Background: Implanted titanium skull plates (TSPs) in cranioplasty are used to replace or reconstruct areas of the skull that have been damaged or removed due to trauma, surgery, or other medical conditions. However, the presence of a TSP in the head may influence the distribution of the electric field induced during transcranial magnetic stimulation (TMS) procedures. The purpose of this study was to determine how the presence of TSP would interfere with TMS-induced cortical electric fields. Methods: The TMS with a figure-of-eight coil was applied to a realistic head model with TSPs. The distribution of the induced electric field in head tissues was calculated by employing the impedance method, and the results were compared with that of a normal head without TSP. Results: Simulation results show that the distribution of the induced electric field has changed greatly for the head model with TSP. The maximum value of the induced electric field in head tissues was present under one of the circular coil wings rather than in the tissues beneath the junction of the two wings of the Fo8 coil. Conclusions: The induced electric field in deep brain regions was increased for the head model with TSP, which could potentially lead to deep brain stimulation. Since the presence of metallic TSP can greatly influence the distribution of the induced electric field in TMS applications, it is important to adjust the treatment scheme when considering TMS for individuals with cranial titanium plates. Full article

In order to ensure that aircraft have medium and long-range flights, enhanced aerodynamic performance, and reduced fuel consumption, this paper presents an original Distributed Hybrid Electric Propulsion Aircraft (DHEPA) design scheme and proposes a novel power allocation management method based on convex optimization. Firstly, by taking the Tecnam P2006T general-purpose aircraft as a reference, key components of DHEPA are selected and modeled. Then, a power allocation management method for DHEPA is proposed on the basis of convex optimization, which takes the minimum fuel consumption as the performance index to realize the reasonable power allocation of the battery and engine, while avoiding sliding into the local optimum of allocation. Finally, momentum theory and numerical simulation methods are used to analyze the aerodynamic enhancement effect of the propeller on the wing in the DHEPA, and a dynamics method is utilized to calculate the dynamics performance of the aircraft at several important stages. The results show that, compared with the reference aircraft, the lift of the DHEPA is increased by 46%. Under typical sectors, the DHEPA has a higher rate of climb and maximum leveling off speed at cruise, and a significantly lower fuel consumption. Full article

Loop-mediated isothermal amplification (LAMP) technology is extensively utilized for the detection of infectious diseases owing to its rapid processing and high sensitivity. Nevertheless, conventional LAMP signaling methods frequently suffer from a lack of sequence specificity. This study integrates a triplex-forming oligonucleotide (TFO) probe into the LAMP process to enhance sequence specificity. This TFO-LAMP technique was applied for the detection of Group B Streptococcus (GBS). The TFO probe is designed to recognize a specific DNA sequence, termed the TFO targeting sequence (TTS), within the amplified product, facilitating detection via fluorescent instrumentation or lateral flow biosensors. A screening method was developed to identify TFO sequences with high affinity to integrate TFO into LAMP, subsequently incorporating a selected TTS into an LAMP primer. In the TFO-LAMP assay, a FAM-labeled TFO is added to target the TTS. This TFO can be captured by an anti-FAM antibody on lateral flow test strips, thus creating a nucleic acid testing biosensor. The efficacy of the TFO-LAMP assay was confirmed through experiments with specimens spiked with varying concentrations of GBS, demonstrating 85% sensitivity at 300 copies and 100% sensitivity at 30,000 copies. In conclusion, this study has successfully developed a TFO-LAMP technology that offers applicability in lateral flow biosensors and potentially other biosensor platforms. Full article

The difficulties of offline education caused by the COVID-19 epidemic are attracting increasing public attention. Although international research on online education platforms is gradually deepening, the research on the influencing factors of Chinese users’ participation in these platforms shows different results due to regional differences. Therefore, this study adopts the technology acceptance model (TAM) as the basis to build the index system of influencing factors for the Tencent conference online education platform. The questionnaire design covered five dimensions, including external environment, platform satisfaction, and continuous use intention. An online questionnaire survey was conducted on participants in some regions of China, 320 questionnaires were distributed, and 297 valid questionnaires were collected. The data were analyzed by SPSS 26.0, and the results of Cronbach’s coefficient of 0.939 and a KMO value of 0.941 were obtained. The weight value, entropy value, and importance ranking of the factors were determined by combining hierarchical analysis and the entropy weighting method. First, the perceived ease of use is the most critical factor influencing the users’ use of online delivery platforms. Second, freedom from geographical time difference, the ease of operation, system perfection, and proficiency in use greatly influenced the participants’ use of the online lecture platform. Third, the study group suggested that the platform should be optimized in terms of convenience, stability, and freedom from geographical time difference, and provide reasonable references and lessons for future educational efforts. Full article

Clinical syndromes associated with antibodies against myelin oligodendrocyte glycoprotein (MOG) are now recognized as a distinct neurological disease entity, and are gaining increasing attention. The pathogenic mechanisms underlying MOG-antibody disease (MOGAD) remain incompletely understood. Case series, facilitated by registries, and observational studies over the past few years have shed increasing light on the clinical aspects and therapeutic approaches of MOGAD. MOGAD may manifest with a variety of clinical syndromes, including acute disseminated encephalomyelitis (ADEM), autoimmune encephalitis, optic neuritis (ON) and transverse myelitis (TM). MOGAD can be either monophasic or relapsing. This review aims to provide a comprehensive updated description of the clinical spectrum, paraclinical features, and prognosis of MOG-antibody disease, as well as summarize its therapeutic considerations. Randomized clinical trials, standardized diagnostic criteria and treatment guidelines are the steps forward. Full article

The benefits of urban trees are very well known, but they can fall and cause damage, putting people’s lives at risk. There are few studies on the vulnerability of species to falling. In Lisbon (Portugal), fallen trees have been recorded since 1990 without, however, the identification of the species, knowledge of which is fundamental for improving their management. This study aimed to identify the tree species most vulnerable to falling in Lisbon through a proximity-based approach of known species, since the city has 47,713 inventoried trees, of which only 26,595 (55.7%) were identified. Four criteria were designed to presume the species: (i) the tree must be within 15 m from the street median axis; (ii) at least three individuals within 30 m from the occurrence must belong to the same species; (iii) the surrounding species must be representative in the street (>50%); and (iv) visual identification of avenue medians. Through this approach, considering 3767 fallen trees, it was possible to identify 736 cases, representing 19.5% of all occurrences throughout the studied time and representing 43 different species. Species like Morus nigra L., Tipuana tipu (Benth.) Kuntze, Liriodendron tulipifera L., Prunus cerasifera Ehrh., and Koelreuteria paniculata Laxm. were most vulnerable. Additionally, in 57.7% of cases (425 fallen trees), the wind speed 12-h before the occurrence was greater than 7 m s⁻¹. This research will provide important data for urban planners seeking to maximize the ecosystem services of urban trees. Full article

The possibility of detecting the developmental competence of individually cultured embryos through analysis of spent media is a major current trend in an ART setting. However, individual embryo culture is detrimental compared with high-density group culture due to the reduced concentration of putative embryotropins. The main aim of this study was to identify an individual culture system that is not detrimental over high-density group culture in the bovine model. Blastocyst rates and competence were investigated in a conventional (GC) group, semi-confined group (MG), and individual culture (MS) in a commercial microwell device. Main findings showed that: (1) individual embryos can be continuously cultured for 7 days in ~70 nL microwells (MS) without detrimental effects compared with the GC and MG; (2) MS and MG blastocysts had a reduced number of TUNEL-positive cells compared to GC blastocysts; (3) though blastocyst mean cell numbers, mitochondrial activity, and lipid content were not different among the three culture conditions, MS blastocysts had a higher frequency of small-sized lipid droplets and a reduced mean droplet diameter compared with GC and MG blastocysts. Overall, findings open the way to optimize the development and competence of single embryos in an ART setting. Full article

The development of photocurable compositions is in high demand for the manufacture of functional materials for electronics, optics, medicine, energy, etc. The properties of the final photo-cured material are primarily determined by the initial mixture, which needs to be tuned for each application. In this study we propose to use simple systems based on di(meth)acrylate, polyimide and photoinitiator for the preparation of new photo-curable compositions. It was established that a fluorinated cardo copolyimide (FCPI) based on 2,2-bis-(3,4-dicarboxydiphenyl)hexafluoropropane dianhydride, 9,9-bis-(4-aminophenyl)fluorene and 2,2-bis-(4-aminophenyl)hexafluoropropane (1.00:0.75:0.25 mol) has excellent solubility in di(met)acrylates. This made it possible to prepare solutions of FCPI in such monomers, to study the effect of FCPI on the kinetics of their photopolymerization in situ and the properties of the resulting polymers. According to the obtained data, the solutions of FCPI (23 wt.%) in 1,4-butanediol diacrylate (BDDA) and FCPI (15 wt.%) in tetraethylene glycol diacrylate were tested for the formation of the primary protective coatings of the silica optical fibers. It was found that the new coating of poly(BDDA–FCPI_23%) can withstand prolonged annealing at 200 °C (72 h), which is comparable or superior to the known most thermally stable photo-curable coatings. The proposed approach can be applied to obtain other functional materials. Full article

Tea plants (Camellia sinensis (L.) Kuntze) are a cash crop that thrive under warm and moist conditions. However, tea plants are becoming increasingly vulnerable to heat damage (HD) during summer growing seasons due to global climate warming. Because China ranks first in the world in both harvested tea area and total tea production, monitoring and tracking HD to tea plants in a timely manner has become a significant and urgent task for scientists and tea producers in China. In this study, the spatiotemporal characteristics of HD evolution were analyzed, and a tracking method using HD LST-weighted geographical centroids was constructed based on HD pixels identified by the critical LST threshold and daytime MYD11A1 products over the major tea planting regions of mainland China from two typical HD years (2013 and 2022). Results showed that the average number of HD days in 2022 was five more than in 2013. Daily HD extent increased at a rate of 0.66% per day in 2022, which was faster than that in 2013 with a rate of 0.21% per day. In two typical HD years, the tea regions with the greatest HD extent were concentrated south of the Yangtze River (SYR), with average HD pixel ratios of greater than 50%, then north of the Yangtze River (NYR) and southwest China (SWC), with average HD pixel ratios of around 40%. The regions with the least HD extent were in South China (SC), where the HD ratios were less than 40%. The HD LST-weighted geographical centroid trajectories showed that HD to tea plants in 2013 initially moved from southwest to northeast, and then moved west. In 2022, HD moved from northeast to west and south. Daily HD centroids were mainly concentrated at the conjunction of SYR, SWC, and SC in 2013, and in northern SWC in 2022, where they were near to the centroid of the tea planting gardens. The findings in this study confirmed that monitoring HD evolution of tea plants over a large spatial extent based on reconstructed remotely sensed LST values and critical threshold was an effective method benefiting from available MODIS LST products. Moreover, this method can identify and track the spatial distribution characteristics of HD to tea plants in a timely manner, and it will therefore be helpful for taking effective preventative measures to mitigate economic losses resulting from HD. Full article

The rapid urbanization of Abha and its surrounding cities in Saudi Arabia’s mountainous regions poses challenges for sustainable and secure development. This study aimed to identify suitable sites for eco-friendly and safe building complexes amidst complex geophysical, geoecological, and socio-economic factors, integrating natural hazards assessment and risk management. Employing the Fuzzy Analytic Hierarchy Process (Fuzzy-AHP), the study constructed a suitability model incorporating sixteen parameters. Additionally, a Deep Neural Network (DNN) based on eXplainable Artificial Intelligence (XAI) conducted sensitivity analyses to assess the parameters’ influence on optimal location decision making. The results reveal slope as the most crucial parameter (22.90%), followed by altitude and land use/land cover (13.24%), emphasizing topography and environmental considerations. Drainage density (11.36%) and rainfall patterns (9.15%) are also significant for flood defense and water management. Only 12.21% of the study area is deemed “highly suitable”, with “no-build zones” designated for safety and environmental protection. DNN-based XAI demonstrates the positive impact of variables like the NDVI and municipal solid waste generation on site selection, informing waste management and ecological preservation strategies. This integrated methodology provides actionable insights for sustainable and safe residential development in Abha, aiding informed decision making and balancing urban expansion with environmental conservation and hazard risk reduction. Full article

Twelve dogs with oral malignant melanomas (MM) were evaluated in this study, with demographic details indicating a balanced distribution of gender, age, and weight among various breeds. Tumor locations varied, with diverse surgical procedures being performed, including mandibulectomies and maxillectomies. Lymphadenectomies were conducted, revealing a 16.66% metastatic rate in regional lymph nodes. At the time of surgery, clinical staging identified stages I, II, and III, with most cases having non-infiltrated margins and a high mitotic index. Follow-up revealed local recurrences and metastases, prompting additional surgeries and affecting survival rates. This study reports varying outcomes, with some dogs completing one year without recurrence, while others experienced progressive disease, leading to six oral melanoma-related deaths. The characteristics of melanotic melanoma and amelanotic melanoma are observed in order to study differences between them, the degree of aggressiveness, the mortality rate and the possibility of future therapeutic targets. Although high pigmentation has been correlated with a better outcome, we could not find any significant correlation between survival and achromia. Oral benign melanomas might exist, and this could justify variabilities between stage and survival; however, carefulness is required due to their unpredictable behavior. The findings underscore the complexity of oral melanoma cases and highlight the need for further research on effective management strategies. Full article

Alcohol consumption significantly impacts disease burden and has been linked to various diseases in observational studies. However, comprehensive meta-analyses using Mendelian randomization (MR) to examine drinking patterns are limited. We aimed to evaluate the health risks of alcohol use by integrating findings from MR studies. A thorough search was conducted for MR studies focused on alcohol exposure. We utilized two sets of instrumental variables—alcohol consumption and problematic alcohol use—and summary statistics from the FinnGen consortium R9 release to perform de novo MR analyses. Our meta-analysis encompassed 64 published and 151 de novo MR analyses across 76 distinct primary outcomes. Results show that a genetic predisposition to alcohol consumption, independent of smoking, significantly correlates with a decreased risk of Parkinson’s disease, prostate hyperplasia, and rheumatoid arthritis. It was also associated with an increased risk of chronic pancreatitis, colorectal cancer, and head and neck cancers. Additionally, a genetic predisposition to problematic alcohol use is strongly associated with increased risks of alcoholic liver disease, cirrhosis, both acute and chronic pancreatitis, and pneumonia. Evidence from our MR study supports the notion that alcohol consumption and problematic alcohol use are causally associated with a range of diseases, predominantly by increasing the risk. Full article

Even when fresh, non-alcoholic, and low-alcoholic beers (NABLABs) exhibit significant staling defects due to premature oxidation. In this study, the antioxidant power of eleven fresh commercial NABLABs was assessed by means of three different assays: the oxygen radical absorbance capacity (ORAC), the linoleic acid-induced oxidation (TINH), and the indicator time test (ITT). Only the first two assays, both involving radicalar degradations initiated by AAPH, were found to correlate with each other. NABLABs displayed lower ORAC values than conventional beers (on average, 6127 μmol eq. Trolox/L), except for three samples made with special-colored malts or dry-hopped. Dealcoholization was the step with the greatest impact on the ORAC value (up to a 95% loss) and on flavan-3-ols, sotolon, and polyfunctional thiols, while pasteurization strongly affected color, TBA, and Strecker aldehydes. ORAC assays applied to hop, alternative cereals, and various botanical ingredients indicated that mashing with red sorghum, dry hopping/spicing, and wood maturation could bring the antioxidant power of a NABLAB close to those of conventional beers. With an ORAC value not reached by any other tested botanical ingredient (5234 µmol eq. Trolox/g), African Vernonia amygdalina leaves (traditionally used for Rwandan Ikigage beers) emerged here as the best candidate. Full article

The aim of our study was to investigate the relationship between sleep quality and functional indices, swimming distance and gender in adolescent competitive swimmers. Forty-eight adolescent swimmers (boys, n = 22, 15.7 ± 1.0 years and girls, n = 26, 15.1 ± 0.8 years) were included in our study. They were assessed for handgrip strength, respiratory muscle strength and pulmonary function, answered a Pittsburg Sleep Quality Index questionnaire (PSQI), and recorded their anthropometric and morphological characteristics and training load for the last four weeks. The results showed differences between swimming distance and chest circumference difference, between maximal inhalation and exhalation (Δchest) (p = 0.033), PSQI score (p < 0.001), and sleep quality domains for “cannot breathe comfortably” (p = 0.037) and “have pain” (p = 0.003). Binary logistic regression (chi-square = 37.457, p = 0.001) showed that the variables Δchest (p = 0.038, 95% CI: 1.05–6.07) and PSQI score (p = 0.048, 95% CI: 0.1–1.07) remained independent predictors of the swim distance groups. Girls had a lower percentage of predicted values for the maximal inspiratory pressure (p < 0.001), maximal expiratory pressure (p = 0.027), forced expiratory volume within the first second (p = 0.026), forced vital capacity (p = 0.008) and sleep quality domains for “cough or snore loudly” (p = 0.032) compared to boys. A regression analysis showed that the sleep quality score was explained by the six independent variables: respiratory muscle strength (t = 2.177, β = 0.164, p = 0.035), Δchest (t = −2.353, β = −0.17, p = 0.023), distance (t = −5.962, β = −0.475, p < 0.001), total body water (t = −7.466, β = −0.687, p < 0.001), lean body mass (t = −3.120, β = −0.434, p = 0.003), and handgrip (t = 7.752, β = 1.136, p < 0.001). Our findings demonstrate that sleep quality in adolescent swimmers is a multifactorial result of morphometric characteristics, strength and respiratory function. Full article

This study investigates how missing data samples in continuous blood glucose data affect the prediction of postprandial hypoglycemia, which is crucial for diabetes management. We analyzed the impact of missing samples at different times before meals using two datasets: virtual patient data and real patient data. The study uses six commonly used machine learning models under varying conditions of missing samples, including custom and random patterns reflective of device failures and arbitrary data loss, with different levels of data removal before mealtimes. Additionally, the study explored different interpolation techniques to counter the effects of missing data samples. The research shows that missing samples generally reduce the model performance, but random forest is more robust to missing samples. The study concludes that the adverse effects of missing samples can be mitigated by leveraging complementary and informative non-point features. Consequently, our research highlights the importance of strategically handling missing data, selecting appropriate machine learning models, and considering feature types to enhance the performance of postprandial hypoglycemia predictions, thereby improving diabetes management. Full article

The unprecedented challenges presented by the increase in global population have placed substantial demands on the livestock industry for human nutrition, necessitating heightened animal productivity and leading to an increased demand for natural resources to produce animal feed. Feed producers are leading the charge, consistently refining formulations to adapt to the evolving needs of livestock, driven in part by the cost of over 50% associated with feed production. This paper critically analyses the pressing issues within feed formulation, addressing the requirement for environmentally sustainable practices amidst the challenges of climate change. The exploration extends to how advanced decision support tools can enhance formulation techniques and profitability and contribute to environmental sustainability. Through an in-depth review of current feed formulation technologies, encompassing their applications and limitations, this study aims to enhance the existing knowledge base. Additionally, we examined future trends, highlighting the essential role of connecting technologies to establish a resilient and sustainable system. The emphasis is on the potential of formulation techniques to positively impact the environment and enhance the overall quality and performance of the animals. This paper provides actionable insights to improve animal production by examining feed formulation models and decision support tools. The anticipated outcome is a more informed and sustainable decision-making process, addressing the multifaceted challenges confronted by the livestock industry and making contributions to global efforts in climate change mitigation and environmental stewardship in animal production agriculture. Full article

Bovine ischaemic teat necrosis (ITN) is a disease affecting the skin of the teats of dairy cows with an unknown aetiopathogenesis. Digital dermatitis (DD)-associated treponemes have previously been suggested as a potential aetiological agent in ITN, although the sample size was small. The current study, using established PCR techniques, aimed to examine the association with the presence of DD-associated treponemes in a large number of ITN samples from a wider geographical area, and surveyed the potential of milk as an infection reservoir. From 95 ITN lesions, 35.8% (n = 34) were positive for at least one DD-associated treponeme compared with only 5.6% (n = 1) of 18 non-lesioned teats from cows with ITN lesions on a different teat using a nested PCR approach. All 10 age- and production-matched control cows were negative for DD-associated treponemes via PCR. No DD-associated treponemes could be detected from foremilk of cows with (n = 19) and without (n = 31) a DD lesion on the hind feet. DD-associated treponemes could be detected via PCR after incubation in milk for up to 2 h. Therefore, milk does not appear to be a competent reservoir for transmission of DD-associated treponemes. Moreover, in the current study DD-associated treponemes were only detected in a subset of ITN samples, so it is unlikely these opportunistic skin-associated pathogens are the major or sole agent of ITN. Full article

Increased interest in sustainability and related issues has led to the development of disclosed corporate information on environmental, social, and governance (ESG) issues. Additionally, questions have arisen about whether these disclosures affect the firm’s value. Therefore, we conducted a bibliometric analysis coupled with a systematic literature review (SLR) of the current literature in the Scopus database from 2001 to 2023. We utilized VOS viewer, Site Space, and R Studio tools for this analysis. Our findings indicate that the relationship between ESG disclosures and the firm’s value has different effects and that disclosure impacts through various channels, such as increasing stakeholder trust. Moreover, the keyword analysis results before and after 2015 demonstrate significant advancement in the utilization of a theoretical foundation in the literature. Furthermore, China (Country), “Universidad de Salamanca” (University), “Uyar, Ali and García-Sánchez, Isabel-María” (Authors), and “Sustainability” and “Corporate Social Responsibility and Environmental Management” (Journals) were the most contributing and influential in this field. On the other hand, the results revealed six thematic clusters: society, sustainable development, ESG, organization, innovation, and stakeholders. We found promising research paths and emerging themes through content analysis of these clusters, such as sustainability assurance, green innovation, and sustainable development goals (SDGs). This review concludes by providing a roadmap that includes emerging lines of research that can be explored in depth in future studies to promote better and more comprehensive integration to achieve sustainability and maximize firm value. Full article

As nanoparticle syntheses on a large scale usually yield products with broad size and shape distributions, the properties of nanoparticle-based products need to be tuned after synthesis by narrowing the size and shape distributions or via the removal of undesired fractions. The development of property-selective classification processes requires a universal framework for the quantitative evaluation of multi-dimensional particle fractionation processes. This framework must be applicable to any property and any particle classification process. We extended the well-known one-dimensional methodology commonly used for describing particle size distributions and fractionation processes to the multi-dimensional case to account for the higher complexity of the property distribution and separation functions. In particular, multi-dimensional lognormal distributions are introduced and applied to diameter and length distributions of gold nanorods. The fractionation of nanorods via centrifugation and by orthogonal centrifugal and electric forces is modeled. Moreover, we demonstrate that analytical ultracentrifugation with a multi-wavelength detector (MWL-AUC) is a fast and very accurate method for the measurement of two-dimensional particle size distributions in suspension. The MWL-AUC method is widely applicable to any class of nanoparticles with size-, shape- or composition-dependent optical properties. In addition, we obtained distributions of the lateral diameter and the number of layers of molybdenum disulfide nanosheets via stepwise centrifugation and spectroscopic evaluation of the size fractions. Full article

The Program for International Student Assessment highlights the persistent lack of commitment and motivation among students worldwide in their school activities, which are resulting in decreased proficiency levels in reading, mathematics, and science. The magnitude of this phenomenon, with its clear social implications, suggests that we are facing a concerning quest for immediate answers and results. This research focuses on the impact of the relationships between self-regulated learning processes and the planning of time management that is dedicated to school activities on student performance, specifically in the subjects of the Mother Tongue and Mathematics. The instruments used for analysis included the Inventory of Self-Regulated Learning Processes, the Inventory of Time Management Planning, a personal data sheet, and a school data sheet. The sample in this study consisted of 688 students from primary schools in northern Portugal. The results reveal that self-regulated learning has a positive influence on how students plan time management, both in the short and long term. Additionally, a positive and statistically significant relationship is observed between short-term and long-term time management planning and students’ academic performance. This study provides an in-depth perspective on the dynamics between these elements, shedding light on the crucial nuances that shape students’ academic journeys. Full article

We introduce a novel microscopic image dataset augmented with segmentation and detection labels specifically designed for microplastic analysis in sewage environments. Recognizing the increasing concern over microplastics—particles of synthetic polymers smaller than 5 mm—and their detrimental effects on marine ecosystems and human health, our research focuses on enhancing detection and analytical methodologies through advanced computer vision and deep learning techniques. The dataset comprises high-resolution microscopic images of microplastics collected from sewage, meticulously labeled for both segmentation and detection tasks, aiming to facilitate accurate and efficient identification and quantification of microplastic pollution. In addition to dataset development, we present example deep learning models optimized for segmentation and detection of microplastics within complex sewage samples. The models demonstrate significant potential in automating the analysis of microplastic contamination, offering a scalable solution to environmental monitoring challenges. Furthermore, we ensure the accessibility and reproducibility 12 of our research by making the dataset and model codes publicly available, accompanied by detailed 13 documentation on GitHub and LabelBox. Full article

This study investigates the differential expression of miRNA gene subtypes in tumoral versus benign nevi in individuals with melanoma, aiming to identify clinically significant correlations that could serve as reliable markers for assessing tumor stage and progression. Conducted between 2019 and 2022, this descriptive, quantitative observational research analyzed 90 formalin-fixed paraffin-embedded (FFPE) samples from the Pius Brinzeu County Emergency Clinical Hospital, Timisoara, including 45 samples of advanced-stage melanoma and 45 samples of pigmented nevi. miRNA purification and analysis were performed using the miRNeasy Kit and the Human Cancer PathwayFinder miScript miRNA PCR Array, with statistical analysis (including logistic regression) to determine associations with cancer staging, such as high Breslow index risk, number of mitoses, and vascular invasion. After the analysis and comparison of 180 miRNA gene subtypes, we selected 10 of the most upregulated and 10 most downregulated genes. The results revealed that hsa-miR-133b, hsa-miR-335-5p, hsa-miR-200a-3p, and hsa-miR-885-5p were significantly upregulated in melanoma samples, with fold changes ranging from 1.09 to 1.12. Conversely, hsa-miR-451a and hsa-miR-29b-3p showed notable downregulation in melanoma, with fold changes of 0.90 and 0.92, respectively. Additionally, logistic regression analysis identified hsa-miR-29b-3p (OR = 2.51) and hsa-miR-200a-3p (OR = 2.10) as significantly associated with an increased risk of a high Breslow index, while hsa-miR-127-3p and hsa-miR-451a were associated with a reduced risk. Conclusively, this study underscores the significant alterations in miRNA expression in melanoma compared to benign nevi and highlights the potential of specific miRNAs as biomarkers for melanoma progression. The identification of miRNAs with significant associations to melanoma characteristics suggests their utility in developing non-invasive, cost-effective diagnostic tools and in guiding therapeutic decisions, potentially improving patient outcomes in melanoma management. Full article