2,3-dihydro-5,6,7,8-tetranitro-1,4-benzodioxine (TNBD), molecular formula =C8H4N4O10, is a completely nitrated aromatic ring 1,4-benzodioxane derivative. The convenient method of TNBD synthesis was developed (yield = 81%). The detailed structure of this compound was investigated by X-ray crystallography. The results of the thermal analysis (TG) obtained with twice re-crystallized material revealed the onset at 240 °C (partial sublimation started) and melting at 286 °C. The investigated material degraded completely at 290–329 °C. The experimental density of 1.85 g/cm³ of TNBD was determined by X-ray crystallography. The spectral properties of TNBD (NMR, FT-IR and Raman) were explored. The detonation properties of TNBD calculated by the EXPLO 5 code were slightly superior in comparison to standard high-energy material—tetryl (detonation velocity of TNBD—7727 m/s; detonation pressure—278 kbar; and tetryl—7570 m/s and 226.4 kbar at 1.614 g/cm³, or 260 kbar at higher density at 1.71 g/cm³. The obtained preliminary results might suggest TNBD can be a potential thermostable high-energy and -density material (HEDM). Full article

In aeolian sand movement, the vertical distribution of sand particle size is intricately linked to sand flux, wind–sand flow field and dune development. In the present study, the distribution characteristics of sand grains in four particle size ranges at nine heights were investigated through sand blowing tests at five different reference wind speeds. The correlation between sand particle size and wind speed indicates that when the particle size was ≥0.35 mm, there was a linear variation of mass percentage with wind speed. When the particle size was <0.35 mm, when Z ≤ 0.15 m, a linear variation of mass percentage with wind speed was found; when Z > 0.15 m, an exponential modification in mass percentage with wind velocity was observed for sand grains falling within this specific range of particle sizes. The correlation between sand particle size and height indicates that when the reference wind speed was ≥15 m/s, the mass percentage of sand particles varied linearly with height. When the reference wind speed was ≤13.5 m/s, the mass percentage of sand grains with particle size in the 0.25–0.35mm range increases first and then decreases with increasing height. The present results can provide a reference for subsequent research on the aerodynamic characteristics of wind–sand flow fields and on the mechanism of dune formation. Full article

Metabolomics has proven to be a sensitive tool for monitoring biochemical processes in cell culture. It enables multi-analysis, clarifying the correlation between numerous metabolic pathways. Together with other analysis, it thus provides a global view of a cell’s physiological state. A comprehensive analysis of molecular changes is also required in the case of mesenchymal stem cells (MSCs), which currently represent an essential portion of cells used in regenerative medicine. Reproducibility and correct measurement are closely connected to careful metabolite extraction, and sample preparation is always a critical point. Our study aimed to compare the efficiencies of four harvesting and six extraction methods. Several organic reagents (methanol, ethanol, acetonitrile, methanol–chloroform, MTBE) and harvesting approaches (trypsinization vs. scraping) were tested. We used untargeted nuclear magnetic resonance spectroscopy (NMR) to determine the most efficient method for the extraction of metabolites from human adherent cells, specifically human dermal fibroblasts adult (HDFa) and dental pulp stem cells (DPSCs). A comprehensive dataset of 29 identified and quantified metabolites were determined to possess statistically significant differences in the abundances of several metabolites when the cells were detached mechanically to organic solvent compared to when applying enzymes mainly in the classes of amino acids and peptides for both types of cells. Direct scraping to organic solvent is a method that yields higher abundances of determined metabolites. Extraction with the use of different polar reagents, 50% and 80% methanol, or acetonitrile, mostly showed the same quality. For both HDFa and DPSC cells, the MTBE method, methanol–chloroform, and 80% ethanol extractions showed higher extraction efficiency for the most identified and quantified metabolites Thus, preparation procedures provided a cell sample processing protocol that focuses on maximizing extraction yield. Our approach may be useful for large-scale comparative metabolomic studies of human mesenchymal stem cell samples. Full article

Biodiversity conservation entails not only the preservation of specific taxa but also genetic diversity. Despite the crucial role of molecular data in freshwater fish conservation management, there is a scarcity of information regarding the genetic diversity of Luciobarbus Heckel, 1843 (Actinopterygii, Cyprinidae) populations in the Aral system. Therefore, the primary aim of this study was to provide genetic information on two native species of the Luciobarbus genus found in the Aral system: L. conocephalus (Kessler, 1872) and L. brachycephalus (Kessler, 1872). These species, like many others in the Aral system, confront the imminent threat of extinction due to system alterations. However, genetic studies on these species at the nuclear level are challenging because Luciobarbus is an allotetraploid genus. Consequently, genetic investigations thus far have focused mainly on sequencing mitochondrial genes due to their haploid nature. This study has successfully developed fifteen new polymorphic microsatellite loci, which can prove to be valuable for population genetics, conservation, and other pertinent research on these species. Full article

We investigated the electronic structure of Mg-, Si-, and Zn-doped four-faceted [001]- and [110]-oriented SnO₂ nanowires using first-principles calculations based on the linear combination of atomic orbitals (LCAO) method. This approach, employing atomic-centered Gaussian-type functions as a basis set, was combined with hybrid density functional theory (DFT). Our results show qualitative agreement in predicting the formation of stable point defects due to atom substitutions on the surface of the SnO₂ nanowire. Doping induces substantial atomic relaxation in the nanowires, changes in the covalency of the dopant–oxygen bond, and additional charge redistribution between the dopant and nanowire. Furthermore, our calculations reveal a narrowing of the band gap resulting from the emergence of midgap states induced by the incorporated defects. This study provides insights into the altered electronic properties caused by Mg, Si, and Zn doping, contributing to the further design of SnO₂ nanowires for advanced electronic, optoelectronic, photovoltaic, and photocatalytic applications. Full article

Aksu City, located in the southern region of Xinjiang, China, holds the position of being the fifth largest city in Xinjiang. It holds significant ecological importance as a vital functional region for the management of desertification in China. To safeguard the ecological security of Xinjiang and preserve the ecological stability of Aksu City, it is crucial to examine the relationship between ecological service value and ecological risk, as well as the geographical and temporal changes in land use characteristics in Aksu City. This study examines the evolutionary characteristics and spatial correlation between ecological service value and ecological risk in Aksu City, using Aksu City as a case study. The analysis is based on five periods of land use data from 2000, 2005, 2010, 2015, and 2020. The study revealed the spatial and temporal patterns of landscape ecological risk and ecosystem service value in Aksu City from 2000 to 2020 using the landscape pattern index, ecological service value estimation, and ecological risk index. In addition, the study explored the interrelationship between ecological service value and ecological risk. The findings indicated that: (1) Bare land constituted the predominant land use category in Aksu City, accounting for over 81% of the total land use transfer over a 20-year period, encompassing a total area of 459.83 km². (2) The total ecological service value (ESV) in the area experienced a decline of CNY 3.41 × 10⁸ within the study’s time frame, exhibiting a decrease rate of 6.73%. Notably, grass and shrubland emerged as the primary contributor to the ESV, accounting for 33.25% of the total. (3) The ecological risk index (ERI) in Aksu City, within the period of 2000–2020, showed an increase in the interval from 0.2686 to 0.2877. The results indicated a decline in the overall ecological condition. The ecological risk level in Aksu City from 2000 to 2020 was dominated by lower and medium ecological risks. (4) Moran’s I values in Aksu City between 2000 and 2020 ranged from 0.428 to 0.443, which suggested a positive spatial correlation between ESV and ERI in the study area. The primary factor contributing to the heightened ecological risk in the study region was predominantly attributed to human activities such as urban expansion, agricultural production, and overgrazing. Full article

The advancements of Internet of Things (IoT) technologies have enabled the implementation of smart and wearable sensors, which can be employed to provide older adults with affordable and accessible continuous biophysiological status monitoring. The quality of such monitoring data, however, is unsatisfactory due to excessive noise induced by various disturbances, such as motion artifacts. Existing methods take advantage of summary statistics, such as mean or median values, for denoising, without taking into account the biophysiological patterns embedded in data. In this research, a functional data analysis modeling method was proposed to enhance the data quality by learning individual subjects’ diurnal heart rate (HR) patterns from historical data, which were further improved by fusing newly collected data. This proposed data-fusion approach was developed based on a Bayesian inference framework. Its effectiveness was demonstrated in an HR analysis from a prospective study involving older adults residing in assisted living or home settings. The results indicate that it is imperative to conduct personalized healthcare by estimating individualized HR patterns. Furthermore, the proposed calibration method provides a more accurate (smaller mean errors) and more precise (smaller error standard deviations) HR estimation than raw HR and conventional methods, such as the mean. Full article

The current psychedelic renaissance intersects with Christian practices in two key ways. First, as psychedelic-assisted therapy (PAT) becomes more common, Christians undergoing therapeutic medical treatment may seek outside support for integrating into their religious lives mystical experiences that occur during psychedelic sessions. Second, with increasing legal access to psychedelics, more Christians may explore their spiritual potential outside of a medical context, either individually with spiritual guides or collectively in organized retreats. Many will have mystical encounters related to the Divine. Whether the experience involves the overwhelming presence or absence of the Divine, these Christians, too, will seek integration support. This essay argues that the Bible can serve as a rich source for such integration, because it contains significant material about mystical experiences marked by altered states of consciousness. First, I summarize the importance of the psychedelic renaissance, especially the scientific studies being conducted, as it relates to Christian practices of spiritual formation. Second, I explore new work being conducted by biblical scholars regarding embodied religious experiences with the Divine (and others), including mystical experiences. Third, I consider the Apostle Paul’s embodied mystical experience, with special attention to 2 Corinthians 12:1–10, as one example of biblical material that might intersect with or inform psychedelic mystical encounters that contemporary Christians might experience (whether in a medical therapeutic or non-medical spiritual formation setting). Finally, I indicate directions for further research and discussion. Full article

The long-term goal of this research is the development of a stand-alone tactile device for the communication of speech for persons with profound sensory deficits as well as for applications for persons with intact hearing and vision. Studies were conducted with a phoneme-based tactile display of speech consisting of a 4-by-6 array of tactors worn on the dorsal and ventral surfaces of the forearm. Unique tactile signals were assigned to the 39 English phonemes. Study I consisted of training and testing on the identification of 4-phoneme words. Performance on a trained set of 100 words averaged 87% across the three participants and generalized well to a novel set of words (77%). Study II consisted of two-way messaging between two users of TAPS (TActile Phonemic Sleeve) for 13 h over 45 days. The participants conversed with each other by inputting text that was translated into tactile phonemes sent over the device. Messages were identified with an accuracy of 73% correct in conjunction with 82% of the words. Although rates of communication were slow (roughly 1 message per minute), the results obtained with this ecologically valid procedure represent progress toward the goal of a stand-alone tactile device for speech communication. Full article

The objective of this work was to gain insight into the operating conditions that affect the efficiency of ultrasound-assisted extraction (UAE) parameters to achieve the best recovery of bioactive compounds from broccoli leaf and floret byproducts. Therefore, total phenolic content (TPC) and the main sulfur bioactive compounds (sulforaphane (SFN) and glucosinolates (GLSs)) were assayed. Distilled water was used as solvent. For each byproduct type, solid/liquid ratio (1:25 and 2:25 g/mL), temperature (25, 40, and 55 °C), and extraction time (2.5, 5, 7.5, 10, 15, and 20 min) were the studied variables to optimize the UAE process by using a kinetic and a cubic regression model. TPC was 12.5-fold higher in broccoli leaves than in florets, while SFN was from 2.5- to 4.5-fold higher in florets regarding the leaf’s extracts obtained from the same plants, their precursors (GLS) being in similar amounts for both plant tissues. The most efficient extraction conditions were at 25 °C, ratio 2:25, and during 15 or 20 min according to the target phytochemical to extract. In conclusion, the type of plant tissue and used ratio significantly influenced the extraction of bioactive compounds, the most efficient UAE parameters being those with lower energy consumption. Full article

Virtual simulators of embedded systems and analyses of student surveys regarding their use at the early stage of the process of learning embedded systems, are presented in this article. The questionnaires were prepared in the Polish language, and the answers were automatically translated into English using two publicly available translators. The results of users’ experiences and feelings related to the use of virtual simulators are shown on the basis of detected sentiment using three chosen analysis methods: the Flair NLP library, the Pattern library, and the BERT NLP model. The results of the selected sentiment detection methods were compared and related to users reference answers, which gives information about the methods quality of the methods and their possible use in the automated review analysis process. This paper comprises detailed sentiment analysis results with a broader statistical approach for each question. Based on the students feedback and sentiment analysis, a new version of the TMSLAB v.2 virtual simulator was created. Full article

In the sixth generation (6G) era, intelligent machine network (IMN) applications, such as intelligent transportation, require collaborative machines with communication, sensing, and computation (CSC) capabilities. This article proposes an integrated communication, sensing, and computation (ICSAC) framework for 6G to achieve the reciprocity among CSC functions to enhance the reliability and latency of communication, accuracy and timeliness of sensing information acquisition, and privacy and security of computing to realize the IMN applications. Specifically, the sensing and communication functions can merge into unified platforms using the same transmit signals, and the acquired real-time sensing information can be exploited as prior information for intelligent algorithms to enhance the performance of communication networks. This is called the computing-empowered integrated sensing and communications (ISAC) reciprocity. Such reciprocity can further improve the performance of distributed computation with the assistance of networked sensing capability, which is named the sensing-empowered integrated communications and computation (ICAC) reciprocity. The above ISAC and ICAC reciprocities can enhance each other iteratively and finally lead to the ICSAC reciprocity. To achieve these reciprocities, we explore the potential enabling technologies for the ICSAC framework. Finally, we present the evaluation results of crucial enabling technologies to show the feasibility of the ICSAC framework. Full article

Nb₃Sn is emerging as one of the focal points in superconducting radio frequency (SRF) research, owing to its excellent superconducting properties. These properties hold significant possibilities for cost reduction and the miniaturization of accelerators. In this paper, we report the recent efforts of the Shanghai Advanced Research Institute (SARI) in fabricating high-performance Nb₃Sn superconducting cavities using the vapor diffusion method. This includes the construction of a Nb₃Sn coating system with dual evaporators and the test results of 1.3 GHz single-cell coated cavities. The coated samples were characterized, and the growth state of the Nb₃Sn films was analyzed. The first coated superconducting cavity was tested at both 4.4 K and 2 K, with different cooldown rates passing through the Nb₃Sn critical temperatures. The causes of Sn droplet spot defect formation on the surface of the first cavity were analyzed, and such defects were eliminated in the coating of the second cavity by controlling the evaporation rate. This study provides a reference for the preparation of high-performance Nb₃Sn-coated cavities using the vapor diffusion method, including the setup of the coating system, the comprehension of the vapor diffusion process, and the test conditions. Full article

DNA methylation is an important mechanism for epigenetic modifications that have been shown to be associated with responses to plant development. Previous studies found that inverted Populus yunnanensis cuttings were still viable and could develop into complete plants. However, the growth status of inverted cuttings was weaker than that of upright cuttings, and the sprouting time of inverted cuttings was later than that of upright cuttings. There is currently no research on DNA methylation patterns in inverted cuttings of Populus yunnanensis. In this study, we detected genome-wide methylation patterns of stem tips of Populus yunnanensis at the early growth stage and the rapid growth stage by Oxford Nanopore Technologies (ONT) methylation sequencing. We found that the methylation levels of CpG, CHG, CHH, and 6mA were 41.34%, 33.79%, 17.27%, and 12.90%, respectively, in the genome of inverted poplar cuttings, while the methylation levels of the four methylation types were higher in the genome of upright poplar cuttings than in inverted cuttings, 41.90%, 34.57%, 18.09%, and 14.11%, suggesting important roles for DNA methylation in poplar cells. In all comparison groups, CpG-type methylation genes in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway were annotated to pathways associated with carbon metabolism, ribosome biogenesis in eukaryotes, glycolysis/gluconeogenesis, pyruvate metabolism, and mRNA detection pathways, suggesting that different biological processes are activated in upright and inverted cuttings. The results show that methylation genes are commonly present in the poplar genome, but only a few of them are involved in the regulation of expression in the growth and development of inverted cuttings. From this, we screened the DET2 gene for significant differences in methylation levels in upright or inverted cuttings. The DET2 gene is a key gene in the Brassinolide (BRs) synthesis pathway, and BRs have an important influence on the growth and development process of plants. These results provide important clues for studying DNA methylation patterns in P. yunnanensis. Full article

Reducing greenhouse gas emissions is a crucial challenge in urban areas characterized by high energy consumption and reduced exposure to nature. In this context, the urban green system could play a pivotal role. In the literature, scholars have analyzed both the ability of species-specific and layout-specific green infrastructure to increase carbon sequestration and the best location sites for new green infrastructure to increase the provision of overall ecosystem services. There is a lack of studies helping green urban planners and designers choose where and which green infrastructure to implement based on vegetation species-specific performance and the local carbon emissions of city components. This paper uses tree inventory data from a medium-sized city in central Italy (Perugia) to develop a spatial analysis of urban park performance in carbon sequestration. Then, the method evaluates the carbon emission of a public city building to generate a spatialized balance between building demand and tree supply to support local decisions about the best locations for new green infrastructure and the choice between species. The paper contributes to GIS-based tools that vary the recommended location sites and species for new green infrastructure based on the demanded ecosystem service. Full article

Comprehension of the long-term effects of irrigation on basic soil characteristics and quality is essential for sustainable land management and agricultural production, particularly in arid regions where water availability is limited. This study aimed to investigate long-term irrigation effects on soil quality, soil organic carbon (SOC), and nitrogen (N) stocks in the arid lands of Egypt. Seventy soil samples were collected and analyzed to determine various soil properties. A soil quality index (SQI), SOC, and N stocks were computed. ANOVA and PCA analyses were used to identify significant differences between alluvial soils in the southwest part of the investigated area and coastal marine soils in the northeast of the study area. The results demonstrated that most of the studied soil parameters had significantly greater values in alluvial compared to coastal marine soils. Long-term irrigation led to an 8.00% increase in SOC and 7.22% increase in N stocks compared to coastal marine soils production. Furthermore, a 39.53% increase was found in the SQI upon long-term irrigation practice. These results suggest that shifting from rain-fed in coastal marine areas to irrigated production systems in alluvial fields can improve soil quality, SOC, and N stocks. Therefore, further studies are required to investigate the impact of additional factors, such as irrigation method and salinity status of sub-surface soil layers, to enhance agricultural productivity and sustainable land use. Full article

This study aimed to further understand psychological abuse in sports and contribute to the development of elite sports and athletes’ persistent performance by identifying the causal effects of psychological abuse on elite athletes’ exercise stress, job satisfaction, intention to quit exercise, and quality of life (QOL). Data were collected from 363 elite South Korean male athletes (ages ≥ 20 years) from August to September 2023. The independent variable for comparative analysis was the presence or absence of psychological abuse in elite male athletes by coaches. The participants were divided into two groups: a non-abuse-experienced group (Group 1) and an abuse-experienced group (Group 2). Participants’ demographic and athletic background information (e.g., career and sport) were also collected. This study showed that the three factors (exercise stress, intention to quit exercise, and QOL) were higher in Group 2 than in Group 1. These findings provide a meaningful analysis of the impact of psychological abuse on the mental health, persistence, and overall QOL of elite male athletes that can be used to develop countermeasures and policies against psychological abuse that threatens the mental health of elite athletes. Full article

The most common type of periodontal disease is chronic periodontitis, an inflammatory condition caused by pathogenic bacteria in subgingival plaque. The aim of our study was the development of a real-time PCR test as a diagnostic tool for the detection and differentiation of five periodontopathogenic bacteria, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Treponema denticola, in patients with periodontitis. We compared the results of our in-house method with the micro-IDent^® semiquantitative commercially available test based on the PCR hybridization method. DNA was isolated from subgingival plaque samples taken from 50 patients and then analyzed by both methods. Comparing the results of the two methods, they show a specificity of 100% for all bacteria. The sensitivity for A. actinomycetemcomitans was 97.5%, for P. gingivalis 96.88%, and for P. intermedia 95.24%. The sensitivity for Tannerella forsythia and T. denticola was 100%. The Spearman correlation factor of two different measurements was 0.976 for A. actinomycetemcomitans, 0.967 for P. gingivalis, 0.949 for P. intermedia, 0.966 for Tannerella forsythia, and 0.917 for T. denticola. In conclusion, the in-house real-time PCR method developed in our laboratory can provide information about relative amount of five bacterial species present in subgingival plaque in patients with periodontitis. It is likely that such a test could be used in dental diagnostics in assessing the efficacy of any treatment to reduce the bacterial burden. Full article

The measurement of soil water content is a very important factor in plant cultivation, both from an economic and ecological point of view. Proper estimation of moisture content not only allows for proper yields but can also contribute to ecologically appropriate use of fresh water, of which the world’s resources are limited. It is important, for example, that the moisture content in the root area of plants is optimal for their growth, while over-watering can result in losses in the form of water, which seeps below the root layer and is lost. The novel, inexpensive electronic meter for measuring soil moisture is presented in the article. The meter, based on a capacitive method, uses an optimization algorithm to calculate soil electrical permeability and a simplified new formula between soil electrical permeability and volumetric moisture content. Moreover, by using two high-frequency signals for measurements, it is possible not only to estimate moisture content but also soil conductivity. Both readings obtained from the meter not only allow for rational management of crop optimization for economic reasons but are also important for environmental protection. In addition, the inexpensive meter, based on the principle of operation presented, can be made as an IoT module, which allows for its wide application. Full article

Coordination compounds, like iron(II) triazole complexes, exhibit spin crossover (SCO) behavior at around room temperature. Therefore, they are interesting for a variety of possible applications, and it is convenient to integrate them into polymers. Due to a reduction of the iron content and thus also ⁵⁷Fe content in the sample through integration in polymers, Mössbauer measurements are only possible with greater difficulty or very long measurement times without expensive enrichment of the samples with ⁵⁷Fe. So, other ways of improving the Mössbauer signal for these composite materials are necessary. Therefore, we pressed these composite materials to improve the Mössbauer spectra. In this study, we synthesized an iron(II) triazole spin crossover complex and an electrospun polymer complex composite nanofiber material including the same complex. For both products, Mössbauer measurements were performed at room temperature before and after using a press to show that the complex composite is not harmed through pressing. We investigate the influence of the pressing impact on the Mössbauer measurements in the context of measurement statistics and the measured signals. We show that pressing is not connected to any changes in the sample regarding the spin and oxidation state. We present that pressing improves the statistics of the Mössbauer measurements significantly. Furthermore, we use SEM measurements and PXRD to investigate whether or not the obtained fiber mats are destroyed in the pressing process. Full article