The analysis of body motion is a valuable tool in the assessment and diagnosis of gait impairments, particularly those related to neurological disorders. In this study, we propose a novel automated system leveraging artificial intelligence for efficiently analyzing gait impairment from video-recorded images. The proposed methodology encompasses three key aspects. First, we generate a novel one-dimensional representation of each silhouette image, termed a silhouette sinogram, by computing the distance and angle between the centroid and each detected boundary points. This process enables us to effectively utilize relative variations in motion at different angles to detect gait patterns. Second, a one-dimensional convolutional neural network (1D CNN) model is developed and trained by incorporating the consecutive silhouette sinogram signals of silhouette frames to capture spatiotemporal information via assisted knowledge learning. This process allows the network to capture a broader context and temporal dependencies within the gait cycle, enabling a more accurate diagnosis of gait abnormalities. This study conducts training and an evaluation utilizing the publicly accessible INIT GAIT database. Finally, two evaluation schemes are employed: one leveraging individual silhouette frames and the other operating at the subject level, utilizing a majority voting technique. The outcomes of the proposed method showed superior enhancements in gait impairment recognition, with overall F1-scores of 100%, 90.62%, and 77.32% when evaluated based on sinogram signals, and 100%, 100%, and 83.33% when evaluated based on the subject level, for cases involving two, four, and six gait abnormalities, respectively. In conclusion, by comparing the observed locomotor function to a conventional gait pattern often seen in healthy individuals, the recommended approach allows for a quantitative and non-invasive evaluation of locomotion. Full article

A recently discovered haplotype—CYP2C:TG—determines the ultrarapid metabolism of several CYP2C19 substrates. The platelet inhibitor clopidogrel requires CYP2C19-mediated activation: the risk of ischemic events is increased in patients with a poor (PM) or intermediate (IM) CYP2C19 metabolizer phenotype (vs. normal, NM; rapid, RM; or ultrarapid, UM). We investigated whether the CYP2C:TG haplotype affected efficacy/bleeding risk in clopidogrel-treated patients. Adults (n = 283) treated with clopidogrel over 3–6 months were classified by CYP2C19 phenotype based on the CYP2C19*2*17 genotype, and based on the CYP2C19/CYP2C cluster genotype, and regarding carriage of the CYP2:TG haplotype, and were balanced on a number of covariates across the levels of phenotypes/haplotype carriage. Overall, 45 (15.9%) patients experienced ischemic events, and 49 (17.3%) experienced bleedings. By either classification, the incidence of ischemic events was similarly numerically higher in PM/IM patients (21.6%, 21.8%, respectively) than in mutually similar NM, RM, and UM patients (13.2–14.8%), whereas the incidence of bleeding events was numerically lower (13.1% vs. 16.6–20.5%). The incidence of ischemic events was similar in CYP2C:TG carries and non-carries (14.1% vs. 16.1%), whereas the incidence of bleedings appeared mildly lower in the former (14.9% vs. 20.1%). We observed no signal to suggest a major effect of the CYP2C19/CYP2C cluster genotype or CYP2C:TG haplotype on the clinical efficacy/safety of clopidogrel. Full article

The coronavirus disease 2019 (COVID-19) pandemic disrupted public health and economies worldwide. In this paper, we investigate an optimal control problem to simultaneously minimize the epidemic size and control costs associated with intervention strategies based on official data. Considering people with undetected infections, we establish a control system of COVID-19 with time-varying parameters. To estimate these parameters, a parameter identification scheme is adopted and a mixed algorithm is constructed. Moreover, we present an optimal control problem with two objectives that involve the newly increased number of infected individuals and the control costs. A numerical scheme is conducted, simulating the epidemic data pertaining to Shanghai during the period of 2022, caused by the Omicron variant. Coefficient combinations of the objectives are obtained, and the optimal control measures for different infection peaks are indicated. The numerical results suggest that the identification variables obtained by using the constructed mixed algorithm to solve the parameter identification problem are feasible. Optimal control measures for different epidemic peaks can serve as references for decision-makers. Full article

Digital Twin (DT) is widely regarded as a highly promising technology with the potential to revolutionize various industries, making it a key trend in the Industry 4.0 era. In a cost-effective and risk-free setting, digital twins facilitate the interaction and merging of the physical and informational realms. The application of digital twins spans across different sectors, including aerospace, healthcare, smart manufacturing, and smart cities. As electric vehicles have experienced rapid growth, there is a growing demand for the development of innovative technologies. One potential area for digital twins application is within the automotive sector. The powertrain system of electric vehicles (EVs) consists of three parts, power source, power electronic system, and electric motor, which are considered as the core components of electric vehicles. The focus of this paper is to conduct a methodical review regarding the use of digital twins in the powertrain of electric vehicles (EVs). While reviewing the development of digital twin technology, its main application scenarios and its use in electric vehicle powertrains are analysed. Finally, the digital twins currently encounter several challenges that need to be addressed, and so the future development of their application to electric vehicles are summarized. Full article

Background and Objectives: Breastmilk is the safest and most suitable food for an infant, playing the role of their first vaccine and containing all the essential nutrients for the first months of life. The World Health Organisation recommends exclusive breastfeeding for the first 6 months of life and continued breastfeeding while introducing a child to complementary foods until 2 years and beyond. According to Latvian statistics from 2022, only 27.4% of babies were breastfed for 12 months. The aim of this study was to determine the socio-economic factors and factors related to pregnancy, childbirth and postpartum that influence breastfeeding for more than 6 months in Latvia. Materials and Methods: Data were used from the cross-sectional survey “Research on factors and behaviours affecting the sexual and reproductive health of the population of Latvia”, which was conducted in 2023. A study sample was randomised and stratified by gender and five age groups. The analyses in this study are based on a sample of women who had given birth at least once (n = 1407), and the dependent variable was the duration of breastfeeding their last child. Binary logistic regression was conducted to identify the associated factors. Results: The point prevalence of longer duration of breastfeeding for the last child was 47.9% (n = 674). The odds of longer breastfeeding duration were higher among mothers who did not smoke during pregnancy (vs. smokers, aOR 2.1, p < 0.001), of Latvian nationality (vs. Russian, aOR 1.3, p = 0.03), who had two childbirth (vs. one, aOR 1.5, p = 0.003), who had the highest level of education (vs. primary education, aOR 2.0, p = 0.03), started breastfeeding immediately after the birth (vs. later than the first day, aOR 1.7, p = 0.01) or on the first day (vs. later, aOR 1.6, p = 0.01). Conclusions: We documented socio-demographic pregnancy and childbirth factors associated with longer breastfeeding durations. Efforts to promote breastfeeding practices should target mothers from the most vulnerable groups. Full article

Breast Implant-Associated Anaplastic Large Cell Lymphoma (BIA-ALCL) and Breast Implant-Associated Squamous Cell Carcinoma (BIA-SCC) are emerging neoplastic complications related to breast implants. While BIA-ALCL is often linked to macrotextured implants, current evidence does not suggest an implant-type association for BIA-SCC. Chronic inflammation and genetics have been hypothesized as key pathogenetic players, although for both conditions, the exact mechanisms and specific risks related to breast implants are yet to be established. While the genetic alterations in BIA-SCC are still unknown, JAK-STAT pathway activation has been outlined as a dominant signature of BIA-ALCL. Recent genetic investigation has uncovered various molecular players, including MEK-ERK, PI3K/AKT, CDK4-6, and PDL1. The clinical presentation of BIA-ALCL and BIA-SCC overlaps, including most commonly late seroma and breast swelling, warranting ultrasound and cytological examinations, which are the first recommended steps as part of the diagnostic work-up. While the role of mammography is still limited, MRI and CT-PET are recommended according to the clinical presentation and for disease staging. To date, the mainstay of treatment for BIA-ALCL and BIA-SCC is implant removal with en-bloc capsulectomy. Chemotherapy and radiation therapy have also been used for advanced-stage BIA-ALCL and BIA-SCC. In-depth characterization of the tumor genetics is key for the development of novel therapeutic strategies, especially for advanced stage BIA-ALCL and BIA-SCC, which show a more aggressive course and poor prognosis. Full article

I employ autoethnography to undertake a broader scholarly inquiry on intergenerational relationships and transnational care shaped by global migration and aging. Specifically, I reflect on the dynamics of my relationship with my mother, beginning with my departure from my home and spanning a period of 40 years, 8 in China and 34 outside China. In doing so, I contemplate theoretical models of intergenerational solidarity, ambivalence, and role ambiguity. I also challenge cultural assumptions of filial piety. The geographical distance, passage of time, and acculturation process have profoundly influenced my perception of filial piety, which differs markedly from my mother’s. However, this divergence in consensual solidarity—marked by variations in attitudes, beliefs, and values—does not translate into weakened affectual solidarity, characterized by positive sentiments and emotions. Furthermore, aided by advancements in transportation and social media technology, I have been able to extend crucial emotional and some “instrumental” care to my mother, along with financial support if needed, despite limited hands-on care. Nevertheless, I must negotiate my care for my mother and navigate a delicate balance in coordinating my care efforts with those of my non-migrant siblings. Full article

In recent times, there has been a notable surge in the interest in promoting environmentally conscious products, particularly within the building industry where the focus has shifted towards sustainable materials. In this study, as a sustainable building material, ceiling tiles have been fabricated as a composite board containing waste materials, namely waste paper, sawdust, recycled polyethylene terephthalate (PET), and epoxy resin, and characterized comprehensively through physical and mechanical tests, density, thickness swelling (TS), modulus of elasticity (MOE), modulus of rupture (MOR), and flexural strength (FS) for product stability. A total of nine composites were fabricated with different ratios through molding techniques, and the characterization results were compared to determine the optimized stable ratio of composite composition. The composition of 25% waste paper, 15% sawdust, 10% recycled PET, and 50% epoxy resin presented the maximum FS compared to the other composite ratios. Water absorption (WA) and thickness swelling were evaluated after immersion durations of 1–24 h. The findings revealed that as the density increased, the sawdust content within the matrix decreased from 25–35%. Concurrently, an increase in recycled PET content resulted in decreased water absorption and thickness swelling. Significantly, the MOE, MOR, and FS demonstrated optimal values at 864.256 N/mm², 12.786 N/mm², and 4.64 MPa, respectively. These observations represent the excellent qualities of this hybrid composite board, particularly in terms of sustainability, stability, and water absorption capacity. Moreover, its lightweight nature and ability to support ceiling loads further enhance its appeal for construction applications. This study not only advances the discourse on sustainable construction materials but also fosters opportunities for broader acceptance and innovation within the industry. Full article

Traditional neuroimaging methods have identified alterations in brain activity patterns following mild traumatic brain injury (mTBI), particularly during rest, complex tasks, and normal vision. However, studies using graph theory to examine brain network changes in mTBI have produced varied results, influenced by the specific networks and task demands analyzed. In our study, we employed functional MRI to observe 17 mTBI patients and 54 healthy individuals as they viewed a simple, non-narrative underwater film, simulating everyday visual tasks. This approach revealed significant mTBI-related changes in network connectivity, efficiency, and organization. Specifically, the mTBI group exhibited higher overall connectivity and local network specialization, suggesting enhanced information integration without overwhelming the brain’s processing capabilities. Conversely, these patients showed reduced network segregation, indicating a less compartmentalized brain function compared to healthy controls. These patterns were consistent across various visual cortex subnetworks, except in primary visual areas. Our findings highlight the potential of using naturalistic stimuli in graph-based neuroimaging to understand brain network alterations in mTBI and possibly other conditions affecting brain integration. Full article

The lignin derivatives generated during pulping might be responsible for the suboptimal performance of anaerobic reactors during the treatment of pulping wastewater. However, the exact mechanisms by which these derivatives exert influence remain unclear. This study investigated the influence of lignin derivatives, simulated using humic acids (HAs), in anaerobic granular sludge (AnGS). Compared to the enzymes present during floc-bonding and granule-bonding, the HAs impeded the conversion of unhydrolyzed substrates into methane and caused considerable inactivation of free enzymes. Simultaneously, the HAs suppressed agglomeration and weakened the strength of the AnGS. Furthermore, calcium ions helped maintain the integrity of the sludge structure. Therefore, the inhibition of extracellular enzymes using lignin derivatives delays the methanation of unhydrolyzed substrates, resulting in a reduced biomass within AnGS reactors owing to sludge disintegration and biomass loss. This study serves as a reference for investigating the persistent risks originating from lignin derivatives associated with using anaerobic granular-sludge bed reactors to treat pulping wastewater. Full article

Organ-on-chip (OOC) technology has gained importance for biomedical studies and drug development. This technology involves microfluidic devices that mimic the structure and function of specific human organs or tissues. OOCs are a promising alternative to traditional cell-based models and animals, as they provide a more representative experimental model of human physiology. By creating a microenvironment that closely resembles in vivo conditions, OOC platforms enable the study of intricate interactions between different cells as well as a better understanding of the underlying mechanisms pertaining to diseases. OOCs can be integrated with other technologies, such as sensors and imaging systems to monitor real-time responses and gather extensive data on tissue behavior. Despite these advances, OOCs for many organs are in their initial stages of development, with several challenges yet to be overcome. These include improving the complexity and maturity of these cellular models, enhancing their reproducibility, standardization, and scaling them up for high-throughput uses. Nonetheless, OOCs hold great promise in advancing biomedical research, drug discovery, and personalized medicine, benefiting human health and well-being. Here, we review several recent OOCs that attempt to overcome some of these challenges. These OOCs with unique applications can be engineered to model organ systems such as the stomach, cornea, blood vessels, and mouth, allowing for analyses and investigations under more realistic conditions. With this, these models can lead to the discovery of potential therapeutic interventions. In this review, we express the significance of the relationship between mucosal tissues and vasculature in organ-on-chip (OOC) systems. This interconnection mirrors the intricate physiological interactions observed in the human body, making it crucial for achieving accurate and meaningful representations of biological processes within OOC models. Vasculature delivers essential nutrients and oxygen to mucosal tissues, ensuring their proper function and survival. This exchange is critical for maintaining the health and integrity of mucosal barriers. This review will discuss the OOCs used to represent the mucosal architecture and vasculature, and it can encourage us to think of ways in which the integration of both can better mimic the complexities of biological systems and gain deeper insights into various physiological and pathological processes. This will help to facilitate the development of more accurate predictive models, which are invaluable for advancing our understanding of disease mechanisms and developing novel therapeutic interventions. Full article

Zirconium is recognized as one of the main impurities of the rare earth element scandium during purification. It presents significant challenges due to its similar chemical properties, making separating it difficult. This study used trialkyl phosphine oxide (TRPO) as a functional ligand, and the effects of carrier type and acidity on adsorption performance were first investigated. Among these, the novel extraction resin SiO₂-P as a carrier for TRPO demonstrated more prominent separation performance in 0.2 M H₂SO₄ and 5 M HCl solutions. The kinetic and isotherm data were consistent with the pseudo-secondary kinetics and Langmuir model, respectively, and the adsorption process could be regarded as homogeneous monolayer adsorption subject to the dual effects of chemisorption and internal diffusion. In addition, thermodynamic analysis showed that the adsorption process of zirconium under the experimental conditions was a spontaneous endothermic process. Combined with the results of SEM-EDS, FT-IR, and XPS analyses, scandium and zirconium were successfully adsorbed by the resin and uniformly distributed on its surface, and the greater affinity of the P=O groups on the resin for zirconium was the critical factor contributing to the separation of scandium and zirconium. Finally, scandium and zirconium in sulfuric acid and hydrochloric acid media were extracted and separated by column experiments, and the purity of scandium could reach 99.8% and 99.99%, respectively. Full article

Bell peppers (Capsicum annuum) were grown in a greenhouse to evaluate organic fertilizer and foliar seaweed application effects on plant architecture, yield, and fruit quality. Many organic fertilizers contain phytohormones intrinsically. Hydrolyzed and non-hydrolyzed fish fertilizer and cyano-fertilizer treatments were applied in split applications every 7 days over a 135-day growing period. Control plants received no supplemental N. Each fertilizer treatment received applications of one of two different foliar seaweeds or no foliar seaweed in a 4 × 3 factorial design with three replications. Both hydrolyzed and non-hydrolyzed fish fertilizers and cyano-fertilizer increased the number of branches per plant compared to the N-deficient control. The plants receiving cyano-fertilizer or non-hydrolyzed fish fertilizer yielded more than the N-deficient control, and those treatments received 2–3 times the auxin application as the hydrolyzed fish fertilizer. In addition, the leaves from the plants treated with non-hydrolyzed fish fertilizer contained substantially higher levels of abscisic acid, although no abscisic acid was detected in the fertilizers. Both seaweed products decreased the number of fruits that were “bell”-shaped and increased the number of “long”-shaped fruits. Organic fertilizers are complex matrices of nutrients, phytohormones, and other metabolites, making it very challenging to determine the mechanisms behind the observations. Full article

This study investigates codon usage bias within the chloroplast genomes of 18 Taraxacum species, focusing on the base composition and various metrics including GC content, Relative Synonymous Codon Usage (RSCU), Effective Number of Codons (ENc), and GC3s. Our analysis revealed a pronounced preference for A/T-ending codons across Taraxacum species, with GC content across the first, second, and third positions of the codons (GC1, GC2, GC3) and the average GC content consistently below 50%. A detailed examination using the RSCU metric identified 29 commonly preferred A/T-ending codons, indicating a strong codon usage bias towards these endings. Specifically, the codon for leucine (UUA) emerged as highly preferred, while the codon for serine (AGC) was least favored. Through the ENc–GC3s plot analysis, we explored the forces shaping this bias, finding evidence that both mutation pressure and natural selection significantly influence codon preference, with most coding sequences showing weak bias. The PR2 plot analysis further confirmed the role of these factors by demonstrating a higher frequency of T over A and C over G at the third codon position, pointing towards a mutation bias complemented by natural selection. Collectively, our findings highlight a consistent pattern of codon usage bias in the chloroplast genomes of Taraxacum species, influenced by a combination of mutation pressure, natural selection, and possibly other unidentified factors. Full article

Hydrogen gas is attractive as a clean fuel source if it can be produced efficiently without relying on fossil fuels. Biohydrogen production using photosynthetic bacteria may enable environmentally friendly hydrogen production but is currently limited by factors such as low oxygen tolerance. In this study, we isolate a new strain of bacteria that can produce hydrogen under aerial-phase conditions compared with those under liquid-phase conditions in a nitrogen gas or an argon gas atmosphere. Bacterial strains were cultured from scrapings taken from a steel signboard. Investigation of the hydrogen production of the strains under aerial- and liquid-phase conditions and subsequent DNA sequencing led to identification of the bacterium Cereibacter sp. KGU-NF001. Aerial-phase conditions were achieved by filter membranes with the bacterial strains and placing the membranes on medium-soaked cotton wool. The gas atmosphere affected the behavior of the isolated bacterial strains under both aerial- and liquid-phase conditions. Cereibacter sp. KGU-NF001 showed promising oxygen tolerance and was able to maintain hydrogen production of 1.33 mL/mg/d even when the atmosphere contained 12% oxygen. Our findings illustrate that biohydrogen production may be achieved by photosynthetic bacteria under oxygen-containing aerial-phase conditions, indicating a possible pathway to help lower our reliance on fossil fuels. Full article

This research has investigated Italian consumers’ preferences for and purchasing behaviors of strawberries utilizing the Best–Worst Scaling methodology (BWS). This approach enables the key factors that influence strawberry purchasing decisions to be identified and different choice groups to be characterized. To achieve this goal, a survey was conducted on a sample of 496 respondents living in the metropolitan area of Milan (North Italy). The declared preferences of the individuals for 12 strawberry characteristics, divided into intrinsic, extrinsic, and credence attributes, were first measured. A Latent Class Analysis (LCA) was then performed to identify different clusters of consumers according to the individuals’ preferences. Subsequently, the heterogeneity of the clusters was tested, using the Chi-square test, and sociodemographic characteristics and purchasing habits were considered. The results suggest that the most important attribute in the choice of strawberries was appearance, highlighting the importance of preserving it throughout the supply chain, followed by one of the increasingly important aspects of diets, which is health benefits. The attribute considered the least important was the brand. This study demonstrates, from a holistic point of view, that sociodemographic characteristics, food habits, and perceptions of different strawberry attributes influence consumers’ preferences and behaviors. Practical implications suggest a new prospective for communication marketing strategies for producers, creating a better brand identity and highlighting in their marketing all of the aspects that consumers would like to know about the fruits they choose as quality certifications. Full article

Iron compounds can be used in antimicrobial applications by exploiting the toxicity of divalent iron to living organisms due to the Fenton reaction. In this study, the growth inhibitory effects of ferrous sulfate FeSO₄·7H₂O and ferric chloride FeCl₃·6H₂O were observed on Metschnikowia clade and Saccharomyces cerevisiae yeast cells. The relatively high amount of reduced Fe³⁺ to Fe²⁺ in the growth medium determined by Mössbauer spectroscopy may contribute to the antimicrobial activity of ferric chloride. In order to test the reducing ability of sugars in the growth media of yeasts, the reaction of ferric chloride FeCl₃·6H₂O with sugars was investigated. In mixtures of FeCl₃·6H₂O and fructose, approximately two thirds of Fe³⁺ can be reduced to Fe²⁺. When the mixture of FeCl₃·6H₂O and fructose is placed on the surface of aluminum foil, an iron film is formed on the surface of the aluminum due to the reduction by both fructose and aluminum. The relative amount of Fe³⁺ which was reduced to Fe⁰ reached 68%. Full article

Active illumination light becomes strongly reflective interference light after specular reflection. It causes saturation in some areas of the image during target detection, resulting in the inability to recognize detailed target feature information. This greatly limits the application of active illumination detection. Based on the Mueller matrix analysis of the difference in polarization characteristics between the background specular reflected light and the target reflected light, we propose a reflection suppression method based on orthogonal polarization imaging. The method employs a polarization modulation strategy in a bidirectional manner between the light source and the detector. First, the polarization information difference is amplified by active polarized illumination between the background specular reflected light and the target reflected light. Then, the target recovery is achieved by suppressing the background specular reflected light through the polarized orthogonal imaging method. Meanwhile, this method can also be used for moving target detection. The experimental results show that the reflection suppression method of orthogonal polarization imaging can effectively suppress the interference of specular reflection on the target image. Additionally, it can reduce the problems of missed and false detection that occurs in moving target detection and improve the active illumination detection effect. Full article

Three neutral nickel(II) complexes of 3,5–dibromo–salicylaldehyde (3,5–diBr–saloH) were synthesized in the presence or absence of 1,10–phenanthroline (phen) or its derivative 2,9–dimethyl–1,10–phenanthroline (neoc) as co–ligands, namely [Ni(3,5–diBr–salo)₂(neoc)] (complex 1), [Ni(3,5–diBr–salo)₂(phen)] (complex 2) and [Ni(3,5–diBr–salo)₂(H₂O)₂] (complex 3), and were characterized by various techniques. The crystal structure of [Ni(3,5–diBr–salo)₂(neoc)] was determined by single-crystal X-ray crystallography. According to employed studying techniques, the complexes interact tightly with calf-thymus DNA by an intercalative fashion. Furthermore, compounds 1–3 bind tightly and reversibly to human and bovine serum albumin. Full article

This study represents a pioneering investigation and comparative analysis of lipid extracts from four different colors of peach palm (Bactris gasipaes Kunt) fruits—red, yellow, green, and white—by employing a green method based on ethanolic ultrasound-assisted extraction. This study examined the extraction yield, physico-chemical-quality attributes, chromatographic profiles (GC), color measurements, total carotenoid content, differential thermogravimetry (TG/DTA), and infrared spectroscopy (FTIR). The obtained lipid extracts displayed a high quality, considering the physico-chemical parameters of the Codex Alimentarius, and a fatty acids profile characterized by unsaturated fatty acids, notably omegas (ω-3, ω-6, and ω-9). The indices of atherogenicity (A.I.), thrombogenicity (I.T.), and hypocholesterolemic and hypercholesterolemic ratios revealed superior outcomes for the red peach palm lipid extract (approximately 0.35, 0.52, and 2.75, respectively), along with higher levels of β-carotene (748.36 µg of β-carotene per 100 g⁻¹ of lipid extract) compared to the yellow, green, and white counterparts. Consequently, this research successfully demonstrates the efficacy of using a green extraction method in preserving the lipid’s quality, which can display cardiovascular functionality and thermal stability. These findings underscore the considerable potential of peach palm lipid extract as a valuable raw material for diverse industrial applications across various sectors. The results support its utilization in the production of functional food products and nutraceuticals due to its favorable fatty acid composition, potent antioxidant properties exhibited by its high β-carotene content, and notable cardiovascular functionality indices. Full article

Extending the shelf life of exported beef could increase international demand and producer profits. The objective was to evaluate the effects of topically applying combinations of acerola cherry powder and rosemary extract on the shelf life of frozen–thawed bone-in beef short rib and chuck roll steaks. Chuck rolls (IMPS 116A; N = 9) and bone-in short ribs (IMPS 123A; N = 18) were aged (7 d; 0 °C), frozen (30 d; −20 °C), and thawed (60–72 h; 0 °C). Steaks measuring 1.02 cm thick were treated and subjected to a 4 d retail display. Steaks were left untreated (control) or sprayed topically with acerola cherry powder (0.05%; A), rosemary extract (0.10%; R), or a combination (M1 = 0.05% A + 0.1% R; M2 = 0.1% A + 0.1% R; M3 = 0.05% A + 0.2% R; M4 = 0.1% A + 0.2% R). Chuck roll M2- and M4-treated steaks were redder than the control steaks on days 3 and 4 (p = 0.008), and antioxidant-treated steaks had less lipid oxidation on day 4 than the control steaks (p = 0.021). Bone marrow samples treated with R, M3, and M4 were redder than the control on days 1–3 (p = 0.014), and bone marrow treated with M3 was subjectively redder compared to the control on days 0 and 1 (p = 0.033). Topical antioxidants improve the redness and delay the oxidation of frozen–thawed beef. Full article

Reactive oxygen species (ROS) play an important and controversial role in carcinogenesis. Microsomal redox chains containing NADH- and NADPH-dependent oxidoreductases are among the main sites of intracellular ROS synthesis, but their role in the oxidative balance has not been fully studied. Here, we studied the activity of cytochrome b5 reductase (CYB5R) and cytochrome P450 reductase (CYPOR) in ovarian cancer tissues and cells isolated from peritoneal fluid, along with the antioxidant capacity of peritoneal fluid. We used the developed a chemiluminescence assay based on stimulation with NADH and NADPH, which reflects the activity of CYB5R and CYPOR, respectively. The activity of CYB5R and CYPOR was significantly higher in moderately and poorly differentiated ovarian adenocarcinomas compared with well-differentiated adenocarcinomas and cystadenomas. For the chemotherapy-resistant tumors, the activity of tissue CYB5R and CYPOR was lower compared to the non-resistant tumors. In the peritoneal fluid, the antioxidant capacity significantly increased in this series, benign tumors < well-differentiated < moderately and poorly differentiated adenocarcinomas, so the antioxidant excess was observed for moderately and poorly differentiated adenocarcinomas. The antioxidant capacity of peritoneal fluid and the activity of CYB5R and CYPOR of cells isolated from peritoneal fluid were characterized by a direct moderate correlation for moderately and poorly differentiated adenocarcinomas. These results indicate the significant role of NAD(P)H oxidoreductases and the antioxidant potential of peritoneal fluid in cancer biochemistry. The parameters studied are useful for diagnostics and prognostics. The developed assay can be used to analyze CYB5R and CYPOR activity in other tissues and cells. Full article