The 2023 MDPI Annual Report has
been released!
 
25 pages, 9166 KiB  
Article
Molecular Mechanism of Phosphorylation-Mediated Impacts on the Conformation Dynamics of GTP-Bound KRAS Probed by GaMD Trajectory-Based Deep Learning
by Jianzhong Chen, Jian Wang, Wanchun Yang, Lu Zhao, Juan Zhao and Guodong Hu
Molecules 2024, 29(10), 2317; https://doi.org/10.3390/molecules29102317 (registering DOI) - 15 May 2024
Abstract
The phosphorylation of different sites produces a significant effect on the conformational dynamics of KRAS. Gaussian accelerated molecular dynamics (GaMD) simulations were combined with deep learning (DL) to explore the molecular mechanism of the phosphorylation-mediated effect on conformational dynamics of the GTP-bound KRAS. [...] Read more.
The phosphorylation of different sites produces a significant effect on the conformational dynamics of KRAS. Gaussian accelerated molecular dynamics (GaMD) simulations were combined with deep learning (DL) to explore the molecular mechanism of the phosphorylation-mediated effect on conformational dynamics of the GTP-bound KRAS. The DL finds that the switch domains are involved in obvious differences in conformation contacts and suggests that the switch domains play a key role in the function of KRAS. The analyses of free energy landscapes (FELs) reveal that the phosphorylation of pY32, pY64, and pY137 leads to more disordered states of the switch domains than the wild-type (WT) KRAS and induces conformational transformations between the closed and open states. The results from principal component analysis (PCA) indicate that principal motions PC1 and PC2 are responsible for the closed and open states of the phosphorylated KRAS. Interaction networks were analyzed and the results verify that the phosphorylation alters interactions of GTP and magnesium ion Mg2+ with the switch domains. It is concluded that the phosphorylation pY32, pY64, and pY137 tune the activity of KRAS through changing conformational dynamics and interactions of the switch domains. We anticipated that this work could provide theoretical aids for deeply understanding the function of KRAS. Full article
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18 pages, 10156 KiB  
Article
Reinforcement of DC Electrified Railways by a Modular Battery Energy Storage System
by Erick Matheus da Silveira Brito, Philippe Ladoux, Joseph Fabre and Benoit Sonier
Electronics 2024, 13(10), 1933; https://doi.org/10.3390/electronics13101933 (registering DOI) - 15 May 2024
Abstract
DC railway electrification was deployed at the beginning of the 20th century in several countries in Europe. Today, this power system is no longer adapted to the demands of increased rail traffic. Due to the relatively low voltage level, the current consumed by [...] Read more.
DC railway electrification was deployed at the beginning of the 20th century in several countries in Europe. Today, this power system is no longer adapted to the demands of increased rail traffic. Due to the relatively low voltage level, the current consumed by the trains reaches several kAs. So, in the worst case, the locomotives cannot operate at their rated power due to the voltage drop along the contact line. Conventional solutions to reduce the voltage drop consist of increasing the cross-section of overhead lines or reducing the length of sectors by installing additional substations. Nevertheless, these solutions are expensive and not always feasible. The implementation of a Modular Battery Energy Storage System (MBESS) can be an alternative solution to reinforce the railway power supply. This paper first presents an MBESS based on elementary blocks associating Full-SiC Isolated DC-DC converter and battery racks. The electrical models of a railway sector and an elementary block are described, and simulations are performed considering real railroad traffic on two sectors of the French National Rail Network, electrified at 1.5 kV. The results show that the installation of an MBESS in the railway sector boosts the locomotive’s voltage while also increasing overall system efficiency. Full article
(This article belongs to the Special Issue Railway Traction Power Supply, 2nd Edition)
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19 pages, 4804 KiB  
Article
Mapping Bovine Tuberculosis in Colombia, 2001–2019
by D. Katterine Bonilla-Aldana, S. Daniela Jiménez-Diaz, Carlos Lozada-Riascos, Kenneth Silva-Cajaleon and Alfonso J. Rodríguez-Morales
Vet. Sci. 2024, 11(5), 220; https://doi.org/10.3390/vetsci11050220 (registering DOI) - 15 May 2024
Abstract
Introduction: Bovine tuberculosis is a zoonotic disease of significant impact, particularly in countries where a pastoral economy is predominant. Despite its importance, few studies have analysed the disease’s behaviour in Colombia, and none have developed maps using geographic information systems (GIS) to characterise [...] Read more.
Introduction: Bovine tuberculosis is a zoonotic disease of significant impact, particularly in countries where a pastoral economy is predominant. Despite its importance, few studies have analysed the disease’s behaviour in Colombia, and none have developed maps using geographic information systems (GIS) to characterise it; as such, we developed this study to describe the temporal–spatial distribution of bovine tuberculosis in Colombia over a period of 19 years. Methods: A retrospective cross-sectional descriptive study, based on reports by the Colombian Agricultural Institute (ICA), surveillance of tuberculosis on cattle farms in Colombia from 2001 to 2019 was carried out. The data were converted into databases using Microsoft Access 365®, and multiple epidemiological maps were generated with the QGIS® version 3.36 software coupled to shape files of all the country’s departments. Results: During the study period, 5273 bovine tuberculosis cases were identified in multiple different departments of Colombia (with a mean of 278 cases/year). Regarding its temporal distribution, the number of cases varied from a maximum of 903 cases (17.12% of the total) in 2015 to a minimum of 0 between 2001 and 2004 and between 2017 and 2019 (between 2005 and 2016, the minimum was 46 cases, 0.87%). Conclusions: GIS are essential for understanding the temporospatial behaviour of zoonotic diseases in Colombia, as is the case for bovine tuberculosis, with its potential implications for the Human and One Health approaches. Full article
(This article belongs to the Special Issue Spotlight on Tuberculosis and Mycoplasmosis in Cattle)
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14 pages, 2979 KiB  
Article
Tumor-Intrinsic Enhancer of Zeste Homolog 2 Controls Immune Cell Infiltration, Tumor Growth, and Lung Metastasis in a Triple-Negative Breast Cancer Model
by Lenore Monterroza, Maria M. Parrilla, Sarah G. Samaranayake, Dormarie E. Rivera-Rodriguez, Sung Bo Yoon, Ramireddy Bommireddy, Justin Hosten, Luisa Cervantes Barragan, Adam Marcus, Brian S. Dobosh, Periasamy Selvaraj and Rabindra Tirouvanziam
Int. J. Mol. Sci. 2024, 25(10), 5392; https://doi.org/10.3390/ijms25105392 (registering DOI) - 15 May 2024
Abstract
Triple-negative breast cancer (TNBC) is an aggressive and highly metastatic type of tumor. TNBC is often enriched in tumor-infiltrating neutrophils (TINs), which support cancer growth in part by counteracting tumor-infiltrating lymphocytes (TILs). Prior studies identified the enhancer of zeste homolog 2 (EZH2) as [...] Read more.
Triple-negative breast cancer (TNBC) is an aggressive and highly metastatic type of tumor. TNBC is often enriched in tumor-infiltrating neutrophils (TINs), which support cancer growth in part by counteracting tumor-infiltrating lymphocytes (TILs). Prior studies identified the enhancer of zeste homolog 2 (EZH2) as a pro-tumor methyltransferase in primary and metastatic TNBCs. We hypothesized that EZH2 inhibition in TNBC cells per se would exert antitumor activity by altering the tumor immune microenvironment. To test this hypothesis, we used CRISPR to generate EZH2 gene knockout (KO) and overexpressing (OE) lines from parent (wild-type—WT) 4T1 cells, an established murine TNBC model, resulting in EZH2 protein KO and OE, respectively. In vitro, EZH2 KO and OE cells showed early, transient changes in replicative capacity and invasiveness, and marked changes in surface marker profile and cytokine/chemokine secretion compared to WT cells. In vivo, EZH2 KO cells showed significantly reduced primary tumor growth and a 10-fold decrease in lung metastasis compared to WT cells, while EZH2 OE cells were unchanged. Compared to WT tumors, TIN:TIL ratios were greatly reduced in EZH2 KO tumors but unchanged in EZH2 OE tumors. Thus, EZH2 is key to 4T1 aggressiveness as its tumor-intrinsic knockout alters their in vitro secretome and in vivo primary tumor growth, TIN/TIL poise, and metastasis. Full article
(This article belongs to the Special Issue Advances and Mechanisms in Breast Cancer)
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5 pages, 181 KiB  
Book Review
Book Review: Powdyel, T.S. My Green School: An Outline; 2020; ISBN-13: 979-8677417979
by Anh-Tuan Hoang and Tzu-Bin Lin
Sustainability 2024, 16(10), 4131; https://doi.org/10.3390/su16104131 (registering DOI) - 15 May 2024
Abstract
The Green School—An outline reflects on the author’s vision of a holistic approach to the education system as a leading arrow for human beings and social prosperity. It is a needed book, an education of choice, conviction, and passion by Thakur S. Powdyel, [...] Read more.
The Green School—An outline reflects on the author’s vision of a holistic approach to the education system as a leading arrow for human beings and social prosperity. It is a needed book, an education of choice, conviction, and passion by Thakur S. Powdyel, former Minister of Education, Royal Government of Bhutan a long with several studies in terms of reflections on Gross National Happiness in the education context and globalization, and context of development. Thus, Powdyel’s Green School conducts all the fundamentals as well as many other crucial aspects related to greenery concepts in the entire school as a whole-green school approach for future education. Full article
26 pages, 8045 KiB  
Article
Improving Wheat Leaf Nitrogen Concentration (LNC) Estimation across Multiple Growth Stages Using Feature Combination Indices (FCIs) from UAV Multispectral Imagery
by Xiangxiang Su, Ying Nian, Hu Yue, Yongji Zhu, Jun Li, Weiqiang Wang, Yali Sheng, Qiang Ma, Jikai Liu, Wenhui Wang and Xinwei Li
Agronomy 2024, 14(5), 1052; https://doi.org/10.3390/agronomy14051052 (registering DOI) - 15 May 2024
Abstract
Leaf nitrogen concentration (LNC) is a primary indicator of crop nitrogen status, closely related to the growth and development dynamics of crops. Accurate and efficient monitoring of LNC is significant for precision field crop management and enhancing crop productivity. However, the biochemical properties [...] Read more.
Leaf nitrogen concentration (LNC) is a primary indicator of crop nitrogen status, closely related to the growth and development dynamics of crops. Accurate and efficient monitoring of LNC is significant for precision field crop management and enhancing crop productivity. However, the biochemical properties and canopy structure of wheat change across different growth stages, leading to variations in spectral responses that significantly impact the estimation of wheat LNC. This study aims to investigate the construction of feature combination indices (FCIs) sensitive to LNC across multiple wheat growth stages, using remote sensing data to develop an LNC estimation model that is suitable for multiple growth stages. The research employs UAV multispectral remote sensing technology to acquire canopy imagery of wheat during the early (Jointing stage and Booting stage) and late (Early filling and Late filling stages) in 2021 and 2022, extracting spectral band reflectance and texture metrics. Initially, twelve sensitive spectral feature combination indices (SFCIs) were constructed using spectral band information. Subsequently, sensitive texture feature combination indices (TFCIs) were created using texture metrics as an alternative to spectral bands. Machine learning algorithms, including partial least squares regression (PLSR), random forest regression (RFR), support vector regression (SVR), and Gaussian process regression (GPR), were used to integrate spectral and texture information, enhancing the estimation performance of wheat LNC across growth stages. Results show that the combination of Red, Red edge, and Near-infrared bands, along with texture metrics such as Mean, Correlation, Contrast, and Dissimilarity, has significant potential for LNC estimation. The constructed SFCIs and TFCIs both enhanced the responsiveness to LNC across multiple growth stages. Additionally, a sensitive index, the Modified Vegetation Index (MVI), demonstrated significant improvement over NDVI, correcting the over-saturation concerns of NDVI in time-series analysis and displaying outstanding potential for LNC estimation. Spectral information outperforms texture information in estimation capability, and their integration, particularly with SVR, achieves the highest precision (coefficient of determination (R2) = 0.786, root mean square error (RMSE) = 0.589%, and relative prediction deviation (RPD) = 2.162). In conclusion, the sensitive FCIs developed in this study improve LNC estimation performance across multiple growth stages, enabling precise monitoring of wheat LNC. This research provides insights and technical support for the construction of sensitive indices and the precise management of nitrogen nutrition status in field crops. Full article
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19 pages, 4565 KiB  
Article
Novel Acetamide-Based HO-1 Inhibitor Counteracts Glioblastoma Progression by Interfering with the Hypoxic–Angiogenic Pathway
by Agata Grazia D’Amico, Grazia Maugeri, Luca Vanella, Valeria Consoli, Valeria Sorrenti, Francesca Bruno, Concetta Federico, Antonino Nicolò Fallica, Valeria Pittalà and Velia D’Agata
Int. J. Mol. Sci. 2024, 25(10), 5389; https://doi.org/10.3390/ijms25105389 (registering DOI) - 15 May 2024
Abstract
Glioblastoma multiforme (GBM) represents the deadliest tumor among brain cancers. It is a solid tumor characterized by uncontrolled cell proliferation generating the hypoxic niches in the cancer core. By inducing the transcription of hypoxic inducible factor (HIF), hypoxia triggers many signaling cascades responsible [...] Read more.
Glioblastoma multiforme (GBM) represents the deadliest tumor among brain cancers. It is a solid tumor characterized by uncontrolled cell proliferation generating the hypoxic niches in the cancer core. By inducing the transcription of hypoxic inducible factor (HIF), hypoxia triggers many signaling cascades responsible for cancer progression and aggressiveness, including enhanced expression of vascular endothelial growth factor (VEGF) or antioxidant enzymes, such as heme oxygenase-1 (HO-1). The present work aimed to investigate the link between HO-1 expression and the hypoxic microenvironment of GBM by culturing two human glioblastoma cell lines (U87MG and A172) in the presence of a hypoxic mimetic agent, deferoxamine (DFX). By targeting hypoxia-induced HO-1, we have tested the effect of a novel acetamide-based HO-1 inhibitor (VP18/58) on GBM progression. Results have demonstrated that hypoxic conditions induced upregulation and nuclear expression of HO-1 in a cell-dependent manner related to malignant phenotype. Moreover, our data demonstrated that the HO-1 inhibitor counteracted GBM progression by modulating the HIFα/HO-1/VEGF signaling cascade in cancer cells bearing more malignant phenotypes. Full article
(This article belongs to the Special Issue Molecular Progression of Genome-Related Diseases)
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17 pages, 4692 KiB  
Article
Genomic and Transcriptome Analysis Reveals the Biosynthesis Network of Cordycepin in Cordyceps militaris
by Linshan Chai, Jianmei Li, Lingling Guo, Shuyu Zhang, Fei Chen, Wanqin Zhu and Yu Li
Genes 2024, 15(5), 626; https://doi.org/10.3390/genes15050626 (registering DOI) - 15 May 2024
Abstract
Cordycepin is the primary active compound of Cordyceps militaris. However, the definitive genetic mechanism governing cordycepin synthesis in fruiting body growth and development remains elusive, necessitating further investigation. This study consists of 64 C. militaris strains collected from northeast China. The high-yielding [...] Read more.
Cordycepin is the primary active compound of Cordyceps militaris. However, the definitive genetic mechanism governing cordycepin synthesis in fruiting body growth and development remains elusive, necessitating further investigation. This study consists of 64 C. militaris strains collected from northeast China. The high-yielding cordycepin strain CMS19 was selected for the analysis of cordycepin production and the genetic basis of cordycepin anabolism. First, the whole-genome sequencing of CMS19 yielded a final size of 30.96 Mb with 8 contigs and 9781 protein-coding genes. The genome component revealed the presence of four additional secondary metabolite gene clusters compared with other published genomes, suggesting the potential for the production of new natural products. The analyses of evolutionary and genetic differentiation revealed a close relationship between C. militaris and Beauveria bassiana. The population of strains distributed in northeast China exhibited the significant genetic variation. Finally, functional genes associated with cordycepin synthesis were identified using a combination of genomic and transcriptomic analyses. A large number of functional genes associated with energy and purine metabolism were significantly enriched, facilitating the reconstruction of a hypothetical cordycepin metabolic pathway. Therefore, our speculation of the cordycepin metabolism pathway involved 24 genes initiating from the glycolysis and pentose phosphate pathways, progressing through purine metabolism, and culminating in the core region of cordycepin synthesis. These findings could offer fundamental support for scientific utilizations of C. militaris germplasm resources and standardized cultivation for cordycepin production. Full article
(This article belongs to the Section Plant Genetics and Genomics)
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20 pages, 739 KiB  
Review
Research Progress in the Separation of Chemical Components from Essential Oils by High-Speed Countercurrent Chromatography
by Linhong He, Zihao Zhong, Lijuan Zhang and Xi Bai
Separations 2024, 11(5), 152; https://doi.org/10.3390/separations11050152 (registering DOI) - 15 May 2024
Abstract
Essential oils (EOs) are vital secondary metabolites in plants. They have garnered substantial attention owing to their distinct flavors and desirable attributes, including potent antioxidant, antibacterial, and antitumor properties. Nevertheless, the active constituents of EOs exhibit intricate chemical structures, and conventional separation techniques [...] Read more.
Essential oils (EOs) are vital secondary metabolites in plants. They have garnered substantial attention owing to their distinct flavors and desirable attributes, including potent antioxidant, antibacterial, and antitumor properties. Nevertheless, the active constituents of EOs exhibit intricate chemical structures, and conventional separation techniques are inadequate for purifying the individual chemical components from EOs. High-speed countercurrent chromatography, based on the principles of a hydrodynamic equilibrium system, has emerged as a liquid–liquid chromatographic separation method renowned for its ability to handle substantial single injection volumes and the absence of irreversible adsorption. Consequently, in recent years, this technique has been widely employed in the isolation and refinement of natural products. In this review, a comprehensive analysis is conducted, contrasting the merits and demerits of high-speed countercurrent chromatography with conventional separation methods. The solvent systems, elution modes, commonly employed detectors, and practical applications are reviewed in the context of high-speed countercurrent chromatography for essential oil separation and purification. Furthermore, this review offers a glimpse into the potential prospects of applying this technique, with the intention of serving as a valuable reference for the use of high-speed countercurrent chromatography in the purification of EOs. Full article
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24 pages, 4955 KiB  
Article
Phenomenology of the Composition of PM2.5 at an Urban Site in Northern France
by Yamina Allouche, Marc Fadel, Amélie Ferté, Anthony Verdin, Frédéric Ledoux and Dominique Courcot
Atmosphere 2024, 15(5), 603; https://doi.org/10.3390/atmos15050603 (registering DOI) - 15 May 2024
Abstract
In this work, PM2.5 was sampled at Dunkerque, a medium-sized city located in northern France. The mean concentration of PM2.5 during the sampling period was 12.6 ± 9.5 μg·m−3. Samples were analyzed for elemental and organic carbon (EC/OC), water-soluble [...] Read more.
In this work, PM2.5 was sampled at Dunkerque, a medium-sized city located in northern France. The mean concentration of PM2.5 during the sampling period was 12.6 ± 9.5 μg·m−3. Samples were analyzed for elemental and organic carbon (EC/OC), water-soluble organic carbon (WSOC), humic-like substances (HULIS-C), water-soluble inorganic ions, and major and trace elements. The origin and the variations of species concentrations were examined using elemental enrichment factors, bivariate polar plot representations, and diagnostic concentration ratios. Secondary inorganic ions were the most abundant species (36% of PM2.5), followed by OC (12.5% of PM2.5). Secondary organic carbon (SOC) concentrations were estimated to account for 52% of OC. A good correlation between SOC and WSOC indicated that secondary formation processes significantly contribute to the WSOC concentrations. HULIS-C also represents almost 50% of WSOC. The determination of diagnostic ratios revealed the influence of anthropogenic emission sources such as integrated steelworks and fuel oil combustion. The clustering of 72 h air masses backward trajectories data evidenced that higher concentrations of PM2.5, OC, and secondary inorganic aerosols were recorded when air masses came from north-eastern Europe and the French continental sector, showing the considerable impact of long-range transport on the air quality in northern France. Full article
(This article belongs to the Special Issue Characteristics and Source Apportionment of Urban Air Pollution)
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15 pages, 9807 KiB  
Article
Investigation of the Tribological Properties of Hybrid Additive-Modified Water-Based Lubricating Fluid
by Raimondas Kreivaitis, Jolanta Treinytė, Artūras Kupčinskas, Milda Gumbytė and Eglė Sendžikienė
Lubricants 2024, 12(5), 178; https://doi.org/10.3390/lubricants12050178 (registering DOI) - 15 May 2024
Abstract
Water-based lubricating fluids (WBLFs), known for their significant environmental benefits, are the focus of this study. The properties of WBLFs directly influence lubricated mechanisms’ longevity and operating efficiency. WBLFs are enhanced using additives, which must improve their properties and, at the same time, [...] Read more.
Water-based lubricating fluids (WBLFs), known for their significant environmental benefits, are the focus of this study. The properties of WBLFs directly influence lubricated mechanisms’ longevity and operating efficiency. WBLFs are enhanced using additives, which must improve their properties and, at the same time, remain environmentally friendly. This study combines bis(2-hydroxyethyl) ammonium erucate protic ionic liquid and titanium oxide nanoparticles to formulate the hybrid additive. The lubricity was investigated using Alumina/Bearing steel and WC/Bearing steel friction pairs in a reciprocating ball-on-plate tribo-tester. The results show that protic ionic liquid can significantly improve lubricity and the corrosion-preventing ability of the base fluid. Applying a hybrid additive further improved the wear reduction ability in the WC/Bearing steel friction pair. However, the wear reduction ability was diminished when a hybrid additive was used to lubricate the Alumina/Bearing steel friction pair. The proposed lubricity improvement mechanism is based on forming an adsorption layer of ionic liquid molecules and rolling and tribo-sintering titanium oxide nanoparticles. Full article
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13 pages, 6417 KiB  
Article
Preparation and Application of High Internal Phase Pickering Emulsion Gels Stabilized by Starch Nanocrystal/Tannic Acid Complex Particles
by Haoran Jin, Chen Li, Yajuan Sun, Bingtian Zhao and Yunxing Li
Gels 2024, 10(5), 335; https://doi.org/10.3390/gels10050335 (registering DOI) - 15 May 2024
Abstract
Herein, the starch nanocrystal/tannic acid (ST) complex particles, which were prepared based on the hydrogen bond between starch nanocrystal (SNC) and tannic acid (TA), were successfully used to stabilize the HIPPE gels. The optimal TA concentration of the ST complex particles resulted in [...] Read more.
Herein, the starch nanocrystal/tannic acid (ST) complex particles, which were prepared based on the hydrogen bond between starch nanocrystal (SNC) and tannic acid (TA), were successfully used to stabilize the HIPPE gels. The optimal TA concentration of the ST complex particles resulted in better water dispersibility, surface wettability, and interfacial activity as compared to SNC. The hydrogen bond responsible for the formation of ST complex particles and subsequent stable emulsions was demonstrated by varying the pH and ionic strength of the aqueous phase. Notably, the HIPPE gels stabilized via the ST complex particles can maintain long-term stability for up to three months. The HIPPEs stabilized via the ST complex particles all displayed gel-like features and had smaller droplets and denser droplet networks than the SNC-stabilized HIPPEs. The rheological behavior of HIPPE gels stabilized via the ST complex particles can be readily changed by tuning the mass ratio of SNC and TA as well as pH. Finally, the prepared HIPPE gels used to effectively protect encapsulated β-carotene against high temperatures and ultraviolet radiation and its controllable release at room temperature were demonstrated. It is anticipated that the aforementioned findings will provide new perspectives on the preparation of Pickering emulsion for delivery systems. Full article
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19 pages, 4186 KiB  
Article
Experimental Study on Evaporation and Micro-Explosion Characteristics of Ethanol and Diesel Blended Droplets
by Yixuan Zhang, Kesheng Meng, Lin Bao, Qizhao Lin and Svitlana Pavlova
Atmosphere 2024, 15(5), 604; https://doi.org/10.3390/atmos15050604 (registering DOI) - 15 May 2024
Abstract
In this study, the constant temperature control system of a heating plate was established, ethanol–diesel fuel with different proportions was prepared, and a series of experiments were carried out. The experimental system was used to observe, summarize, and analyze four evaporation and crushing [...] Read more.
In this study, the constant temperature control system of a heating plate was established, ethanol–diesel fuel with different proportions was prepared, and a series of experiments were carried out. The experimental system was used to observe, summarize, and analyze four evaporation and crushing modes of mixed droplets, which were explosion, liquid filament stretching, exocytosis, and ejection mode. The evaporation process of four kinds of mixed droplets in their life cycle was analyzed by normalizing the diameter square. It was proposed that the evaporation process of droplets could be divided into the following three stages: a heating stage, a fluctuating evaporation stage, and an equilibrium evaporation stage. It was also pointed out that the expansion, ejection, and micro-explosion of droplets were the causes of fluctuating evaporation. The concept of expansion and crushing intensity was put forward and the expansion and crushing intensity of ethanol/diesel mixed droplets with different proportions were calculated. The reasons why expansion and crushing intensity first increased and decreased with the increase in ethanol blending ratio were analyzed. Finally, the time proportion of ethanol–diesel mixed droplets in each evaporation stage was calculated, which explained that the time proportion of the instantaneous heating stage showed a parabolic law with the increase in ethanol content. Full article
(This article belongs to the Special Issue Engine Emissions: Assessment and Control)
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11 pages, 2004 KiB  
Article
Nobiletin Stimulates Adrenal Hormones and Modulates the Circadian Clock in Mice
by Conn Ryan, Yu Tahara, Atsushi Haraguchi, Yuanyuan Lu and Shigenobu Shibata
Nutrients 2024, 16(10), 1491; https://doi.org/10.3390/nu16101491 (registering DOI) - 15 May 2024
Abstract
Polymethoxyflavonoids, such as nobiletin (abundant in Citrus depressa), have been reported to have antioxidant, anti-inflammatory, anticancer, and anti-dementia effects, and are also a circadian clock modulator through retinoic acid receptor-related orphan receptor (ROR) α/γ. However, the optimal timing of nobiletin intake has not [...] Read more.
Polymethoxyflavonoids, such as nobiletin (abundant in Citrus depressa), have been reported to have antioxidant, anti-inflammatory, anticancer, and anti-dementia effects, and are also a circadian clock modulator through retinoic acid receptor-related orphan receptor (ROR) α/γ. However, the optimal timing of nobiletin intake has not yet been determined. Here, we explored the time-dependent treatment effects of nobiletin and a possible novel mechanistic idea for nobiletin-induced circadian clock regulation in mice. In vivo imaging showed that the PER2::LUC rhythm in the peripheral organs was altered in accordance with the timing of nobiletin administration (100 mg/kg). Administration at ZT4 (middle of the light period) caused an advance in the peripheral clock, whereas administration at ZT16 (middle of the dark period) caused an increase in amplitude. In addition, the intraperitoneal injection of nobiletin significantly and potently stimulated corticosterone and adrenaline secretion and caused an increase in Per1 expression in the peripheral tissues. Nobiletin inhibited phosphodiesterase (PDE) 4A1A, 4B1, and 10A2. Nobiletin or rolipram (PDE4 inhibitor) injection, but not SR1078 (RORα/γ agonist), caused acute Per1 expression in the peripheral tissues. Thus, the present study demonstrated a novel function of nobiletin and the regulation of the peripheral circadian clock. Full article
(This article belongs to the Section Micronutrients and Human Health)
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19 pages, 2300 KiB  
Review
The Stria Vascularis: Renewed Attention on a Key Player in Age-Related Hearing Loss
by Sonny Bovee, Georg M. Klump, Christine Köppl and Sonja J. Pyott
Int. J. Mol. Sci. 2024, 25(10), 5391; https://doi.org/10.3390/ijms25105391 (registering DOI) - 15 May 2024
Abstract
Age-related hearing loss (HL), or presbycusis, is a complex and heterogeneous condition, affecting a significant portion of older adults and involving various interacting mechanisms. Metabolic presbycusis, a type of age-related HL, is characterized by the dysfunction of the stria vascularis, which is crucial [...] Read more.
Age-related hearing loss (HL), or presbycusis, is a complex and heterogeneous condition, affecting a significant portion of older adults and involving various interacting mechanisms. Metabolic presbycusis, a type of age-related HL, is characterized by the dysfunction of the stria vascularis, which is crucial for maintaining the endocochlear potential necessary for hearing. Although attention on metabolic presbycusis has waned in recent years, research continues to identify strial pathology as a key factor in age-related HL. This narrative review integrates past and recent research, bridging findings from animal models and human studies, to examine the contributions of the stria vascularis to age-related HL. It provides a brief overview of the structure and function of the stria vascularis and then examines mechanisms contributing to age-related strial dysfunction, including altered ion transport, changes in pigmentation, inflammatory responses, and vascular atrophy. Importantly, this review outlines the contribution of metabolic mechanisms to age-related HL, highlighting areas for future research. It emphasizes the complex interdependence of metabolic and sensorineural mechanisms in the pathology of age-related HL and highlights the importance of animal models in understanding the underlying mechanisms. The comprehensive and mechanistic investigation of all factors contributing to age-related HL, including cochlear metabolic dysfunction, remains crucial to identifying the underlying mechanisms and developing personalized, protective, and restorative treatments. Full article
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12 pages, 2987 KiB  
Article
Hourglass Magnetic Field of a Protostellar System
by Shantanu Basu, Xiyuan Li and Gianfranco Bino
Universe 2024, 10(5), 218; https://doi.org/10.3390/universe10050218 (registering DOI) - 15 May 2024
Abstract
An hourglass-shaped magnetic field pattern arises naturally from the gravitational collapse of a star-forming gas cloud. Most studies have focused on the prestellar collapse phase, when the structure has a smooth and monotonic radial profile. However, most observations target dense clouds that already [...] Read more.
An hourglass-shaped magnetic field pattern arises naturally from the gravitational collapse of a star-forming gas cloud. Most studies have focused on the prestellar collapse phase, when the structure has a smooth and monotonic radial profile. However, most observations target dense clouds that already contain a central protostar, and possibly a circumstellar disk. We utilize an analytic treatment of the magnetic field along with insights gained from simulations to develop a more realistic magnetic field model for the protostellar phase. Key elements of the model are a strong radial magnetic field in the region of rapid collapse, an off-center peak in the magnetic field strength (a consequence of magnetic field dissipation in the circumstellar disk), and a strong toroidal field that is generated in the region of rapid collapse and outflow generation. A model with a highly pinched and twisted magnetic field pattern in the inner collapse zone facilitates the interpretation of magnetic field patterns observed in protostellar clouds. Full article
(This article belongs to the Special Issue Advances in Star Formation in the Milky Way)
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18 pages, 342 KiB  
Article
Effects of the Addition of Trichoderma reesei Cellulase to Broiler Chicken Diets for a 21-Day Period
by Francisco dos Santos Perim, Weslane Justina da Silva, Dênia Oliveira de Souza, Cirano José Ulhoa, Camila Ferreira Rezende, Ludmilla Faria dos Santos, Fabiana Ramos dos Santos, Fabiano Guimarães Silva and Cibele Silva Minafra
Animals 2024, 14(10), 1467; https://doi.org/10.3390/ani14101467 (registering DOI) - 15 May 2024
Abstract
The cellulose present in the cell wall of vegetables prevents the greater release of nutrients to the animal. Therefore, the use of the cellulase enzyme is a viable strategy as it is capable of breaking cellulose bonds, releasing nutrients such as glucose, increasing [...] Read more.
The cellulose present in the cell wall of vegetables prevents the greater release of nutrients to the animal. Therefore, the use of the cellulase enzyme is a viable strategy as it is capable of breaking cellulose bonds, releasing nutrients such as glucose, increasing dietary energy, and thus improving the productive performance of birds. Trichoderma reesei is efficient in the production of cellulase, which is produced via submerged fermentation followed by purification, formulation, and drying. Therefore, an experiment was carried out using 240 male broilers of the Cobb-500® lineage to verify the effects resulting from the addition of powdered (500 g/t and 1000 g/t) and liquid (500 mL/t) cellulase over a period of 1 to 21 days. A completely randomized experimental design was used, consisting of four treatments with six replications and ten birds per replication that were housed in an experimental cage. It was observed that performance and digestibility results were significantly different with cellulase supplementation. Also, the relative weight of the large intestine in the period between one and seven days increased when cellulase was added at 1000 g/t. In the period of between eight and 14 days of life, the birds that consumed only the basal diet obtained higher levels of liver protein than those that received the treatments with the addition of the enzyme. However, 15 and 21 days, the consumed feed effect did not occur between thus, it is not conclusive whether hepatotoxicity occurs with the addition of cellulase. For the blood parameters, at 21 days, the diets with added cellulase were not significantly different regarding electrolytes. It was concluded that this cellulase produced by Trichoderma reesei can be included in the animals’ diet. Full article
(This article belongs to the Collection Sustainable Animal Nutrition and Feeding)
24 pages, 10127 KiB  
Article
Unveiling AI-Generated Financial Text: A Computational Approach Using Natural Language Processing and Generative Artificial Intelligence
by Muhammad Asad Arshed, Ștefan Cristian Gherghina, Christine Dewi, Asma Iqbal and Shahzad Mumtaz
Computation 2024, 12(5), 101; https://doi.org/10.3390/computation12050101 (registering DOI) - 15 May 2024
Abstract
This study is an in-depth exploration of the nascent field of Natural Language Processing (NLP) and generative Artificial Intelligence (AI), and it concentrates on the vital task of distinguishing between human-generated text and content that has been produced by AI models. Particularly, this [...] Read more.
This study is an in-depth exploration of the nascent field of Natural Language Processing (NLP) and generative Artificial Intelligence (AI), and it concentrates on the vital task of distinguishing between human-generated text and content that has been produced by AI models. Particularly, this research pioneers the identification of financial text derived from AI models such as ChatGPT and paraphrasing tools like QuillBot. While our primary focus is on financial content, we have also pinpointed texts generated by paragraph rewriting tools and utilized ChatGPT for various contexts this multiclass identification was missing in previous studies. In this paper, we use a comprehensive feature extraction methodology that combines TF–IDF with Word2Vec, along with individual feature extraction methods. Importantly, combining a Random Forest model with Word2Vec results in impressive outcomes. Moreover, this study investigates the significance of the window size parameters in the Word2Vec approach, revealing that a window size of one produces outstanding scores across various metrics, including accuracy, precision, recall and the F1 measure, all reaching a notable value of 0.74. In addition to this, our developed model performs well in classification, attaining AUC values of 0.94 for the ‘GPT’ class; 0.77 for the ‘Quil’ class; and 0.89 for the ‘Real’ class. We also achieved an accuracy of 0.72, precision of 0.71, recall of 0.72, and F1 of 0.71 for our extended prepared dataset. This study contributes significantly to the evolving landscape of AI text identification, providing valuable insights and promising directions for future research. Full article
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23 pages, 11650 KiB  
Article
Sweeteners Show a Plasticizing Effect on PVP K30—A Solution for the Hot-Melt Extrusion of Fixed-Dose Amorphous Curcumin-Hesperetin Solid Dispersions
by Kamil Wdowiak, Lidia Tajber, Andrzej Miklaszewski and Judyta Cielecka-Piontek
Pharmaceutics 2024, 16(5), 659; https://doi.org/10.3390/pharmaceutics16050659 (registering DOI) - 15 May 2024
Abstract
The co-administration of curcumin and hesperetin might be beneficial in terms of neuroprotective activity; therefore, in this study, we attempted to develop a fixed-dose formulation comprising these two compounds in an amorphous state. The aim of obtaining an amorphous state was to overcome [...] Read more.
The co-administration of curcumin and hesperetin might be beneficial in terms of neuroprotective activity; therefore, in this study, we attempted to develop a fixed-dose formulation comprising these two compounds in an amorphous state. The aim of obtaining an amorphous state was to overcome the limitations of the low solubility of the active compounds. First, we assessed the possibility of using popular sweeteners (erythritol, xylitol, and sorbitol) as plasticizers to reduce the glass transition temperature of PVP K30 to prepare the polymer–excipient blends, which allowed the preparation of amorphous solid dispersions via hot-melt extrusion at a temperature below the original glass transition of PVP K30. Erythritol proved to be the superior plasticizer. Then, we focused on the development of fixed-dose amorphous solid dispersions of curcumin and hesperetin. Powder X-ray diffraction and thermal analysis confirmed the amorphous character of dispersions, whereas infrared spectroscopy helped to assess the presence of intermolecular interactions. The amorphous state of the produced dispersions was maintained for 6 months, as shown in a stability study. Pharmaceutical parameters such as dissolution rate, solubility, and in vitro permeability through artificial membranes were evaluated. The best improvement in these features was noted for the dispersion, which contained 15% of the total content of the active compounds with erythritol used as the plasticizer. Full article
(This article belongs to the Special Issue Further Research in Polyphenols Formulations)
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18 pages, 49677 KiB  
Article
Riparian Forests of Alnus Species Communities and Their Role in Sustainability in the Romanian Carpathians and Adjacent Regions
by Daniela Mogîldea, Tiberiu C. Sahlean and Claudia Biță-Nicolae
Sustainability 2024, 16(10), 4130; https://doi.org/10.3390/su16104130 (registering DOI) - 15 May 2024
Abstract
Alnus riparian communities are important for ecological stability. Data on Alnus species were gathered from herbaria and literature, revealing that 54.39% of the information refers to Alnus glutinosa, 39.42% to Alnus incana, and 6.18% to Alnus pubescens. This information highlights [...] Read more.
Alnus riparian communities are important for ecological stability. Data on Alnus species were gathered from herbaria and literature, revealing that 54.39% of the information refers to Alnus glutinosa, 39.42% to Alnus incana, and 6.18% to Alnus pubescens. This information highlights the widespread occurrence of A. glutinosa and A. incana, as described in distribution maps, contrasting with the more limited range of the hybrid A. pubescens. Principal component analysis (PCA), utilizing standardized factors, was carried out on 217 relevés of the communities of the studied species. In these relevés, we identified a total of 169 plant species, categorized as herbaceous (83%), trees (11%), and shrubs (6%). Three distinct forest communities, Stellario nemorum-Alnetum glutinosae, Alnetum incanae, and Telekio speciosae-Alnetum incanae, emerged from relevé groups. The distribution maps of the three taxa were overlaid on maps of the region’s protected areas, revealing a substantial presence of these taxa within the protected zones. The research aims to highlight the sustainability and conservation importance of Alnus communities in the Romanian Carpathians, to contribute to ongoing conservation efforts and promote the viability and resilience of these ecologically important wetland habitats. Full article
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20 pages, 3418 KiB  
Article
Renewable Energy Investments in Poland: Goals, Socio-Economic Benefits, and Development Directions
by Anna Dębicka, Karolina Olejniczak, Bartosz Radomski, Dariusz Kurz and Dawid Poddubiecki
Energies 2024, 17(10), 2374; https://doi.org/10.3390/en17102374 (registering DOI) - 15 May 2024
Abstract
Renewable energy sources (RES) will play a key role in the transition to clean energy. Financial and socio-economic benefits determine the investment management in these energy sources. This article aims to indicate current energy policy goals, present socio-economic benefits resulting from renewable energy [...] Read more.
Renewable energy sources (RES) will play a key role in the transition to clean energy. Financial and socio-economic benefits determine the investment management in these energy sources. This article aims to indicate current energy policy goals, present socio-economic benefits resulting from renewable energy investments, and review further development directions in Poland. The research was carried out using desk research, case studies, and literature review methods to provide a broader economic context for RES investments. The scope of the research included both the Polish and the European Union contexts. The authors examined the Polish objectives of investment in renewable energy contained in strategic, planning, and other legal documents compared to EU targets, reviewed possible investments in renewable energy, and indicated wind farms and photovoltaic investments as the most effective ones from the point of view of further development which aims to meet the EU’s goals by 2030. The authors also demonstrated a wide range of socio-economic benefits based on literature reviews, analysis of policy documents, and regulations regarding the energy sector, and examined a specific example of investment implementation and identified the ecosystem of beneficiaries and their benefits. Full article
(This article belongs to the Section A2: Solar Energy and Photovoltaic Systems)
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31 pages, 3930 KiB  
Article
Perception of Locals on Multiple Contributions of NTFPs to the Livelihoods of Forest Fringe Communities in Ghana
by Obed Asamoah, Jones Abrefa Danquah, Dastan Bamwesigye, Emmanuel Amoah Boakye, Mark Appiah and Ari Pappinen
Forests 2024, 15(5), 861; https://doi.org/10.3390/f15050861 (registering DOI) - 15 May 2024
Abstract
Forest-dwelling residents frequently collect non-timber forest products (NTFPs) for various reasons, such as food, medicine, firewood, religious reasons, or handicrafts. This study examines the multiple benefits derived from NTFPs and how they contribute to the livelihoods of Ghanaian communities to alleviate poverty. Resources [...] Read more.
Forest-dwelling residents frequently collect non-timber forest products (NTFPs) for various reasons, such as food, medicine, firewood, religious reasons, or handicrafts. This study examines the multiple benefits derived from NTFPs and how they contribute to the livelihoods of Ghanaian communities to alleviate poverty. Resources and services provided by NTFPs have an essential role in providing economic, nutritional, medicinal, cultural, and environmental benefits. This study examines the diverse range of NTFPs locals collect, such as chewing sticks, games, herbs, honey, leaves, mushrooms, pestles, raffia and palms, snails, and straws. In addition to serving different purposes, these NTFPs contribute to income generation, food security, health care, cultural practices, and the protection of the environment. A qualitative research approach was used in this study to collect data through semi-structured interviews and focus groups with members on multiple collections of NTFPs and their multiple benefits. We interviewed 732 residents regarding their multiple NTFPs and their potential to improve the livelihood of the locals. The data for the study were analysed using descriptive statistics. As a result of the survey, the frequency and percentages of responses were analysed for each indicator related to multiple values of NTFPs. Both males and females collected a list of NTFPs during the survey. Regarding NTFPs used for sale, 275 were reported, representing 37.57% of the total. In addition to emphasising the importance of sustainable management practices and equitable distribution of benefits, the study explores the multiple benefits of NTFPs for poverty alleviation in Ghanaian communities. It is essential to ensure the conservation of forest ecosystems and promote inclusive policies to harness the potential of NTFPs and maximise their positive impact on livelihoods. NTFPs provide multiple benefits for Ghanaian communities, including income, nutrition, healthcare, cultural preservation, and environmental sustainability. To promote sustainable development, it is imperative to understand the role of NTFPs and implement appropriate strategies. Full article
(This article belongs to the Special Issue Economic Valuation of Forest Resources)
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3 pages, 155 KiB  
Editorial
Special Issue: Molecular Ecology, Physiology and Biochemistry of Insects 3.0
by Klaus H. Hoffmann
Int. J. Mol. Sci. 2024, 25(10), 5390; https://doi.org/10.3390/ijms25105390 (registering DOI) - 15 May 2024
Abstract
With the outstanding work of Sir Vincent B [...] Full article
(This article belongs to the Special Issue Molecular Ecology, Physiology and Biochemistry of Insects 3.0)

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