The duration of slow-wave sleep (SWS) is related to the reported sleep quality and to the important variables of mental and physical health. The internal cues to end an episode of SWS are poorly understood. One such internal cue is the initiation of a body movement, which is detectable as electromyographic (EMG) activity in sleep-electroencephalography (EEG). In the present study, we characterized the termination of SWS episodes by movement to explore its potential as a biomarker. To this end, we characterized the relation between the occurrence of SWS termination by movement and individual characteristics (age, sex), SWS duration and spectral content, chronotype, depression, medication, overnight memory performance, and, as a potential neurological application, epilepsy. We analyzed 94 full-night EEG-EMG recordings (75/94 had confirmed epilepsy) in the video-EEG monitoring unit of the EpiCARE Centre Salzburg, Austria. Segments of SWS were counted and rated for their termination by movement or not through the visual inspection of continuous EEG and EMG recordings. Multiple linear regression was used to predict the number of SWS episodes that ended with movement by depression, chronotype, type of epilepsy (focal, generalized, no epilepsy, unclear), medication, gender, total duration of SWS, occurrence of seizures during the night, occurrence of tonic-clonic seizures during the night, and SWS frequency spectra. Furthermore, we assessed whether SWS movement termination was related to overnight memory retention. According to multiple linear regression, patients with overall longer SWS experienced more SWS episodes that ended with movement (t = 5.64; p = 0.001). No other variable was related to the proportion of SWS that ended with movement, including no epilepsy-related variable. A small sample (n = 4) of patients taking Sertraline experienced no SWS that ended with movement, which was significant compared to all other patients (t = 8.00; p < 0.001) and to n = 35 patients who did not take any medication (t = 4.22; p < 0.001). While this result was based on a small subsample and must be interpreted with caution, it warrants replication in a larger sample with and without seizures to further elucidate the role of the movement termination of SWS and its potential to serve as a biomarker for sleep continuity and for medication effects on sleep. Full article

In the context of the increasing number of obese individuals, a major problem is represented by obesity and malnutrition in children. This condition is mainly ascribable to unbalanced diets characterized by high intakes of fat and sugar. Childhood obesity and malnutrition are not only associated with concurrent pathologies but potentially compromise adult life. Considering the strict correlation among systemic metabolism, obesity, and skeletal muscle health, we wanted to study the impact of juvenile malnutrition on the adult skeletal muscle. To this aim, 3-week-old C56BL/6 female and male mice were fed for 20 weeks on a high-fat. high-sugar diet, and their muscles were subjected to a histological evaluation. MyHCs expression, glycogen content, intramyocellular lipids, mitochondrial activity, and capillary density were analyzed on serial sections to obtain the metabolic profile. Our observations indicate that a high-fat, high-sugar diet alters the metabolic profile of skeletal muscles in a sex-dependent way and induces the increase in type II fibers, mitochondrial activity, and lipid content in males, while reducing the capillary density in females. These data highlight the sex-dependent response to nutrition, calling for the development of specific strategies and for a systematic inclusion of female subjects in basic and applied research in this field. Full article

 The valorization technique successfully transformed waste polyethylene terephthalate (PET) into valuable carbon nanomaterial (CN)/graphene, while doped and undoped ZnO nanopowders were synthesized via sol–gel methods. Utilizing XRD, BET, TEM, EDX, FTIR, and TGA analyses, the synthesis of sp² 2D sheet, pristine, and doped ZnO nanostructures was confirmed. Solid-state gas sensor devices, tested under 51% relative humidity (RH), 30 °C ambient temperature, and 0.2 flow rate, exhibited a 3.4% enhanced response to H₂ gas compared to CO₂ at 50 ppm concentrations over time. Notably, the ZnO/CN sensor surpassed CN and ZnO alone, attributed to CN dopant integration with decreasing order of response performance as ZnO/CN > CN > ZnO. This study underscores the efficacy of valorization techniques in generating high-value carbon nanomaterials and their efficacy in bolstering gas sensor performance, with ZnO/CN demonstrating superior response capabilities. Full article

Background: Severe and rigid scoliosis represents a type of spinal deformity characterized by a Cobb angle exceeding 90° and a flexibility of less than 30%. Halo spinal traction remains the established standard for managing severe scoliosis, although alternative approaches such as temporary internal distraction rods and staged surgical correction exist. The primary objective of this investigation was to compare two cohorts of patients treated using these distinct methods to ascertain any divergences in terms of surgical and radiological outcomes, pulmonary function (PF), and quality of life (QoL). Methods: This study encompassed a total of 62 pediatric patients meeting the specified criteria, which included severe idiopathic scoliosis (major Cobb curve >90) and flexibility <30%. Group 1 (G1) underwent surgical intervention involving preoperative Halo gravity traction (HGT) succeeded by posterior spinal fusion (PSF). On the other hand, Group 2 (G2) underwent a two-stage procedure starting with a less invasive temporary internal distraction technique (LITID) prior to PSF. The radiological outcomes, PF, and QoL were documented and assessed over a monitoring period ranging from 2 to 5 years. Results: The average preoperative major curves (MCs) measured 124° and 122° in G1 and G2, respectively (p < 0.426). Initial flexibility, as observed in preoperative bending films, ranged from 18% in G1 to 21% in G2 (p < 0.001). Following the ultimate surgical intervention, the MCs were corrected to 45° and 37.4° in G1 and G2, respectively (p < 0.001). The percentage correction of the MCs was higher in G2 (63% vs. 70% in G1 and G2, respectively), with significant between-group disparities (p < 0.001). The mean preoperative thoracic kyphoses (TKs) were 96.5° in G1 and 92° in G2 (p = 0.782), which were rectified to 45.8° in G1 and 36.2° in G2 (p < 0.001), equating to correction rates of 55% and 60% in the respective groups. Initially, G2 exhibited lower values for the percentage of predicted lung volume (FVC) and predicted FEV1 compared with G1 (49% and 58% vs. 54.5% and 60.8%; N.S.). Nonetheless, both groups demonstrated enhancements in their FVC and FEV1 values over the follow-up period. Conclusions: The surgical management of severe and untreated spinal curvatures in the pediatric and adolescent population can be considered safe, with a tolerable incidence of minor complications. LITID emerges as a method offering improved QoL and pulmonary function, achieving notably substantial average corrections in deformity by 70% in the coronal plane and 60% in the sagittal plane, alongside a mean increase in trunk height of 10.8 cm. Furthermore, a typical reduction of 76% in rib humps and enhancements in respiratory function, as indicated by improvements in 1 s predicted forced expiratory volume (by 25–56%) and forced vital capacity (by 35–65%), were achieved, leading to a clinically and statistically significant enhancement in QoL when evaluated using SRS-22r, without resorting to more radical, high-risk procedures. Full article

The aviation intelligent pump system is an effective solution to aircraft hydraulic systems’ inefficient power consumption and temperature increase. A self-supplied aviation intelligent pump (SAIP) has a high power-to-weight ratio and compact structure, making it the optimal choice for an intelligent pump. To analyze the output characteristics of a novel aviation intelligent pump, it is crucial to establish an accurate mathematical model that describes its dynamic characteristics. This can be achieved by analyzing the working principle and exploring the influence of critical parameters. The paper introduces the composition and working principle of a self-supplied electro-hydraulic servo variable displacement pump. It then establishes a mathematical model of the whole pump, with a detailed analysis and modeling of the critical variable mechanism and the swash plate assembly’s load moment. A simulation model was created to examine the impact of crucial structural parameters, such as the offset spring’s stiffness and control piston’s diameter, on the output characteristics of the intelligent pump. An experimental platform was also constructed, and the experimental results confirm the accuracy of the SAIP model presented in this paper. The investigation of the output characteristics fully reveals the dynamic performance of the SAIP. This provides the basis for the subsequent design of high-performance flow and pressure control strategies and aids in researching intelligent aircraft hydraulic systems. Full article

The Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, has a wide host range, extending from pigs and ardeid birds to opportunistic dead-end hosts, such as humans and horses. However, JEV encephalitis infections in aquatic mammals are rare, with only two cases in seals reported to date. Here, we report a lethal case of JEV and Schizophyllum commune co-infection in an aquarium-housed harbor seal in Japan. We isolated JEV from the brain of the dead seal and characterized its phylogeny and pathogenicity in mice. The virus isolate from the seal was classified as genotype GIb, which aligns with recent Japanese human and mosquito isolates as well as other seal viruses detected in China and Korea, and does not exhibit a unique sequence trait distinct from that of human and mosquito strains. We demonstrated that the seal isolate is pathogenic to mice and causes neuronal symptoms. These data suggest that seals should be considered a susceptible dead-end host for circulating JEV in natural settings. Full article

Eye tracking plays a crucial role in consumer research. The aim of this work is to present the statuses of studies that used eye tracking as an instrument in consumer research to investigate food from a marketing perspective. For this purpose, a bibliometric review of 118 articles from the Business Source Premier and Web of Science Core Collection database was compiled. The bibliometric review provides information on publication trends, leading authors, collaborative networks, journals, institutions, countries, articles, keywords, and themes investigated. Publications in the research field have appeared since 2011, primarily in Europe, the United States, and Uruguay. Three areas of research streams were identified: (1) how consumers became aware of and chose food, (2) nutritional information and its impact, and (3) how food information and its visual attention led to certain consumer behavior. The bibliographic review summarized past research directions and, thus, identified possibilities for future research streams. Full article

In smart education, adaptive e-learning systems personalize the educational process by tailoring it to individual learning styles. Traditionally, identifying these styles relies on learners completing surveys and questionnaires, which can be tedious and may not reflect their true preferences. Additionally, this approach assumes that learning styles are fixed, leading to a cold-start problem when automatically identifying styles based on e-learning platform behaviors. To address these challenges, we propose a novel approach that annotates unlabeled student feedback using multi-layer topic modeling and implements the Felder–Silverman Learning Style Model (FSLSM) to identify learning styles automatically. Our method involves learners answering four FSLSM-based questions upon logging into the e-learning platform and providing personal information like age, gender, and cognitive characteristics, which are weighted using fuzzy logic. We then analyze learners’ behaviors and activities using web usage mining techniques, classifying their learning sequences into specific styles with an advanced deep learning model. Additionally, we analyze textual feedback using latent Dirichlet allocation (LDA) for sentiment analysis to enhance the learning experience further. The experimental results demonstrate that our approach outperforms existing models in accurately detecting learning styles and improves the overall quality of personalized content delivery. Full article

Breast cancer is associated with high mortality and morbidity rates. As about 20–30% of patients exhibiting ER-positive phenotype are resistant to hormonal treatment with the standard drug tamoxifen, finding new therapies is a necessity. Postbiotics, metabolites, and macromolecules isolated from probiotic bacteria cultures have been proven to have sufficient bioactivity to exert prohealth and anticancer effects, making them viable adjunctive agents for the treatment of various neoplasms, including breast cancer. In the current study, postbiotics derived from L. plantarum and L. rhamnosus cultures were assessed on an in vitro breast cancer model as potential adjunctive agents to therapy utilizing tamoxifen and a candidate aziridine–hydrazide hydrazone derivative drug. Cell viability and cell death processes, including apoptosis, were analyzed for neoplastic MCF-7 cells treated with postbiotics and synthetic compounds. Cell cycle progression and proliferation were analyzed by PI-based flow cytometry and Ki-67 immunostaining. Postbiotics decreased viability and triggered apoptosis in MCF-7, modestly affecting the cell cycle and showing a lack of negative impact on normal cell viability. Moreover, they enhanced the cytotoxic effect of tamoxifen and the new candidate drug toward MCF-7, accelerating apoptosis and the inhibition of proliferation. This illustrates postbiotics’ potential as natural adjunctive agents supporting anticancer therapy based on synthetic drugs. Full article

IR-783, a commercially available near-infrared (NIR) heptamethine cyanine dye, has been used for selective tumor imaging in breast, prostate, cervical, and brain cancers in vitro and in vivo. Although the molecular mechanism behind the structure-inherent tumor targeting of IR-783 has not been well-demonstrated, IR-783 has unique properties such as a good water solubility and low cytotoxicity compared with other commercial heptamethine cyanine dyes. The goal of this study is to evaluate the phototherapeutic efficacy of IR-783 as a tumor-targeted photothermal agent in human colorectal cancer xenografts. The results demonstrate that IR-783 shows both the subcellular localization in HT-29 cancer cells and preferential accumulation in HT-29 xenografted tumors 24 h after its intravenous administration. Furthermore, the IR-783 dye reveals the superior capability to convert NIR light into heat energy under 808 nm NIR laser irradiation in vitro and in vivo, thereby inducing cancer cell death. Taken together, these findings suggest that water-soluble anionic IR-783 can be used as a bifunctional phototherapeutic agent for the targeted imaging and photothermal therapy (PTT) of colorectal cancer. Therefore, this work provides a simple and effective approach to develop biocompatible, hydrophilic, and tumor-targetable PTT agents for targeted cancer phototherapy. Full article

Protein meals from insects in combination with poultry by-product meal appear to be promising ingredients for replacing conventional proteins in the diets of carnivorous fish. The present study explored the effects on growth performance, hepatic enzymatic activity, and fillet physical and nutritional characteristics during a 66-day feeding trial performed on European seabass. A total of 3000 fish were distributed into three tanks, where the control group was fed with a commercial diet (CG) and a second group was fed in duplicate with the experimental diet (SSH) containing 10% Hermetia illucens larva meal, 30% poultry by-product meal, and < 5.5 g/100 g of feed of marine origin proteins. All fish showed good growth performance. Glucose-6-phosphate dehydrogenase, aspartate aminotransferase, and 3-hydroxyacyl-CoA dehydrogenase activities were higher in the SSH group than in the CG group. The fillet fatty acid profile was largely unaffected by diet, except for a few fatty acids. Fish fed the SSH diet had a lower C22:1n-11 content than CG, thus suggesting an increased β-oxidation. The oxidative status of muscle lipids was not affected by the diet. In conclusion, the present study showed that European seabass can be successfully fed the SSH diet for two months in a commercial setting. Full article

Neuroblastoma is a peripheral nervous system tumor that almost exclusively occurs in young children. Although intensified treatment modalities have led to increased patient survival, the prognosis for patients with high-risk disease is still around 50%, signifying neuroblastoma as a leading cause of cancer-related deaths in children. Neuroblastoma is an embryonal tumor and is shaped by its origin from cells within the neural crest. Hence, neuroblastoma usually presents with a low mutational burden and is, in the majority of cases, driven by epigenetically deregulated transcription networks. The recent development of Omic techniques has given us detailed knowledge of neuroblastoma evolution, heterogeneity, and plasticity, as well as intra- and intercellular molecular communication networks within the neuroblastoma microenvironment. Here, we discuss the potential of these recent discoveries with emphasis on new treatment modalities, including immunotherapies which hold promise for better future treatment regimens. Full article

A seven-year-old healthy female Chow Chow was referred for pregnancy monitoring. Ultrasonography was used to evaluate all pregnancy and fetus parameters, and they were found to be normal. During the examination of the 42 day pregnant bitch, an unusual mass was seen in a fetus’s heart. This fetus had a cardiac frequency of 273–300 beats, while the others had heart rates of 220–240 beats. Natural vaginal birth occurred at 63 days pregnant: the first two puppies were stillborn but perfectly formed, and the other three were alive and had optimal APGAR. In one of two deceased puppies, an unusual, reddish, smooth mass occupying the space in the heart was found through necroscopy. The organ was submitted for histological examination. Histopathology, immunohistochemical, and histochemical analyses all indicated a cardiac tumor with increased Purkinje cells. This type of tumor has been described in infants, swine, bearded seals, and deer but never in fetuses and neonates of dogs. To our knowledge, this is the first such case reported in veterinary medicine. Full article

The aim of the study was to evaluate whether the static stabilometric parameters among elite fencers, were affected by prolonged, asymmetric training regimen. A sample of 26 elite fencers of both genders, aged 19.15 ± 2.24 years, practising one of the three disciplines foil, épée, or sabre, was recruited for the study. Anthropometric measurements including thigh and calf circumferences and postural assessment based on the weight distribution on a stabilimeter platform were performed. Postural tone, as indicated by measures such as sway length and sway area ratio was calculated.. No notable anthropometric asymmetries were detected within the examined group The weight distribution patterns on the support quadrants in static stabilometric measurements did not suggest clinically significant issues. There were no significant differences among subgroups based on gender and lower limb dominance for both anthropometric and stabilimeter variables. However, 30.8% of participants showed anomalies in postural tone (hypertonic and hypotonic condition). Five out of eight athletes found with abnormal postural tone were foil fencers, suggesting a potential discipline-specific effect. Individual adjustments were found in foil fencers. These findings provide insights into the potential effects of fencing training on postural parameters among elite athletes. Full article

Nanling Mountain region is a typical southern hilly region, which plays an important ecological and environmental protection role in China’s overall land protection pattern. Based on the remote sensing image data of Longnan City in Nanling Mountain region in 2013, 2018 and 2023, this paper interpreted the land use type and analyzed the land use transfer situation by using land use transfer flow, and a land use transfer matrix. At the same time, based on the remote sensing ecological index (RSEI) model, the ecological environmental quality of Longnan City from 2013 to 2023 was retrieved. The temporal and spatial response model of the ecological environmental quality to land use transfer in Longnan City from 2013 to 2023 was discussed based on spatial autocorrelation and a geographical detector. The results show that from 2013 to 2023, the decrease of forest land (16.23 km²) and the increase of construction land (13.25 km²) were the main land use transfers in Longnan City. The ecological environment indexes of Longnan City in 2013, 2018 and 2023 were 0.789, 0.917 and 0.872, respectively, showing a trend of “first rising and then decreasing”. The ecological environmental quality in the north of Longnan City was significantly lower than that in the south, and the poor ecological quality area appeared in and around the northern main urban area, showing a trend of “inward contraction”. Forest land, garden land, grassland, cultivated land and water area have a positive impact on ecological environmental quality, while traffic land, construction land and other land have a negative impact on ecological environmental quality. The response of ecological environmental quality to different land use transfer modes is related to the change of the overall ecological environmental quality. The interaction between land use and land cover change (LUCC) and other factors had a great impact on the evolution of ecological environmental quality in Longnan City. Full article

Volatile organic compounds (VOCs) might be associated with pulmonary oxygen toxicity (POT). This pilot study aims to identify VOCs linked to oxidative stress employing an in vitro model of alveolar basal epithelial cells exposed to hyperbaric and hyperoxic conditions. In addition, the feasibility of this in vitro model for POT biomarker research was evaluated. The hyperbaric exposure protocol, similar to the U.S. Navy Treatment Table 6, was conducted on human alveolar basal epithelial cells, and the headspace VOCs were analyzed using gas chromatography–mass spectrometry. Three compounds (nonane [p = 0.005], octanal [p = 0.009], and decane [p = 0.018]), of which nonane and decane were also identified in a previous in vivo study with similar hyperbaric exposure, varied significantly between the intervention group which was exposed to 100% oxygen and the control group which was exposed to compressed air. VOC signal intensities were lower in the intervention group, but cellular stress markers (IL8 and LDH) confirmed increased stress and injury in the intervention group. Despite the observed reductions in compound expression, the model holds promise for POT biomarker exploration, emphasizing the need for further investigation into the complex relationship between VOCs and oxidative stress. Full article

This paper explores the potential of a new source of fly ash, deposited on the site of a coal-fired power plant in Ireland dating from 1985 to 1995, as a cement replacement material. A series of X-ray diffraction (XRD) analyses on binder samples with cement replacement levels of 0, 10, 25 and 35% was undertaken to determine the fly ash’s mineralogical composition and to determine its suitability as a supplemental cementitious material (SCM). The XRD results reveal a unique mineral composition with promising characteristics for enhancing the strength and durability of concrete. The experimental results were used to calibrate a thermodynamic model to predict changing phase assemblage and hydration behaviour over time and per replacement level. Thermodynamic models have been shown to give credible predictions of the long-term performance of cements, including SCMs. The initial experimental results’ thermodynamic modelling demonstrates the feasibility of this fly ash source as a sustainable alternative to traditional cement, paving the way for more eco-friendly construction. Ash deposits dating from 1995 to 2005 and from 2005 to the present will be presented in subsequent publications. Full article

A novel indolium-based fluorescent probe for the detection of CN⁻ was developed based on the conjugation of 1, 2, 3, 3-Tetramethyl-3H-indolium iodide and 2-acetyl benzothiophene. The introduction of external CN⁻ caused a nucleophilic attack to the quaternary amine salt structure in the probe and resulted in the departure of iodide ions and the steric rotation of the index salt group, which caused fluorescence quenching. The titration experiments showed that the probe had rapid qualitative and quantitative analysis capabilities for CN⁻. Moreover, the relevant biocompatibility experiments also demonstrated the potential application value of the probe. Full article

This article focuses on developing models that estimate suspended sediment concentrations (SSCs) for the Lower Brazos River, Texas, U.S. Historical samples of SSCs from gauge stations and satellite imagery from Landsat Missions and Sentinel Mission 2 were utilized to develop models to estimate SSCs for the Lower Brazos River. The models used in this study to accomplish this goal include support vector machines (SVMs), artificial neural networks (ANNs), extreme learning machines (ELMs), and exponential relationships. In addition, flow measurements were used to develop rating curves to estimate SSCs for the Brazos River as a baseline comparison of the models that used satellite imagery to estimate SSCs. The models were evaluated using a Taylor Diagram analysis on the test data set developed for the Brazos River data. Fifteen of the models developed using satellite imagery as inputs performed with a coefficient of determination R² above 0.69, with the three best performing models having an R² of 0.83 to 0.85. One of the best performing models was then utilized to estimate the SSCs before, during, and after Hurricane Harvey to evaluate the impact of this storm on the sediment dynamics along the Lower Brazos River and the model’s ability to estimate SSCs. Full article

Artificial reefs are man-made structures submerged in the ocean, and the design of these structures plays a crucial role in determining their effectiveness. Precisely measuring the configuration of artificial reefs is vital for creating suitable habitats for marine organisms. This study presents a novel approach for automated detection of artificial reefs by recognizing their key features and key points. Two enhanced models, namely, YOLOv8n-PoseRFSA and YOLOv8n-PoseMSA, are introduced based on the YOLOv8n-Pose architecture. The YOLOv8n-PoseRFSA model exhibits a 2.3% increase in accuracy in pinpointing target key points compared to the baseline YOLOv8n-Pose model, showcasing notable enhancements in recall rate, mean average precision (mAP), and other evaluation metrics. In response to the demand for swift identification in mobile fishing scenarios, a YOLOv8n-PoseMSA model is proposed, leveraging MobileNetV3 to replace the backbone network structure. This model reduces the computational burden to 33% of the original model while preserving recognition accuracy and minimizing the accuracy drop. The methodology outlined in this research enables real-time monitoring of artificial reef deployments, allowing for the precise quantification of their structural characteristics, thereby significantly enhancing monitoring efficiency and convenience. By better assessing the layout of artificial reefs and their ecological impact, this approach offers valuable data support for the future planning and implementation of reef projects. Full article

The rotational speed standard device that can carry loads is the key device for calibrating passive rotational speed sensors. The rotor of the passive rotational speed sensor is connected to the rotor of the standard speed device through a coupling, and the standard reference speed is provided by the standard device. Due to the rotor eccentricity, the unbalanced force of the rotor occurs, and it can not only affect the rotational speed accuracy but can also damage the mechanical bearings of the standard speed device. To solve this issue, a method for suppressing the unbalanced force of the speed standard device based on an active magnetic bearing (AMB) force compensation system is proposed. First, the overall structure of the system is briefly introduced. Then, the force feedback control system model with the AMB as the force actuator is established, and a PI controller is designed to achieve the disturbed force control. Finally, a semi-physical simulation experimental platform is built to verify the effectiveness of the proposed method. The experimental results show that the AMB force compensation system can reduce 84.4%, 81.6%, and 79.8% of the unbalanced vibration force at the frequency of 30 Hz, 90 Hz, and 150 Hz, respectively. Full article