MYB transcription factors (TFs) have been shown to be important regulators of plant growth and development, and the R2R3−MYB family plays an important role in the regulation of plant primary and secondary metabolism, growth, and development. In this study, the PmMYB45 gene sequence was successfully cloned from Pinus massoniana, with an open reading frame of 1476 bp, encoding 491 amino acids. The results of subcellular localization and transcriptional self-activation showed that the gene was localized in the nucleus and was self-activating. qPCR showed that the highest expression of PmMYB45 was found in stems. PmMYB45 can promote lignin synthesis when overexpressed in tobacco. These results suggest that PmMYB45 promotes lignin synthesis by regulating the lignin biosynthesis pathway. These findings improve our understanding of the mechanism of lignin biosynthesis in P. massoniana and provide a basis for molecular breeding functions. It also provides insights into the role of the MYB transcription factor family in lignin accumulation. Full article

According to parental reports, about 10% of children are believed to be allergic to at least one antibiotic, leading to the prescription of second line medications. This incurs higher costs, results in less effective treatments, and contributes to global concern of antibiotic resistance. De-labeling programs could mitigate these problems. The primary objectives of this study were to assess the proportion of children that tolerate the suspected antibiotic well through allergy testing and, secondly, to examine which information in their medical history correlates with a positive test result. Children with a history of antibiotic allergy were categorized into high- and low-risk groups for immediate allergic reaction. The latter underwent oral provocation testing (OPT), while the high-risk group underwent the test only after negative skin tests (STs). In total, 76.8% of children tolerated the tested antibiotic well. Among children with positive OPT, two (8.0%) had to receive adrenaline for symptom resolution. Children who had exhibited suspected symptoms within one hour after antibiotic administration, and those with a history of asthma or food allergy, had an increased risk of positive allergic testing (p < 0.05). In conclusion, the adoption of a standardized protocol for an antibiotic allergy de-labeling program is essential for every allergy department. Full article

Graphene has achieved mass production via various preparative routes and demonstrated its uniqueness in many application fields for its intrinsically high electron mobility and thermal conductivity. However, graphene faces limitations in assembling macroscopic structures because of its hydrophobic property. Therefore, balancing high crystal quality and good aqueous dispersibility is of great importance in practical applications. Herein, we propose a tape-wrapping strategy to electrochemically fabricate water-dispersible graphene (w-Gr) with both excellent dispersibility (~4.5 mg/mL, stable over 2 months), and well-preserved crystalline structure. A large production rate (4.5 mg/min, six times faster than previous electrochemical methods), high yield (65.4% ≤5 atomic layers) and good processability are demonstrated. A mechanism investigation indicates that the rational design of anode configuration to ensure proper oxidation, deep exfoliation and unobstructed mass transfer is responsible for the high efficiency of this strategy. This simple yet efficient electrochemical method is expected to promote the scalable preparation and applications of graphene. Full article

In the vast field of organic functional materials, viologens are widely recognized as an extremely versatile family of substances, due in part to the possibility of extending conjugation between the terminal pyridinium rings, for instance through the insertion of additional aromatic moieties. In this work, a new, extended viologen with a thiophene core and two acetylene bonds is presented. It was synthesized through a straightforward route, using well-established Sonogashira coupling reactions, and its optical properties were investigated by UV–visible absorption and fluorescence spectroscopy, revealing a very interesting material for diverse fluorescence-related applications. Full article

(1) Background: To better understand the dynamic characteristics of a ball bearing with an elastic ring squeeze film damper (ERSFD) under sudden unbalance, a novel dynamic model was established by fully considering the coupling between the ERSFD, bearing outer ring (the journal), rotor, and disc (loading bearing); (2) Methods: An improved secant method was developed to determine the initial eccentricity values of the bearing’s outer ring and the disc. The dynamic response of the outer ring under different speed ratios, damping ratios, and mass ratios was solved using the variable-step Runge–Kutta method; (3) Results: In comparison, a low-speed ratio, high damping ratio, and low mass ratio were more conducive to suppressing the bearing vibration. When the imbalance was suddenly introduced, the displacement amplitude of the eccentricity, transmissibility, amplitude–frequency response, and the radius of the outer ring center locus increased; (4) Conclusions: This work provides a reference for further studying the nonlinear vibration of rolling bearings coupled with an ERSFD. Full article

The COVID-19 pandemic disrupted families and displaced individuals. For migrant workers, these disruptions and displacements exacerbated the state-imposed constraints on family formation. But how did high-skilled and high-wage immigrants, presumably immune from these challenges, provide care to and receive care from families during the pandemic? Based on 33 in-depth interviews with high-skilled Asian Indian immigrants in the USA during the pandemic, we note disruptions in their care to and from families. These disruptions reveal a persistent pattern of dispersion in immigrant families which leads to what we call “dispersed care.” By “dispersed care” we identify the effects of various state-imposed immigration laws and policies, which force immigrants to divide and allocate care among multiple fragments of their families in home and host countries. Dispersed care affects immigrant workers’ professional output, forcing them to make difficult choices between their career and care commitments. To unsettle the assumed homogeneity of high-skilled “Asian Indians,” we choose participants at diverse intersections of their migration pathways—naturalized US citizens, permanent US residents, and temporary visa holders or nonimmigrants. While naturalized US citizens and permanent residents have better resources to maintain transnational family ties than nonimmigrants, all of them face the intersectional challenges of dispersed care. Full article

Broilers are particularly sensitive to heat stress, which can impair growth, and lower conversion efficiency and survival rates. Under a climate change scenario, maintaining optimal thermal conditions within broiler houses becomes more complex and energy-intensive. Climate change can worsen air quality issues inside broiler houses by increasing the concentration of harmful gases, and proper mechanical ventilation systems are essential for diluting and removing these gases. The present study aimed to develop and validate a model for the ideal broiler housing strategy by applying the Paraconsistent Annotated Evidential Logic Eτ. A database from four broiler houses in a commercial farm, rearing 157,700 birds from the 1st to the 42nd day of growth, was used in the research. All environmental data were recorded weekly inside the houses, and on day 42, flock mortality, overall feed-to-gain ratio, and body weight were calculated and registered. The Cohen’s Kappa statistics for each environmental parameter classification compared to the paraconsistent classification. Results indicated that temperature shows good agreement, relative humidity shows slight agreement, air velocity presents a good agreement, CO₂ concentration has a slight agreement, and NH₃ concentration is classified by slight agreement. The environmental and productivity variables as a function of the broiler age using the extreme True paraconsistent state indicate the model validation. The paraconsistent analysis presented the ideal scenario for broilers’ growth, maintaining the environmental variables level within a particular threshold and providing greater profit to broiler farmers. Full article

Full-depth reclamation with Portland cement (FDR-PC) represents an innovative cold recycling technology for pavements, holding significant promise due to its capacity to reuse deteriorated pavement base layers. This paper investigates the key factors influencing the strength properties of FDR-PC. The results indicate that, compared to the static compaction method, the vibratory compaction method yields cold-recycled mixtures with higher maximum dry density and unconfined compressive strength (UCS). Increasing the cement content and base-to-surface ratio, extending the curing time, and raising the curing temperature all contribute to enhancing UCS. Furthermore, increasing the base-to-surface ratio and cement content enhances both indirect tensile strength and flexural strength. An approximate linear correlation exists between indirect tensile strength and UCS, as well as between flexural strength and UCS. The strength characteristics of FDR-PC were comprehensively characterized in this study, providing effective verification of its applicability. Full article

Microplastics as micropollutants are widely spread in aquatic areas that can have a toxic effect on aquatic life. To reduce the potential risk they pose, it is essential to detect the microplastics and the source of the contamination of the environment. Here, we designed and developed a droplet-based microfluidic impedance flow cytometer for in situ detection of microplastics in water. Impedance spectroscopy enables the direct measurement of the electrical features of microplastics as they move in water, allowing for sizing and identification of concentration. To show the feasibility of the developed method, pure and functionalized polystyrene beads ranging from 500 nm to 6 μm in four size groups and different concentrations were used. Focusing on three different frequencies (4.4 MHz, 11 MHz, and 22.5 MHz), the changes in the signal phase at frequencies of 4.4 MHz and 11 MHz are a strong indicator of microplastic presence. In addition, the functionalized microplastics showed different magnitudes of the measured signal phase than the pure ones. A k-nearest neighbors classification model demonstrated our developed system’s impressive 97.4% sensitivity in accurately identifying microplastics based on concentration. The equivalent circuit model revealed that the double-layer capacity of water droplets is significantly impacted by the presence of the microplastics. Our findings show the potential of droplet-based microfluidic impedance flow cytometry as a practical method for detecting microplastics in water. Full article

Vernonia cinerea (L.) Less. is a perennial herbaceous plant found mainly in tropical areas, particularly in Southeast Asia, South America, and India. Various parts of V. cinerea have traditionally been used in folk medicine to treat several diseases, such as malaria, fever, and liver diseases. V. cinerea has so far yielded about 92 secondary metabolites. The majority of these are sesquiterpene lactones, but triterpenes, flavonoids, steroids, phenolics, and other compounds are present as well. V. cinerea crude extracts reportedly exhibit anti-inflammatory, antiprotozoal, antidiabetic, anticancer, antimicrobial, antioxidant, and renoprotective activities. This study aims to provide the latest up-to-date information on the botanical characterization, distribution, traditional uses, phytochemistry, and pharmacological activity of V. cinerea. Information on V. cinerea was thoroughly reviewed. The literature published between 1950 and 2024 was compiled through online bibliographic databases, including SciFinder, Web of Science, Google Scholar, PubMed, ScienceDirect, Springer Link, Wiley, and the MDPI online library. The keywords used for the literature search included Vernonia cinerea (L.) Less. and the synonyms Cyanthillium cinereum (L.) H.Rob., Conyza cinerea L., and various others. Full article

Virtual reality is a technological development that, among others, has revolutionized Earth sciences. Its advantages include an opportunity to examine places otherwise difficult or impossible to access and it may also become an important component of education, fostering a better understanding of processes and landforms, geohazard awareness, and an appreciation of geoheritage. This paper reports on the GeoVT project, which aims to create a platform to build and disseminate Virtual Field Trips (VFTs) focused on geomorphology, natural hazards associated with geomorphological processes, and geoheritage sites. To put the GeoVT project in context, an overview of applications of VR in geosciences is provided. This paper subsequently proceeds with a presentation of the project and the GeoVT Authoring application, which is an innovative platform designed to help teachers and students, followed by brief presentations of a number of VFTs developed within the project. They address themes such as fluvial landforms and valley development, coastal landforms, evidence of past glaciation, coastal erosion, wildfire effects, mud volcanoes, and landslides. Full article

The wastewaters of nejayote and food waste leachate are polluting effluents with a high load of organic matter that cause great problems when discharged to water receptors. In this work, we investigated the treatment of nejayote wastewaters and food waste leachate for the production of microalgae Scenedesmus acutus and Haematococcus pluvialis. For Scenedesmus acutus, treatment with 10% food waste leachate and 90% growth medium resulted in a concentration of 5.34 g/L in 20 days (μmax = 0.16/day). Meanwhile, 10% nejayote and 90% medium growth produced 4.45 g/L at 20 days (μmax = 0.13/d). A significant reduction of up to 82.6% ammonium, 84.1% orthophosphate, and 87.25% COD was also observed between the different treatments. For Haematococcus pluvialis, the treatment of 90% food waste leachate and 10% growth medium produced a concentration of 4.73 g/L at 6 days (μmax = 0.71/day), while the mixture of 25% najayote, 25% food waste leachate, and 50% growth medium produced a concentration of 5.5 g/L at 20 days (μmax = 0.25/dat). A reduction of up to 97.8% ammonia, 97.4% orthophosphate, and 73.19% COD was also recorded. These findings demonstrated the potential to cultivate microalgae and extract biomolecules for commercial purposes. Full article

An existential vacuum is experienced as a kind of crisis that one can cope with using his/her strengths. The related literature suggests that the important determinants of coping with existential emptiness include positive emotional and personality resources, and among these—Curiosity Behaviors. The purpose of this study is to describe the role of curiosity as an important factor in relation to emotional resources in individuals experiencing an existential vacuum. A survey was conducted using online tools (n = 484). The hypotheses about the direct and indirect relationships between Curiosity Behaviors and existential vacuum were tested using multiple regression analyses and mediations. The study involved a sample of adult participants representing the general population. The participants completed five questionnaires, the first one focusing on Curiosity Behaviors, such as seeking out novel and challenging experiences and engagement in activities that capture one’s attention (The Curiosity and Exploration Inventory), and the other tools being the Multidimensional Existential Meaning Scale, the Emotional Regulation Questionnaire, the Flourishing Scale, and the Depression Anxiety Stress Scale. The results indicate that Curiosity Behaviors characteristically negatively predict existential vacuum. In addition, other variables, such as Flourishing, seem to be useful in explaining the relationships between these factors. Flourishing along with Curiosity increases a sense of Meaning in Life. Our results present evidence showing the importance of Curiosity Behaviors in coping with the existential vacuum. Full article

In this study, we report on the isolation, purification, and anti-inflammatory evaluation of compounds from the plant species Ageratina pichinchensis. Using open-column chromatography, 11 known compounds were purified, which chemical structures were elucidated by nuclear magnetic resonance techniques (1D and 2D). All compounds were evaluated in an in vitro model of RAW 264.7 mouse macrophage cells, measuring the nitric oxide inhibition to determine the anti-inflammatory effect. The compound betuletol 3-O-β-glucoside (11) inhibited nitric oxide with a half-maximal inhibitory concentration (IC₅₀) of 75.08 ± 3.07% at 75 µM; additionally, it inhibited the secretion of interleukin 6 (IL-6) and activation of the nuclear factor (NF-kβ). These results suggest that the anti-inflammatory effect attributed to A. pichinchensis species is promoted by compound 11, which could be considered a potential anti-inflammatory agent by suppressing the expression of NF-kβ target genes, such as those involved in the proinflammatory pathway and inducible nitric oxide synthase (iNOS). Full article

Background: Blood vessels have the Windkessel effect and are involved in blood circulation. The breakdown of this mechanism is also involved in the pathogenesis of heart failure (HF); however, the relationship between vascular dysfunction and HF prognosis is not fully understood. Methods: We evaluated 214 patients hospitalized for HF at our institution who underwent a cardio–ankle vascular index (CAVI), which evaluates vascular function, between January 2012 and July 2018. To investigate factors (including CAVI) associated with major adverse cardiac events (MACE) during 1 year after patients with HF were discharged, we evaluated clinical profiles, blood tests, chest X-P, 12-lead electrocardiography, and transthoracic echocardiographic findings. MACE was defined as cardiovascular death or readmission for HF. Results: The severity of HF between the MACE and non-MACE was not significantly different. Previous HF and chronic kidney disease were significantly more common in the MACE group. CAVI and % mean atrial pressure in the MACE group were statistically higher than those in the non-MACE group. The cardiac shadow as shown by chest X-P and left ventricular size in the MACE group were significantly bigger, and HF preserved ejection fraction (EF) (EF > 50%) was significantly more common in the MACE group. In multivariate analysis, CAVI was an independent predictive factor for the occurrence of MACE (model 1; hazard ratio (HR): 1.33, 95% confidence interval (CI): 1.05–1.68, p = 0.018; model 2; HR: 1.31, 95% CI: 1.07–1.60, p = 0.009). Conclusions: Because high CAVI is associated with poor prognosis of HF, these patients require more careful treatment. Full article

Feminist reflections on the sexual division of labour have given rise to a body of knowledge on the ethics of care from different disciplines, including philosophy, in which outstanding contributions to the topic have been formulated. This approach is applicable to the analysis of any phenomenon and particularly that of disasters. As various investigations have highlighted, the consequences on the population throughout all of a disaster’s phases (prevention, emergency, and reconstruction) require an analysis of differentiated vulnerabilities based on gender and other identity categories, such as social class, ethnicity, age, disability, sexual identity, etc. The interrelation between all these variables gives rise to differentiated impacts that cannot be ignored in catastrophic contexts, where survival and sustaining life are at stake, so care becomes a central issue. Research on the topic has also identified that, along with the analysis of social vulnerability, we must consider the capacity for agency, both individual and collective, where care is once again of vital importance. Considering the gender approach and its multiple intersections is thus a fundamental theoretical-practical proposal for the study of disasters from philosophy, as it implies an unavoidable epistemic, ontological, and ethical reflection in the face of risk reduction. Full article

This study investigates the effects of the gulf stream (GS) on sea-level oscillations across various time scales and assesses the performance of a coastal and estuarine model nested within a global model in simulating these variations. It aims to improve boundary conditions to simulate sea-level oscillations more accurately by considering the influence of GS flow. An inverse correlation is observed between observed sea-level oscillation and GS flow, which becomes more pronounced over longer time scales. Using Delft3D, a high-resolution coastal and estuarine model is developed to simulate circulation dynamics in the central Indian River Lagoon (IRL), FL, and adjacent coastal areas on the Florida east coast. The model is nested into the HYCOM (Hybrid Coordinate Ocean Model), and meteorological forcings are derived from the NARR (North American Regional Reanalysis) model. The model demonstrates satisfactory performance across key parameters, including tide, salinity, water temperature, and currents. However, there remains a noticeable difference between the modeled and observed data. To address this, the model is executed with modified flow boundary conditions at eastern boundary nodes, integrating HYCOM tide, and observing low-frequency sea-level variations. The implementation of the new boundary conditions results in an improved simulation of sea-level oscillations. This study presents the conceptual framework and detailed methodologies employed in the creation of a high-resolution model tailored for estuarine and coastal areas nested into global models capable of satisfactorily simulating sea-level oscillations even when the global model does not represent GS effects. Full article

Sprayers’ exposure to glyphosate was analyzed through detection of its biomarker in spot urine biological monitoring, and the health risk was assessed using the biomatrix model. Urine samples were collected from 15 sprayers after spraying, and the glyphosate concentration was determined by using the DLLME-HPLC method with a UV detector. The calibration curve for glyphosate was linear in the range of 0.4–100 µg/L, while the limits of detection and quantification were 0.1 µg/L and 0.4 µg/L, respectively. The human health risk was estimated using the hazard quotient (HQ) and the biomatrix of risk assessment. The internal dose ranged from 0.0001 to 0.0021 mg/kg b.w./day. The non-cancer HQ showed no potential health risk concerns (HQ < 1). The biomatrix of health risk assessment, based on urinary glyphosate concentration, exhibited a strong correlation with the health risk matrix model. This correlation was determined by considering the likelihood of exposure, calculated from the quantity of glyphosate used and the usage of personal protective equipment (r = 0.854, p < 0.001). Although low risk was observed in sprayers, proper PPE use and the application of more knowledge are required. The simplified health risk assessment can be used for easy self-assessment of risk in preventive action regarding health risk awareness among sprayers. Full article

Carbon geological storage (CGS) is one of the key processes in carbon capture and storage (CCS) technologies, which are used to reduce CO₂ emissions and achieve carbon-neutrality and net-zero emissions in developing countries. In Thailand, the Mae Moh basin is a potential site for implementing CGS due to the presence of a structural trap that can seal the CO₂ storage formation. However, the cost of CGS projects needs to be subsidized by selling carbon credits in order to reach the project breakeven. Therefore, this paper estimates the economic components of a CGS project in the Mae Moh basin by designing the well completion and operating parameters for CO₂ injection. The capital costs and operating costs of the process components were calculated, and the minimum carbon credit cost required to cover the total costs of the CGS project was determined. The results indicate that the designed system proposes an operating gas injection rate of 1.454 MMscf/day, which is equivalent to 29,530 tCO₂e per year per well. Additionally, the minimum carbon credit cost was estimated to be USD 70.77 per tCO₂e in order to achieve breakeven for the best case CGS project, which was found to be much higher than the current market price of carbon credit in Thailand, at around USD 3.5 per tCO₂e. To enhance the economic prospects of this area, it is imperative to promote a policy of improving the cost of carbon credit for CGS projects in Thailand. Full article

Influenza viruses cause highly contagious respiratory diseases that cause millions of deaths worldwide. Rapid detection of influenza viruses is essential for accurate diagnosis and the initiation of appropriate treatment. We developed a loop-mediated isothermal amplification and lateral flow assay (LAMP-LFA) capable of simultaneously detecting influenza A and influenza B. Primer sets for influenza A and influenza B were designed to target conserved regions of segment 7 and the nucleoprotein gene, respectively. Optimized through various primer set ratios, the assay operated at 62 °C for 30 min. For a total of 243 (85 influenza A positive, 58 influenza B positive and 100 negative) nasopharyngeal swab samples, the performance of the influenza A/B multiplex LAMP-LFA was compared with that of the commercial Allplex^TM Respiratory Panel 1 assay (Seegene, Seoul, Korea). The influenza A/B multiplex LAMP-LFA demonstrated a specificity of 98% for the non-infected clinical samples, along with sensitivities of 94.1% for the influenza A clinical samples and 96.6% for the influenza B clinical samples, respectively. The influenza A/B multiplex LAMP-LFA showed high sensitivity and specificity, indicating that it is reliable for use in a low-resource environment. Full article

Sperm capacitation involves biochemical and physiological changes that enable sperm to fertilize the oocyte. It can be induced in vitro under controlled conditions that simulate the environment of the oviduct. While extensively studied in mammals, its approach in lizards remains absent. Understanding the mechanisms that ensure reproduction is essential for advancing the implementation of assisted reproductive technologies in this group. We aimed to perform a sperm analysis to determine if capacitation-related changes were induced after incubation with capacitating media. Fifteen males of Sceloporus torquatus were collected during the early stage of the reproductive season. The sperm were isolated from the seminal plasma and then diluted up to a volume of 150 μL using BWW medium to incubate with 5% CO₂ at 30 °C for a maximum duration of 3 h. A fraction was retrieved hourly for ongoing sperm assessment. The sperm analysis included assessments of its motility, viability, the capacitation status using the chlortetracycline (CTC) assay, and the acrosome integrity with the lectin binding assay to detect changes during incubation. We found that total motility was maintained up to 2 h post incubation, after which it decreased. However, sperm viability remained constant. From that moment on, we observed a transition to a deeper and less symmetrical flagellar bending in many spermatozoa. The CTC assay indicated a reduction in the percentage of sperm showing the full (F) pattern and an increase in those exhibiting the capacitated (B) and reactive (RA) patterns, accompanied by an elevation in the percentage of damaged acrosomes as revealed by the lectin binding assay. In mammals, these changes are often associated with sperm capacitation. Our observations support the notion that this process may also occur in saurian. While sperm analysis is a valuable method for assessing certain functional changes, additional approaches are required to validate this process. Full article

The intricate process of planning production, involving product life cycle management and the synthesis of manufacturing information, is crucial for coherence in manufacturing. Manufacturing companies, operating in a high-mix, low-volume production environment, integrate production planning with management to focus on production processes, emphasizing high-quality, rapid product delivery. This includes material item planning to anticipate future demands and ensure sufficient raw material and finished product quantities, considering purchasing, production, and sales capacities. This study explores the electro technical sector, specifically a manufacturing entity specializing in low-voltage plastic cable distribution boxes. It scrutinizes the vital role of seasonal data cleaning in optimizing production planning, with a targeted focus on three products. The implementation of a chase demand strategy is related to capacity planning, taking into account the change in production capacity linked to demand over time. The problem in implementing this strategy is related to the fluctuating level of quality due to changes in demand for specified products. Full article