The 2023 MDPI Annual Report has
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12 pages, 467 KiB  
Article
Introducing the Occupational Health and Safety Potential Midpoint Impact Indicator in Social Life Cycle Assessment
by Georgios Archimidis Tsalidis
Sustainability 2024, 16(9), 3844; https://doi.org/10.3390/su16093844 (registering DOI) - 03 May 2024
Abstract
Occupational health and safety (OSH) is crucial for sustainable development, recognized by corporations, the European Union, and Sustainable Development Goals. This study introduces a characterization model for OSH in the social life cycle assessment (S-LCA) to support the quantification of OHS along product [...] Read more.
Occupational health and safety (OSH) is crucial for sustainable development, recognized by corporations, the European Union, and Sustainable Development Goals. This study introduces a characterization model for OSH in the social life cycle assessment (S-LCA) to support the quantification of OHS along product supply chains and sustainable decision making. The characterization model aims to provide a practical approach for assessing OHS at the product level with actual working hours or recommends a secondary approach with monetary data, when working hours are unavailable, to calculate the Occupational Health and Safety Potential (OHSP). The developed model was tested in a theoretical case study on shirt production in Europe and globally. The case study shows that the European shirt value chain resulted in higher OHSP values than the global shirt values chain. In addition, the model shows which life cycle stages and organizations highly contributed to the OHSP results. In both approaches, the shirt production stage contributed highly. Differences in results emerged based on the calculation approach, underscoring the model’s versatility, because increasing the complexity of calculating the CFs with monetary values will affect the results based on sectorial monetary output. Additionally, the study mentions benefits to the operationalization of social impact assessment and limitations when the developed characterized model is employed. Last, this study aids in offering a tool for organizations to meet the demands of the new Corporate Sustainability Reporting Directive by quantifying and publicizing OHS data. Full article
(This article belongs to the Section Development Goals towards Sustainability)
17 pages, 9099 KiB  
Article
The Assessment of Residents’ Perception of Possible Benefits and Challenges of Home Vertical Gardens in Kigali, Rwanda
by Rahman Tafahomi, David Nkurunziza, Gatoni Gwladys Benineza, Reihaneh Nadi and Regis Dusingizumuremyi
Sustainability 2024, 16(9), 3849; https://doi.org/10.3390/su16093849 (registering DOI) - 03 May 2024
Abstract
This paper aimed to provide a new insight into the application of home vertical gardens in Kigali, the capital of Rwanda, through a pre-assessment of the inhabitants’ perceptions. There are several studies that indicated the awareness of the way residents think about the [...] Read more.
This paper aimed to provide a new insight into the application of home vertical gardens in Kigali, the capital of Rwanda, through a pre-assessment of the inhabitants’ perceptions. There are several studies that indicated the awareness of the way residents think about the potential benefits and challenges of home gardens could make a considerable difference in designing and implementing these gardens. The Likert-scaled questionnaire (n = 558) was employed to evaluate how residents perceive vertical gardens, and what issues concern them most. The findings revealed that dwellers are almost familiar with the vertical garden concept and its possible effects on urban environments. The respondents mostly regarded vertical gardens as nice spots to socialize, relax, and interact with nature, and an opportunity for beautification, and recreation by growing ornamental and edible plants. However, they were rather apprehensive about some issues, more importantly, the extra expenses, the complicated operation and maintenance, and the type of structure installed on walls. In conclusion, small-scale and low-cost vertical gardens with lightweight structures and easy-to-use technologies are more likely to encourage householders to embrace home gardens. It is recommended that the vertical garden projects should be integrated into the urban green network strategy, leading to facilitating the processes of decision-making and financing. Full article
(This article belongs to the Section Sustainable Urban and Rural Development)
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24 pages, 4272 KiB  
Article
JPSSL: SAR Terrain Classification Based on Jigsaw Puzzles and FC-CRF
by Zhongle Ren, Yiming Lu, Biao Hou, Weibin Li and Feng Sha
Remote Sens. 2024, 16(9), 1635; https://doi.org/10.3390/rs16091635 (registering DOI) - 03 May 2024
Abstract
Effective features play an important role in synthetic aperture radar (SAR) image interpretation. However, since SAR images contain a variety of terrain types, it is not easy to extract effective features of different terrains from SAR images. Deep learning methods require a large [...] Read more.
Effective features play an important role in synthetic aperture radar (SAR) image interpretation. However, since SAR images contain a variety of terrain types, it is not easy to extract effective features of different terrains from SAR images. Deep learning methods require a large amount of labeled data, but the difficulty of SAR image annotation limits the performance of deep learning models. SAR images have inevitable geometric distortion and coherence speckle noise, which makes it difficult to extract effective features from SAR images. If effective semantic context features cannot be learned for SAR images, the extracted features struggle to distinguish different terrain categories. Some existing terrain classification methods are very limited and can only be applied to some specified SAR images. To solve these problems, a jigsaw puzzle self-supervised learning (JPSSL) framework is proposed. The framework comprises a jigsaw puzzle pretext task and a terrain classification downstream task. In the pretext task, the information in the SAR image is learned by completing the SAR image jigsaw puzzle to extract effective features. The terrain classification downstream task is trained using only a small number of labeled data. Finally, fully connected conditional random field processing is performed to eliminate noise points and obtain a high-quality terrain classification result. Experimental results on three large-scene high-resolution SAR images confirm the effectiveness and generalization of our method. Compared with the supervised methods, the features learned in JPSSL are highly discriminative, and the JPSSL achieves good classification accuracy when using only a small amount of labeled data. Full article
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32 pages, 21234 KiB  
Article
Anthropomorphic Tendon-Based Hands Controlled by Agonist–Antagonist Corticospinal Neural Network
by Francisco García-Córdova, Antonio Guerrero-González and Fernando Hidalgo-Castelo
Sensors 2024, 24(9), 2924; https://doi.org/10.3390/s24092924 (registering DOI) - 03 May 2024
Abstract
This article presents a study on the neurobiological control of voluntary movements for anthropomorphic robotic systems. A corticospinal neural network model has been developed to control joint trajectories in multi-fingered robotic hands. The proposed neural network simulates cortical and spinal areas, as well [...] Read more.
This article presents a study on the neurobiological control of voluntary movements for anthropomorphic robotic systems. A corticospinal neural network model has been developed to control joint trajectories in multi-fingered robotic hands. The proposed neural network simulates cortical and spinal areas, as well as the connectivity between them, during the execution of voluntary movements similar to those performed by humans or monkeys. Furthermore, this neural connection allows for the interpretation of functional roles in the motor areas of the brain. The proposed neural control system is tested on the fingers of a robotic hand, which is driven by agonist–antagonist tendons and actuators designed to accurately emulate complex muscular functionality. The experimental results show that the corticospinal controller produces key properties of biological movement control, such as bell-shaped asymmetric velocity profiles and the ability to compensate for disturbances. Movements are dynamically compensated for through sensory feedback. Based on the experimental results, it is concluded that the proposed biologically inspired adaptive neural control system is robust, reliable, and adaptable to robotic platforms with diverse biomechanics and degrees of freedom. The corticospinal network successfully integrates biological concepts with engineering control theory for the generation of functional movement. This research significantly contributes to improving our understanding of neuromotor control in both animals and humans, thus paving the way towards a new frontier in the field of neurobiological control of anthropomorphic robotic systems. Full article
(This article belongs to the Special Issue Tactile Sensors for Robotics Applications)
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12 pages, 1375 KiB  
Article
Mapping of Leaf Rust Resistance Loci in Two Kenyan Wheats and Development of Linked Markers
by Davinder Singh, Peace Kankwatsa, Karanjeet S. Sandhu, Urmil K. Bansal, Kerrie L. Forrest and Robert F. Park
Genes 2024, 15(5), 583; https://doi.org/10.3390/genes15050583 (registering DOI) - 03 May 2024
Abstract
Leaf rust caused by the pathogen Puccinia triticina (Pt) is a destructive fungal disease of wheat that occurs in almost all wheat-growing areas across the globe. Genetic resistance has proven to be the best solution to mitigate the disease. Wheat breeders [...] Read more.
Leaf rust caused by the pathogen Puccinia triticina (Pt) is a destructive fungal disease of wheat that occurs in almost all wheat-growing areas across the globe. Genetic resistance has proven to be the best solution to mitigate the disease. Wheat breeders are continuously seeking new diversified and durable sources of resistance to use in developing new varieties. We developed recombinant inbred line (RIL) populations from two leaf rust-resistant genotypes (Kenya Kudu and AUS12568) introduced from Kenya to identify and characterize resistance to Pt and to develop markers linked closely to the resistance that was found. Our studies detected four QTL conferring adult plant resistance (APR) to leaf rust. Two of these loci are associated with known genes, Lr46 and Lr68, residing on chromosomes 1B and 7B, respectively. The remaining two, QLrKK_2B and QLrAus12568_5A, contributed by Kenya Kudu and AUS12568 respectively, are putatively new loci for Pt resistance. Both QLrKK_2B and QLrAus12568_5A were found to interact additively with Lr46 in significantly reducing the disease severity at adult plant growth stages in the field. We further developed a suite of six closely linked markers within the QLrAus12568_5A locus and four within the QLrKK_2B region. Among these, markers sunKASP_522 and sunKASP_524, flanking QLrAus12568_5A, and sunKASP_536, distal to QLrKK_2B, were identified as the most closely linked and reliable for marker-assisted selection. The markers were validated on a selection of 64 Australian wheat varieties and found to be polymorphic and robust, allowing for clear allelic discrimination. The identified new loci and linked molecular markers will enable rapid adoption by breeders in developing wheat varieties carrying diversified and durable resistance to leaf rust. Full article
(This article belongs to the Collection Feature Papers: 'Plant Genetics and Genomics' Section)
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18 pages, 1473 KiB  
Article
Synthesis, Electronic, and Antibacterial Properties of 3,7-Di(hetero)aryl-substituted Phenothiazinyl N-Propyl Trimethylammonium Salts
by Hilla Khelwati, Lasse van Geelen, Rainer Kalscheuer and Thomas J. J. Müller
Molecules 2024, 29(9), 2126; https://doi.org/10.3390/molecules29092126 (registering DOI) - 03 May 2024
Abstract
In this study, a library of 3,7-di(hetero)aryl-substituted 10-(3-trimethylammoniumpropyl)10H-phenothiazine salts is prepared. These title compounds and their precursors are reversible redox systems with tunable potentials. The Hammett correlation gives a very good correlation of the first oxidation potentials with σp parameters. [...] Read more.
In this study, a library of 3,7-di(hetero)aryl-substituted 10-(3-trimethylammoniumpropyl)10H-phenothiazine salts is prepared. These title compounds and their precursors are reversible redox systems with tunable potentials. The Hammett correlation gives a very good correlation of the first oxidation potentials with σp parameters. Furthermore, the title compounds and their precursors are blue to green-blue emissive. Screening of the salts reveals for some derivatives a distinct inhibition of several pathogenic bacterial strains (Mycobacterium tuberculosis, Staphylococcus aureus, Escherichia coli, Aconetobacter baumannii, and Klebsiella pneumoniae) in the lower micromolar range. Full article
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15 pages, 2678 KiB  
Article
Oxidovanadium(V) Schiff Base Complexes Derived from Chiral 3-amino-1,2-propanediol Enantiomers: Synthesis, Spectroscopic Studies, Catalytic and Biological Activity
by Grzegorz Romanowski, Justyna Budka and Iwona Inkielewicz-Stepniak
Int. J. Mol. Sci. 2024, 25(9), 5010; https://doi.org/10.3390/ijms25095010 (registering DOI) - 03 May 2024
Abstract
Oxidovanadium(V) complexes, [(+)VOL1-5] and [(–)VOL1-5], with chiral tetradentate Schiff bases, which are products of monocondensation of S(‒)-3-amino-1,2-propanediol or R(+)-3-amino-1,2-propanediol with salicylaldehyde derivatives, have been synthesized. Different spectroscopic methods, viz. 1H and 51V NMR, IR, [...] Read more.
Oxidovanadium(V) complexes, [(+)VOL1-5] and [(–)VOL1-5], with chiral tetradentate Schiff bases, which are products of monocondensation of S(‒)-3-amino-1,2-propanediol or R(+)-3-amino-1,2-propanediol with salicylaldehyde derivatives, have been synthesized. Different spectroscopic methods, viz. 1H and 51V NMR, IR, UV-Vis, and circular dichroism, as well as elemental analysis, have been used for their detailed characterization. Furthermore, the epoxidation of styrene, cyclohexene, and two monoterpenes, S(‒)-limonene and (‒)-α-pinene, using two oxidants, aqueous 30% H2O2 or tert-butyl hydroperoxide (TBHP) in decane, has been studied with catalytic amounts of all complexes. Finally, biological cytotoxicity studies have also been performed with these oxidovanadium(V) compounds for comparison with cis-dioxidomolybdenum(VI) Schiff base complexes with the same chiral ligands, as well as to determine the cytoprotection against the oxidative damage caused by 30% H2O2 in the HT-22 hippocampal neuronal cells in the range of their 10–100 μM concentration. Full article
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11 pages, 5970 KiB  
Article
Preparation of Two-Dimensional Polyaniline Sheets with High Crystallinity via Surfactant Interface Self-Assembly and Their Encryption Application
by Zhiwei Li
Polymers 2024, 16(9), 1285; https://doi.org/10.3390/polym16091285 (registering DOI) - 03 May 2024
Abstract
In recent years in the field of traditional materials, traditional polyaniline has faced a number of scientific problems such as an irregular morphology, high difficulty in crystallization, and difficulty in forming an ordered structure compared to the corresponding inorganic materials. In response to [...] Read more.
In recent years in the field of traditional materials, traditional polyaniline has faced a number of scientific problems such as an irregular morphology, high difficulty in crystallization, and difficulty in forming an ordered structure compared to the corresponding inorganic materials. In response to these urgent issues, this study determines how to prepare a highly ordered structure in polyaniline formed at the gas-liquid interface. By dynamically arranging aniline monomers into a highly ordered structure with sodium dodecyl benzene sulfonate (SDBS) surfactant, aniline polymerization is initiated at the gas-liquid interface, resulting in two-dimensional polyaniline crystal sheets with a highly ordered structure. By elucidating the microstructure, crystallization process, and molecular structure of the two-dimensional polyaniline crystal sheets, the practical application of polyaniline as an encryption label in the field of electrochromism has been further expanded, thus making polyaniline widely used in the field of information encryption. Therefore, the synthesis of flaky polyaniline crystal sheets has a role in scientific research and practical application, which will arouse the interest and exploration of researchers. Full article
(This article belongs to the Section Polymer Chemistry)
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16 pages, 2537 KiB  
Article
Prospective Life Cycle Assessment of Biological Methanation in a Trickle-Bed Pilot Plant and a Potential Scale-Up
by Michael Heberl, Christian Withelm, Anja Kaul, Daniel Rank and Michael Sterner
Energies 2024, 17(9), 2206; https://doi.org/10.3390/en17092206 (registering DOI) - 03 May 2024
Abstract
The fluctuating nature of renewable energies results in the need for sustainable storage technologies to defossilize the energy system without other negative consequences for humans and the environment. In this study, a pilot-scale trickle-bed reactor for biological methanation and various scale-up scenarios for [...] Read more.
The fluctuating nature of renewable energies results in the need for sustainable storage technologies to defossilize the energy system without other negative consequences for humans and the environment. In this study, a pilot-scale trickle-bed reactor for biological methanation and various scale-up scenarios for 2024 and 2050 were investigated using life cycle assessment. A best- and worst-case scenario for technology development until 2050 was evolved using cross-consistency analysis and a morphological field, based on which the data for the ecological models were determined. The results show that the plant scale-up has a very positive effect on the ecological consequences of methanation. In the best-case scenario, the values are a factor of 23–780 lower than those of the actual plant today. A hot-spot analysis showed that electrolysis operation has an especially large impact on total emissions. The final Monte Carlo simulation shows that the technology is likely to achieve a low global warming potential with a median of 104.0 kg CO2-eq/MWh CH4 and thus can contribute to decarbonization. Full article
(This article belongs to the Topic Sustainable Energy Technology, 2nd Volume)
12 pages, 317 KiB  
Article
Scale Mixture of Gleser Distribution with an Application to Insurance Data
by Neveka M. Olmos, Emilio Gómez-Déniz and Osvaldo Venegas
Mathematics 2024, 12(9), 1397; https://doi.org/10.3390/math12091397 (registering DOI) - 03 May 2024
Abstract
In this paper, the scale mixture of the Gleser (SMG) distribution is introduced. This new distribution is the product of a scale mixture between the Gleser (G) distribution and the Beta(a,1) distribution. The SMG distribution is an alternative [...] Read more.
In this paper, the scale mixture of the Gleser (SMG) distribution is introduced. This new distribution is the product of a scale mixture between the Gleser (G) distribution and the Beta(a,1) distribution. The SMG distribution is an alternative to distributions with two parameters and a heavy right tail. We study its representation and some basic properties, maximum likelihood inference, and Fisher’s information matrix. We present an application to a real dataset in which the SMG distribution shows a better fit than two other known distributions. Full article
(This article belongs to the Special Issue Probabilistic Models in Insurance and Finance)
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25 pages, 19602 KiB  
Article
Real-Time EtherCAT-Based Control Architecture for Electro-Hydraulic Humanoid
by Maysoon Ghandour, Subhi Jleilaty, Naima Ait Oufroukh, Serban Olaru and Samer Alfayad
Mathematics 2024, 12(9), 1405; https://doi.org/10.3390/math12091405 (registering DOI) - 03 May 2024
Abstract
Electro-hydraulic actuators have witnessed significant development over recent years due to their remarkable abilities to perform complex and dynamic movements. Integrating such an actuator in humanoids is highly beneficial, leading to a humanoid capable of performing complex tasks requiring high force. This highlights [...] Read more.
Electro-hydraulic actuators have witnessed significant development over recent years due to their remarkable abilities to perform complex and dynamic movements. Integrating such an actuator in humanoids is highly beneficial, leading to a humanoid capable of performing complex tasks requiring high force. This highlights the importance of safety, especially since high power output and safe interaction seem to be contradictory; the greater the robot’s ability to generate high dynamic movements, the more difficult it is to achieve safety, as this requires managing a large amount of motor energy before, during, and after the collision. No matter what technology or algorithm is used to achieve safety, none can be implemented without a stable control system. Hence, one of the main parameters remains the quality and reliability of the robot’s control architecture through handling a huge amount of data without system failure. This paper addresses the development of a stable control architecture that ensures, in later stages, that the safety algorithm is implemented correctly. The optimum control architecture to utilize and ensure the maximum benefit of electro-hydraulic actuators in humanoid robots is one of the important subjects in this field. For a stable and safe functioning of the humanoid, the development of the control architecture and the communication between the different components should adhere to some requirements such as stability, robustness, speed, and reduced complexity, ensuring the easy addition of numerous components. This paper presents the developed control architecture for an underdeveloped electro-hydraulic actuated humanoid. The proposed solution has the advantage of being a distributed, real-time, open-source, modular, and adaptable control architecture, enabling simple integration of numerous sensors and actuators to emulate human actions and safely interact with them. The contribution of this paper is an enhancement of the updated rate compared to other humanoids by 20% and by 40 % in the latency of the master. The results demonstrate the potential of using EtherCAT fieldbus and open-source software to develop a stable robot control architecture capable of integrating safety and security algorithms in later stages. Full article
(This article belongs to the Special Issue Dynamic Modeling and Simulation for Control Systems, 2nd Edition)
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9 pages, 507 KiB  
Article
Degree of Hamstring Extensibility and Its Relationship with Pelvic Tilt in Professional Cyclists
by José M. Muyor, Pedro A. López-Miñarro, Fernando Alacid and Daniel López-Plaza
Appl. Sci. 2024, 14(9), 3912; https://doi.org/10.3390/app14093912 (registering DOI) - 03 May 2024
Abstract
The cyclist’s posture is typically characterized by a trunk flexion position to reach the handlebar of the bike. The pelvis serves as the base of the spine, and its tilt has been associated with the degree of extensibility of the hamstring, particularly in [...] Read more.
The cyclist’s posture is typically characterized by a trunk flexion position to reach the handlebar of the bike. The pelvis serves as the base of the spine, and its tilt has been associated with the degree of extensibility of the hamstring, particularly in flexion postures of the trunk. The aim of this study was to determine whether, in professional cyclists, the degree of hamstring extensibility influences the pelvic tilt maintained while seated on the bicycle with support from the three handlebar grips of the road bike, as well as in other positions of the bicycle. To evaluate pelvic tilt, all participants were measured using the Spinal Mouse system. The results revealed statistically significant differences in pelvic tilt among the six positions assessed (p ≤ 0.05). Furthermore, the degree of hamstring extensibility of the hamstrings presented a strong and positive correlation with pelvic tilt in standing posture (r = 0.82), Sit-and-Reach (r = 0.76), and Toe-Touch (r = 0.88). However, the degree of hamstring extensibility showed no significant correlations with pelvic tilt in any posture maintained on the bicycle. Full article
(This article belongs to the Special Issue Research of Sports Medicine on Health Care)
13 pages, 2526 KiB  
Article
Toe Box Shape of Running Shoes Affects In-Shoe Foot Displacement and Deformation: A Randomized Crossover Study
by Chengyuan Zhu, Yang Song, Yufan Xu, Aojie Zhu, Julien S. Baker, Wei Liu and Yaodong Gu
Bioengineering 2024, 11(5), 457; https://doi.org/10.3390/bioengineering11050457 (registering DOI) - 03 May 2024
Abstract
Background: Long-distance running is popular but associated with a high risk of injuries, particularly toe-related injuries. Limited research has focused on preventive measures, prompting exploration into the efficacy of raised toe box running shoes. Purpose: This study aimed to investigate the effect of [...] Read more.
Background: Long-distance running is popular but associated with a high risk of injuries, particularly toe-related injuries. Limited research has focused on preventive measures, prompting exploration into the efficacy of raised toe box running shoes. Purpose: This study aimed to investigate the effect of running shoes with raised toe boxes on preventing toe injuries caused by distance running. Methods: A randomized crossover design involved 25 male marathon runners (height: 1.70 ± 0.02 m, weight: 62.6 + 4.5 kg) wearing both raised toe box (extended by 8 mm along the vertical axis and 3 mm along the sagittal axis) and regular toe box running shoes. Ground reaction force (GRF), in-shoe displacement, and degree of toe deformation (based on the distance change between the toe and the metatarsal head) were collected. Results: Wearing raised toe box shoes resulted in a significant reduction in vertical (p = 0.001) and antero–posterior (p = 0.015) ground reaction forces during the loading phase, with a notable increase in vertical ground reaction force during the toe-off phase (p < 0.001). In-shoe displacement showed significant decreased movement in the forefoot medial (p < 0.001) and rearfoot (medial: p < 0.001, lateral: p < 0.001) and significant increased displacement in the midfoot (medial: p = 0.002, lateral: p < 0.001). Impact severity on the hallux significantly decreased (p < 0.001), while impact on the small toes showed no significant reduction (p = 0.067). Conclusions: Raised toe box running shoes offer an effective means of reducing toe injuries caused by long-distance running. Full article
(This article belongs to the Special Issue Multiscale Modeling in Computational Biomechanics)
12 pages, 4325 KiB  
Article
Theoretical Modeling and Mechanical Characterization at Increasing Temperatures under Compressive Loads of Al Core and Honeycomb Sandwich
by Alessandra Ceci, Girolamo Costanza and Maria Elisa Tata
Metals 2024, 14(5), 544; https://doi.org/10.3390/met14050544 (registering DOI) - 03 May 2024
Abstract
This work investigates the mechanical behavior under out-of-plane compression of the Al core and honeycomb sandwich at increasing temperatures of up to 300 °C. After the first introductive theoretical modeling on room-temperature compressive behavior, the experimental results at increasing temperatures up to 300 [...] Read more.
This work investigates the mechanical behavior under out-of-plane compression of the Al core and honeycomb sandwich at increasing temperatures of up to 300 °C. After the first introductive theoretical modeling on room-temperature compressive behavior, the experimental results at increasing temperatures up to 300 °C are presented and discussed. The analysis of the results shows that peak stress, plateau stress, and specific absorbed energy gradually decrease as the temperature increases. The final densification occurs always at the same strain level (around 75%). Sandwich honeycomb test temperatures have been limited to 200 °C for bonding problems of the skin to the sandwich due to the glue. The experimental and modeling results agree well at room temperature as well at increasing temperatures. The results can provide useful information to choose base materials for greater energy absorption at increasing temperatures. Full article
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11 pages, 286 KiB  
Article
Effect of Butorphanol-Medetomidine and Butorphanol-Dexmedetomidine on Echocardiographic Parameters during Propofol Anaesthesia in Dogs
by Andrej Bočkay, Carlos Fernando Agudelo, Mária Figurová, Nela Vargová and Alexandra Trbolová
Animals 2024, 14(9), 1379; https://doi.org/10.3390/ani14091379 (registering DOI) - 03 May 2024
Abstract
This study compared the effects of butorphanol-medetomidine and butorphanol-dexmedetomidine combinations on echocardiographic parameters during propofol anaesthesia in dogs. The dogs were randomly divided into two groups. In the butorphanol-medetomidine (BM) group, butorphanol (0.2 mg/kg) and medetomidine (15 μg/kg) were intravenously administered; in the [...] Read more.
This study compared the effects of butorphanol-medetomidine and butorphanol-dexmedetomidine combinations on echocardiographic parameters during propofol anaesthesia in dogs. The dogs were randomly divided into two groups. In the butorphanol-medetomidine (BM) group, butorphanol (0.2 mg/kg) and medetomidine (15 μg/kg) were intravenously administered; in the butorphanol-dexmedetomidine (BD) group, butorphanol (0.2 mg/kg) and dexmedetomidine (7.5 μg/kg) was used. Anaesthesia was induced with propofol and maintained with a constant-rate infusion of propofol (0.2 mg/kg/min). The echocardiographic parameters were assessed in conscious dogs (T0). Echocardiography was conducted again at 10 min post premedication (T1), followed by assessments at 30 (T2), 60 (T3), and 90 (T4) mins. The dogs were subjected to diagnostic procedures (radiography, computed tomography) under anaesthesia. A significant reduction in heart rate and cardiac output was noted in both groups at T1. There was no significant difference in the stroke volume between the BM and BD groups. The application of butorphanol-dexmedetomidine caused a significant increase in the left ventricular internal diameter in diastole and the diameter of the left atrium compared to that caused by butorphanol-medetomidine. This study documented that butorphanol-medetomidine and butorphanol-dexmedetomidine combinations caused similar reductions in heart rate and cardiac output in both groups. ‘New´ valvular regurgitation occurred following their administration. Full article
(This article belongs to the Special Issue Anaesthesia and Analgesia in Companion Animals)
18 pages, 5269 KiB  
Article
Combining Transcriptome- and Metabolome-Analyzed Differentially Expressed Genes and Differential Metabolites in Development Period of Caoyuanheimo-1 (Agaricus sp.) from Inner Mongolia, China
by Hai-yan Wang, Ya-nan Lu, Ya-jiao Li, Guo-qin Sun, Yuan Wu, Rui-qing Ji and Wei Yan
Horticulturae 2024, 10(5), 469; https://doi.org/10.3390/horticulturae10050469 (registering DOI) - 03 May 2024
Abstract
Caoyuanheimo-1 (Agaricus sp.) is a delectable mushroom native to Inner Mongolia, China, belonging to the Agaricus genus and valued for both its edible and medicinal properties. Although it has been cultivated to a certain extent, the molecular mechanisms regulating its development remain [...] Read more.
Caoyuanheimo-1 (Agaricus sp.) is a delectable mushroom native to Inner Mongolia, China, belonging to the Agaricus genus and valued for both its edible and medicinal properties. Although it has been cultivated to a certain extent, the molecular mechanisms regulating its development remain poorly understood. Building on our understanding of its growth and development conditions at various stages, we conducted transcriptomic and metabolomic studies to identify the differentially expressed genes (DEGs) and metabolites throughout its growth cycle. Simultaneously, we analyzed the synthesis pathways and identified several key genes involved in the production of terpenoids, which are secondary metabolites with medicinal value widely found in mushrooms. A total of 6843 unigenes were annotated, and 449 metabolites were detected in our study. Many of these metabolites and differentially expressed genes (DEGs) are involved in the synthesis and metabolism of amino acids, such as arginine, cysteine, methionine, and other amino acids, which indicates that the genes related to amino acid metabolism may play an important role in the fruiting body development of Caoyuanheimo-1. Succinic acid also showed a significant positive correlation with the transcriptional level changes of nine genes, including laccase-1 (TRINITY_DN5510_c0_g1), fruiting body protein SC3 (TRINITY_DN3577_c0_g1), and zinc-binding dihydrogenase (TRINITY_DN2099_c0_g1), etc. Additionally, seventeen terpenoids and terpenoid-related substances were identified, comprising five terpenoid glycosides, three monoterpenoids, two diterpenoids, one sesquiterpenoid, one sesterterpenoid, two terpenoid lactones, and three triterpenoids. The expression levels of the genes related to terpenoid synthesis varied across the three developmental stages. Full article
15 pages, 2439 KiB  
Article
Assessment of Anti-Prostate Cancer Activity among Four Seaweeds, with Focus on Caulerpa lentillifera J.Agardh
by Guan-James Wu and Pei-Wen Hsiao
Foods 2024, 13(9), 1411; https://doi.org/10.3390/foods13091411 (registering DOI) - 03 May 2024
Abstract
In response to a global shift towards health-conscious and environmentally sustainable food choices, seaweed has emerged as a focus for researchers due to its large-scale cultivation potential and the development of bioactive substances. This research explores the potential anticancer properties of seaweed extracts, [...] Read more.
In response to a global shift towards health-conscious and environmentally sustainable food choices, seaweed has emerged as a focus for researchers due to its large-scale cultivation potential and the development of bioactive substances. This research explores the potential anticancer properties of seaweed extracts, focusing on analyzing the impact of four common edible seaweeds in Taiwan on prostate cancer (PCa) cells’ activity. The study used bioassay-guided fractionation to extract Cl80 from various seaweeds with androgen receptor (AR)-inhibitory activity. Cl80 demonstrated effective suppression of 5α-dihydrotestosterone (DHT)-induced AR activity in 103E cells and attenuated the growth and prostate-specific antigen (PSA) protein expression in LNCaP and 22Rv1 cells. Additionally, Cl80 exhibited differential effects on various PCa cell lines. Concentrations above 5 μg/mL significantly inhibited LNCaP cell proliferation, while 22Rv1 cells were more resistant to Cl80. PC-3 cell proliferation was inhibited at 5 μg/mL but not completely at 50 μg/mL. A clonogenic assay showed that at a concentration of 0.5 μg/mL, the colony formation in LNCaP and PC-3 cells was significantly reduced, with a dose-dependent effect. Cl80 induced apoptosis in all PCa cell types, especially in LNCaP cells, with increased apoptotic cells observed at higher concentrations. Cl80 also decreased the mitochondrial membrane potential (ΔΨm) in a dose-dependent manner in all PCa cell lines. Furthermore, Cl80 suppressed the migration ability of PCa cells, with significant reductions observed in LNCaP, 22Rv1, and PC-3 cells at various concentrations. These compelling findings highlight the promising therapeutic potential of C. lentillifera J.Agardh and its isolated compound Cl80 in the treatment of PCa. Full article
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13 pages, 2028 KiB  
Article
Ionic Conductivity Analysis of NASICON Solid Electrolyte Coated with Polyvinyl-Based Polymers
by Tiago Afonso Salgueiro, Rita Carvalho Veloso, João Ventura, Federico Danzi and Joana Oliveira
Batteries 2024, 10(5), 157; https://doi.org/10.3390/batteries10050157 (registering DOI) - 03 May 2024
Abstract
The global environmental crisis necessitates reliable, sustainable, and safe energy storage solutions. The current systems are nearing their capacity limits due to the reliance on conventional liquid electrolytes, which are fraught with stability and safety concerns, prompting the exploration of solid-state electrolytes, which [...] Read more.
The global environmental crisis necessitates reliable, sustainable, and safe energy storage solutions. The current systems are nearing their capacity limits due to the reliance on conventional liquid electrolytes, which are fraught with stability and safety concerns, prompting the exploration of solid-state electrolytes, which enable the integration of metal electrodes. Solid-state sodium-ion batteries emerge as an appealing option by leveraging the abundance, low cost, and sustainability of sodium. However, low ionic conductivity and high interfacial resistance currently prevent their widespread adoption. This study explores polyvinyl-based polymers as wetting agents for the NASICON-type NZSP (Na3Zr2Si2PO12) solid electrolyte, resulting in a combined system with enhanced ionic conductivity suitable for Na-ion solid-state full cells. Electrochemical impedance spectroscopy (EIS) performed on symmetric cells employing NZSP paired with different wetting agent compositions demonstrates a significant reduction in interfacial resistance with the use of poly(vinyl acetate)—(PVAc-) based polymers, achieving an impressive ionic conductivity of 1.31 mS cm−1 at room temperature, 63.8% higher than the pristine material, notably reaching 7.36 mS cm−1 at 90 °C. These results offer valuable insights into the potential of PVAc-based polymers for advancing high-performance solid-state sodium-ion batteries by reducing their total internal resistance. Full article
(This article belongs to the Collection Advances in Battery Materials)
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19 pages, 9357 KiB  
Article
Quantification of Fundamental Textile Properties of Electronic Textiles Fabricated Using Different Techniques
by Arash M. Shahidi, Kalana Marasinghe, Parvin Ebrahimi, Jane Wood, Zahra Rahemtulla, Philippa Jobling, Carlos Oliveira, Tilak Dias and Theo Hughes-Riley
Textiles 2024, 4(2), 218-236; https://doi.org/10.3390/textiles4020013 (registering DOI) - 03 May 2024
Abstract
Electronic textiles (E-textiles) have experienced an increase in interest in recent years leading to a variety of new concepts emerging in the field. Despite these technical innovations, there is limited literature relating to the testing of E-textiles for some of the fundamental properties [...] Read more.
Electronic textiles (E-textiles) have experienced an increase in interest in recent years leading to a variety of new concepts emerging in the field. Despite these technical innovations, there is limited literature relating to the testing of E-textiles for some of the fundamental properties linked to wearer comfort. As such, this research investigates four fundamental properties of E-textiles: air permeability, drape, heat transfer, and moisture transfer. Three different types of E-textiles were explored: an embroidered electrode, a knitted electrode, and a knitted structure with an embedded electronic yarn. All of the E-textiles utilized the same base knitted fabric structure to facilitate a comparative study. The study used established textile testing practices to evaluate the E-textiles to ascertain the suitability of these standards for these materials. The study provides a useful point of reference to those working in the field and highlights some limitations of existing textile testing methodologies when applied to E-textiles. Full article
(This article belongs to the Special Issue Advances in Smart Textiles)
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8 pages, 1252 KiB  
Communication
SPAST Intragenic CNVs Lead to Hereditary Spastic Paraplegia via a Haploinsufficiency Mechanism
by Ewelina Elert-Dobkowska, Iwona Stepniak, Wiktoria Radziwonik-Fraczyk, Amir Jahic, Christian Beetz and Anna Sulek
Int. J. Mol. Sci. 2024, 25(9), 5008; https://doi.org/10.3390/ijms25095008 (registering DOI) - 03 May 2024
Abstract
The most common form of hereditary spastic paraplegia (HSP), SPG4 is caused by single nucleotide variants and microrearrangements in the SPAST gene. The high percentage of multi-exonic deletions or duplications observed in SPG4 patients is predisposed by the presence of a high frequency [...] Read more.
The most common form of hereditary spastic paraplegia (HSP), SPG4 is caused by single nucleotide variants and microrearrangements in the SPAST gene. The high percentage of multi-exonic deletions or duplications observed in SPG4 patients is predisposed by the presence of a high frequency of Alu sequences in the gene sequence. In the present study, we analyzed DNA and RNA samples collected from patients with different microrearrangements in SPAST to map gene breakpoints and evaluate the mutation mechanism. The study group consisted of 69 individuals, including 50 SPG4 patients and 19 healthy relatives from 18 families. Affected family members from 17 families carried varying ranges of microrearrangements in the SPAST gene, while one individual had a single nucleotide variant in the 5′UTR of SPAST. To detect the breakpoints of the SPAST gene, long-range PCR followed by sequencing was performed. The breakpoint sequence was detected for five different intragenic SPAST deletions and one duplication, revealing Alu-mediated microhomology at breakpoint junctions resulting from non-allelic homologous recombination in these patients. Furthermore, SPAST gene expression analysis was performed using patient RNA samples extracted from whole blood. Quantitative real-time PCR tests performed in 14 patients suggest no expression of transcripts with microrearrangements in 5 of them. The obtained data indicate that nonsense-mediated decay degradation is not the only mechanism of hereditary spastic paraplegia in patients with SPAST microrearrangements. Full article
(This article belongs to the Section Molecular Genetics and Genomics)
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13 pages, 1779 KiB  
Review
Flavonoid Oxidation Potentials and Antioxidant Activities-Theoretical Models Based on Oxidation Mechanisms and Related Changes in Electronic Structure
by Ante Miličević
Int. J. Mol. Sci. 2024, 25(9), 5011; https://doi.org/10.3390/ijms25095011 (registering DOI) - 03 May 2024
Abstract
Herein, I will review our efforts to develop a comprehensive and robust model for the estimation of the first oxidation potential, Ep1, and antioxidant activity, AA, of flavonoids that would, besides enabling fast and cheap prediction of Ep1 and AA [...] Read more.
Herein, I will review our efforts to develop a comprehensive and robust model for the estimation of the first oxidation potential, Ep1, and antioxidant activity, AA, of flavonoids that would, besides enabling fast and cheap prediction of Ep1 and AA for a flavonoid of interest, help us explain the relationship between Ep1, AA and electronic structure. The model development went forward with enlarging the set of flavonoids and, that way, we had to learn how to deal with the structural peculiarities of some of the 35 flavonoids from the final calibration set, for which the Ep1 measurements were all made in our laboratory. The developed models were simple quadratic models based either on atomic spin densities or differences in the atomic charges of the species involved in any of the three main oxidation mechanisms. The best model takes into account all three mechanisms of oxidation, single electron transfer-proton transfer (SET-PT), sequential proton loss electron transfer (SPLET) and hydrogen atom transfer (HAT), yielding excellent statistics (R2 = 0.970, S.E. = 0.043). Full article
(This article belongs to the Special Issue Molecular Simulation and Modeling)
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22 pages, 10574 KiB  
Article
Rats Orally Administered with Ethyl Alcohol for a Prolonged Time Show Histopathology of the Epididymis and Seminal Vesicle Together with Changes in the Luminal Metabolite Composition
by Chayakorn Taoto, Nareelak Tangsrisakda, Wipawee Thukhammee, Jutarop Phetcharaburanin, Sitthichai Iamsaard and Nongnuj Tanphaichitr
Biomedicines 2024, 12(5), 1010; https://doi.org/10.3390/biomedicines12051010 (registering DOI) - 03 May 2024
Abstract
Prolonged ethanol (EtOH) consumption is associated with male infertility, with a decreased spermatogenesis rate as one cause. The defective maturation and development of sperm during their storage in the cauda epididymis and transit in the seminal vesicle can be another cause, possibly occurring [...] Read more.
Prolonged ethanol (EtOH) consumption is associated with male infertility, with a decreased spermatogenesis rate as one cause. The defective maturation and development of sperm during their storage in the cauda epididymis and transit in the seminal vesicle can be another cause, possibly occurring before the drastic spermatogenesis disruption. Herein, we demonstrated that the cauda epididymis and seminal vesicle of rats, orally administered with EtOH under a regimen in which spermatogenesis was still ongoing, showed histological damage, including lesions, a decreased height of the epithelial cells and increased collagen fibers in the muscle layer, which implicated fibrosis. Lipid peroxidation (shown by malondialdehyde (MDA) levels) was observed, indicating that reactive oxygen species (ROS) were produced along with acetaldehyde during EtOH metabolism by CYP2E1. MDA, acetaldehyde and other lipid peroxidation products could further damage cellular components of the cauda epididymis and seminal vesicle, and this was supported by increased apoptosis (shown by a TUNEL assay and caspase 9/caspase 3 expression) in these two tissues of EtOH-treated rats. Consequently, the functionality of the cauda epididymis and seminal vesicle in EtOH-treated rats was impaired, as demonstrated by a decreases in 1H NMR-analyzed metabolites (e.g., carnitine, fructose), which were important for sperm development, metabolism and survival in their lumen. Full article
(This article belongs to the Special Issue Molecular Regulation of Spermatozoa)
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14 pages, 1596 KiB  
Article
An Analysis of the Main Nutrient Components of the Fruits of Different Macadamia (Macadamia integrifolia) Cultivars in Rocky Desertification Areas and a Comprehensive Evaluation of the Mineral Element Contents
by Zhuanmiao Kang, Guangzheng Guo, Fengping He, Hui Zeng, Xinghao Tu and Wenlin Wang
Horticulturae 2024, 10(5), 468; https://doi.org/10.3390/horticulturae10050468 (registering DOI) - 03 May 2024
Abstract
This study aims to understand the main nutrient composition and comprehensively evaluate the differences in the mineral element contents of fruits of different macadamia cultivars, as well as screen good cultivars that are suitable for use in rocky desert mountains. Nine macadamia nut [...] Read more.
This study aims to understand the main nutrient composition and comprehensively evaluate the differences in the mineral element contents of fruits of different macadamia cultivars, as well as screen good cultivars that are suitable for use in rocky desert mountains. Nine macadamia nut cultivars were selected as test materials in rocky desert mountain orchards. The contents of crude fat, crude protein, and total soluble sugar in kernels and N, P, K, Ca, Mg, Fe, Mn, Cu, Zn, and B in peels and kernels were determined, respectively. Then, the kernels’ mineral element contents were comprehensively evaluated based on principal component analysis. The results showed that the kernels were rich in crude fat, protein, and soluble sugar, with the crude fat content reaching 75% or greater, and the variation among cultivars was small. However, the variation in soluble sugar content was extensive. The content of mineral elements varied in different cultivars and parts of the fruit, with the average macronutrient content being K > N > Ca > P > Mg in the pericarp and N > K > P > Mg > Ca in the kernel, and the content of micronutrients in the pericarp and the kernel being Mn > Fe > Zn > Cu > B. By principal component analysis, the 10 mineral nutrient indexes were calculated as four principal components, with a cumulative contribution rate of 88.051%. Using the affiliation function value method and the calculation of the comprehensive evaluation value, the nine cultivars could be classified into three categories. The cultivar with the highest comprehensive evaluation value of the mineral element content was O.C. The one with the lowest value was H2, which indicated that O.C is a suitable variety for popularization in rocky desert mountainous areas. Stepwise regression analysis concluded that P, K, Fe, Mn, and Cu were the indicators significantly influencing the mineral element content of macadamia nuts and fruits in rocky desert mountains. Full article
(This article belongs to the Special Issue Fertilizer Usage and Nutrient Management in Horticultural Crops)
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