The 2023 MDPI Annual Report has
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25 pages, 2365 KiB  
Article
Research on Multi-Stage Post-Occupancy Evaluation Framework of Community Comprehensive Elderly Care Service Facilities under the Public-Private Partnership Mode—A Case Study of China
by Lijun Lin, Lin Zhang, Shuai Geng, Yulin Zhao and Yuanyuan Tian
Buildings 2024, 14(5), 1343; https://doi.org/10.3390/buildings14051343 (registering DOI) - 9 May 2024
Abstract
The key to whether elderly individuals in the community can enjoy their later years peacefully lies in the service capabilities of community comprehensive elderly care service facilities (CCECSF) under the Public-Private Partnership (PPP) mode. To maintain a high level of service capability in [...] Read more.
The key to whether elderly individuals in the community can enjoy their later years peacefully lies in the service capabilities of community comprehensive elderly care service facilities (CCECSF) under the Public-Private Partnership (PPP) mode. To maintain a high level of service capability in community comprehensive elderly care service facilities under PPP mode, scientific evaluation of the effectiveness of these facilities is equally crucial. This article first constructs a post-occupancy evaluation index system of community comprehensive elderly care service facilities under PPP mode that includes three evaluation attributes and fifteen evaluation criteria based on the Chinese culture and lifestyle habits. Regarding the issue of direct users being unable to directly participate in evaluations, the uncertainty in evaluation information, and the volatility of evaluation results, a multi-stage post-occupancy evaluation model is constructed based on probabilistic linguistic term set, TOPSIS model and multi-stage decision theory. The above post-occupancy evaluation index system and evaluation model together constitute a multi-stage post-occupancy evaluation framework for community comprehensive elderly care service facilities under PPP mode. The outcomes of the case study indicate that the post-occupancy evaluation index system can offer a scientifically guided approach for evaluating the service level of community comprehensive elderly care service facilities under the PPP mode; meanwhile, the multi-stage evaluation model can enable direct user participation in the post-evaluation of facility usage and improve the robustness and reduce the fluctuation of the evaluation results, so as to improve the scientificity of the evaluation results. Full article
(This article belongs to the Section Architectural Design, Urban Science, and Real Estate)
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12 pages, 21037 KiB  
Article
Grooving and Absorption on Substrates to Reduce the Bulk Acoustic Wave for Surface Acoustic Wave Micro-Force Sensors
by Yang Feng, Haoda Yu, Wenbo Liu, Keyong Hu, Shuifa Sun, Zhen Yang and Ben Wang
Micromachines 2024, 15(5), 637; https://doi.org/10.3390/mi15050637 (registering DOI) - 9 May 2024
Abstract
Improving measurement accuracy is the core issue with surface acoustic wave (SAW) micro-force sensors. An electrode transducer can stimulate not only the SAW but also the bulk acoustic wave (BAW). A portion of the BAW can be picked up by the receiving transducer, [...] Read more.
Improving measurement accuracy is the core issue with surface acoustic wave (SAW) micro-force sensors. An electrode transducer can stimulate not only the SAW but also the bulk acoustic wave (BAW). A portion of the BAW can be picked up by the receiving transducer, leading to an unwanted or spurious signal. This can harm the device’s frequency response characteristics, thereby potentially reducing the precision of the micro-force sensor’s measurements. This paper examines the influence of anisotropy on wave propagation, and it also performs a phase-matching analysis between interdigital transducers (IDTs) and bulk waves. Two solutions are shown to reduce the influence of BAW for SAW micro sensors, which are arranged with acoustic absorbers at the ends of the substrate and in grooving in the piezoelectric substrate. Three different types of sensors were manufactured, and the test results showed that the sidelobes of the SAW micro-force sensor could be effectively inhibited (3.32 dB), thereby enhancing the sensitivity and performance of sensor detection. The SAW micro-force sensor manufactured using the new process was tested and the following results were obtained: the center frequency was 59.83 MHz, the fractional bandwidth was 1.33%, the range was 0–1000 mN, the linearity was 1.02%, the hysteresis was 0.59%, the repeatability was 1.11%, and the accuracy was 1.34%. Full article
(This article belongs to the Special Issue Recent Advances in SAW Resonators)
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23 pages, 1877 KiB  
Review
Blockchain for Organ Transplantation: A Survey
by Elif Calik and Malika Bendechache
Blockchains 2024, 2(2), 150-172; https://doi.org/10.3390/blockchains2020008 (registering DOI) - 9 May 2024
Abstract
As blockchain becomes more widely used, a growing number of application fields are becoming interested in blockchain to benefit from its decentralised nature, invariability, security, transparency, quick transaction capabilities, and cost-effectiveness. Blockchain has a wide range of applications and uses in healthcare. Distributed [...] Read more.
As blockchain becomes more widely used, a growing number of application fields are becoming interested in blockchain to benefit from its decentralised nature, invariability, security, transparency, quick transaction capabilities, and cost-effectiveness. Blockchain has a wide range of applications and uses in healthcare. Distributed ledger technology facilitates the secure transfer of patient medical records, manages the medicine supply chain, and creates an efficient, transparent, safe, and effective way of communicating data across global healthcare. The organ transplantation process (OTP) is one of the healthcare areas that benefit from the use of such technology to make its process more secure and transparent. In this article, we put forward a systematic literature review analysis on the application of blockchain to the OTP. Additionally, we address and highlight the barriers and challenges that arise while using blockchain technology for the OTP. We also offer some suggestions for future developments that would enhance blockchain’s implementation in the OTP domain. Full article
(This article belongs to the Special Issue Feature Papers in Blockchains)
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25 pages, 3873 KiB  
Article
Sliding Mode Speed Control in Synchronous Motors for Agriculture Machinery: A Chattering Suppression Approach
by David Marcos-Andrade, Francisco Beltran-Carbajal, Ivan Rivas-Cambero, Hugo Yañez-Badillo, Antonio Favela-Contreras and Julio C. Rosas-Caro
Agriculture 2024, 14(5), 737; https://doi.org/10.3390/agriculture14050737 (registering DOI) - 9 May 2024
Abstract
Synchronous motors have extended their presence in different applications, specifically in high-demand environments such as agronomy. These uses need advanced and better control strategies to improve energy efficiency. Within this context, sliding mode control has demonstrated effectiveness in electric machine control due to [...] Read more.
Synchronous motors have extended their presence in different applications, specifically in high-demand environments such as agronomy. These uses need advanced and better control strategies to improve energy efficiency. Within this context, sliding mode control has demonstrated effectiveness in electric machine control due to its advantages in robustness and quick adaptation to uncertain dynamic system disturbances. Nevertheless, this control technique presents the undesirable chattering phenomenon due to the discontinuous control action. This paper introduces a novel speed integral control scheme based on sliding modes for synchronous motors. This approach is designed to track smooth speed profiles and is evaluated through several numeric simulations to verify its robustness against variable torque loads. This approach addresses using electric motors for different applications such as irrigation systems, greenhouses, pumps, and others. Moreover, to address the chattering problem, different sign function approximations are evaluated in the control scheme. Then, the most effective functions for suppressing the chattering phenomenon through extensive comparative analysis are identified. Integral compensation in this technique demonstrates improvement in motor performance, while sign function approximations show a chattering reduction. Different study cases prove the robustness of this control scheme for large-scale synchronous motors. The simulation results validate the proposed control scheme based on sliding modes with integral compensation, by achieving chattering reduction and obtaining an efficient control scheme against uncertain disturbances in synchronous motors for agronomy applications. Full article
(This article belongs to the Special Issue Smart Mechanization and Automation in Agriculture)
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15 pages, 4317 KiB  
Article
Magnetic Aerogels for Room-Temperature Catalytic Production of Bis(indolyl)methane Derivatives
by Nicola Melis, Danilo Loche, Swapneel V. Thakkar, Maria Giorgia Cutrufello, Maria Franca Sini, Gianmarco Sedda, Luca Pilia, Angelo Frongia and Maria Francesca Casula
Molecules 2024, 29(10), 2223; https://doi.org/10.3390/molecules29102223 (registering DOI) - 9 May 2024
Abstract
The potential of aerogels as catalysts for the synthesis of a relevant class of bis-heterocyclic compounds such as bis(indolyl)methanes was investigated. In particular, the studied catalyst was a nanocomposite aerogel based on nanocrystalline nickel ferrite (NiFe2O4) dispersed on amorphous [...] Read more.
The potential of aerogels as catalysts for the synthesis of a relevant class of bis-heterocyclic compounds such as bis(indolyl)methanes was investigated. In particular, the studied catalyst was a nanocomposite aerogel based on nanocrystalline nickel ferrite (NiFe2O4) dispersed on amorphous porous silica aerogel obtained by two-step sol–gel synthesis followed by gel drying under supercritical conditions and calcination treatments. It was found that the NiFe2O4/SiO2 aerogel is an active catalyst for the selected reaction, enabling high conversions at room temperature, and it proved to be active for three repeated runs. The catalytic activity can be ascribed to both the textural and acidic features of the silica matrix and of the nanocrystalline ferrite. In addition, ferrite nanocrystals provide functionality for magnetic recovery of the catalyst from the crude mixture, enabling time-effective separation from the reaction environment. Evidence of the retention of species involved in the reaction into the catalyst is also pointed out, likely due to the porosity of the aerogel together with the affinity of some species towards the silica matrix. Our work contributes to the study of aerogels as catalysts for organic reactions by demonstrating their potential as well as limitations for the room-temperature synthesis of bis(indolyl)methanes. Full article
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21 pages, 3193 KiB  
Article
Serum Plasminogen Activator Inhibitor-1, α 1-Acid Glycoprotein, C-Reactive Protein, and Platelet Factor 4 Levels—Promising Molecules That Can Complete the “Puzzle” of the Biochemical Milieu in Severe Burns: Preliminary Results of a Cohort Prospective Study
by Silviu Constantin Badoiu, Dan Mircea Enescu, Raluca Tatar, Iulia-Ioana Stanescu-Spinu, Daniela Miricescu, Maria Greabu, Ileana Paula Ionel and Viorel Jinga
J. Clin. Med. 2024, 13(10), 2794; https://doi.org/10.3390/jcm13102794 (registering DOI) - 9 May 2024
Abstract
Background: Burns represent a serious health problem, associated with multiple-organ failure, prolonged hospitalization, septic complications, and increased rate of mortality. The main aim of our study was to evaluate the levels of various circulating molecules in children with severe burns (more than [...] Read more.
Background: Burns represent a serious health problem, associated with multiple-organ failure, prolonged hospitalization, septic complications, and increased rate of mortality. The main aim of our study was to evaluate the levels of various circulating molecules in children with severe burns (more than 25% TBSA), in three different moments: 48 h, day 10, and day 21 post-burn. Materials and Methods: This study included 32 children with burns produced by flame, hot liquid, and electric arc and 21 controls. Serum plasminogen activator inhibitor-1 (PAI-1), α 1-acid glycoprotein (AGP), C-reactive protein (CRP), and platelet factor 4 (PF4) were detected using the Multiplex technique. Several parameters, such as fibrinogen, leucocyte count, thrombocyte count, triiodothyronine, thyroxine, and thyroid-stimulating hormone were also determined for each patient during hospitalization. Results: Significant statistical differences were obtained for CRP, AGP, and PF4 compared to the control group, in different moments of measurements. Negative correlations between CRP, AGP, and PF4 serum levels and burned body surface, and also the hospitalization period, were observed. Discussions: CRP levels increased in the first 10 days after burn trauma and then decreased after day 21. Serum PAI-1 levels were higher immediately after the burn and started decreasing only after day 10 post-burn. AGP had elevated levels 48 h after the burn, then decreased at 7–10 days afterwards, and once again increased levels after 21 days. PF4 serum levels increased after day 10 since the burning event. Conclusions: Serum CRP, AGP, PAI-1, and PF4 seem to be promising molecules in monitoring patients with a burn within the first 21 days. Full article
(This article belongs to the Special Issue Advances in Burn Management)
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11 pages, 1008 KiB  
Article
The Cultivation of Spirulina maxima in a Medium Supplemented with Leachate for the Production of Biocompounds: Phycocyanin, Carbohydrates, and Biochar
by Wallyson Ribeiro dos Santos, Matheus Lopes da Silva, Geronimo Virginio Tagliaferro, Ana Lucia Gabas Ferreira and Daniela Helena Pelegrine Guimarães
AgriEngineering 2024, 6(2), 1289-1299; https://doi.org/10.3390/agriengineering6020074 (registering DOI) - 9 May 2024
Abstract
Cyanobacteria are microorganisms that grow rapidly in an aquatic medium, showing the capacity of accumulations of biocompounds subsequently converted into value-added biocompounds. The cyanobacterium Spirulina maxima can produce pigments besides accumulating significant amounts of carbohydrates and proteins. An alternative to reducing biomass production [...] Read more.
Cyanobacteria are microorganisms that grow rapidly in an aquatic medium, showing the capacity of accumulations of biocompounds subsequently converted into value-added biocompounds. The cyanobacterium Spirulina maxima can produce pigments besides accumulating significant amounts of carbohydrates and proteins. An alternative to reducing biomass production costs at an industrial scale is the use of landfill leachate in the growing medium, as well as the mitigation of this pollutant. The objective of this work was to cultivate Spirulina maxima in a medium supplemented with leachate, using the design of experiments to evaluate the effects of leachate concentration (% v/v), light source, and light intensity in an airlift photobioreactor, analyzing them as a response to the productivity of biomass, phycocyanin, carbohydrates, and biochar. The highest values of productivity (mg L−1d−1) were 97.44 ± 3.20, 12.82 ± 0.38, 6.19 ± 1.54, and 34.79 ± 3.62 for biomass, carbohydrates, phycocyanin, and biochar, respectively, adjusted for experiment 2 with the factors of leachate concentration (5.0% v/v), light source (tubular LED), and luminosity (54 µmol m−2 s−1), respectively. The use of leachate as a substitute for macronutrients in Zarrouk’s medium for the cultivation of Spirulina maxima is a viable alternative in the production of biocompounds as long as it is used at an appropriate level. Full article
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11 pages, 2815 KiB  
Communication
Anxiolytic-Like and Antidepressant Effects of a 13H-indolo[2,3-a]thiopyrano[2,3-g]quinolizine Derivative
by Carlos E. Castillo-Espinoza, María Leonor González-Rivera, Alberto Medina-Ortiz, Juan Carlos Barragan-Galvez, Sergio Hidalgo-Figueroa, David Cruz Cruz, Martha Alicia Deveze-Alvarez, Gerardo González-García, Clarisa Villegas Gómez and Angel Josabad Alonso-Castro
Chemistry 2024, 6(3), 376-386; https://doi.org/10.3390/chemistry6030022 (registering DOI) - 9 May 2024
Abstract
Depressive and anxiety disorders constitute some of the most prevalent mental disorders around the world. For years, the development of new lead compounds for drug discovery in this field has been an area of great attention. Recently, a series of tetrahydrocarbazole derivatives have [...] Read more.
Depressive and anxiety disorders constitute some of the most prevalent mental disorders around the world. For years, the development of new lead compounds for drug discovery in this field has been an area of great attention. Recently, a series of tetrahydrocarbazole derivatives have demonstrated important anxiolytic-like activity, associated with their structures and stereochemistry. Here, we present a study of the antidepressant effect and anxiolytic-like activity of a fused thiopyrano-piperidone-tetrahydrocarboline (compound 4). The antidepressant and anxiolytic-like effects of 4 (1–50 mg/kg p.o.) were assessed with the tail suspension test and the hole-board test, respectively. This study determined the possible mechanisms involved in the anxiolytic-like actions of 4 using inhibitors or neurotransmission and evaluated its interaction with 5HT2A receptors using a molecular docking study. As an analog to the tetrahydrocarbazole core, the tetrahydrocarboline derivative showed anxiolytic-like activity (ED50 = 13 mg/kg p.o.) in the hole-board test, with a comparable effect to the reference drug, 1.5 mg/kg clonazepam, with the possible participation of the serotonergic system. Full article
(This article belongs to the Section Medicinal Chemistry)
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16 pages, 3203 KiB  
Communication
Endotypes of Chronic Rhinosinusitis with Primary and Recurring Nasal Polyps in the Latvian Population
by Rudolfs Janis Viksne, Gunta Sumeraga and Mara Pilmane
Int. J. Mol. Sci. 2024, 25(10), 5159; https://doi.org/10.3390/ijms25105159 (registering DOI) - 9 May 2024
Abstract
Chronic rhinosinusitis (CRS) is a complex syndrome with various inflammatory mechanisms resulting in different patterns of inflammation that correlate with the clinical phenotypes of CRS. Our aim was to use detected IL-1, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, Ki 67, HBD-2, HBD-3, and [...] Read more.
Chronic rhinosinusitis (CRS) is a complex syndrome with various inflammatory mechanisms resulting in different patterns of inflammation that correlate with the clinical phenotypes of CRS. Our aim was to use detected IL-1, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, Ki 67, HBD-2, HBD-3, and LL-37 to classify specific inflammatory endotypes in chronic rhinosinusitis with the tissue of nasal polyps (CRSwNP). Samples from 35 individuals with primary and recurrent CRSwNP were taken during surgery. The tissues were stained for the previously mentioned biomarkers immunohistochemically. A hierarchical cluster analysis was performed. The clinical parameters were compared between clusters. Five clusters had significantly different biomarkers between groups. There were no significant differences in the clinical parameters, except for the Lund–Mackay score, which was significantly higher in cluster 4 compared to that of cluster 1 (p = 0.024). Five endotypes of (CRSwNP) are characterized by different combinations of type 1, type 2, and type 3 tissue inflammation patterns. In the Latvian population, endotypes associated with neutrophilic inflammation or a combination of neutrophilic inflammation and type 2 inflammation are predominant. Increased proliferation marker Ki 67 values are not associated with more severe inflammation in the tissue samples of chronic rhinosinusitis with nasal polyps. Full article
(This article belongs to the Special Issue Chronic Rhinosinusitis: Aetiology, Immunology and Treatment 2.0)
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14 pages, 4065 KiB  
Article
Exploring the Regulators of Keratinization: Role of BMP-2 in Oral Mucosa
by Xindi Mu, Mitsuaki Ono, Ha Thi Thu Nguyen, Ziyi Wang, Kun Zhao, Taishi Komori, Tomoko Yonezawa, Takuo Kuboki and Toshitaka Oohashi
Cells 2024, 13(10), 807; https://doi.org/10.3390/cells13100807 - 9 May 2024
Abstract
The oral mucosa functions as a physico-chemical and immune barrier to external stimuli, and an adequate width of the keratinized mucosa around the teeth or implants is crucial to maintaining them in a healthy and stable condition. In this study, for the first [...] Read more.
The oral mucosa functions as a physico-chemical and immune barrier to external stimuli, and an adequate width of the keratinized mucosa around the teeth or implants is crucial to maintaining them in a healthy and stable condition. In this study, for the first time, bulk RNA-seq analysis was performed to explore the gene expression of laser microdissected epithelium and lamina propria from mice, aiming to investigate the differences between keratinized and non-keratinized oral mucosa. Based on the differentially expressed genes (DEGs) and Gene Ontology (GO) Enrichment Analysis, bone morphogenetic protein 2 (BMP-2) was identified to be a potential regulator of oral mucosal keratinization. Monoculture and epithelial–mesenchymal cell co-culture models in the air–liquid interface (ALI) indicated that BMP-2 has direct and positive effects on epithelial keratinization and proliferation. We further performed bulk RNA-seq of the ALI monoculture stimulated with BMP-2 in an attempt to identify the downstream factors promoting epithelial keratinization and proliferation. Analysis of the DEGs identified, among others, IGF2, ID1, LTBP1, LOX, SERPINE1, IL24, and MMP1 as key factors. In summary, these results revealed the involvement of a well-known growth factor responsible for bone development, BMP-2, in the mechanism of oral mucosal keratinization and proliferation, and pointed out the possible downstream genes involved in this mechanism. Full article
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20 pages, 458 KiB  
Review
Current Oncology Nutrition Care Practice in Southeast Asia: A Scoping Review
by Choirun Nissa, Lauren Hanna and Judy Bauer
Nutrients 2024, 16(10), 1427; https://doi.org/10.3390/nu16101427 (registering DOI) - 9 May 2024
Abstract
Although evidence-based nutrition care is recommended for patients with cancer, current nutrition care practices provided by nutritionists and dietitians in Southeast Asian countries are not clearly reported. The aim of this scoping review was to describe nutritionists’ and dietitians’ current oncology nutrition care [...] Read more.
Although evidence-based nutrition care is recommended for patients with cancer, current nutrition care practices provided by nutritionists and dietitians in Southeast Asian countries are not clearly reported. The aim of this scoping review was to describe nutritionists’ and dietitians’ current oncology nutrition care practice within Southeast Asia by identifying access to dietetic services, tools or strategies used in providing care, and barriers and enablers to implementing nutrition care practices. Five databases (Ovid MEDLINE, Global Health, Embase, Cochrane Central Register of Controlled Trials, and Proquest) were searched through structured search strategies, in addition to strategic searching of grey literature. A total of 4261 sources of evidence were retrieved. After full-text screening, 18 studies from Southeast Asian countries met the inclusion criteria and were included in this review. The provision and reporting of nutrition care practices provided by nutritionists and dietitians were limited. Access to dietetic services, including nutritional screening tools and reason to be referred, were varied within studies. Barriers and enablers to nutrition care provision were unique and related to each country’s specific resources and guidelines. In summary, there was varied reporting of nutrition care practices provided to patients with cancer in Southeast Asia and a lack of clarity on the actual standardized processes. Future research is warranted to further explore the barriers and enablers to providing nutrition care by local nutritionists and dietitians in Southeast Asia. Full article
(This article belongs to the Section Clinical Nutrition)
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13 pages, 2998 KiB  
Technical Note
Image Quality Assessment Tool for Conventional and Dynamic Magnetic Resonance Imaging Acquisitions
by Katerina Nikiforaki, Ioannis Karatzanis, Aikaterini Dovrou, Maciej Bobowicz, Katarzyna Gwozdziewicz, Oliver Díaz, Manolis Tsiknakis, Dimitrios I. Fotiadis, Karim Lekadir and Kostas Marias
J. Imaging 2024, 10(5), 115; https://doi.org/10.3390/jimaging10050115 (registering DOI) - 9 May 2024
Abstract
Image quality assessment of magnetic resonance imaging (MRI) data is an important factor not only for conventional diagnosis and protocol optimization but also for fairness, trustworthiness, and robustness of artificial intelligence (AI) applications, especially on large heterogeneous datasets. Information on image quality in [...] Read more.
Image quality assessment of magnetic resonance imaging (MRI) data is an important factor not only for conventional diagnosis and protocol optimization but also for fairness, trustworthiness, and robustness of artificial intelligence (AI) applications, especially on large heterogeneous datasets. Information on image quality in multi-centric studies is important to complement the contribution profile from each data node along with quantity information, especially when large variability is expected, and certain acceptance criteria apply. The main goal of this work was to present a tool enabling users to assess image quality based on both subjective criteria as well as objective image quality metrics used to support the decision on image quality based on evidence. The evaluation can be performed on both conventional and dynamic MRI acquisition protocols, while the latter is also checked longitudinally across dynamic series. The assessment provides an overall image quality score and information on the types of artifacts and degrading factors as well as a number of objective metrics for automated evaluation across series (BRISQUE score, Total Variation, PSNR, SSIM, FSIM, MS-SSIM). Moreover, the user can define specific regions of interest (ROIs) to calculate the regional signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR), thus individualizing the quality output to specific use cases, such as tissue-specific contrast or regional noise quantification. Full article
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20 pages, 613 KiB  
Article
Nonstandard Nearly Exact Analysis of the FitzHugh–Nagumo Model
by Shahid, Mujahid Abbas and Eddy Kwessi
Symmetry 2024, 16(5), 585; https://doi.org/10.3390/sym16050585 (registering DOI) - 9 May 2024
Abstract
The FitzHugh–Nagumo model has been used empirically to model certain types of neuronal activities. It is also a non-linear dynamical system applicable to chemical kinetics, population dynamics, epidemiology and pattern formation. In the literature, many approaches have been proposed to study its dynamics. [...] Read more.
The FitzHugh–Nagumo model has been used empirically to model certain types of neuronal activities. It is also a non-linear dynamical system applicable to chemical kinetics, population dynamics, epidemiology and pattern formation. In the literature, many approaches have been proposed to study its dynamics. In this paper, initially, we have employed cutting-edge tools from discrete dynamics for discretization and fixed points. It has been proven that an exact discrete scheme exists for this paradigm. This project also considers the phase space and integral surfaces of these evolutionary equations. In addition, it carries out a thorough symmetry analysis of this reaction diffusion system to find equivalent systems. Moreover, steady-state solutions are obtained using ansatzes for traveling wave solutions. The existence of infinite traveling wave solutions has also been proven. Yet again, this investigation establishes the potential of symmetry methods to unravel non-linearity. Finally, singular perturbation theory has been employed to obtain analytical approximations and to study stability in different parameter regimes. Full article
(This article belongs to the Special Issue Nonlinear Symmetric Systems and Chaotic Systems in Engineering)
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18 pages, 3736 KiB  
Article
Bovine Serum Albumin Effect on Collapsing PNIPAM Chains in Aqueous Solutions: Spin Label and Spin Probe Study
by Georgii A. Simenido, Ekaterina M. Zubanova, Evgenii A. Ksendzov, Sergei V. Kostjuk, Peter S. Timashev and Elena N. Golubeva
Polymers 2024, 16(10), 1335; https://doi.org/10.3390/polym16101335 (registering DOI) - 9 May 2024
Abstract
The influence of bovine serum albumin (BSA) on collapsing poly(N-isopropylacrylamide) (PNIPAM) chains was studied with turbidimetry and spin probe and spin label electron paramagnetic resonance spectroscopy. An increased ratio of collapsed chains in aqueous solutions in the narrow temperature region near [...] Read more.
The influence of bovine serum albumin (BSA) on collapsing poly(N-isopropylacrylamide) (PNIPAM) chains was studied with turbidimetry and spin probe and spin label electron paramagnetic resonance spectroscopy. An increased ratio of collapsed chains in aqueous solutions in the narrow temperature region near the LCST appeared in the presence of 2.5–10 wt% BSA. The spin probe EPR data indicate that the inner cavities of the BSA dimers are probably responsive to the capture of small hydrophobic or amphiphilic molecules, such as TEMPO nitroxyl radical. The observed features of the structure and dynamics of inhomogeneities of aqueous PNIPAM-BSA solutions, including their mutual influence on the behavior of the polymer and protein below the LCST, should be considered when developing and investigating PNIPAM-based drug delivery systems. Full article
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18 pages, 8152 KiB  
Review
Heart Failure with Mildly Reduced Ejection Fraction—A Phenotype Waiting to Be Explored
by Anugrah Nair, Lukah Q. Tuan, Natasha Jones-Lewis, Deep Chandh Raja, Jenish Shroff and Rajeev Kumar Pathak
J. Cardiovasc. Dev. Dis. 2024, 11(5), 148; https://doi.org/10.3390/jcdd11050148 (registering DOI) - 9 May 2024
Abstract
Heart failure (HF) presents a significant global health challenge recognised by frequent hospitalisation and high mortality rates. The assessment of left ventricular (LV) ejection fraction (EF) plays a crucial role in diagnosing and predicting outcomes in HF, leading to its classification into preserved [...] Read more.
Heart failure (HF) presents a significant global health challenge recognised by frequent hospitalisation and high mortality rates. The assessment of left ventricular (LV) ejection fraction (EF) plays a crucial role in diagnosing and predicting outcomes in HF, leading to its classification into preserved (HFpEF), reduced (HFrEF), and mildly reduced (HFmrEF) EF. HFmrEF shares features of both HFrEF and HFpEF but also exhibits distinct characteristics. Despite advancements, managing HFmrEF remains challenging due to its diverse presentation. Large-scale studies are needed to identify the predictors of clinical outcomes and treatment responses. Utilising biomarkers for phenotyping holds the potential for discovering new treatment targets. Given the uncertainty surrounding optimal management, individualised approaches are imperative for HFmrEF patients. This chapter examines HFmrEF, discusses the rationale for its re-classification, and elucidates HFmrEF’s key attributes. Furthermore, it provides a comprehensive review of current treatment strategies for HFmrEF patients. Full article
(This article belongs to the Section Electrophysiology and Cardiovascular Physiology)
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20 pages, 4070 KiB  
Review
Induction of Hepatoma Cell Pyroptosis by Endogenous Lipid Geranylgeranoic Acid—A Comparison with Palmitic Acid and Retinoic Acid
by Yoshihiro Shidoji
Cells 2024, 13(10), 809; https://doi.org/10.3390/cells13100809 (registering DOI) - 9 May 2024
Abstract
Research on retinoid-based cancer prevention, spurred by the effects of vitamin A deficiency on gastric cancer and subsequent clinical studies on digestive tract cancer, unveils novel avenues for chemoprevention. Acyclic retinoids like 4,5-didehydrogeranylgeranoic acid (4,5-didehydroGGA) have emerged as potent agents against hepatocellular carcinoma [...] Read more.
Research on retinoid-based cancer prevention, spurred by the effects of vitamin A deficiency on gastric cancer and subsequent clinical studies on digestive tract cancer, unveils novel avenues for chemoprevention. Acyclic retinoids like 4,5-didehydrogeranylgeranoic acid (4,5-didehydroGGA) have emerged as potent agents against hepatocellular carcinoma (HCC), distinct from natural retinoids such as all-trans retinoic acid (ATRA). Mechanistic studies reveal GGA’s unique induction of pyroptosis, a rapid cell death pathway, in HCC cells. GGA triggers mitochondrial superoxide hyperproduction and ER stress responses through Toll-like receptor 4 (TLR4) signaling and modulates autophagy, ultimately activating pyroptotic cell death in HCC cells. Unlike ATRA-induced apoptosis, GGA and palmitic acid (PA) induce pyroptosis, underscoring their distinct mechanisms. While all three fatty acids evoke mitochondrial dysfunction and ER stress responses, GGA and PA inhibit autophagy, leading to incomplete autophagic responses and pyroptosis, whereas ATRA promotes autophagic flux. In vivo experiments demonstrate GGA’s potential as an anti-oncometabolite, inducing cell death selectively in tumor cells and thus suppressing liver cancer development. This review provides a comprehensive overview of the molecular mechanisms underlying GGA’s anti-HCC effects and underscores its promising role in cancer prevention, highlighting its importance in HCC prevention. Full article
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17 pages, 270 KiB  
Article
Birth–Death Processes with Two-Type Catastrophes
by Junping Li
Mathematics 2024, 12(10), 1468; https://doi.org/10.3390/math12101468 (registering DOI) - 9 May 2024
Abstract
This paper concentrates on the general birth–death processes with two different types of catastrophes. The Laplace transform of transition probability function for birth–death processes with two-type catastrophes is successfully expressed with the Laplace transform of transition probability function of the birth–death processes without [...] Read more.
This paper concentrates on the general birth–death processes with two different types of catastrophes. The Laplace transform of transition probability function for birth–death processes with two-type catastrophes is successfully expressed with the Laplace transform of transition probability function of the birth–death processes without catastrophe. The first effective catastrophe occurrence time is considered. The Laplace transform of its probability density function, expectation and variance are obtained. Full article
(This article belongs to the Special Issue Probability, Statistics and Random Processes)
20 pages, 1277 KiB  
Article
How Power Affects Moral Judgments: The Presence of Harm to Life Modifies the Association between Power and Moral Choices
by Mufan Zheng, Ana Guinote and Wei Luo
Soc. Sci. 2024, 13(5), 256; https://doi.org/10.3390/socsci13050256 (registering DOI) - 9 May 2024
Abstract
Lammers and Stapel reported that high power increases deontological (rule-based) moral thinking, and low power increases utilitarian (outcome-based) moral thinking. However, the dilemmas were mild and did not involve harm to life. Here, we examined whether the presence or absence of harm to [...] Read more.
Lammers and Stapel reported that high power increases deontological (rule-based) moral thinking, and low power increases utilitarian (outcome-based) moral thinking. However, the dilemmas were mild and did not involve harm to life. Here, we examined whether the presence or absence of harm to life affects the moral decisions of powerholders. To help establish the replicability and validity of the effects of power on moral judgments in the absence of harm to life, we first performed an exact replication of a study conducted by Lammers and Stapel, and this experiment was followed up by a similar study in an organizational context in China (Studies 1 and 2). Studies 3 and 4 investigated whether power and the presence/absence of harm to life interacted with preferences for deontological versus utilitarian moral judgments. Power consistently triggered deontological thinking. However, power differences in moral reasoning only emerged when there was no harm to life. Harm prompted deontological responses among control and powerless individuals, which nullified differences across the power conditions. The findings demarcate the generalizability of the association between power and a moral thinking style. Full article
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25 pages, 5319 KiB  
Article
Analysis of Selected Toll-like Receptors in the Pathogenesis and Advancement of Non-Small-Cell Lung Cancer
by Jolanta Smok-Kalwat, Paulina Mertowska, Sebastian Mertowski, Stanisław Góźdź, Izabela Korona-Głowniak, Wojciech Kwaśniewski and Ewelina Grywalska
J. Clin. Med. 2024, 13(10), 2793; https://doi.org/10.3390/jcm13102793 (registering DOI) - 9 May 2024
Abstract
(1) Background: Non-small-cell lung cancer (NSCLC) represents a significant global health challenge, contributing to numerous cancer deaths. Despite advances in diagnostics and therapy, identifying reliable biomarkers for prognosis and therapeutic stratification remains difficult. Toll-like receptors (TLRs), crucial for innate immunity, now show potential [...] Read more.
(1) Background: Non-small-cell lung cancer (NSCLC) represents a significant global health challenge, contributing to numerous cancer deaths. Despite advances in diagnostics and therapy, identifying reliable biomarkers for prognosis and therapeutic stratification remains difficult. Toll-like receptors (TLRs), crucial for innate immunity, now show potential as contributors to cancer development and progression. This study aims to investigate the role of TLR expression as potential biomarkers in the development and progression of NSCLC. (2) Materials and Methods: The study was conducted on 89 patients diagnosed with NSCLC and 40 healthy volunteers, for whom the prevalence of TLR2, TLR3, TLR4, TLR7, TLR8, and TLR9 was assessed on selected subpopulations of T and B lymphocytes in the peripheral blood of recruited patients along with the assessment of their serum concentration. (3) Result: Our study showed several significant changes in NSCLC patients at the beginning of the study. This resulted in a 5-year follow-up of changes in selected TLRs in recruited patients. Due to the high mortality rate of NSCLC patients, only 16 patients survived the 5 years. (4) Conclusions: The results suggest that TLRs may constitute real biomarker molecules that may be used for future prognostic purposes in NSCLC. However, further validation through prospective clinical and functional studies is necessary to confirm their clinical utility. These conclusions may lead to better risk stratification and tailored interventions, benefiting NSCLC patients and bringing medicine closer to precision. Full article
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19 pages, 12306 KiB  
Article
Towards Complex Tissues Replication: Multilayer Scaffold Integrating Biomimetic Nanohydroxyapatite/Chitosan Composites
by Barbara Palazzo, Stefania Scialla, Amilcare Barca, Laura Sercia, Daniela Izzo, Francesca Gervaso and Francesca Scalera
Bioengineering 2024, 11(5), 471; https://doi.org/10.3390/bioengineering11050471 (registering DOI) - 9 May 2024
Abstract
This study explores an approach to design and prepare a multilayer scaffold mimicking interstratified natural tissue. This multilayer construct, composed of chitosan matrices with graded nanohydroxyapatite concentrations, was achieved through an in situ biomineralization process applied to individual layers. Three distinct precursor concentrations [...] Read more.
This study explores an approach to design and prepare a multilayer scaffold mimicking interstratified natural tissue. This multilayer construct, composed of chitosan matrices with graded nanohydroxyapatite concentrations, was achieved through an in situ biomineralization process applied to individual layers. Three distinct precursor concentrations were considered, resulting in 10, 20, and 30 wt% nanohydroxyapatite content in each layer. The resulting chitosan/nanohydroxyapatite (Cs/n-HAp) scaffolds, created via freeze-drying, exhibited nanohydroxyapatite nucleation, homogeneous distribution, improved mechanical properties, and good cytocompatibility. The cytocompatibility analysis revealed that the Cs/n-HAp layers presented cell proliferation similar to the control in pure Cs for the samples with 10% n-HAp, indicating good cytocompatibility at this concentration, while no induction of apoptotic death pathways was demonstrated up to a 20 wt% n-Hap concentration. Successful multilayer assembly of Cs and Cs/n-HAp layers highlighted that the proposed approach represents a promising strategy for mimicking multifaceted tissues, such as osteochondral ones. Full article
(This article belongs to the Special Issue Biomaterials for Cartilage and Bone Tissue Engineering)
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11 pages, 2226 KiB  
Article
Performance of Aubergine Rootstocks against Verticillium dahliae Isolates in Southeastern Spain
by Carmen María Lacasa, Manuel Cantó-Tejero, Victoriano Martínez, Alfredo Lacasa and Pedro Guirao
Agronomy 2024, 14(5), 998; https://doi.org/10.3390/agronomy14050998 (registering DOI) - 9 May 2024
Abstract
Aubergine (Solanum melongena L.) (Solanaceae) is a widespread crop in the Mediterranean basin. Verticillium dahliae is one of the main soil-borne pathogens affecting the aubergine crop. Its control has traditionally been achieved by soil fumigation with chemical disinfectants. Restrictions on the use [...] Read more.
Aubergine (Solanum melongena L.) (Solanaceae) is a widespread crop in the Mediterranean basin. Verticillium dahliae is one of the main soil-borne pathogens affecting the aubergine crop. Its control has traditionally been achieved by soil fumigation with chemical disinfectants. Restrictions on the use of chemical fumigants have led to the search for solutions in genetic resistance using rootstocks. In southeastern Spain, aubergines are grafted for the control of V. dahliae. Two Solanum torvum rootstocks (Hugo F1 and Torpedo) and a Solanum melongena hybrid (Javah F1) were tested against five isolates of V. dahliae obtained from grafted (A1 and A2) and ungrafted (Vd8, Vd17 and Vd66) aubergines compared with the susceptible cultivar Larne F1 under controlled conditions. Isolates from grafted plants infected all three rootstocks, with differences observed in the percentage of plants with symptoms and in the disease symptom severity. Three strains isolated from the ungrafted aubergines (Vd8, Vd17 and Vd66) infected Javah F1 rootstock. The Hugo F1 and Torpedo rootstocks showed a high level of resistance to V. dahliae, while Javah F1 was susceptible to the pathogen. The Hugo F1 and Torpedo rootstocks are suitable for mitigating the effects of Verticillium wilt in Mediterranean aubergine crops. Understanding the nature of the resistance from S. torvum could enhance the benefits of grafting or facilitate the introduction of resistance into commercial cultivars. Full article
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22 pages, 6455 KiB  
Article
Immune Rejection Mediated by prf1 and gzmb Affects the Colonization of Fat Greenling (Hexagrammos otakii) Spermatogonia in Heterotransplantation
by Xi Zhao, Ying Chen, Rui Li, Yu Men, Kai Yan, Zibin Li, Wenxiu Cai, Yan He and Jie Qi
Int. J. Mol. Sci. 2024, 25(10), 5157; https://doi.org/10.3390/ijms25105157 (registering DOI) - 9 May 2024
Abstract
Fish germ cell transplantation holds great potential for conserving endangered species, improving cultured fish breeds, and exploring reproductive techniques. However, low transplantation efficiency is a common issue in heterotransplantation. This study transplanted fat greenling (Hexagrammos otakii) spermatogonia into the testes of [...] Read more.
Fish germ cell transplantation holds great potential for conserving endangered species, improving cultured fish breeds, and exploring reproductive techniques. However, low transplantation efficiency is a common issue in heterotransplantation. This study transplanted fat greenling (Hexagrammos otakii) spermatogonia into the testes of spotted sea bass (Lateolabrax maculatus) to investigate factors that might affect the colonization and fixation of heterologous transplanted germ cells. Results indicated that transplanted fat greenling spermatogonia cells were successfully detected in the early transplantation phase in spotted sea bass. Their numbers gradually decreased over time, and after 10 days post-transplantation, more than 90% of the transplanted cells underwent apoptosis. Transcriptome sequencing analysis of the testes of spotted sea bass and fat greenling spermatogonia on days 1 and 10 post-transplantation revealed that this apoptosis process involved many immune-related genes and their associated signaling pathways. Acute immune rejection marker genes prf1 and gzmb were detected in the spotted sea bass testes, while immune tolerance genes lck and zap-70 were expressed in the fat greenling spermatogonia. Additionally, differential expression of prf1 and gzmb genes was screened from spotted sea bass, with experimental evidence indicating that PRF1 and GZMB protein from spotted sea bass primarily induce apoptosis in transplanted fat greenling spermatogonia via the mitochondrial apoptosis pathway, at the protein level. This suggests that the difficulties in heterotransplantation are primarily related to acute immune rejection, with PRF1 and GZMB playing significant roles. Full article
(This article belongs to the Section Molecular Immunology)
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12 pages, 2921 KiB  
Article
Study of Acoustic Emission from the Gate of Gallium Nitride High Electron Mobility Transistors
by Bartłomiej K. Paszkiewicz, Bogdan Paszkiewicz and Andrzej Dziedzic
Electronics 2024, 13(10), 1840; https://doi.org/10.3390/electronics13101840 (registering DOI) - 9 May 2024
Abstract
Nitrides are the leading semiconductor material used for the fabrication of high electron mobility transistors (HEMTs). They exhibit piezoelectric properties, which, coupled with their high mechanical stiffness, expand their versatile applications into the fabrication of piezoelectric devices. Today, due to advances in device [...] Read more.
Nitrides are the leading semiconductor material used for the fabrication of high electron mobility transistors (HEMTs). They exhibit piezoelectric properties, which, coupled with their high mechanical stiffness, expand their versatile applications into the fabrication of piezoelectric devices. Today, due to advances in device technology that result in a reduction in the size of individual transistor elements and due to increased structural complexity (e.g., multi-gate transistors), the integration of piezoelectric materials into HEMTs leads to an interesting occurrence, namely acoustic emission from the transistor gate due to piezoelectric effects. This could affect the device’s performance, reliability, and durability. However, this phenomenon has not yet been comprehensively described. This paper aims to examine this overlooked aspect of AlGaN/GaN HEMT operation, that is, the acoustic emission from the gate region of the device induced by piezoelectric effects. For this purpose, dedicated test structures were designed, consisting of two narrow 1.7 μm-wide metallization strips placed at distances ranging from 5 μm to 200 μm fabricated in AlGaN/GaN heterostructures to simulate and examine the gate behavior of the HEMT transistor. For comparison, the test device structures were also fabricated on sapphire, which is not a piezoelectric material. Measurements of acoustic and electrical interactions in the microwave range were carried out using the “on wafer” method with Picoprobe’s signal–ground–signal (SGS)-type microwave probes. The dependence of reflectance |S11| and transmittance |S21| vs. frequency was investigated, and the coupling capacitance was determined. An equivalent circuit model of the test structure was developed, and finite element method simulation was performed to study the distribution of the acoustic wave in the nitride layers and substrate for different frequencies using Comsol Multiphysics software. At frequencies up to 2–3 GHz, the formation of volume waves and a surface wave, capable of propagating over long distances (in the order of tens of micrometers) was observed. At higher frequencies, the resulting distribution of displacements as a result of numerous reflections and interferences was more complicated. However, there was always the possibility of a surface wave occurrence, even at large distances from the excitation source. At small gate distances, electrical interactions dominate. Above 100 µm, electrical interactions are comparable to acoustic ones. With further increases in distance, weakly attenuated surface waves will dominate. Full article
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