Hepatitis C Virus (HCV) infection represents a significant global health challenge, with its natural course largely influenced by the host’s immune response. Human Leukocyte Antigen (HLA) molecules, particularly HLA class I and II, play a crucial role in the adaptive immune response against HCV. The polymorphism of HLA molecules contributes to the variability in immune response, affecting the outcomes of HCV infection. This study aims to investigate the frequency of HLA A, B, DR, and DQ alleles known to be associated with HCV clearance or persistence in a healthy Moroccan population. Conducted at the University Hospital Center Mohammed VI, Marrakech, this study spanned from 2015 to 2022 and included 703 healthy Moroccan individuals. HLA class I and II typing was performed using complement-dependent cytotoxicity and polymerase chain reaction-based methodologies. The results revealed the distinct patterns of HLA-A, B, DRB1, and DQB1 alleles in the Moroccan population. Notably, alleles linked to favorable HCV outcomes, such as HLA-DQB1*0301, DQB1*0501, and DRB1*1101, were more prevalent. Conversely, alleles associated with increased HCV susceptibility and persistence, such as HLA-DQB1*02 and DRB1*03, were also prominent. Gender-specific variations in allele frequencies were observed, providing insights into genetic influences on HCV infection outcomes. The findings align with global trends in HLA allele associations with HCV infection outcomes. The study emphasizes the role of host genetics in HCV infection, highlighting the need for further research in the Moroccan community, including HCV-infected individuals. The prevalence of certain HLA alleles, both protective and susceptibility-linked, underscores the potential for a national HLA data bank in Morocco. Full article

Previous research in Western nations has established a clear connection between pro-environmental public opinion and clean energy transition policies aligned with Sustainable Development Goals 7 and 13. However, little is known about this relationship in East Asia, the most important region of the world in terms of carbon emissions. Using the International Social Survey Program and Taiwan Social Change Survey results from 2010 and 2020, this study examines public opinion in Taiwan on environmental issues, comparing it with opinion in a group of 18 OECD countries. Results show high but stable support for the environment and the energy transition in Taiwan over this period, with no indications of climate denial. However, willingness to make sacrifices for the environment is sharply lower among the lower half of the income distribution, highlighting existing socioeconomic disparities and inequality. Further, political engagement around environmental issues remains relatively low in Taiwan compared to engagement in the OECD comparison group. This disjunction suggests a unique model of public opinion and policy outcomes in Taiwan, which is clearly distinct from patterns in the West. Comprehending this model is vital, considering East Asia’s necessary role in a global clean energy transition. Full article

Peripheral artery disease (PAD) is a common circulatory disorder characterized by the accumulation of fats, cholesterol, and other substances in the arteries that restrict blood flow to the extremities, especially the legs. The ankle brachial index (ABI) is a highly reliable and valid non-invasive test for diagnosing PAD. However, the traditional method has limitations. These include the time required, the need for Doppler equipment, the training of clinical staff, and patient discomfort. PWV refers to the speed at which an arterial pressure wave propagates along the arteries, and this speed is conditioned by arterial elasticity and stiffness. To address these limitations, we have developed a system that uses electrocardiogram (ECG) and photoplethysmography (PPG) signals to calculate pulse wave velocity (PWV). We propose determining the ABI based on this calculation. Validation was performed on 22 diabetic patients, and the results demonstrate the accuracy of the system, maintaining a margin of ±0.1 compared with the traditional method. This confirms the correlation between PWV and ABI and positions this technique as a promising alternative to overcome some of the limitations of the conventional method. Full article

Starting in the middle of March 2020, various lockdown measures and degrees of confinement were put in place in most European countries as a result of the COVID-19 pandemic. Although this situation meant that more people were likely to experience poorer mental health, largely due to the imposition of social distancing measures, many individuals incorporated music into their coping routines to help improve their psychological well-being. Using a gender perspective, this study analyzes how individuals used music during lockdown and explores the differences between men’s and women’s views on the impact that listening to and making music has on their perceived level of well-being. A questionnaire, MUSIVID19, was administered to a sample of 1868 participants from all the autonomous regions in Spain. The results reveal that in the uses and perceptions of music, the stereotypes underlying the patriarchal system were also reproduced during confinement. Full article

College students experiencing psychological distress have significantly greater negative emotions than students who practice compassionate thinking. We have developed Eight Steps to Great Compassion (ESGC), an innovative brief and no-cost online video training program about how to increase compassion among busy and young adult university students. To examine the effectiveness and benefits of the ESGC, a single-group pre-test–post-test quantitative design with undergraduate university students (N = 92; M_age = 20.39) evaluated its effects. The results from the post-test showed that the ESGC had a significant positive impact on increased feelings of compassion towards oneself, compassion for others, and the sense of personal well-being from the pre-test. The analysis of the PERMA-Profiler subscales also reflected a statistically significant increase in overall well-being and health and a decrease in negative emotions and loneliness. From the Post-Survey Lesson Feedback, 88% of the participants reported significant positive changes in themselves and the way that they live due to the program. These findings appear to show important implications for improving healthy minds and reducing negative emotions among university students. Full article

Leishmaniasis, caused by Leishmania parasites, is a neglected tropical disease and Cutaneous Leishmaniasis (CL) is the most common form. Despite the associated toxicity and adverse effects, Meglumine antimoniate (MA) remains the first-choice treatment for CL in Brazil, pressing the need for the development of better alternatives. Bacterial NanoCellulose (BNC), a biocompatible nanomaterial, has unique properties regarding wound healing. In a previous study, we showed that use of topical BNC + systemic MA significantly increased the cure rate of CL patients, compared to treatment with MA alone. Herein, we performed a study comparing the combination of a wound dressing (BNC or placebo) plus systemic MA versus systemic MA alone, in CL caused by Leishmania braziliensis. We show that patients treated with the combination treatment (BNC or placebo) + MA showed improved cure rates and decreased need for rescue treatment, although differences compared to controls (systemic MA alone) were not significant. However, the overall time-to-cure was significantly lower in groups treated with the combination treatment (BNC+ systemic MA or placebo + systemic MA) in comparison to controls (MA alone), indicating that the use of a wound dressing improves CL treatment outcome. Assessment of the immune response in peripheral blood showed an overall downmodulation in the inflammatory landscape and a significant decrease in the production of IL-1a (p < 0.05) in patients treated with topical BNC + systemic MA. Our results show that the application of wound dressings to CL lesions can improve chemotherapy outcome in CL caused by L. braziliensis. Full article

Due to aging and depopulation, cities in Japan struggle to maintain their municipal waste collection services. These challenges were exacerbated by the pandemic. To overcome these challenges, a prototype of collective and contactless waste collection technology has been developed. However, its acceptance by society is unknown. In this study, we surveyed Japanese people’s preferences regarding household waste disposal. The results showed that older adults (older than 60) are willing to walk longer (more than 2 min) to carry their waste to the disposal site than younger adults. They are also less concerned about the risk of disease infection from touching other people’s garbage than younger respondents (at a 0.24 count ratio). Other significant findings are that people who live alone prefer the temporary disposal site to be placed more than one minute away from their house (at a 0.19 count ratio). People living alone also produce less plastic and packaging waste than larger households. With more Japanese older adults living alone because of the scarcity of older-adult care facilities, we proposed two waste collection strategies that can allow for the implementation of more collective and automatized contactless waste pickup technology. Each design poses different challenges, such as the need for residents’ cooperation and a higher energy supply. However, they also open new opportunities, such as encouraging active aging and using renewable energy. Full article

Red clover (Trifolium pretense) is widely cultivated as an excellent forage and green manure crop. In 2021, a leaf spot disease was discovered in a red clover field in Min County, Gansu Province, China. Symptoms on T. pratense manifested as small white spots that gradually expanded into nearly oval or irregularly shaped gray-white lesions. The causal agent of this new disease was identified as Leptosphaeria weimeri (=Longiseptatispora meliloti) based on morphological identification, pathogenicity tests, and the phylogenetic identification of ITS, LSU, and SSU sequence. The optimal growth temperature was found to be 20 °C under different culture conditions, while the optimal spore-producing temperature was 25 °C. The pH for optimal growth and spore production was seven. The fungus grew and produced spores successfully on both PDA and PSA media. Additionally, the pathogen was efficiently inhibited using 450 g/L of prochloraz fungicide in vitro. To our knowledge, this is the first report of leaf spot disease on red clover caused by L. meliloti in China. Full article

This study presents an innovative lateral flow microfluidic paper-based analytical device (μPAD) designed for conducting quantitative paper-based enzyme-linked immunosorbent assays (p-ELISA), seamlessly executing conventional ELISA steps in a paper-based format. The p-ELISA device utilizes a passive fluidic circuit with functional elements such as a multi-bi-material cantilever (B-MaC) assembly, delay channels, and a buffer zone, all enclosed within housing for autonomous, sequential loading of critical reagents onto the detection zone. This novel approach not only demonstrates a rapid assay completion time of under 30 min, but also boasts reduced reagent requirements, minimal equipment needs, and broad applicability across clinical diagnostics and environmental surveillance. Through detailed descriptions of the design, materials, and fabrication methods for the multi-directional flow assay (MDFA), this manuscript highlights the device’s potential for complex biochemical analyses in a user-friendly and versatile format. Analytical performance evaluation, including a limit of detection (LOD) of 8.4 pM for Rabbit IgG, benchmarks the device’s efficacy compared to existing p-ELISA methodologies. This pioneering work lays the groundwork for future advancements in autonomous diagnostics, aiming to enhance global health outcomes through accessible and reliable testing solutions. Full article

To optimize the application of plant growth-promoting rhizobacteria (PGPR) in field trials, tracking methods are needed to assess their shelf life and to determine the elements affecting their effectiveness and their interactions with plants and native soil microbiota. This work developed a real-time PCR (qtPCR) method which traces and quantifies bacteria when added as microbial consortia, including five PGPR species: Burkholderia ambifaria, Bacillus amyloliquefaciens, Azotobacter chroococcum, Pseudomonas fluorescens, and Rahnella aquatilis. Through a literature search and in silico sequence analyses, a set of primer pairs which selectively tag three bacterial species (B. ambifaria, B. amyloliquefaciens and R. aquatilis) was retrieved. The primers were used to trace these microbial species in a field trial in which the consortium was tested as a biostimulant on two wheat varieties, in combination with biochar and the mycorrhizal fungus Rhizophagus intraradices. The qtPCR assay demonstrated that the targeted bacteria had colonized and grown into the soil, reaching a maximum of growth between 15 and 20 days after inoculum. The results also showed biochar had a positive effect on PGPR growth. In conclusion, qtPCR was once more an effective method to trace the fate of supplied bacterial species in the consortium when used as a cargo system for their delivery. Full article

Quantitative evaluation of the effects of diverse greenhouse vegetable production systems (GVPS) on vegetable yield, soil water consumption, and nitrogen (N) fates could provide a scientific basis for identifying optimum water and fertilizer management practices for GVPS. This research was conducted from 2013 to 2015 in a greenhouse vegetable field in Quzhou County, North China. Three production systems were designed: conventional (CON), integrated (INT), and organic (ORG) systems. The WHCNS-Veg model was employed for simulating vegetable growth, water dynamics, and fates of N, as well as water and N use efficiencies (WUE and NUE) for four continuous growing seasons. The simulation results revealed that nitrate leaching and gaseous N emissions constituted the predominant N loss within GVPS, which separately accounted for 11.5–59.4% and 6.0–21.1% of the N outputs. The order of vegetable yield, N uptake, WUE, and NUE under different production systems was ORG > INT > CON, while the order of nitrate leaching and gaseous N loss was CON > INT > ORG. Compared to CON, ORG exhibited a significant increase in yield, N uptake, WUE, and NUE by 24.6%, 24.2%, 26.1%, and 89.7%, respectively, alongside notable reductions in nitrate leaching and gaseous N loss by 67.7% and 63.2%, respectively. The ORG system should be recommended to local farmers. Full article

In this article, a comparison of ionization techniques is provided and discussed. Conventional liquid chromatography with an electrospray ionization source shows higher robustness and repeatability in comparison with liquid chromatography coupled with a coordination ion spray (CIS-MS) source using silver nitrate as the dopant. However, the higher sensitivity and possibility to collect more data in untargeted applications mean CIS-MS is emerging as an instrument used in specific applications. During this research, the limit of detection (LOD) for GHRP-2 and GHRP-6 was established at 0.2 ng/mL, and the lower limit of quantification (LLOQ) was 0.5 ng/mL for CIS-MS. For conventional ESI-MS combined with solid-phase extraction on weak cation exchange columns, the limit of detection was found to be 1 ng/mL, and the lower limit of quantification was 2 ng/mL. Full article

Locally advanced breast cancer (LABC) is a complex disease that requires a multidisciplinary approach. Neoadjuvant chemotherapy (NAC) is usually performed in order to achieve loco-regional radical resection; although its importance in the multidisciplinary approach to LABC is well recognized, a small number of patients show Progressive Disease (PD). No standard salvage treatment (ST) has been defined and different strategies can be adopted, such as second-line systemic therapies, radiation therapy, and surgery. Herein, a case of LABC in PD during NAC is reported with a literature review, with the aim of highlighting the importance of a tailored multidisciplinary treatment for each patient. Full article

Natural resources must be administered efficiently to reduce the human footprint and ensure the sustainability of the planet. Water is one of the most essential resources in agriculture. Modern information technologies are being introduced in agriculture to improve the performance of agricultural processes while optimizing water usage. In this scenario, artificial intelligence techniques may become a very powerful tool to improve efficiency. The introduction of the edge/fog/cloud paradigms, already adopted in other domains, may help to organize the services involved in complex agricultural applications. This article proposes the combination of several modern technologies to improve the management of hydrological resources and reduce water waste. The selected technologies are (1) fuzzy logic, used for control tasks since it adapts very well to the nonlinear nature of the agricultural processes, and (2) long range (LoRa) technology, suitable for establishing large distance links among the field devices (sensors and actuators) and the process controllers, executed in a centralized way. The presented approach has been validated in the laboratory by means of a control scheme aimed at achieving an adequate moisture level in the soil. The control algorithm, based on fuzzy logic, can use the weather forecast, obtained as a cloud service, to reduce water consumption. For testing purposes, the dynamics of the water balance model of the soil were implemented as hardware in the loop, executed in a dSPACE DS1104. Experiments proved the viability of the presented approach since the continuous space state output controller achieved a water loss reduction of 23.1% over a 4-day experiment length compared to a traditional on/off controller. The introduction of cloud services for weather forecasting improved the water reduction by achieving an additional reduction of 4.07% in water usage. Full article

Background/Objectives: Extrauterine growth restriction (EUGR) is associated with high mortality and an increased incidence of poor neurodevelopmental outcomes in preterm infants. In this study, we aimed to compare the Intergrowth-21^ST (IG-21^ST) and Fenton charts in predicting long-term neurodevelopmental and anthropometric outcomes of very low birth weight (VLBW) infants. Methods: Data were collected from 2649 VLBW infants registered in the Korean Neonatal Network born between 24^0/7 and 31^6/7 weeks of gestational age from January 2013 to December 2017. Follow-up assessments were conducted at 18–24 months of age, corrected for prematurity. Multiple logistic regression analysis was performed to evaluate the association between EUGR and long-term outcomes. Results: Among the 2649 VLBW infants, 60.0% (1606/2649) and 36.9% (977/2649) were diagnosed as having EUGR defined by the Fenton chart (EUGR_F) and by the IG-21^ST chart (EUGR_IG), respectively. The EUGR_IG group exhibited a higher proportion of infants with cerebral palsy, neurodevelopmental impairment (NDI), and growth failure. In multiple logistic regression analysis, adjusted for risk factors for long-term outcome, the EUGR_IG group showed higher risk of cerebral palsy (adjusted odds ratio [aOR], 1.66; 95% confidence interval [CI], 1.04–2.65), NDI (aOR, 2.09; 95% CI, 1.71–2.55), and growth failure (aOR, 1.57; 95% CI, 1.16–2.13). Infants with EUGR_F tended to develop NDI (aOR, 1.29; 95%CI, 1.03–1.63) and experience growth failure (aOR, 2.44; 95% CI, 1.77–3.40). Conclusions: The IG-21^ST chart demonstrated a more effective prediction of long-term neurodevelopmental outcomes, whereas the Fenton chart may be more suitable for predicting growth failure at 18–24 months. Full article

All water destined for human consumption must be subjected to disinfection processes via chlorination with the aim of eliminating the risk of the transmission of waterborne diseases. However, the treatment of water using this method leads to the formation of trihalomethanes (THMs), which are toxic compounds that may be ingested, inhaled, or absorbed by the skin, increasing the risk of carcinogenic and mutagenic processes. High trihalomethane concentrations in water may be directly related to physicochemical properties, such as temperature, pH, hardness, and potentially toxic metal concentrations. In this work, physicochemical characterization was performed and water quality was assessed with regard to the presence of trihalomethanes in 17 samples collected from points before and after storage in reservoirs. Atomic absorption spectroscopy to determine potentially toxic metals and the measurement of physicochemical properties demonstrated that all results were in accordance with the standards established by Brazilian legislation. Gas chromatography–mass spectrometry was used to determine levels of trihalomethanes in the water samples (trichloromethane, bromodichloromethane, chlorodibromomethane, and bromoform), which were also in accordance with the limits established by Brazilian legislation. Hierarchical clustering on principal components confirmed changes in the water quality depending on location. This work constitutes a paradigm for future studies on the monitoring of toxic organic compounds in water to avoid health problems in humans and animals. Full article

Psychiatric disorders often require pharmacological interventions to alleviate symptoms and improve quality of life. However, achieving an optimal therapeutic outcome is challenging due to several factors, including variability in the individual response, inter-individual differences in drug metabolism, and drug interactions in polytherapy. Therapeutic drug monitoring (TDM), by measuring drug concentrations in biological samples, represents a valuable tool to address these challenges, by tailoring medication regimens to each individual. This review analyzes the current landscape of TDM in psychiatric practice, highlighting its significance in optimizing drug dosages, minimizing adverse effects, and improving therapeutic efficacy. The metabolism of psychiatric medications (i.e., mood stabilizers, antipsychotics, antidepressants) often exhibits significant inter-patient variability. TDM can help address this variability by enhancing treatment personalization, facilitating early suboptimal- or toxic-level detection, and allowing for timely interventions to prevent treatment failure or adverse effects. Furthermore, this review briefly discusses technological advancements and analytical methods supporting the implementation of TDM in psychiatric settings. These innovations enable quick and cost-effective drug concentration measurements, fostering the widespread adoption of TDM as a routine practice in psychiatric care. In conclusion, the integration of TDM in psychiatry can improve treatment outcomes by individualizing medication regimens within the so-called precision medicine. Full article

Biochar (Bc) and liquid vermicompost extracts (LVEs) are increasingly being used as biofertilizers in agriculture to promote soil-microbe-crop interactions. However, although both these products can potentially act synergistically due to their complementary characteristics, their co-application in different soils has not yet been investigated. Therefore, firstly, an LVE-activated biochar (B_LVE) was experimentally formulated and the persistence of LVE bacteria over a 60-day storage period was determined. The total number of LVE bacteria increased by 10-fold after 7 days and was stable throughout the entire biochar storage period. In addition, changes in the composition of the bacterial community were observed after 30 days of storage, indicating that taxa less represented in pure LVE may be advantaged upon biochar colonization. Secondly, a microcosm experiment was performed to evaluate whether the biological fertility and enzyme activities of two soils, differing in organic matter content, could be enhanced by the addition of LVE-activated biochar. In this experiment, three different doses of Bc, LVE, and B_LVE against the carbon-related biological fertility index (i.e., biological fertility index, BFI) and three enzyme activities over a 21-day incubation period were tested. The B_LVE treatment yielded the best results (i.e., BFI +32%, enzyme activities +38%). This indicates that Bc and LVEs can act synergistically to promote soil fertility, quality, and microbial activity. By integrating LVE-activated biochar into their soil management practices, farmers could achieve higher crop yields and healthier products. Full article

In human–computer interaction interfaces, icons serve as highly symbolic elements that convey information and significantly influence the performance of visual search and other tasks. Thus, the selection of appropriate colors plays a crucial role in the design of human–computer interfaces. This study aimed to investigate the effects of icon color combinations on human visual search task performance across various cognitive load conditions. The experiment was divided into two parts, involving a cognitive load test experiment and an icon search task, wherein the former required participants to select the target icon from 16 de-colored icons, and the latter involved selecting the target icon from 16 color combinations of 8 colors (background colors: black, red, blue, and purple; foreground colors: white, yellow, green, and turquoise). A total of 20 participants (11 females and 9 males, all aged between 18 and 24 years) were recruited for this experiment to perform both tasks sequentially. Through a comprehensive consideration of factors such as the NASA-TLX scale, retrieval time, etc., the experimental results revealed that cognitive load increased with time pressure, resulting in varying optimal color combinations for different cognitive load levels. Additionally, background colors did not affect task response time under different cognitive loads, whereas white foreground color was superior to turquoise foreground color in a medium cognitive load environment. Meanwhile, our results recommend prioritizing white-on-black as the preferred color combination, given that it demonstrated excellent cognitive performance in all three cognitive load environments. Conversely, white-on-blue is not recommended as a color combination for the design of high cognitive load environments. Concerning medium cognitive load environments, yellow-on-red or white-on-purple color combinations were preferred. Finally, in low cognitive load environments, white-on-purple is recommended as the primary color combination. Overall, this study provides a theoretical reference for the future design of interactive interface icons across various contexts. Full article

In order to improve the plugging performance of high-temperature and high-salt oil reservoir plugging agents, this paper utilizes a copolymer composed of acrylamide and 2-acrylamide-2-methylpropanesulfonic acid (AM/AMPS) as the polymer, polyethyleneimine as the cross-linking agent, and nylon fiber as the stabilizer to develop a high-temperature- and high-salt-resistant gel system. This study analyzed and evaluated the temperature resistance, salt resistance and blocking performance of the gel system. The evaluation results show that the gel-forming strength of this gel system can reach an H level, and it has good thermal stability at the high temperature of 130 °C. At the high salinity of 240,720 mg/L, the syneresis rate remains below 2.5%, and the gel-forming time is greater than 15 h; the higher the temperature, the shorter the gelling time. The results of our sand-filled pipe-plugging experiment show that the gel system can adapt to sand-filled pipes with different levels of permeability, and reaching a plugging rate of 94%. Full article

Exact biopsy planning and careful execution of needle injection is crucial to ensure successful procedure completion as initially intended while minimizing the risk of complications. This study introduces a solution aimed at helping the operator navigate to precisely position the needle in a previously planned trajectory utilizing a mixed reality headset. A markerless needle tracking method was developed by integrating deep learning and deterministic computer vision techniques. The system is based on superimposing imaging data onto the patient’s body in order to directly perceive the anatomy and determine a path from the selected injection site to the target location. Four types of tests were conducted to assess the system’s performance: measuring the accuracy of needle pose estimation, determining the distance between injection sites and designated targets, evaluating the efficiency of material collection, and comparing procedure time and number of punctures required with and without the system. These tests, involving both phantoms and physician participation in the latter two, demonstrated the accuracy and usability of the proposed solution. The results showcased a significant improvement, with a reduction in number of punctures needed to reach the target location. The test was successfully completed on the first attempt in 70% of cases, as opposed to only 20% without the system. Additionally, there was a 53% reduction in procedure time, validating the effectiveness of the system. Full article

As the population ages, the incidence of age-related neurodegenerative diseases is rapidly increasing, and novel approaches to mitigate this soaring prevalence are sorely needed. Recent studies have highlighted the importance of gut microbial homeostasis and its impact on brain functions, commonly referred to as the gut–brain axis, in maintaining overall health and wellbeing. Nonetheless, the mechanisms by which this system acts remains poorly defined. In this review, we will explore how (poly)phenols, a class of natural compounds found in many plant-based foods and beverages, can modulate the gut–brain axis, and thereby promote neural health. While evidence indicates a beneficial role of (poly)phenol consumption as part of a balanced diet, human studies are scarce and mechanistic insight is still lacking. In this regard, we make the case that dietary (poly)phenols should be further explored to establish their therapeutic efficacy on brain health through modulation of the gut–brain axis, with much greater emphasis on carefully designed human interventions. Full article