In the present study, we conducted a scoping review to provide an overview of the existing literature on the carbocyanine dye DiI, in human neuroanatomical tract tracing. The PubMed, Scopus, and Web of Science databases were systematically searched. We identified 61 studies published during the last three decades. While studies incorporated specimens across human life from the embryonic stage onwards, the majority of studies focused on adult human tissue. Studies that utilized peripheral nervous system (PNS) tissue were a minority, with the majority of studies focusing on the central nervous system (CNS). The most common topic of interest in previous tract tracing investigations was the connectivity of the visual pathway. DiI crystals were more commonly applied. Nevertheless, several studies utilized DiI in a paste or dissolved form. The maximum tracing distance and tracing speed achieved was, respectively, 70 mm and 1 mm/h. We identified studies that focused on optimizing tracing efficacy by varying parameters such as fixation, incubation temperature, dye re-application, or the application of electric fields. Additional studies aimed at broadening the scope of DiI use by assessing the utility of archival tissue and compatibility of tissue clearing in DiI applications. A combination of DiI tracing and immunohistochemistry in double-labeling studies have been shown to provide the means for assessing connectivity of phenotypically defined human CNS and PNS neuronal populations. Full article

Chronic Hepatitis B and D Virus (HBV and HDV) co-infection is responsible for the most severe form of viral Hepatitis, the Hepatitis Delta. Despite an efficient vaccine against HBV, the HBV/HDV infection remains a global health burden. Notably, no efficient curative treatment exists against any of these viruses. While physiologically distinct, HBV and HDV life cycles are closely linked. HDV is a deficient virus that relies on HBV to fulfil is viral cycle. As a result, the cellular response to HDV also influences HBV replication. In vitro studying of HBV and HDV infection and co-infection rely on various cell culture models that differ greatly in terms of biological relevance and amenability to classical virology experiments. Here, we review the various cell culture models available to scientists to decipher HBV and HDV virology and host–pathogen interactions. We discuss their relevance and how they may help address the remaining questions, with one objective in mind: the development of new therapeutic approaches allowing viral clearance in patients. Full article

The influence of chloride ion (Cl⁻) concentration on the corrosion mechanism of WC–MgO composites has been studied in this work. The results suggest that the corrosion resistance of WC–MgO composite decreases first and then increases with the increase in Cl⁻ concentration. Solution conductivity and dissolved oxygen content are the main influence factors. The solution conductivity determines the charge transfer process, and the dissolved oxygen determines the cathodic oxygen absorption reaction. The corrosion characteristic is typical pitting corrosion. Meanwhile, the corrosion mechanism contains an oxidation process of the WC matrix and the dissolution destruction of the MgO toughening phase. The formation of the WO₃ corrosion layer hinders the general corrosion to protect the inner material. However, the dissolution of MgO induces the initiation of pitting. The local alkaline caused by MgO dissolution promotes the dissolution of the WC matrix, which leads to the expansion of pitting. Full article

Acute pancreatitis (AP) is a common but often self-limiting disease in the majority of patients. However, in the minority, who may progress to moderately severe or severe AP, high mortality risk has been reported. Infected pancreatitis necrosis (IPN) in necrotising pancreatitis has been shown to result in more than twice the mortality rate compared with in sterile pancreatic necrosis. This raises the question on whether prophylactic antibiotics (PABs) should be given in subgroups of AP to prevent superimposed infection to improve survival outcomes. Despite numerous randomised controlled trials (RCTs), meta-analyses, and guidelines on the management of AP, there is a lack of strong evidence to suggest the use of PABs in AP. Additionally, use of PABs is associated with antimicrobial resistance. Considerable heterogeneity exists and limits the interpretation of results—subgroup of AP benefitting from PAB use, choice/class of PAB, and timing of administration from symptom onset and duration of PAB use. Only a minority of existing meta-analyses suggest mortality benefits and reduction in IPN. The majority of existing guidelines do not recommend the use of PABs in AP. More research is required to make more definitive conclusions. Currently, PAB should only be administered after multidisciplinary discussions led by pancreatology experts. Full article

Deriving from the growing cultural and religious diversity in Germany and the need for educational professionals to be able to deal with heterogeneous groups and communicate to children about how to coexist peacefully with others, this essay focuses on the relationship between common sense and tolerance, particularly in places of inescapable dense coexistence characterised by religious and cultural diversity. Using institutions of preschool and primary education as an example, the extent to which peaceful coexistence and conflict resolution is borne by common sense and supported by practices of tolerance is discussed. Subsequently, the significance of a common-sense approach to practices of tolerance for conceptualising ethical judgement in intercultural and inter-religious education is explored. The article closes with thoughts on the contribution of religious ethics to questions of human coexistence in highly dense and conflict-ridden contexts and briefly addresses aspects of Protestant social ethics. Full article

The pricing behavior of agricultural processing firms in input markets has large impacts on farmers’ and processors’ prosperity as well as the overall market structure. Despite analytical approaches to food processors’ pricing in agricultural input markets, the need for models to represent complex market features is urgent. Agent-based models (ABMs) serve as computational laboratories to understand complex markets emerging from autonomously interacting agents. Yet, individual agents within ABMs must be equipped with intelligent learning algorithms. In this paper, we propose supervised and unsupervised learning agents to simulate the pricing behavior of firms in agricultural markets’ ABMs. Supervised learning firms are pre-trained to accurately best respond to their competitors and are deemed to result in the market Nash equilibria. Unsupervised learning firms play a course of pricing interaction with their competitors without any pre-knowledge but based on deep reinforcement learning. The simulation results show that unsupervised deep learning firms are capable of approximating the pricing equilibria obtained by the supervised firms in different spatial market settings. Optimal discriminatory and uniform delivery pricing emerges in agricultural input markets with the high and intermediary importance placed on space. Free on board pricing emerges in agricultural input markets with small importance placed on space. Full article

Mission statement components are crucial for the sustainability of social enterprises. Based on content analysis and fuzzy-set qualitative comparative analysis (fsQCA), we investigate the link between mission statement components and sustainability in a sample of 148 Chinese social enterprises. Our findings indicate that social enterprises’ mission statements are both socially and commercially focused. An individual mission statement component does not constitute a necessary condition for the high sustainability of social enterprises, but products or services and enterprise philosophy play a universal role in this regard. There are four configurations of mission statement components that lead to high social enterprise sustainability. This study helps to reveal the complexity of the impact of mission statement components on social enterprise sustainability. Full article

We introduce a compact core model for double-gate (DGFET) and surrounding-gate (SGFET) MOSFETs designed for circuit simulations. Despite its high precision, the model is crafted to retain the same analytic formulation of the industry standard Pennsylvania State and Philips (PSP). Instead of linearizing the drain current as in the PSP model, we employ a quadratic symmetric polynomial interpolation of the charge in the channel. This eliminates the need for cumbersome derivatives, simplifications, and intricate coding when integrating into a circuit simulator, thereby preventing singularities during numerical iterations. Moreover, thanks to its mathematical formulation equivalent to PSP, this model simplifies the coding of terminal charges, capacitances, potentials, and electric fields in the channel within circuit simulators. We validate the accuracy of the model through comparisons with numerical solutions and experiments from the literature. Full article

The demand for affordable prostheses, particularly in low- and middle-income countries (LMICs), is significant. Currently, the majority of prosthetic sockets are manufactured using monolithic thermoplastic polymers such as PP (polypropylene), which lack durability, strength, and exhibit creep. Alternatively, they are reinforced with consumptive thermoset resin and expensive composite fillers such as carbon, glass, or Kevlar fibres. However, there are unmet needs that amputees face in obtaining affordable prosthetic sockets, demanding a solution. This study utilises self-reinforced PET (polyethylene terephthalate), an affordable and sustainable composite material, to produce custom-made sockets. Advancing the development of a unique socket manufacturing technique employing a reusable vacuum bag and a purpose-built curing oven, we tested fabricated sockets for maximum strength. Subsequently, a prosthetic device was created and assessed for its performance during ambulation. The mechanical and structural strength of PET materials for sockets reached a maximum strength of 132 MPa and 5686 N. Findings indicate that the material has the potential to serve as a viable substitute for manufacturing functional sockets. Additionally, TOPSIS analysis was conducted to compare the performance index of sockets, considering decision criteria such as material cost, socket weight, and strength. The results showed that PET sockets outperformed other materials in affordability, durability, and strength. The methodology successfully fabricated complex-shaped patient sockets in under two hours. Additionally, walking tests demonstrated that amputees could perform daily activities without interruptions. This research makes significant progress towards realising affordable prostheses for LMICs, aiming to provide patient-specific affordable prostheses tailored for LMICs. Full article

To reduce pesticide pollution and promote sustainable agricultural development in China, we designed a pilot-scale biofilter system to treat residual imidacloprid wastewater in an orchard. The biofilter system demonstrated a high rate of removal of imidacloprid from the biodegradation wastewater, with removal rates from the outlet exceeding 99% at different concentrations of pesticides. Among environmental factors, imidacloprid concentration at the inlet and biomixture significantly affected the activity of imidacloprid-degrading bacteria. The dominant microbial communities during the stable operation of the biofilter system included Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes at the phylum level and Bacillus, Methylobacter, and unclassified_f__Microbacteriaceae at the genus level. In future initiatives to improve biofilter performance and applicability, increasing attention should be paid to the dominant microbial communities, the number of biofilter units, and important environmental factors. Orchard workers in China should improve the existing treatment of residual pesticide wastewater to mitigate agricultural non-point source pollution. Full article

2, 6-diisopropylaniline (2, 6-DIPA) is a crucial non-intentionally organic additive that allows the assessment of the production processes, formulation qualities, and performance variations in biodegradable mulching film. Moreover, its release into the environment may have certain effects on human health. Hence, this study developed simultaneous heating hydrolysis–extraction and amine switchable hydrophilic solvent vortex-assisted homogeneous liquid–liquid microextraction for the gas chromatography–mass spectrometry analysis of the 2, 6-DIPA additive and its corresponding isocyanates in poly(butylene adipate-co-terephthalate) (PBAT) biodegradable agricultural mulching films. The heating hydrolysis–extraction conditions and factors influencing the efficiency of homogeneous liquid–liquid microextraction, such as the type and volume of amine, homogeneous-phase and phase separation transition pH, and extraction time were investigated and optimized. The optimum heating hydrolysis–extraction conditions were found to be a H₂SO₄ concentration of 2.5 M, heating temperature of 87.8 °C, and hydrolysis–extraction time of 3.0 h. As a switchable hydrophilic solvent, dipropylamine does not require a dispersant. Vortex assistance is helpful to speed up the extraction. Under the optimum experimental conditions, this method exhibits a better linearity (0.0144~7.200 μg mL⁻¹ with R = 0.9986), low limit of detection and quantification (0.0033 μg g⁻¹ and 0.0103 μg g⁻¹), high extraction recovery (92.5~105.4%), desirable intra- and inter-day precision (relative standard deviation less than 4.1% and 4.7%), and high enrichment factor (90.9). Finally, this method was successfully applied to detect the content of the additive 2, 6-DIPA in PBAT biodegradable agricultural mulching films, thus facilitating production process monitoring or safety assessments. Full article

This paper proposes an asymmetric hybrid tri-stable piezoelectric energy harvester for rotational motion (RHTPEH). The device features an asymmetric tri-stable piezoelectric cantilever beam positioned at the edge of a rotating disk. This beam is uniquely configured with an asymmetric arrangement of magnets. Additionally, an elastic amplifier composed of a vertical and a rotating spring connects the beam’s fixed end and the disk. This setup enhances both the rotational amplitude and vertical displacement of the beam during motion. A comprehensive dynamical model of the RHTPEH was developed using Lagrange’s equations. This model facilitated an in-depth analysis of the system’s behavior under various conditions, focusing on the influence of key parameters such as the asymmetry in the potential well, the stiffness ratio of the amplifier springs, the radius of the disk, and the disk’s rotational speed on the nonlinear dynamic response of the system. The results show that the asymmetric hybrid tri-stable piezoelectric energy harvester makes it easier to harvest the vibration energy in rotational motion and has excellent power output performance compared with the symmetric tri-stable piezoelectric energy harvester. The output power magnitude of the system at higher rotational speeds increases as the radius of rotation expands, but when the rotational speed is low, the steady-state output power magnitude of the system is not sensitive to changes in the radius of rotation. Theoretical analysis and numerical simulations validate the effectiveness of the proposed asymmetric RHTPEH for energy harvesting in low-frequency rotating environments. Full article

Frequent and prolonged exposure to high levels of vibration and shock can cause neck and back pain and discomfort for many wheelchair users. Current methods to attenuate the vibration have shown to be ineffective and, in some cases, detrimental to health. Novel in-wheel suspension systems claim to offer a solution by replacing traditional spokes of the rear wheels with dampening elements or springs. The objective of this study was to investigate the effects of in-wheel suspension on reducing vibration and shock and improving comfort in manual wheelchair users. Twenty-four manual wheelchair users were propelled over nine different surfaces using a standard spoked wheel, a Spinergy CLX, and Loopwheels while accelerometry data was collected at the footrest, seat, and backrest. Loopwheels lowered vibrations by 10% at the backrest compared to the standard and CLX wheels (p-value < 0.001) and by 7% at the footrest compared to the CLX (p-value < 0.05). They also reduced shocks by 7% at the backrest compared to the standard wheel and CLX (p-value < 0.001). No significant differences were found in comfort between the wheels. Results indicate that Loopwheels is effective at reducing vibration and shock, but more long-term testing is required to determine effects on health. Full article

Early childhood caries (ECC) is a growing public health concern worldwide. Although numerous systematic reviews have been published regarding the association between breastfeeding and early childhood caries (ECC), the results remain inconclusive and equivocal. This systematic review synthesises the evidence on the association between breastfeeding and ECC. Five electronic databases and backward citation chasing were performed from inception until May 2023. A total of 31 studies (22 cohort studies and 9 case-control studies) were included in this review. The meta-analysis of the case-control studies showed statistically significant fewer dental caries in children who were breastfed for < 6 months compared to those who were breastfed for ≥6 months (OR = 0.53, 95% CI 0.41–0.67, p < 0.001). There was a statistically significant difference in dental caries between children who were breastfed for <12 months and those who were breastfed for ≥12 months (RR = 0.65, 95% CI 0.50–0.86, p < 0.002). Similarly, there was a statistically significant difference in dental caries in children who were breastfed for < 18 months compared to those who were breastfed for ≥18 months (RR = 0.41, 95% CI 0.18–0.92, p = 0.030). Nocturnal breastfeeding increases the risk of ECC compared with no nocturnal breastfeeding (RR = 2.35, 95% CI 1.42–3.89, p < 0.001). The findings suggest breastfeeding for more than 12 months and nocturnal breastfeeding increase the risk of ECC. Full article

As a recently developed high-strength aluminium alloy used specifically for laser additive manufacturing, AlMgMnSc alloy possesses superior mechanical properties and excellent processability. Extreme high-speed laser deposition (EHLD) is a novel surface-modification technique, which is characterised by high depositing speed, rapid cooling, rate and minimal dilution rate. To offer a new method for surface repairing high-strength aluminium alloys, an AlMgMnSc alloy coating, containing two deposition layers, is prepared on a 6061 aluminium-alloy axle using the EHLD technique. Meanwhile, the microstructure, composition distribution, and microhardness variation of the fabricated coating are studied. The results reveal that the coating is dense and crack-free, which is well-bonded with the substrate. Additionally, layer 1 is mainly composed of large columnar and equiaxed grains, while layer 2 consists of a fully equiaxed grain structure with an average grain size of about 4.5 μm. Moreover, the microhardness of the coating (about 104~118 HV) is similar to the substrate (about 105 HV), proving the feasibility of repairing high-strength aluminium alloys using AlMgMnSc alloy powders through the EHLD technique. Full article

This study optimized the input processing factors, namely compression force, pressing speed, heating temperature, and heating time, for extracting oil from desiccated coconut medium using a vertical compression process by applying a maximum load of 100 kN. The samples’ pressing height of 100 mm was measured using a vessel chamber of diameter 60 mm with a plunger. The Box–Behnken design was used to generate the factors’ combinations of 27 experimental runs with each input factor set at three levels. The response surface regression technique was used to determine the optimum input factors of the calculated responses: oil yield (%), oil expression efficiency (%), and energy (J). The optimum factors’ levels were the compression force 65 kN, pressing speed 5 mm min⁻¹, heating temperature 80 °C, and heating time 52.5 min. The predicted values of the responses were 48.48%, 78.35%, and 749.58 J. These values were validated based on additional experiments producing 48.18 ± 0.45%, 77.86 ± 0.72%, and 731.36 ± 8.04 J. The percentage error values between the experimental and the predicted values ranged from 0.82 ± 0.65 to 2.43 ± 1.07%, confirming the suitability of the established regression models for estimating the responses. Full article

This paper presents an intelligent system for the dynamic estimation of sheep body weight (BW). The methodology used to estimate body weight is based on measuring seven biometric parameters: height at withers, rump height, body length, body diagonal length, total body length, semicircumference of the abdomen, and semicircumference of the girth. A biometric parameter acquisition system was developed using a Kinect as a sensor. The results were contrasted with measurements obtained manually with a flexometer. The comparison gives an average root mean square error (RMSE) of 9.91 and a mean $R^2$ of 0.81. Subsequently, the parameters were used as input in a back-propagation artificial neural network. Performance tests were performed with different combinations to make the best choice of architecture. In this way, an intelligent body weight estimation system was obtained from biometric parameters, with a 5.8% RMSE in the weight estimations for the best architecture. This approach represents an innovative, feasible, and economical alternative to contribute to decision-making in livestock production systems. Full article

Alkali-activated cementitious materials, as an environmentally friendly cementitious material, can effectively reduce carbon emissions and improve the utilisation of solid wastes. However, traditional strong alkali activators have limitations such as high carbon emissions and poor safety. In order to overcome the defects of traditional strong alkaline activators and realise the high value-added use of calcium carbide residue (CCR), this paper adopts CCR as an alkaline activator to activate granulated blast furnace slag (GBFS)-steel slag (SS) cementitious systems for the preparation of alkaline-activated cementitious materials. The effects of CCR content and SS content on the compressive strength and working performance of CCR-GBFS-SS cementitious systems are analysed, along with the hydration process of CCR-GBFS-SS cementitious systems and the mechanism of action through the hydration products, their chemical structure and their microscopic morphology. The research results show that CCR-GBFS-SS cementitious systems have a 28-day compressive strength of 41.5 MPa and they can be controlled by the setting time; however, the flow performance is poor. The SS content can be increased to improve the flow performance; however, this will reduce the compressive strength. In CCR-GBFS-SS cementitious systems, CCR is the main driving force of hydration reactions, GBFS mainly provides active silica and aluminium and the amorphous C-(A)-S-H gel and ettringite formed by the synergistic action of multiple solid wastes are the main sources of compressive strength. With the extension of the curing time, the amount of hydration products in the cementitious systems gradually increases and the matrix of the cementitious systems gradually becomes denser. This study will provide a reference for the consumption of low-value solid waste such as CCR and the preparation of low-carbon cementitious materials from multi-component solid wastes. Full article

Fibromyalgia syndrome (FM) is a chronic pain condition that affects a significant portion of the population; yet, this condition is still poorly understood. Prior research has suggested that individuals with FM display a heightened sensitivity to pain and signs of autonomic dysfunction. Recent advances in functional MRI analysis methods to model blood-oxygenation-level-dependent (BOLD) responses across networks of regions, and structural and physiological modeling (SAPM) have shown the potential to provide more detailed information about altered neural activity than was previously possible. Therefore, this study aimed to apply novel analysis methods to investigate altered neural processes underlying pain sensitivity in FM in functional magnetic resonance imaging (fMRI) data from the brainstem and spinal cord. Prior fMRI studies have shown evidence of functional differences in fibromyalgia (FM) within brain regions associated with pain’s motivational aspects, as well as differences in neural activity related to pain regulation, arousal, and autonomic homeostatic regulation within the brainstem and spinal cord regions. We, therefore, hypothesized that nociceptive processing is altered in FM compared to healthy controls (HCs) in the brainstem and spinal cord areas linked to autonomic function and descending pain regulation, including the parabrachial nuclei (PBN) and nucleus tractus solitarius (NTS). We expected that new details of this altered neural signaling would be revealed with SAPM. The results provide new evidence of altered neural signaling in FM related to arousal and autonomic homeostatic regulation. This further advances our understanding of the altered neural processing that occurs in women with FM. Full article

This research utilized long short-term memory (LSTM) to oversee an RLS-based mass estimator based on longitudinal vehicle dynamics for heavy-duty vehicles (HDVs) instead of using the predefined rules. A multilayer LSTM network that analyzed parameters such as vehicle speed, longitudinal acceleration, engine torque, engine speed, and estimated mass from the RLS mass estimator was employed as the supervision method. The supervisory LSTM network was trained offline to recognize when the vehicle was operated so that the RLS estimator gave an estimate with the desired accuracy and the network was used as a reliability flag. High-fidelity simulation software was employed to collect data used to train and test the network. A threshold on the error percentage of the RLS mass estimator was used by the network to check the reliability of the algorithm. The preliminary findings indicate that the reliability of the RLS mass estimator could be predicted by using the LSTM network. Full article

The treatment of metastatic melanoma has been revolutionised by immunotherapy, yet a significant number of patients do not respond, and many experience autoimmune adverse events. Associations have been reported between patient outcome and monocyte subsets, whereas vitamin C (ascorbate) has been shown to mediate changes in cancer-stimulated monocytes in vitro. We therefore investigated the relationship of ascorbate with monocyte subsets and epigenetic modifications in patients with metastatic melanoma receiving immunotherapy. Patients receiving immunotherapy were compared to other cancer cohorts and age-matched healthy controls. Ascorbate levels in plasma and peripheral blood-derived mononuclear cells (PBMCs), monocyte subtype and epigenetic markers were measured, and adverse events, tumour response and survival were recorded. A quarter of the immunotherapy cohort had hypovitaminosis C, with plasma and PBMC ascorbate levels significantly lower than those from other cancer patients or healthy controls. PBMCs from the immunotherapy cohort contained similar frequencies of non-classical and classical monocytes. DNA methylation markers and intracellular ascorbate concentration were correlated with monocyte subset frequency in healthy controls, but correlation was lost in immunotherapy patients. No associations between ascorbate status and immune-related adverse events or tumour response or overall survival were apparent. Full article

In the inquisitorial archive of Pedro Onofre Cortés, alias Moixina, we see fellow practitioner protesting his son’s marriage to Clara Sureda because she was an Old Christian. The poor match was blamed on the breast milk that was ingested as an infant, “andaba con cristianos porque había mamado leche de una mujer cristiana” (he went with Christians because of the milk drunk milk from a Christian woman) (Picazo y Muntaner). In early modern Spain, breastmilk was seen as responsible for transmitting virtues and vices, religious expressions of faith and moral traits. Following Galenic medical understanding equating milk with blood, it was women who were responsible for the transmission of purity, impurity (Alexandre-Bidon 175), for contamination and difference (Martínez 47). This brief citation reflects the hybrid environment and the dual practices that deeply informed the lives of the converso Jews. Moreover, the understanding of the hereditary nature of these traits, and the traditions of Judaism and Christianity, so often mixed in unique combinations are clearly demonstrated in the Inquisition trials of Cortés and his Chueta brethren. As regulation over the mother and the female body became increasingly important in controlling Iberian subjects and its empire, conversos complicate the feminization of impurity. This article explores how the conversos known as the Chuetas of Mallorca understood their religiosity and difference as seen through the lens of hybridity, breast milk and maternal care. Full article