White organic light-emitting diodes (WOLEDs) hold vast prospects in the fields of next-generation displays and solid-state lighting. Ultrathin emitting layers (UEMLs) have become a research hotspot because of their unique advantage. On the basis of simplifying the device structure and preparation process, they can achieve electroluminescent performance comparable to that of doped devices. In this review, we first discuss the working principles and advantages of WOLEDs based on UEML architecture, which can achieve low cost and more flexibility by simplifying the device structure and preparation process. Subsequently, the successful applications of doping and non-doping technologies in fluorescent, phosphorescent, and hybrid WOLEDs combined with UEMLs are discussed, and the operation mechanisms of these WOLEDs are emphasized briefly. We firmly believe that this article will bring new hope for the development of UEML-based WOLEDs in the future. Full article

Periodontitis is linked to the onset and progression of oral squamous cell carcinoma (OSCC), an epidemiologically frequent and clinically aggressive malignancy. In this context, Fusobacterium (F.) nucleatum and Porphyromonas (P.) gingivalis, two bacteria that cause periodontitis, are found in OSCC tissues as well as in oral premalignant lesions, where they exert pro-tumorigenic activities. Since the two bacteria are present also in endodontic diseases, playing a role in their pathogenesis, here we analyze the literature searching for information on the impact that endodontic infection by P. gingivalis or F. nucleatum could have on cellular and molecular events involved in oral carcinogenesis. Results from the reviewed papers indicate that infection by P. gingivalis and/or F. nucleatum triggers the production of inflammatory cytokines and growth factors in dental pulp cells or periodontal cells, affecting the survival, proliferation, invasion, and differentiation of OSCC cells. In addition, the two bacteria and the cytokines they induce halt the differentiation and stimulate the proliferation and invasion of stem cells populating the dental pulp or the periodontium. Although most of the literature confutes the possibility that bacteria-induced endodontic inflammatory diseases could impact on oral carcinogenesis, the papers we have analyzed and discussed herein recommend further investigations on this topic. Full article

In genomic studies, univariate analysis is commonly used to discover susceptible variants. It applies univariate regression for each variant and tests the significance of the regression coefficient or slope parameter. This strategy, however, may miss signals that are jointly detectable with other variants. Multivariate analysis is another popular approach, which tests grouped variants with a predefined group, e.g., based on a gene, pathway, or physical location. However, the power will be diminished if the modeling assumption is not suited to the data. Therefore, data-adaptive testing that relies on fewer modeling assumptions is preferable. Possible approaches include a data-adaptive test proposed by Ueki (2021), which applies to various data-adaptive regression models using a generalization of Yanai’s generalized coefficient of determination. While several regression models are possible choices for the data-adaptive test, this paper focuses on the fused lasso that can count for the effect of adjacent variants and investigates its performance through comparison with other existing tests. Simulation studies demonstrate that the test using fused lasso has a high power compared to the existing tests including the univariate regression test, saturated regression test, SKAT (sequence kernel association test), burden test, SKAT-O (optimized sequence kernel association test), and the tests using lasso, ridge, and elastic net when assuming a similar effect of adjacent variants. Full article

Bend sections are ubiquitous in natural sandy river systems. This study employs Computational Fluid Dynamics–Discrete Phase Model (CFD-DPM) methodology to analyze particle transport dynamics in U-bend channel flows, focusing on the distinctions between partially vegetated (Case No.1) and non-vegetated (Case No.2) scenarios. The research aims to unravel the intricate relationships among bending channel-induced secondary flow, vegetation blockage, and particle aggregation, employing both quantitative and qualitative approaches. (I) The key findings reveal that vegetation near the inner walls of curved channels markedly diminishes the intensity of secondary circulation. This reduction in circulation intensity is observed not only within vegetated areas but also extends to adjacent non-vegetated zones. Additionally, the study identifies a close correlation between vertical vortices and particle distribution near the channel bed. While particle distribution generally aligns with the vortices’ margin, dynamic patch-scale eddies near vegetation patches induce deviations, creating wave-like patterns in particle distribution. (II) The application of the Probability Density Function (PDF) provides insights into the radius-wise particle distribution. In non-vegetated channels, particle distribution is primarily influenced by secondary flow and boundary layers. In contrast, the presence of vegetation leads to a complex mixing layer, altering the particle distribution pattern and maximizing PDF values in non-vegetated free flow subzones. (III) Furthermore, the research quantifies spatial–temporal sediment heterogeneity through PDF variance. The findings demonstrate that variance in non-vegetated channels increases towards the outer wall in bending regions. Vegetation-induced turbulence causes higher variance, particularly in the mixing layer subzone, underscoring the significance of eddy size in sediment redistribution. (IV) The study of vertical concentration profiles in vegetated U-bend channels offers additional insights, while secondary flow in non-vegetated channels facilitates upward sediment transport and vegetation presence, although increasing the Turbulent Kinetic Energy (TKE), restricts channel space, and impedes secondary flow, thereby reducing vertical particle suspension. Sediment concentrations are found to be higher in the lower layers of vegetated bends, contrary to the pattern in non-vegetated bends. These findings highlight the complex interplay between vegetation, secondary flow, and sediment transport, illustrating the reduced effectiveness of secondary flow in promoting vertical particle transportation in bending channels due to the vegetation obstruction. Full article

The COVID-19 pandemic has affected the lifestyles of people of all ages, conditions and occupations. Social distance, remote working, changes in diet and a lack of physical activity have directly and indirectly affected many aspects of mental and physical health, particularly in patients with many comorbidities and non-communicable diseases (NCDs). In our paper, we analyzed COVID-19 hospitalized and non-hospitalized cases according to comorbidities to assess the average monthly percentage change (AMPC) and monthly percentage change (MPC) using open access data from the Chilean Ministry of Science, Technology, Knowledge and Innovation. As expected, the infection mainly affected patients with comorbidities, including cardiovascular risk factors. The hospitalized cases with obesity and chronic lung disease increased throughout the period of June 2020–August 2021 (AMPC = ↑20.8 and ↑19.4%, respectively, p < 0.05), as did all the non-hospitalized cases with comorbidities throughout the period (AMPC = ↑15.6 to ↑30.3 [p < 0.05]). The increases in hospitalizations and non-hospitalizations with comorbidities may be associated with physical inactivity. A healthy lifestyle with regular physical activity may have had a protective effect on the COVID-19 severity and related events in the post-pandemic period, especially for the NCD population. Full article

In the realm of thermal interface materials (TIMs), high thermal conductivity and low density are key for effective thermal management and are particularly vital due to the growing compactness and lightweight nature of electronic devices. Efficient directional arrangement is a key control strategy to significantly improve thermal conductivity and comprehensive properties of thermal interface materials. In the present work, drawing inspiration from natural leaf and branch structures, a simple-to-implement approach for fabricating oriented thermal conductivity composites is introduced. Utilizing carbon fibers (CFs), known for their ultra-high thermal conductivity, as branches, this design ensures robust thermal conduction channels. Concurrently, boron nitride (BN) platelets, characterized by their substantial in-plane thermal conductivity, act as leaves. These components not only support the branches but also serve as junctions in the thermal conduction network. Remarkably, the composite achieves a thermal conductivity of 11.08 W/(m·K) with just an 11.1 wt% CF content and a 1.86 g/cm³ density. This study expands the methodologies for achieving highly oriented configurations of fibrous and flake materials, which provides a new design idea for preparing high-thermal conductivity and low-density thermal interface materials. Full article

Xanthates have long been described as poor RAFT/MADIX agents for styrene polymerization. Through the determination of chain transfer constants to xanthates, this work demonstrated beneficial capto-dative substituent effects for the leaving group of a new series of α-amido trifluoromethyl xanthates, with the best effect observed with trifluoroacetyl group. The previously observed Z-group activation with a O-trifluoroethyl group compared to the O-ethyl counterpart was quantitatively established with C_ex = 2.7 (3–4 fold increase) using the SEC peak resolution method. This study further confirmed the advantageous incorporation of trifluoromethyl substituents to activate xanthates in radical chain transfer processes and contributed to identify the most reactive xanthate reported to date for RAFT/MADIX polymerization of styrene. Full article

Ilex × attenuata ‘Sunny Foster’ represents a yellow leaf mutant originating from I. × attenuata ‘Foster#2’, a popular ornamental woody cultivar. However, the molecular mechanisms underlying this leaf color mutation remain unclear. Using a comprehensive approach encompassing cytological, physiological, and transcriptomic methodologies, notable distinctions were discerned between the mutant specimen and its wild type. The mutant phenotype displayed aberrant chloroplast morphology, diminished chlorophyll content, heightened carotenoid/chlorophyll ratios, and a decelerated rate of plant development. Transcriptome analysis identified differentially expressed genes (DEGs) related to chlorophyll metabolism, carotenoid biosynthesis and photosynthesis. The up-regulation of CHLD and CHLI subunits leads to decreased magnesium chelatase activity, while the up-regulation of COX10 increases heme biosynthesis—both impair chlorophyll synthesis. Conversely, the down-regulation of HEMD hindered chlorophyll synthesis, and the up-regulation of SGR enhanced chlorophyll degradation, resulting in reduced chlorophyll content. Additionally, genes linked to carotenoid biosynthesis, flavonoid metabolism, and photosynthesis were significantly down-regulated. We also identified 311 putative differentially expressed transcription factors, including bHLHs and GLKs. These findings shed light on the molecular mechanisms underlying leaf color mutation in I. × attenuata ‘Sunny Foster’ and provide a substantial gene reservoir for enhancing leaf color through breeding techniques. Full article

Wire and arc additive manufacturing (WAAM), recognized for its capability to fabricate large-scale, complex parts, stands out due to its significant deposition rates and cost-effectiveness, positioning it as a forward-looking manufacturing method. In this research, we employed two welding currents to produce samples of 316 austenitic stainless steel utilizing the Cold Metal Transfer wire arc additive manufacturing process (CMT-WAAM). This study initially evaluated the maximum allowable arc travel speed (MAWFS) and the formation characteristics of the deposition bead, considering deposition currents that vary between 100 A and175 A in both CMT and CMT pulse(CMT+P) modes. Thereafter, the effect of the CMT+P mode arc on the microstructure evolution was analyzed using the EBSD technique. The findings indicate that the arc travel speed and deposition current significantly affect the deposition bead’s dimensions. Specifically, an increase in travel speed or a reduction in current results in reduced bead width and height. Moreover, the employment of the CMT+P arc mode led to a reduction in the average grain size in the mid-section of the sample fabricated by CMT arc and wire additive manufacturing, from 13.426 μm to 9.429 μm. Therefore, the components of 316 stainless steel produced through the CMT+P-WAAM method are considered fit for industrial applications. Full article

Cytokines produced by peripheral T-helper 1/17 cells disproportionately contribute to the inflammation (i.e., metaflammation) that fuels type 2 diabetes (T2D) pathogenesis. Shifts in the nutrient milieu could influence inflammation through changes in T-cell metabolism. We aimed to determine whether changes in glucose utilization alter cytokine profiles in T2D. Peripheral blood mononuclear cells (PBMCs), CD4⁺ T-cells, and CD4⁺CD25^- T-effector (T_eff) cells were isolated from age-matched humans classified by glycemic control and BMI. Cytokines secreted by CD3/CD28-stimulated PBMCs and T_eff were measured in supernatants with multiplex cytokine assays and a FLEXMAP-3D. Metabolic activity of stimulated CD4⁺ T-cells was measured by a Seahorse XFe96 analyzer. In this study, we demonstrated that T-cell stimulated PBMCs from non-fasted people with T2D produced higher amounts of cytokines compared to fasting. Although dysglycemia characterizes T2D, cytokine production by PBMCs or CD4⁺ T-cells in T2D was unaltered by hyperglycemic media. Moreover, pharmacological suppression of mitochondrial glucose oxidation did not change T-cell metabolism in T2D, yet enhanced cytokine competency. In conclusion, fasting and glucose metabolism differentially impact peripheral inflammation in human T2D, suggesting that glucose, along with fatty acid metabolites per our previous work, partner to regulate metaflammation. These data expose a major disconnect in the use of glycemic control drugs to target T2D-associated metaflammation. Full article

In the present paper, we address the following general question in the framework of classical first-order logic. Assume that a certain mathematical principle can be formalized in a first-order language by a set E of conditional formulas of the form $\alpha \left(v\right)\to \beta \left(v\right)$. Given a base theory T, we can use the set of conditional formulas E to extend the base theory in two natural ways. Either we add to T each formula in E as a new axiom (thus obtaining a theory denoted by $T+E$) or we extend T by using the formulas in E as instances of an inference rule (thus obtaining a theory denoted by $T+E-\mathrm{Rule}$). The theory $T+E$ will be stronger than $T+E-\mathrm{Rule}$, but how much stronger can $T+E$ be? More specifically, is $T+E$ conservative over $T+E-\mathrm{Rule}$ for theorems of some fixed syntactical complexity $\mathsf{\Gamma }$? Under very general assumptions on the set of conditional formulas E, we obtain two main conservation results in this regard. Firstly, if the formulas in E have low syntactical complexity with respect to some prescribed class of formulas $\mathsf{\Pi }$ and in the applications of $E-\mathrm{Rule}$ side formulas from the class $\mathsf{\Pi }$ and can be eliminated (in a certain precise sense), then $T+E$ is $\forall \mathcal{B}\left(\mathsf{\Pi }\right)$-conservative over $T+E-\mathrm{Rule}$. Secondly, if, in addition, E is a finite set with m conditional sentences, then nested applications of $E-\mathrm{Rule}$ of a depth at most of m suffice to obtain $\forall \mathcal{B}\left(\mathsf{\Pi }\right)$ conservativity. These conservation results between axioms and inference rules extend well-known conservation theorems for fragments of first-order arithmetics to a general, purely logical framework. Full article

Background: The aim of the study was to demonstrate the influence of virtual reality (VR) exposure on postural stability and determine the mechanism of this influence. Methods: Twenty-six male participants aged 21–23 years were included, who underwent postural stability assessment twice before and after a few minute of single VR exposure. The VR projection was a computer-generated simulation of the surrounding scenery. Postural stability was assessed using the Sensory Organization Test (SOT), using Computerized Dynamic Posturography (CDP). Results: The findings indicated that VR exposure affects the visual and vestibular systems. Significant differences (p < 0.05) in results before and after VR exposure were observed in tests on an unstable surface. It was confirmed that VR exposure has a positive influence on postural stability, attributed to an increase in the sensory weight of the vestibular system. Partial evidence suggested that the reduction in vestibulo-ocular reflex (VOR) reinforcement may result in an adaptive shift to the optokinetic reflex (OKR). Conclusions: By modifying the process of environmental perception through artificial sensory simulation, the influence of VR on postural stability has been demonstrated. The validity of this type of research is determined by the effectiveness of VR techniques in the field of vestibular rehabilitation. Full article

In the present study, the hot roll bonding process of 7000 series aluminum alloy laminated materials was numerically simulated and investigated using the finite element method, and the process parameters were experimentally verified by properties testing and microstructure analysis after hot roll bonding. In the roll bonding process of aluminum alloy laminated materials, the effects of the intermediate layer, pass reduction ratio, rolling speed and thickness ratio of component layers were studied. The results of finite element simulations showed that the addition of a 701 intermediate layer in the hot roll bonding process could effectively coordinate the deformation of the 705 layer and 706 layer and prevented the warping of the laminated material during hot rolling. It is recommended to use a multi-pass rolling process with small deformation and high speed, and the recommended rolling reduction ratio is 20%~30%, the hot rolling speed is 1.5~2.5 m/s and the thickness ratio of the 705 layer and 706 layer is about 1:5. Based on the above numerical results, five-layer and seven-layer 7000 series aluminum alloy laminated materials were prepared by the hot roll bonding process. The results showed that metallurgical bonding was realized between each component layer, and no delamination was observed from the tensile fracture between the interfaces of component layers. The tensile strength of the prepared laminated materials decreased with the increase in the thickness ratio of the 705 layer, and the bonding strengths of the laminated materials were in the range of 88–99 MPa. The experimental results verified the rationality of the process parameters recommended by the numerical simulations in terms of warping and delamination prevention. Full article

A large number of research stations have been established to provide members of Antarctic expeditions with logistical support. A previous study confirmed that the wind and solar energy resources of the Chinese Zhongshan Station, a coastal station located in an area of Lassmann Hills in East Antarctica, are highly synergetic and complementary. Considering the demand for a renewable energy power supply in Zhongshan Station, this paper introduces a hybrid energy system with wind–solar–diesel–battery co-generation used as a power supply scheme. Based on the 2015 climate data for Zhongshan Station, the wind–solar resources, conventional energy system, and annual diesel consumption of the station area were analyzed. The annual electrical load demand of each building in the station area was quantitatively expounded. Compared with the original power supply system, and combined with the analysis results of the thermal load and electrical load demands of the station, an objective function based on the requirements of economy, reliability, and environmental protection was presented. According to the constraint conditions of the heat energy and electrical energy load balance in the station area, a multi-objective scheduling strategy for the system was designed. Finally, the effects of this scheduling strategy were analyzed under three different application scenarios. The results indicated that the annual load demand was significantly lower than before the scheduling, and that a 50% reduction in diesel consumption could be achieved, demonstrating that the multi-objective scheduling strategy proposed in this paper could achieve optimal energy scheduling and management of the renewable hybrid energy system. Full article

To enhance the understanding of yield-related traits in tetraploid wheat, it is crucial to investigate and identify genes that govern superior yield characteristics. This study utilized the wheat55K single nucleotide polymorphism array to genotype a recombinant inbred line (RIL) population consisting of 120 lines developed through the crossbreeding of two tetraploid wheat varieties, Qin Hei-1 (QH-1) and Durum Wheat (DW). An investigation and analysis were conducted on 11 yield-related traits, including peduncle length (PL), neck length (NL), spike length (SL), flowering date (FD), heading date (HD), thousand-kernel weight (TKW), kernel area ratio (KAR), kernel circumference (KC), kernel length (KL), kernel width (KW), and kernel length–width ratio (KL-WR), over a period of three years in two locations, Yang Ling, Shaanxi, and Lin He, Inner Mongolia. The analysis identified nine stable loci among eight agronomic traits, named QSL.QD-1A.1, QNL.QD-4B.2, QPL.QD-4B.1, QFD.QD-2B, QHD.QD-2B.1, QHD.QD-4B, QKC.QD-4B.2, QKL-WR.QD-4B.6, and QKL.QD-4B.2. Among them, the additive effects of three QTLs, QSL.QD-1A.1, QNL.QD-4B.2, and QFD.QD-2B, were positive, indicating that the enhancing alleles at these loci were derived from the parent line QH-1. These three QTLs showed significant positive effects on the phenotypes of the population materials. Furthermore, potential functional genes were identified within the mapping intervals of QSL.QD-1A.1 and QNL.QD-4B.2, which regulate the development of spike length and neck length, respectively. These results provide potential QTLs and candidate genes, which broaden the genetic basis of agronomic traits related to yield, such as SL, NL, PL, and FD, and benefits for wheat breeding and improvement. Full article

The Oder River, situated along the border between Poland and Germany, is regularly affected by ice-jam events and their associated hazards, such as a sudden rise in water level and the endangerment to flood-protection infrastructure. The existing databases on past ice-jam events lack substantial information considering ice formation, blockage origins or the spatiotemporal evolution of the ice cover needed for a comprehensive understanding of relevant ice processes. Within this study, the evaluation of satellite and Uncrewed Aerial Vehicle (UAV) data was carried out in order to analyze the capabilities of enhancing river ice information in the study area. Satellite imagery was proven to be a valuable source of investigating ice-jam phenomena on all scales, leading to the identification of initial ice-jam locations, surveying spatiotemporal ice cover evolution or monitoring the maximum ice-cover extent. A simplified approach for river ice classification of satellite radar data using the K-Means Cluster Analysis is introduced, enabling the differentiation between river ice formations. Based on UAV data taken in this study, workflows were presented, allowing for measurements of ice floe velocities and the localization of flooded and ice-covered flow control structures. Full article

The chemical 4-amino-3-nitrophenol (4A3NP) is classified as an amino nitrophenol and is primarily utilized as an ingredient in hair dye colorants. In Korea and Europe, it is exclusively used in non-oxidative or oxidative hair dye formulations, with maximum allowable concentrations of 1% and 1.5%, respectively. Despite this widespread use, risk assessment of 4A3NP has not been completed due to the lack of proper dermal absorption data. Therefore, in this study, both the analytical method validation and in vitro dermal absorption study of 4A3NP were conducted following the guidelines provided by the Korea Ministry of Food and Drug Safety (MFDS). Before proceeding with the dermal absorption study, analytical methods were developed for the quantitation of 4A3NP through multiple reaction monitoring (MRM) via liquid chromatography-mass spectrometry (LC-MS) in various matrices, including swab wash (WASH), stratum corneum (SC), skin (SKIN, comprising the dermis and epidermis), and receptor fluid (RF). These developed methods demonstrated excellent linearity (R² = 0.9962–0.9993), accuracy (93.5–111.73%), and precision (1.7–14.46%) in accordance with the validation guidelines.The dermal absorption of 4A3NP was determined using Franz diffusion cells with mini-pig skin as the barrier. Under both non-oxidative and oxidative (6% hydrogen peroxide (H₂O₂): water, 1:1) hair dye conditions, 1% and 1.5% concentrations of 4A3NP were applied to the skin at a rate of 10 μL/cm², respectively. The total dermal absorption rates of 4A3NP under non-oxidative (1%) and oxidative (1.5%) conditions were determined to be 5.62 ± 2.19% (5.62 ± 2.19 μg/cm²) and 2.83 ± 1.48% (4.24 ± 2.21 μg/cm²), respectively. Full article

Surface treatment technologies are pivotal across diverse industrial sectors such as mechanical engineering, electrical engineering, and the automotive industry. Continuous advancements in manufacturing processes are geared towards bolstering efficiency and attaining superior product quality. This study aimed to empirically compare practical outcomes with theoretical insights. Employing galvanic zinc plating under constant voltage with varying plating durations unveiled a correlation between coating thickness and electrolyte composition alongside plating duration. The graphical representation delineated the optimal electrolyte composition conducive to maximal coating thickness. Notably, an evident decrease in leveling ability was noted with prolonged plating durations. The experiment corroborated the notion that theoretical formulas for coating thickness estimation possess limited accuracy, often resulting in measured values surpassing theoretical predictions. These findings underscore the imperative for refined theoretical models to comprehensively grasp galvanic surface treatment processes. Full article

The anaplastic lymphoma kinase (ALK, CD247) is a potential target for antibody-based therapy. However, no antibody-based therapeutics targeting ALK have entered clinical trials, necessitating the development of novel antibodies with unique therapeutic merits. Single-domain antibodies (sdAb) bear therapeutic advantages compared to the full-length antibody including deeper tumor penetration, cost-effective production and fast washout from normal tissues. In this study, we identified a human immunoglobulin heavy chain variable domain (VH domain) (VH20) from an in-house phage library. VH20 exhibits good developability and high specificity with no off-target binding to ~6000 human membrane proteins. VH20 efficiently bound to the glycine-rich region of ALK with an EC₅₀ of 0.4 nM and a KD of 6.54 nM. Both VH20-based bispecific T cell engager (TCE) and chimeric antigen receptor T cells (CAR Ts) exhibited potent cytolytic activity to ALK-expressing tumor cells in an ALK-dependent manner. VH20 CAR Ts specifically secreted proinflammatory cytokines including IL-2, TNFα and IFNγ after incubation with ALK-positive cells. To our knowledge, this is the first reported human single-domain antibody against ALK. Our in vitro characterization data indicate that VH20 could be a promising ALK-targeting sdAb with potential applications in ALK-expressing tumors, including neuroblastoma (NBL) and non-small cell lung cancer. Full article

Background and Objectives: Assess the quality of life of children aged 2–10 with mild to moderate bronchial asthma. To evaluate the general health condition of children with mild and moderate severity bronchial asthma. To determine health changes in children with mild- and moderate-severity bronchial asthma as they grow older. To evaluate the impact of mild- and moderate-severity bronchial asthma on children’s daily and social activities, physical health, emotional state, and general well-being. Materials and Methods: A comparative cross-sectional study was conducted in March–June 2020. Parents or guardians of 2–10-year-old children without bronchial asthma and children with mild to moderate bronchial asthma were interviewed after receiving their written informed consent. The questionnaire was based on the standardized quality-of-life quiz SF-36. A total of 248 questionnaires were collected—106 from the parents or guardians of children with bronchial asthma and 142 from parents/guardians of children without bronchial asthma. For further analysis, 106 children without bronchial asthma and with no chronic conditions were selected. Quantitative variables were compared using the Mann–Whitney U test and qualitative data using the chi-square (χ²) criteria. Quantitative data were described by giving means, medians, and standard deviations (SD); qualitative features by giving relative frequencies. Statistical data were analyzed using SPSS and Excel 2020. Results: Children with mild and moderate asthma exhibit poorer health compared to their healthy counterparts. Only 20.7% of respondents with asthma reported excellent or very good health, contrasting with 64.1% of healthy children (p < 0.001). As children with asthma age, their general condition improves, with 46.2% showing improvement in the past year, while 42.5% of healthy children had a stable condition (p < 0.05). In various activities, children with asthma face more constraints than healthy children (p < 0.05), including energetic activities (sick—59.5%; healthy—10.3%), moderate activities (sick—24.5%; healthy—4.7%), climbing stairs (sick—22.7%; healthy—3.8%), and walking over 100 m (sick—9.4%; healthy—0%). Children with asthma are more likely to experience exhaustion, anxiety, tiredness, lack of energy, and restraint in public activities (p < 0.05). Conclusions: Parents/caregivers of children with mild to moderate bronchial asthma rate their health worse than those of healthy children do. As children with mild to moderate bronchial asthma grow, the disease impact on their overall well-being decreases. Children with mild to moderate bronchial asthma, compared to healthy children, experience more limitations in vigorous or moderate activities; face more difficulties climbing stairs or walking more than 100 m; frequently feel exhaustion, anxiety, fatigue, or lack of energy; and encounter restrictions in social activities. Full article

The crystal structure of 4-methylcatechol (4MEC) has, to date, never been solved, despite its very simple chemical formula C₇O₂H₈ and the many possible applications envisaged for this molecule. In this work, this gap is filled and the structure of 4MEC is obtained by combining X-ray powder diffraction and first principle calculations to carefully locate hydrogen atoms. Two molecules are present in the asymmetric unit. Hirshfeld analysis confirmed the reliability of the solved structure, since the two molecules show rather different environments and H-bond interactions of different directionality and strength. The packing is characterised by a peculiar hydrogen bond network with hydroxyl nests formed by two adjacent octagonal frameworks. It is noteworthy that the observed short contacts suggest strong inter-molecular interactions, further confirmed by strong inter-crystalline aggregation observed by microscopic images, indicating the growth, in many crystallization attempts, of single aggregates taller than half a centimetre and, often, with spherical shapes. These peculiarities are induced by the presence of methyl group in 4MEC, since the parent compound catechol, despite its chemical similarity, shows a standard layered packing alternating hydrophobic and polar layers. Finally, the complexity and peculiarity of the packing and crystal growth features explain why a single crystal could not be obtained for a standard structural analysis. Full article

In the current study, the effects of drought stress on the growth and phytochemical profile of Scolymus hispanicus L. (a.k.a. golden thistle) were evaluated. Plants were treated with three irrigation regimes, e.g., plants that received only rainwater (Control; C), deficit irrigation (I1; 50% of field capacity (FC)), and full irrigation (Ι2; 100% of FC). The fresh weight of the rosette of leaves was not negatively impacted by deficit irrigation, whereas root development was severely restrained compared to control and I2 treatments. Drought stress conditions had a positive effect on the nutritional properties of the golden thistle since the treatments of control and deficit irrigation showed the highest content of macronutrients and energy. Oxalic acid was the richest organic acid, especially under the I1 regime. Similarly, α-tocopherol was the only identified vitamin E isoform, whose content was also doubled in I1 treatment. Raffinose, glucose, and sucrose were the most abundant free sugars in amounts that varied among the irrigation treatments, while the total and distinct free sugar content was the highest for the I1 treatment. The most abundant detected fatty acid compounds were α-linolenic acid, followed by palmitic and linoleic acid, with the highest amount being detected in C, I1, and I2 treatments, respectively. Flavonoids were the only class of polyphenols detected in golden thistle leaves, including mostly kaempferol and quercetin derivatives. The greatest antioxidant potency was shown for the control and I1 treatments (for OxHLIA and TBARS methods, respectively). The evaluated leaf samples recorded a varied antimicrobial effect for the different bacterial strains and fungi, whereas no cytotoxic, hepatotoxic, and anti-inflammatory effects against the tested cell lines were recorded. Finally, the mineral content of leaves was significantly affected by the irrigation regime, with Ca, Mg, Cu, and Zn being the highest for the I1 treatment, while the I2 treatment had the highest content of K, Fe, and Mn and the lowest Na content. In conclusion, deficit irrigation showed promising results since it improved the phytochemical content without compromising the fresh weight of leaves, and thus it could be suggested as a sustainable agronomic practice for producing high-added value products without significant constraints in growth development and yield parameters of golden thistle. Full article