, computational efficiency, and estimation accuracy, especially with sufficient data. This study provides valuable insights into the strengths and limitations of the different estimation methods for information-theoretic quantities. It also highlights the significance of considering the characteristics of the data, as well as the targeted information-theoretic quantity when selecting an appropriate estimation technique. These findings will assist scientists and practitioners in choosing the most suitable method, considering their specific application and available data. We have collected the compared estimation methods in a ready-to-use open-source Python 3 toolbox and, thereby, hope to promote the use of information-theoretic quantities by researchers and practitioners to evaluate the information in data and models in various disciplines. Full article
(This article belongs to the Special Issue Approximate Entropy and Its Application)
14 pages, 5170 KiB  
Article
Development of a New Vertical Dynamic Model of a Rail Vehicle for the Analysis of Ride Comfort
by Yusuf Çati, Mesut Düzgün and Frédéric Etienne Kracht
Appl. Sci. 2024, 14(9), 3848; https://doi.org/10.3390/app14093848 (registering DOI) - 30 Apr 2024
Abstract
The rail vehicle industry wants to produce vehicles with higher speeds, to maintain and increase its market share. However, when the speed of the vehicle increases, it may have an undesirable effect on ride comfort, in terms of ride dynamics. Recent developments towards [...] Read more.
The rail vehicle industry wants to produce vehicles with higher speeds, to maintain and increase its market share. However, when the speed of the vehicle increases, it may have an undesirable effect on ride comfort, in terms of ride dynamics. Recent developments towards lighter and faster vehicles make the problem of ride comfort at higher speeds increasingly important. Focusing on the behavior of flexible rather than rigid body behavior should not be neglected when designing long and light car bodies. There are several approaches to incorporate body flexibility in multibody simulations and they have some superiorities and weaknesses. In this study, an efficient and accurate vertical dynamic model for the ride comfort analysis is developed and implemented in a commercial object-oriented modeling (OOM) software Dymola (2015 FD01) which uses the open-source code Modelica. This model includes car body flexibility with the assembling of a rigid body approach. The developed model is compared to a three-dimensional vehicle model in the commercial Vampire software (Pro V5.50) at different velocities. For the vertical ride comfort analysis, the ISO 2631-1 standard was used for both the developed model and the three-dimensional model. The results are presented as acceleration history and awrms—weighted r.m.s (root mean square) of accelerations—as required by the standard. The developed model has shown its feasibility in terms of its efficiency and accuracy for the vertical ride comfort analysis. The accuracy of the model is evidenced by the fact that the car body vibration level at high speeds shows minor differences compared to the results of the Vampire, which is a validated commercial software in the area of rail vehicle dynamics. The approach involving the assembly of rigid bodies is applied for the first time for high-speed trains in dynamical modelling, with flexible car bodies for ride comfort analysis. Furthermore, it can be used for parametrical studies focusing on ride comfort, thereby offering a quite beneficial framework for addressing the challenges of ride comfort analysis in high-speed rail vehicles. Improvements for and analyses of other aspects are also possible, since the optimization and other useful libraries are readily available in Dymola/Modelica. Full article
(This article belongs to the Topic Vehicle Dynamics and Control)
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23 pages, 6120 KiB  
Article
Analysis of Wind Field Response Characteristics of Tethered Balloon Systems
by Ce Pang, Zeqing He, Kaiyin Song and Shenghong Cao
Aerospace 2024, 11(5), 360; https://doi.org/10.3390/aerospace11050360 (registering DOI) - 30 Apr 2024
Abstract
Tethered balloon systems encounter various complex wind field environments during flight. To investigate the conditions under which the system can operate safely and smoothly, a longitudinal dynamic model for tethered balloon systems is established. The model incorporates a streamlined balloon shape with its [...] Read more.
Tethered balloon systems encounter various complex wind field environments during flight. To investigate the conditions under which the system can operate safely and smoothly, a longitudinal dynamic model for tethered balloon systems is established. The model incorporates a streamlined balloon shape with its aerodynamic center at the body’s center. Steady-state aerodynamic force coefficients are calculated through simulations and fitted to a function based on the angle of attack within a specified range. The complex cable model is simplified using the lumped mass method, considering the influence of branch cables on the main node position. Experimental results from windless oscillation tests on scaled tethered balloon systems are compared with numerical solutions obtained using the dynamic model under the same conditions, validating the feasibility of the model for simulating different wind field scenarios. Finally, the motion characteristics of tethered balloon systems in different wind fields are analyzed. The numerical simulation results show that in a horizontal step wind field, the cable tension and cable inclination angle increase with the wind speed, and the slower the wind field changes, the shorter the time required for system stabilization. Updrafts greatly increase the likelihood of balloon escape, while downdrafts greatly increase the likelihood of the system making contact with the ground. The findings of this study can provide a basis for selecting suitable wind field conditions and issuing risk warnings for tethered balloon systems. Full article
19 pages, 806 KiB  
Article
Digital Health Support: Current Status and Future Development for Enhancing Dialysis Patient Care and Empowering Patients
by Bernard Canaud, Andrew Davenport, Hélène Leray-Moragues, Marion Morena-Carrere, Jean Paul Cristol, Jeroen Kooman and Peter Kotanko
Toxins 2024, 16(5), 211; https://doi.org/10.3390/toxins16050211 (registering DOI) - 30 Apr 2024
Abstract
Chronic kidney disease poses a growing global health concern, as an increasing number of patients progress to end-stage kidney disease requiring kidney replacement therapy, presenting various challenges including shortage of care givers and cost-related issues. In this narrative essay, we explore innovative strategies [...] Read more.
Chronic kidney disease poses a growing global health concern, as an increasing number of patients progress to end-stage kidney disease requiring kidney replacement therapy, presenting various challenges including shortage of care givers and cost-related issues. In this narrative essay, we explore innovative strategies based on in-depth literature analysis that may help healthcare systems face these challenges, with a focus on digital health technologies (DHTs), to enhance removal and ensure better control of broader spectrum of uremic toxins, to optimize resources, improve care and outcomes, and empower patients. Therefore, alternative strategies, such as self-care dialysis, home-based dialysis with the support of teledialysis, need to be developed. Managing ESKD requires an improvement in patient management, emphasizing patient education, caregiver knowledge, and robust digital support systems. The solution involves leveraging DHTs to automate HD, implement automated algorithm-driven controlled HD, remotely monitor patients, provide health education, and enable caregivers with data-driven decision-making. These technologies, including artificial intelligence, aim to enhance care quality, reduce practice variations, and improve treatment outcomes whilst supporting personalized kidney replacement therapy. This narrative essay offers an update on currently available digital health technologies used in the management of HD patients and envisions future technologies that, through digital solutions, potentially empower patients and will more effectively support their HD treatments. Full article
(This article belongs to the Special Issue Kidney Replacement Therapy by Hemodialysis: 21st Century Challenges)
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15 pages, 2221 KiB  
Article
Field Emission from Carbon Nanotubes on Titanium Nitride-Coated Planar and 3D-Printed Substrates
by Stefanie Haugg, Luis-Felipe Mochalski, Carina Hedrich, Isabel González Díaz-Palacio, Kristian Deneke, Robert Zierold and Robert H. Blick
Nanomaterials 2024, 14(9), 781; https://doi.org/10.3390/nano14090781 (registering DOI) - 30 Apr 2024
Abstract
Carbon nanotubes (CNTs) are well known for their outstanding field emission (FE) performance, facilitated by their unique combination of electrical, mechanical, and thermal properties. However, if the substrate of choice is a poor conductor, the electron supply towards the CNTs can be limited, [...] Read more.
Carbon nanotubes (CNTs) are well known for their outstanding field emission (FE) performance, facilitated by their unique combination of electrical, mechanical, and thermal properties. However, if the substrate of choice is a poor conductor, the electron supply towards the CNTs can be limited, restricting the FE current. Furthermore, ineffective heat dissipation can lead to emitter–substrate bond degradation, shortening the field emitters’ lifetime. Herein, temperature-stable titanium nitride (TiN) was deposited by plasma-enhanced atomic layer deposition (PEALD) on different substrate types prior to the CNT growth. A turn-on field reduction of up to 59% was found for the emitters that were generated on TiN-coated bulk substrates instead of on pristine ones. This observation was attributed exclusively to the TiN layer as no significant change in the emitter morphology could be identified. The fabrication route and, consequently, improved FE properties were transferred from bulk substrates to free-standing, electrically insulating nanomembranes. Moreover, 3D-printed, polymeric microstructures were overcoated by atomic layer deposition (ALD) employing its high conformality. The results of our approach by combining ALD with CNT growth could assist the future fabrication of highly efficient field emitters on 3D scaffold structures regardless of the substrate material. Full article
(This article belongs to the Special Issue The Research Related to Nanomaterial Cold Cathode II)
17 pages, 39075 KiB  
Article
Early-Stage ISC Fault Detection for Ship Lithium Batteries Based on Voltage Variance Analysis
by Yu Gu, Haishen Ni and Yuwei Li
Machines 2024, 12(5), 303; https://doi.org/10.3390/machines12050303 (registering DOI) - 30 Apr 2024
Abstract
With the progressive development of new energy technologies, high-power lithium batteries have been widely used in ship power systems due to their high-power density and low environmental pollution, and they have gradually become one of their main propulsion energy sources. However, the large-scale [...] Read more.
With the progressive development of new energy technologies, high-power lithium batteries have been widely used in ship power systems due to their high-power density and low environmental pollution, and they have gradually become one of their main propulsion energy sources. However, the large-scale deployment of lithium batteries has also brought a series of safety problems to ship operations, especially the battery internal short circuit (ISC). Battery ISC faults are very hidden and unpredictable at the initial stage and often fail to be detected in time, ultimately leading to overheating, fire or even an explosion of the ship’s power system. Based on this, this paper proposes a fast and accurate method for early-stage ISC fault location and detection of lithium batteries. Initially, voltage variations across the lithium battery packs are quantified using curvilinear Manhattan distances to pinpoint faulty battery units. Subsequently, the localized characteristics of voltage variance among adjacent batteries are leveraged to detect an early-stage ISC fault. Simulation results indicate that the proposed method can quickly and accurately locate the position of 5 Ω, 10 Ω and 15 Ω ISC faulty batteries within the battery pack, as well as detect the abnormal batteries in a timely manner with considerable sensitivity and reliability. Full article
(This article belongs to the Special Issue Data-Driven Fault Diagnosis for Machines and Systems)
31 pages, 7247 KiB  
Article
A Spatiotemporal Probabilistic Graphical Model Based on Adaptive Expectation-Maximization Attention for Individual Trajectory Reconstruction Considering Incomplete Observations
by Xuan Sun, Jianyuan Guo, Yong Qin, Xuanchuan Zheng, Shifeng Xiong, Jie He, Qi Sun and Limin Jia
Entropy 2024, 26(5), 388; https://doi.org/10.3390/e26050388 (registering DOI) - 30 Apr 2024
Abstract
Spatiotemporal information on individual trajectories in urban rail transit is important for operational strategy adjustment, personalized recommendation, and emergency command decision-making. However, due to the lack of journey observations, it is difficult to accurately infer unknown information from trajectories based only on AFC [...] Read more.
Spatiotemporal information on individual trajectories in urban rail transit is important for operational strategy adjustment, personalized recommendation, and emergency command decision-making. However, due to the lack of journey observations, it is difficult to accurately infer unknown information from trajectories based only on AFC and AVL data. To address the problem, this paper proposes a spatiotemporal probabilistic graphical model based on adaptive expectation maximization attention (STPGM-AEMA) to achieve the reconstruction of individual trajectories. The approach consists of three steps: first, the potential train alternative set and the egress time alternative set of individuals are obtained through data mining and combinatorial enumeration. Then, global and local potential variables are introduced to construct a spatiotemporal probabilistic graphical model, provide the inference process for unknown events, and state information about individual trajectories. Further, considering the effect of missing data, an attention mechanism-enhanced expectation-maximization algorithm is proposed to achieve maximum likelihood estimation of individual trajectories. Finally, typical datasets of origin-destination pairs and actual individual trajectory tracking data are used to validate the effectiveness of the proposed method. The results show that the STPGM-AEMA method is more than 95% accurate in recovering missing information in the observed data, which is at least 15% more accurate than the traditional methods (i.e., PTAM-MLE and MPTAM-EM). Full article
(This article belongs to the Section Signal and Data Analysis)
18 pages, 16136 KiB  
Article
Learning Event Representations for Zero-Shot Detection via Dual-Contrastive Prompting
by Jiaxu Li, Bin Ge, Hao Xu, Peixin Huang and Hongbin Huang
Mathematics 2024, 12(9), 1372; https://doi.org/10.3390/math12091372 (registering DOI) - 30 Apr 2024
Abstract
Zero-shot event detection aims to involve the automatic discovery and classification of new events within unstructured text. Current zero-shot event detection methods have not considered addressing the problem more effectively from the perspective of improving event representations. In this paper, we propose dual-contrastive [...] Read more.
Zero-shot event detection aims to involve the automatic discovery and classification of new events within unstructured text. Current zero-shot event detection methods have not considered addressing the problem more effectively from the perspective of improving event representations. In this paper, we propose dual-contrastive prompting (COPE) model for learning event representations to address zero-shot event detection, which leverages prompts to assist in generating event embeddings using a pretrained language model, and employs a contrastive fusion approach to capture complex interaction information between trigger representations and sentence embeddings to obtain enhanced event representations. Firstly, we introduce a sample generator to create ordered contrastive sample sequences with varying degrees of similarity for each event instance, aiding the model in better distinguishing different types of events. Secondly, we design two distinct prompts to obtain trigger representations and event sentence embeddings separately. Thirdly, we employ a contrastive fusion module, where trigger representations and event sentence embeddings interactively fuse in vector space to generate the final event representations. Experiments show that our model is more effective than the most advanced methods. Full article
25 pages, 2473 KiB  
Article
Experimental Study on Anisotropic Mechanical Characteristics of Shale under Triaxial Loading
by Qian Dong, Jia Kang, Jinshan Sun, Jingjie Li and Zhen Zhang
Appl. Sci. 2024, 14(9), 3849; https://doi.org/10.3390/app14093849 (registering DOI) - 30 Apr 2024
Abstract
Shale is composed of a rock matrix and bedding planes with a layered structure, resulting in significant anisotropy in its mechanical properties. In order to study the anisotropic mechanical properties of shale, the shale samples were prepared in different orientations with respect to [...] Read more.
Shale is composed of a rock matrix and bedding planes with a layered structure, resulting in significant anisotropy in its mechanical properties. In order to study the anisotropic mechanical properties of shale, the shale samples were prepared in different orientations with respect to the bedding planes, and the composition and microstructure of shale were first analyzed by X-ray diffractometer (XRD) and scanning electron microscope (SEM), and then the uniaxial and triaxial compression experiment on shale samples with five different bedding angles (the angle between the loading direction and the normal direction of the bedding planes, 0°, 30°, 45°, 60°, and 90°) were conducted under five confining pressures (0, 10, 20, 30, and 40 MPa), respectively; meanwhile, the acoustic emission (AE) test was carried out in the uniaxial test. The results indicate that the mechanical properties and parameters of shale have obvious anisotropy, and the AE characteristics of shale samples with different bedding angles are significantly different during uniaxial loading. Furthermore, the compressive strength and elastic modulus of the shale samples first decrease and then increase with the increase in the bedding angle under different confining pressures. Moreover, according to the anisotropic grade of compressive strength, the shale has moderate anisotropy. In addition, the failure mode of the shale samples is also anisotropic, and varies with the bedding angle and confining pressure. Full article

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