This article presents the results of almost nine months of ethnographic research on the relationship between physical exercise and health in older people in the post-COVID-19 context. Via exploratory–descriptive qualitative research and the use of a convenient sample, I shed light on this relationship using the stories and life experiences of 40 older people (10 men and 30 women, including two women instructors for senior classes) who exercise regularly. The meanings they attributed to physical exercise during COVID-19 and after it are explained, emphasising first that there is no health in a context of not feeling safe; once there is a feeling of security, the most relevant meanings can be exposed in three directions. First, exercise produces a sense of identity linked to “being an athlete” and “belonging to a group”. Second, exercising is valued as participating in something meaningful (the meanings range from self-realisation, independence, and autonomy to feelings of happiness). Finally, and linked to the sense of identity, those who train alone show more commitment and total hours spent in physical exercise and physical activity than those who train in groups. Even though older people are not a homogeneous group, they generally faced the pandemic as an ageist situation that affected their lives and how they saw sports and health. This article describes the strategies they used during COVID-19 related to exercise and well-being and those used once the pandemic restrictions were no longer present. The qualitative aspects that physical exercise brings to this population are highlighted. The research results give voice to older people, showing their heterogeneity and the meanings and practices that unite them. These inputs are rich material for studies on physical activity, older people, and well-being. Full article

Recent experiments devoted to characterizing the behavior of sessile ferroelectric liquid droplets on ferroelectric solid substrates have shown the existence of a droplet electromechanical Rayleigh-like instability. The instability is induced by the bulk polarization of the ferroelectric fluid, which couples to the polarization of the underlying substrate through its fringing field and solid–fluid interface coupling. With the aim of characterizing this phenomenon, namely the coupling between the polarizations of a fluid and a solid material, we studied the behavior of ferroelectric liquid droplets confined between two solid substrates, arranged in different configurations, realized to generate fringing fields with different profiles. The results show that the features of the droplets instability are indeed affected by the specific fringing field shape in a way dominated by the minimization of the electrostatic energy associated with the bulk polarization of the ferroelectric fluid. Full article

PD-L1 immunohistochemistry (IHC) has become an established method for predicting cancer response to targeted anti-PD1 immunotherapies, including breast cancer (BC). The alternative PD-1 ligand, PD-L2, remains understudied but may be a complementary predictive marker. Prospective analysis of 32 breast cancers revealed divergent expression patterns of PD-L1 and PD-L2. PD-L1-positivity was higher in immune cells than in cancer cells (median = 5.0% vs. 0.0%; p = 0.001), whereas PD-L2-positivity was higher in cancer cells than immune cells (median = 30% vs. 5.0%; p = 0.001). Percent positivity of PD-L1 and PD-L2 were not correlated, either in cancer cells or immune cells. Based on a cut-point of >1% positivity, ER+ tumors (n = 23) were frequently PD-L2-positive (73.9%), whereas only 40.9% were PD-L1-positive. These data suggest differential control of cellular PD-L1 and PD-L2 expression in BC and a potential role for PD-L2 IHC as a complementary marker to PD-L1 to improve selection of aggressive ER+ BC that may benefit from anti-PD-1 therapy. Full article

By producing high quality food with a low environmental impact, organic farming plays an essential role in developing sustainable food systems. Over time, consumer interest in organic food is systematically growing, which results in a generally positive attitude towards organic products. Many studies that are devoted to analysing organic food consumers’ behaviour aim to generalise the studied trends to the population of a given country or region. Given the varying rates of organic market development between countries, there is a need for more narrowly focused research, which could more profoundly facilitate local development of organic market. The aim of this study was to investigate the opinions of Polish consumers about organic fruit and to identify the socioeconomic and sociodemographic determinants of the propensity to choose and purchase organic fruit. Respondents were recruited via a market research agency using a consumer access panel. To collect responses, a formal, structured questionnaire was developed and distributed using the CAWI method. The general acceptance of price increases for organic apples amounts to less than around 30% (89% of responses). The effect of income and package size tend to interact with regard to the purchase preference of apples (p < 0.001). The highest frequency of organic fruit purchases were indicated for physical stores, in particular supermarkets (5.35 times/month, 95%CI). A relationship between willingness to consume organic fruits and the self-described economic situation (p = 0.005), as well as true household income (p = 0.007), has been shown. A relationship between the frequency of organic fruit purchases and the number of household members (p = 0.006), as well as number of household members under the age of 18 (p = 0.011), has also been shown. Marketing techniques for organic products should emphasize family welfare and be directed at younger consumers, especially at retail outlets where customers are most likely to buy them, namely physical supermarkets, local grocery stores and discount stores. Full article

Black soldier fly larvae (BSFL) can utilize food by-products or residues for growth, benefiting farm animal’s diets’ production sustainability. The experiment aimed to assess the effect of different substrate compositions on larval growth, chemical composition, and substrate temperature. BSFL were allocated to one of the four diets (control, vegetable, carnivorous, and omnivorous) for the entire experiment (8 days). The temperature was measured twice daily using a thermal-imaging camera, and the accumulated degree hours (ADH) was calculated. The results showed that the larvae fed the vegetable diet exhibited a significantly reduced growth performance, with a biomass reduction of 26.3% compared to the control diet; furthermore, vegetable-fed larvae showed a lower dry matter content (−30% compared to the average of other diets) due to lower fat content (−65% compared to average of other diets). The nutritional composition of larvae fed an omnivorous diet was similar to larvae fed a high-quality substrate diet (control diet-chicken feed), indicating that the omnivorous diet could be an ideal solution for rearing BSFL larvae; however, the current European legislation prohibits the use of animal meal. The study also revealed that substrate temperatures did not have a discernible influence on larval growth, further emphasizing the importance of diet in BSFL rearing strategies. Full article

Methionine dipeptide (Met-Met) could improve milk protein synthesis in bovine epithelia mammary cells and lactating mice, while the effects of Met-Met on lactation performance, rumen fermentation and microbiota profile in lactating dairy cows have not been explored. For this reason, 60 Chinese lactating Holstein cows were allocated into three treatment groups: control group (CON), 6 g/d methionine dipeptide group (MM), and 6.12 g/d rumen-protected methionine dipeptide group (RPMM). The experiment lasted for 10 weeks to monitor lactation performance, plasma amino acid profile and rumen fermentation parameters and microbiota profile. Results showed that MM increased the energy-corrected milk (ECM), and RPMM increased both milk yield and ECM (p < 0.05). The milk protein concentration and yield were increased by MM and RPMM (p < 0.05). The rumen fermentation showed that RPMM increased total volatile fatty acids, acetate and valerate concentrations (p < 0.05). The relative abundance of Firmicutes, including Succiniclasticum, Selenomonas and Clostridium_XlVa, were enriched and the Prevotella was decreased by RPMM (p < 0.05). In summary, daily supplementing with 6 g of MM or RPMM in lactating dairy cows could improve milk yield and both percentage and yield of milk protein, and RPMM benefited the rumen fermentation and altered the bacterial composition. These results provided the first evidence that Met-Met supplementation can improve lactation performance of dairy cows. Full article

Microplastics (MPs) and antibiotics are emerging pollutants widely found in aquatic environments, potentially causing environmental harm. MPs may act as carriers for antibiotics, affecting their environmental distribution. This study investigates the adsorption of four macrolide antibiotics and a metabolite onto two types of MPs: polyethylene terephthalate (PET) and polyethylene (PE). Results revealed a linear isotherm adsorption model, with higher adsorption to PET than to PE (R² > 0.936 for PE and R² > 0.910 for PET). Hydrophobic interactions and hydrogen bonding could be the main adsorption mechanisms, with pore filling potentially involved. Reduced particle size enhances adsorption due to the increase of active adsorption sites. This increasement is more pronounced in PE than in PET, leading to an 11.6% increase in the average adsorption of all macrolides to PE, compared to only 5.1% to PET. Dissolved organic matter inhibits adsorption (azithromycin adsorption to PE was reduced from 12% to 5.1%), while salinity enhances it just until 1% salinity. pH slightly influences adsorption, with maximal adsorption at neutral pH. Results in real samples showed that complexity of the matrix decreased adsorption. Overall, these findings indicate that PE and PET MPs can be a vector of macrolides in aquatic environments. Full article

Derangement of the epidermal barrier lipids and dysregulated immune responses are key pathogenic features of atopic dermatitis (AD). The Th2-type cytokines interleukin IL-4 and IL-13 play a prominent role in AD by activating the Janus Kinase/Signal Transduction and Activator of Transcription (JAK/STAT) intracellular signaling axis. This study aimed to investigate the role of JAK/STAT in the lipid perturbations induced by Th2 signaling in 3D epidermal equivalents. Tofacitinib, a low-molecular-mass JAK inhibitor, was used to screen for JAK/STAT-mediated deregulation of lipid metabolism. Th2 cytokines decreased the expression of elongases 1, 3, and 4 and serine-palmitoyl-transferase and increased that of sphingolipid delta(4)-desaturase and carbonic anhydrase 2. Th2 cytokines inhibited the synthesis of palmitoleic acid and caused depletion of triglycerides, in association with altered phosphatidylcholine profiles and fatty acid (FA) metabolism. Overall, the ceramide profiles were minimally affected. Except for most sphingolipids and very-long-chain FAs, the effects of Th2 on lipid pathways were reversed by co-treatment with tofacitinib. An increase in the mRNA levels of CPT1A and ACAT1, reduced by tofacitinib, suggests that Th2 cytokines promote FA beta-oxidation. In conclusion, pharmacological inhibition of JAK/STAT activation prevents the lipid disruption caused by the halted homeostasis of FA metabolism. Full article

Methoxy poly(ethylene glycol)-block-poly(L-lactide) (MPEG-b-PLLA) has a wide range of applications in pharmaceuticals and biology, and its structure and morphology have been thoroughly studied. In the experiment, we synthesized MPEG-b-PLLA with different block lengths using the principle of ring-opening polymerization by controlling the amount of lactic acid added. The thermodynamic properties of copolymers and the crystallization properties of blends were studied separately. The crystallization kinetics of PDLA/MPEG-b-PLA and PLLA/MPEG-b-PLA composite films were studied using differential scanning calorimetry (DSC). The results indicate that the crystallization kinetics of composite films are closely related to the amount of block addition. The crystallinity of the sample first increases and then decreases with an increase in MPEG-b-PLLA content. These results were also confirmed in polarized optical microscope (POM) and wide-angle X-ray diffraction (WAXD) tests. When 3% MPEG-b-PLLA was added to the PDLA matrix, the blend exhibited the strongest crystallization performance. Full article

To explore the evolution in patterns of urban water consumption (UWC) accompanying socio-economic development, historical data on socio-economic conditions and water consumption in developed cities worldwide were collected. stages of evolution and patterns in UWC were identified through Pettitt tests. Through correlation analysis, the main socio-economic indicators influencing UWC were identified, and their threshold values for different trends in the evolution of UWC were statistically analyzed. The use of these patterns of evolution for UWC prediction was explored taking Changsha, China as a case study. Results indicate the following: (1) UWC typically increases in the early stage of development and then stabilizes or decreases later; (2) when UWC stabilizes or decreases, socio-economic indicators, namely per capita GDP, tertiary industry’s contribution to GDP, and urbanization rate, range from [USD 10,000, 60,000], [60%, 80%], and [85%, 95%], respectively, entering or approaching the developed economy stage. (3) Using patterns in this evolution, Changsha’s UWC is predicted to increase until the turning point, which will occur between 2027 and 2035 (earlier than that predicted using genetic programming) and then decrease earlier than that of Hunan Province or the entirety of China. Predicted values are lower than those obtained using the quota method. This study provides a new perspective on relationships between UWC and socio-economic conditions, supporting water resources planning. Full article

The reliability of friction stir welded joints is a critical concern, particularly given their potential applications in the aerospace manufacturing industry. This study offers a quasi-in situ observation of the microstructural response during fatigue crack growth (FCG) of a friction stir welded AA2024-T4 joint, aiming to correlate fatigue crack growth behavior with mechanical properties investigated using electron backscatter diffraction (EBSD). Notched compact tension (CT) specimens corresponding to the morphology of the stir zone (SZ), advancing side (AS), and retreating side (RS) were meticulously designed. The findings indicate that the welding process enhances the joint’s resistance to fatigue crack growth, with the base metal exhibiting a shorter fatigue life (i.e., ~10⁵ cycles) compared to the welding zones (SZ ~ 3.5 × 10⁵ cycles, AS ~ 2.5 × 10⁵ cycles, and RS ~ 3.0 × 10⁵ cycles). Crack propagation occurs within the stir zone, traversing refined grains, which primarily contribute to the highest fatigue life and lowest FCG rate. Additionally, cracks initiate in AS and RS, subsequently expanding into the base metal. Moreover, the study reveals a significant release of residual strain at the joint, particularly notable in the Structural-CT-RS (Str-CT-RS) sample compared to the Str-CT-AS sample during the FCG process. Consequently, the FCG rate of Str-CT-AS is higher than that of Str-CT-RS. These findings have significant implications for improving the reliability and performance of aerospace components. Full article

Myocardial infarction (MI), including ST-segment elevation MI (STEMI) and non-ST-segment elevation MI (NSTEMI), is still a leading cause of death worldwide. Metabolomics technology was used to explore differential metabolites (DMs) as potential biomarkers for early diagnosis of STEMI and NSTEMI. In the study, 2531 metabolites, including 1925 DMs, were discovered. In the selected 27 DMs, 14 were successfully verified in a new cohort, and the AUC values were all above 0.8. There were 10 in STEMI group, namely L-aspartic acid, L-acetylcarnitine, acetylglycine, decanoylcarnitine, hydroxyphenyllactic acid, ferulic acid, itaconic acid, lauroylcarnitine, myristoylcarnitine, and cis-4-hydroxy-D-proline, and 5 in NSTEMI group, namely L-aspartic acid, arachidonic acid, palmitoleic acid, D-aspartic acid, and palmitelaidic acid. These 14 DMs may be developed as biomarkers for the early diagnosis of MI with high sensitivity and specificity. These findings have particularly important clinical significance for NSTEMI patients because these patients have no typical ECG changes. Full article

The COVID-19 pandemic represents a quintessential public health crisis, profoundly impacting the utilization patterns of urban green spaces through stringent quarantine and lockdown measures. However, existing research inadequately addresses specific concerns regarding future urban green spaces and tends to oversimplify population divisions. This study delves into the needs and preferences of Shanghai residents affected by the pandemic and quarantine measures, focusing on various aspects such as specific types of green spaces, facilities, landscape elements, and landscape and spatial types. Multifactorial population clustering was also performed. This study delineates the following conclusions: (1) It is imperative to afford residents access to green spaces at least once a week, even during quarantine periods. (2) Residents exhibited a preference for accessible green spaces equipped with essential amenities, favoring unobstructed vistas and plant-centric ecological landscapes during the pandemic. Additionally, there is a notable preference for private green spaces among residents. (3) Post-pandemic, the “affluent” group displays a heightened overall demand for green spaces, the “middle-class” group shows a conspicuous inclination towards specific green space landscape elements, while the “low-income” group consistently exhibits a low preference for green spaces during and after the pandemic. This study underscores the necessity of developing human-centric green spaces to promote equity and resilience in the face of future emergencies, rooted in residents’ preferences amidst public health crises. Full article

Background: Non-melanoma skin cancers (NMSCs) are responsible for up to one-third of all human malignancies. Surgery is usually the treatment of choice, but patients often experience pain during the procedure. Topical rhenium-188 resin skin cancer treatment (RSCT) may be a valid therapeutic alternative. Methods: In this monocentric pilot study, 19 patients suffering from NMSC were treated with RSCT. Most of these patients had also experienced surgery, either because they developed a new NMSC in aftercare, or they had suffered previously from NMSC. Three RSCT-treated patients, who had no exposure to surgery so far, were paired with three matched patients, who had received surgery. We sought to evaluate and compare the patients’ experience with both treatments. A questionnaire assessed patients’ perceptions regarding side effects, aesthetic outcomes, wound care, fear of complications, and personal treatment preferences. Patients evaluated the different parameters of their either RSCT- or surgery-treated lesions on a scale from 0–10. Results: Patients were more afraid of complications before surgery than before RSCT (p = 0.04). Treatment with RSCT caused significantly less pain on treatment day (mean 0.56) than surgery (mean 2.32) (0 no pain, 10 maximum pain) (p = 0.02) and 14 days after the procedure (mean 0.89 versus mean 2.47) (p = 0.02). On day 14, RSCT-treated lesions were also significantly less itchy (mean 0.34) than after surgery (mean 1.50). Most patients were very satisfied with the aesthetic outcome after both RSCT (mean 8.42) and surgery (mean 8.31) (p = 0.89). In the case of a new NMSC, the majority of patients who experienced both treatments would rather be treated primarily with RSCT (44%) or would consider both options (31%); only 19% preferred surgery. Conclusion: Patients evaluated RSCT as less painful than surgery. The aesthetic outcomes of both treatments were comparable. For pain-sensitive patients, RSCT might be a preferable treatment option. Full article

This research investigates how different fermentation techniques using non-Saccharomyces yeast (Candida ethanolica Ce, Hanseniaspora guilliermondii Hg, Hanseniaspora thailandica Ht) and Saccharomyces cerevisiae (Sc) affect the synthesis of hesperidin, nobiletin, and other flavonoid and aromatic substances, which play a vital role in improving the overall quality of fruit wines due to their various biological properties. The combination of Sc:(Ce.Ht)-1:100 (Ce 0.5 × 10⁷ CFU/mL, Ht 0.5 × 10⁷ CFU/mL, Sc 1 × 10⁵ CFU/mL) yielded the highest hesperidin content at 4.12 ± 0.08 mg/L, followed by the Sc:(Ce.Hg)-1:1 (Ce 0.5 × 10⁷ CFU/mL, Hg 0.5 × 10⁷ CFU/mL, Sc 1 × 10⁷ CFU/mL) combination at 4.08 ± 0.06 mg/L. The highest nobiletin content was achieved by the (Hg.Ht)-10-Sc (Hg 0.5 × 10⁷ CFU/mL, Ht 0.5 × 10⁷ CFU/mL, Sc 1 × 10⁷ CFU/mL) combination, reaching 1.04 ± 0.05 mg/L, which was significantly higher than other multi-strain combinations. Additionally, the hesperidin content produced by the (Hg.Ht)-10-Sc combination was relatively high at 4.04 ± 0.02 mg/L, demonstrating a richness and complexity of aroma superior to that of fermentation with commercial yeast strains alone. The findings suggest that the (Hg.Ht)-10-Sc combination is the most effective multi-strain combination for increasing the levels of nobiletin and hesperidin in citrus wine, thereby enhancing the overall quality of the wine. These experimental results offer a promising approach for enhancing the quality of citrus wines and other fruit wines. Full article

Multiple-thermal-fluid (MTF) stimulation technology has been successfully applied in heavy-oil reservoir development, resulting in the significant enhancement of oil production. However, the underlying mechanism of multi-component coupling remains unclear. This paper constructs a coupling model for MTF stimulation, investigates the coupling mechanism of different media in various zones during multiple-cycle stimulation operations, and compares the implementation effect with field results. The findings reveal that (1) based on media distribution, the area from near-wellbore to far well locations can be divided into four zones: high-temperature oil-viscosity-reduction zones, compound action zones, energy-replenishment zones, and unaffected zones. (2) In the high-temperature oil-viscosity-reduction zone, the latent heat of vaporization is released by steam, and ultra-heavy oil absorbs heat and reduces its viscosity, which plays a dominant role in the production of MTF. In the compound action zone, hot water, CO₂, and N₂ exhibit a synergistic effect which enhances overall performance. In the energy-replenishment zone, a small amount of N₂ provides pressure maintenance and an additional energy supply. (3) As more cycles of stimulation are conducted, the compound action zone expands, while the energy-replenishment zone contracts. Simultaneously, there is a decrease in contribution rate from the high-temperature viscosity-reduction zone to oil production but an increase from both the compound action zone and energy-replenishment zone up to 30%. Based on the dynamic law of representative wells, this paper proposes a multi-media zonal coupling mechanism, providing a reference for subsequent research on MTF stimulation mechanisms and the adjustment of production measures. Full article

The material developed focuses on the analysis of energy efficiency trends in Poland, utilising ODEX indicators for the sectors of industry, transport, households and in general, from 2011 to 2021. The objective of the study is to assess the progress made in energy efficiency and to forecast future trends in these sectors. The methods employed are based on statistical modelling of time series, taking into account sector-specific energy consumption dynamics. The following techniques were employed: linear regression, cluster analysis to identify patterns of change, statistical hypothesis testing for energy efficiency and simplified autoregressive models. The results demonstrated significant improvements in energy efficiency in the industrial sector, stability of the ODEX indicator in the transport sector and gradual improvements in households and overall. The prediction indicates an upward trend in the ODEX indicator in the short term, suggesting an increase in energy demand. However, it also predicts a decline in the long term, which may indicate the effectiveness of future energy efficiency strategies and investments. Consequently, the necessity for continued efforts to increase energy efficiency and further research into the factors influencing energy efficiency in different economic sectors is emphasised. Full article

This work presents the results of an advanced geophysical characterization of a contaminated site, where a correct understanding of the dynamics in the unsaturated zone is fundamental to evaluate the effective management of the remediation strategies. Large-scale surface electrical resistivity tomography (ERT) was used to perform a preliminary assessment of the structure in a thick unsaturated zone and to detect the presence of a thin layer of clay supporting an overlying thin perched aquifer. Discontinuities in this clay layer have an enormous impact on the infiltration processes of both water and solutes, including contaminants. In the case here presented, the technical strategy is to interrupt the continuity of the clay layer upstream of the investigated site in order to prevent most of the subsurface water flow from reaching the contaminated area. Therefore, a deep trench was dug upstream of the site and, in order to evaluate the effectiveness of this approach in facilitating water infiltration into the underlying aquifer, a forced infiltration experiment was carried out and monitored using ERT and ground-penetrating radar (GPR) measurements in a cross-hole time-lapse configuration. The results of the forced infiltration experiment are presented here, with a particular emphasis on the contribution of hydro-geophysical methods to the general understanding of the subsurface water dynamics at this complex site. Full article

Central Nervous System Germ Cell Tumors (CNS GCTs) represent a subtype of intracranial malignant tumors characterized by highly heterogeneous histology. Current diagnostic methods in clinical practice have notable limitations, and treatment strategies struggle to achieve personalized therapy based on patient risk stratification. Advances in molecular genetics, biology, epigenetics, and understanding of the tumor microenvironment suggest the diagnostic potential of associated molecular alterations, aiding risk subgroup identification at diagnosis. Furthermore, they suggest the existence of novel therapeutic approaches targeting chromosomal alterations, mutated genes and altered signaling pathways, methylation changes, microRNAs, and immune checkpoints. Moving forward, further research is imperative to explore the pathogenesis of CNS GCTs and unravel the intricate interactions among various molecular alterations. Additionally, these findings require validation in clinical cohorts to assess their role in the diagnosis, risk stratification, and treatment of patients. Full article

Background: Relapse of Candida albicans urinary tract infection (UTI) is frequent despite appropriate treatment, as commonly used antifungals such fluconazole and flucytosine are only fungistatics. To improve treatment of Candida UTI and decrease relapses, understanding the long-term metabolic activity and survival of C. albicans in urine containing antifungals at minimal inhibitory concentration (MIC) is needed. Methods: we monitored the survival, metabolic activity and consumption of glucose and proteins by C. albicans using conventional methods and isothermal microcalorimetry (IMC). We also investigated the influence of dead Candida cells on the growth of their living counterparts. Results: For 33 days, weak activity was observed in samples containing antifungals in which C. albicans growth rate was reduced by 48%, 60% and 88%, and the lag increased to 172 h, 168 h and 6 h for amphotericin, flucytosine and fluconazole, respectively. The metabolic activity peaks corresponded to the plate counts but were delayed compared to the exhaustion of resources. The presence of dead cells promoted growth in artificial urine, increasing growth rate and reducing lag in similar proportions. Conclusions: Even with antifungal treatment, C. albicans relapses are possible. The low metabolic activity of surviving cells leading to regrowth and chlamydospore formation possibly supported by autophagy are likely important factors in relapses. Full article

The global iron ore price is influenced by numerous factors, thus showcasing a complex interplay among them. The collective expectations of market participants over time shape the variations and trends within the iron ore price time series. Consequently, devising a robust forecasting model for the volatility of iron ore prices, as well as for other assets connected to this commodity, is critical for guiding future investments and decision-making processes in mining companies. Within this framework, the integration of artificial intelligence techniques, encompassing both technical and fundamental analyses, is aimed at developing a comprehensive, autonomous hybrid system for decision support, which is specialized in iron ore asset management. This approach not only enhances the accuracy of predictions but also supports strategic planning in the mining sector. Full article

Rheumatoid arthritis (RA) is a chronic inflammatory disease. Despite new methods of diagnostics and treatment as well as extensive biological and immunosuppressive treatment, the etiology of RA is not fully understood. Moreover, the problem of diagnosis and treatment of RA patients is still current and affects a large group of patients. It is suggested that endoplasmic reticulum (ER)-related features may impair adaptation to chronic stress, inferring the risk of rheumatoid arthritis. The main goal in this study was evaluation of changes in mRNA translation to determine chronic ER stress conditions in rheumatoid arthritis patients. The study group consist of 86 individuals including a total of 56 rheumatoid arthritis patients and 30 healthy controls. The expression level of mRNA form blood samples of RA patients as well as controls of the unfolded protein response (UPR)-associated genes (p-eIF2, BCL-2, PERK, ATF4, and BAX) were investigated using real-time qPCR. GAPDH expression was used as a standard control. Considering the median, the expression levels of PERK, BCL-2, p-eIF2, ATF4, and BAX were found to be significantly increased in the blood of RA patients compared with the control group. The p-value for the PERK gene was 0.0000000036, the p-value for the BCL-2 gene was 0.000000014, the p-value for the p-eIF2 gene was 0.006948, the p-value for the ATF4 gene was 0.0000056, and the p-value for the BAX gene was 0.00019, respectively. Thus, it can be concluded that the targeting of the components of the PERK-dependent UPR signaling pathway via small-molecule PERK inhibitors may contribute to the development of novel, innovative treatment strategies against rheumatoid arthritis. Full article