The 2023 MDPI Annual Report has
been released!
 
11 pages, 2913 KiB  
Communication
Overexpression of Larch SCL6 Inhibits Transitions from Vegetative Meristem to Inflorescence and Flower Meristem in Arabidopsis thaliana (L.) Heynh.
by Jun-Xia Xing, Qiao-Lu Zang, Zha-Long Ye, Li-Wang Qi, Ling Yang and Wan-Feng Li
Plants 2024, 13(9), 1232; https://doi.org/10.3390/plants13091232 (registering DOI) - 29 Apr 2024
Abstract
SCARECROW-LIKE6 (SCL6) plays a role in the formation and maintenance of the meristem. In Larix kaempferi (Lamb.) Carr., an important afforestation tree species in China, SCL6 (LaSCL6) has two alternative splicing variants—LaSCL6-var1 and LaSCL6-var2—which are regulated by [...] Read more.
SCARECROW-LIKE6 (SCL6) plays a role in the formation and maintenance of the meristem. In Larix kaempferi (Lamb.) Carr., an important afforestation tree species in China, SCL6 (LaSCL6) has two alternative splicing variants—LaSCL6-var1 and LaSCL6-var2—which are regulated by microRNA171. However, their roles are still unclear. In this study, LaSCL6-var1 and LaSCL6-var2 were transformed into the Arabidopsis thaliana (L.) Heynh. genome, and the phenotypic characteristics of transgenic A. thaliana, including the germination percentage, root length, bolting time, flower and silique formation times, inflorescence axis length, and branch and silique numbers, were analyzed to reveal their functions. It was found that LaSCL6-var1 and LaSCL6-var2 overexpression shortened the root length by 41% and 31%, respectively, and increased the inflorescence axis length. Compared with the wild type, the bolting time in transgenic plants was delayed by approximately 2–3 days, the first flower and silique formation times were delayed by approximately 3–4 days, and the last flower and silique formation times were delayed by about 5 days. Overall, the life cycle in transgenic plants was prolonged by approximately 5 days. These results show that LaSCL6 overexpression inhibited the transitions from the vegetative meristem to inflorescence meristem and from the flower meristem to meristem arrest in A. thaliana, revealing the roles of LaSCL6-var1 and LaSCL6-var2 in the fate transition and maintenance of the meristem. Full article
(This article belongs to the Special Issue Molecular Biology and Bioinformatics of Forest Trees)
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15 pages, 8219 KiB  
Article
An Experiment-Based Variable Compensation Method to Improve the Geometric Accuracy of Sub-Mm Features Fabricated by Stereolithography (SLA)
by Francesco Modica, Vito Basile and Irene Fassi
J. Manuf. Mater. Process. 2024, 8(3), 90; https://doi.org/10.3390/jmmp8030090 (registering DOI) - 29 Apr 2024
Abstract
In this paper, we present an experimental procedure to enhance the dimensional accuracy of fabrication via stereolithography (SLA) of features at the sub-mm scale. Deviations in sub-mm hemispherical cavity diameters were detected and measured on customized samples by confocal microscopy. The characterization and [...] Read more.
In this paper, we present an experimental procedure to enhance the dimensional accuracy of fabrication via stereolithography (SLA) of features at the sub-mm scale. Deviations in sub-mm hemispherical cavity diameters were detected and measured on customized samples by confocal microscopy. The characterization and experimental observations of samples allowed the identification of inaccuracy sources, mainly due to the laser beam scanning strategy and the incomplete removal of uncured liquid resin in post-processing (i.e., IPA washing). As a technology baseline, the measured dimensional errors on cavity diameters were up to −46%. A compensation method was defined and implemented, resulting in relevant improvements in dimensional accuracy. However, measurements on sub-mm cavities having different sizes revealed that a constant compensation parameter (i.e., C = 85, 96, 120 μm) is not fully effective at the sub-mm scale, where average errors remain at −24%, −18.8%, and −16% for compensations equal to 85, 96 and 120 μm, respectively. A further experimental campaign allowed the identification of an effective nonlinear compensation law where the compensation parameter depends on the sub-mm feature size C = f(D). Results show a sharp improvement in dimensional accuracy on sub-mm cavity fabrication, with errors consistently below +8.2%. The proposed method can be extended for the fabrication of any sub-mm features without restrictions on the specific technology implementation. Full article
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14 pages, 2338 KiB  
Article
Development and Validation of a Novel Zero-Dimensional Heat Rejection Model for High-Efficiency Engines
by Francesca Furia, Vittorio Ravaglioli, Alberto Cerofolini and Carlo Bussi
Energies 2024, 17(9), 2116; https://doi.org/10.3390/en17092116 (registering DOI) - 29 Apr 2024
Abstract
In recent years, the trend towards the performance maximization of modern internal combustion engines has led to the creation of accurate simulation models to optimize the engine design and operating conditions. Temperature management is crucial to achieve the performance goals of an internal [...] Read more.
In recent years, the trend towards the performance maximization of modern internal combustion engines has led to the creation of accurate simulation models to optimize the engine design and operating conditions. Temperature management is crucial to achieve the performance goals of an internal combustion engine without affecting the component’s reliability. Formula 1 mandates that only a limited number of experimental tests can be performed, which leads to the necessity of simulators capable of substituting empirical tests. Furthermore, the requirement of adapting the vehicle setup before each race weekend to maximize the performance on each circuit layout necessitates short computational time. To address this, the development of a zero-dimensional model of the thermal flows within an engine is presented in this paper. This model allows to precisely compute the dynamic variations of all the heat flows inside the combustion engine, excluding only the radiative ones and the engine components’ temperatures. The new simulation approach has been developed and validated on a Formula 1 engine and shown to be precise and fast. The results demonstrate the value of the proposed model with an average engine fluid temperature error of less than 1 °C for a computational cost comparable with on-board applications. Full article
(This article belongs to the Special Issue Internal Combustion Engine: Research and Application—2nd Edition)
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11 pages, 1290 KiB  
Communication
Mueller Matrix Polarizing Power
by José J. Gil
Photonics 2024, 11(5), 411; https://doi.org/10.3390/photonics11050411 (registering DOI) - 29 Apr 2024
Abstract
The transformation of the states of polarization of electromagnetic waves through their interaction with polarimetrically linear media can be represented by the associated Mueller matrices. A global measure of the ability of a linear medium to modify the states of polarization of incident [...] Read more.
The transformation of the states of polarization of electromagnetic waves through their interaction with polarimetrically linear media can be represented by the associated Mueller matrices. A global measure of the ability of a linear medium to modify the states of polarization of incident waves, due to any combination of enpolarizing, depolarizing and retarding properties, is introduced as the distance from the Mueller matrix to the identity matrix. This new descriptor, called the polarizing power, is applicable to any Mueller matrix and can be expressed as a function of the degree of polarimetric purity and the trace of the Mueller matrix. The graphical representation of the feasible values of the polarizing power provides a general view of its main peculiarities and features. The values of the polarizing power for several typical devices are analyzed. Full article
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16 pages, 26284 KiB  
Article
Leads with the Cut Proximal Ends Migrated into the Heart and Vasculature: A Rare Phenomenon among 3847 Lead Extraction Procedures
by Andrzej Kutarski, Wojciech Jacheć, Radosław Pietura, Paweł Stefańczyk, Jarosław Kosior, Marek Czajkowski, Sebastian Sawonik, Łukasz Tułecki and Dorota Nowosielecka
J. Clin. Med. 2024, 13(9), 2602; https://doi.org/10.3390/jcm13092602 (registering DOI) - 29 Apr 2024
Abstract
Background: The study aimed to describe the phenomenon of leads migrated (MPLE) into the cardiovascular system (CVS). Methods: Retrospective analysis of 3847 transvenous lead extractions (TLE). Results: Over a 17-year period, 72 (1.87%) MPLEs (median dwell time 137.5 months) were [...] Read more.
Background: The study aimed to describe the phenomenon of leads migrated (MPLE) into the cardiovascular system (CVS). Methods: Retrospective analysis of 3847 transvenous lead extractions (TLE). Results: Over a 17-year period, 72 (1.87%) MPLEs (median dwell time 137.5 months) were extracted, which included mainly ventricular leads (56.94%). Overall, 68.06% of MPLEs had their cut proximal ends in the venous system. Most of them were pacing (95.83%) and passive fixation (98.61%) leads. Independent risk factors for MPLE included abandoned leads (OR = 8.473; p < 0.001) and leads located on both sides of the chest (2.981; p = 0.045). The higher NYHA class lowered the probability of MPLE (OR = 0.380; p < 0.001). Procedure complexity was higher in the MPLE group (procedure duration, unexpected procedure difficulties, use of additional (advanced) tools and alternative venous approach). There were no more major complications in the MPLE group, but the rate of procedural success was lower due to more frequent retention of non-removable lead fragments. Extraction of MPLEs did not influence long-term survival. Conclusions: 1. Extraction of leads with MPLE is rare among other TLE procedures (1.9%), 2. risk factors include abandoned leads and presence of leads on both sides of the chest but a higher NYHA class lowers the probability of MPLE, 3. complexity of MPLE extraction is higher regarding procedure duration, unexpected procedure difficulties, use of advanced tools and techniques but rates of major complications are comparable, and 4. extraction of MPLEs did not influence long-term survival. Full article
(This article belongs to the Special Issue Transvenous Lead Extraction - Progress in Lead Management)
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14 pages, 3748 KiB  
Article
Modulation Steering Motion by Quantitative Electrical Stimulation in Pigeon Robots
by Mingxuan Bi, Huimin Zhang, Yaohong Ma, Hao Wang, Wenbo Wang, Yuan Shi, Wenlong Sheng, Qiushun Li, Guangheng Gao and Lei Cai
Micromachines 2024, 15(5), 595; https://doi.org/10.3390/mi15050595 (registering DOI) - 29 Apr 2024
Abstract
The pigeon robot has attracted significant attention in the field of animal robotics thanks to its outstanding mobility and adaptive capability in complex environments. However, research on pigeon robots is currently facing bottlenecks, and achieving fine control over the motion behavior of pigeon [...] Read more.
The pigeon robot has attracted significant attention in the field of animal robotics thanks to its outstanding mobility and adaptive capability in complex environments. However, research on pigeon robots is currently facing bottlenecks, and achieving fine control over the motion behavior of pigeon robots through brain–machine interfaces remains challenging. Here, we systematically quantify the relationship between electrical stimulation and stimulus-induced motion behaviors, and provide an analytical method to demonstrate the effectiveness of pigeon robots based on electrical stimulation. In this study, we investigated the influence of gradient voltage intensity (1.2–3.0 V) on the indoor steering motion control of pigeon robots. Additionally, we discussed the response time of electrical stimulation and the effective period of the brain–machine interface. The results indicate that pigeon robots typically exhibit noticeable behavioral responses at a 2.0 V voltage stimulus. Increasing the stimulation intensity significantly controls the steering angle and turning radius (p < 0.05), enabling precise control of pigeon robot steering motion through stimulation intensity regulation. When the threshold voltage is reached, the average response time of a pigeon robot to the electrical stimulation is 220 ms. This study quantifies the role of each stimulation parameter in controlling pigeon robot steering behavior, providing valuable reference information for the precise steering control of pigeon robots. Based on these findings, we offer a solution for achieving precise control of pigeon robot steering motion and contribute to solving the problem of encoding complex trajectory motion in pigeon robots. Full article
(This article belongs to the Special Issue Intelligent Bioelectronics and Neural Interfaces)
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12 pages, 4583 KiB  
Article
Mendelian Randomization Reveals: Triglycerides and Sensorineural Hearing Loss
by Shun Ding, Yixuan Liu, Tingting Duan, Peng Fang, Qiling Tong, Huawei Li and Huiqian Yu
Bioengineering 2024, 11(5), 438; https://doi.org/10.3390/bioengineering11050438 (registering DOI) - 29 Apr 2024
Abstract
Background: Sensorineural hearing loss (SNHL) is a multifactorial disorder with potential links to various physiological systems, including the cardiovascular system via blood lipid levels such as triglycerides (TG). This study investigates the causal relationship between TG levels and SNHL using Mendelian randomization (MR), [...] Read more.
Background: Sensorineural hearing loss (SNHL) is a multifactorial disorder with potential links to various physiological systems, including the cardiovascular system via blood lipid levels such as triglycerides (TG). This study investigates the causal relationship between TG levels and SNHL using Mendelian randomization (MR), which offers a method to reduce confounding and reverse causality by using genetic variants as instrumental variables. Methods: Utilizing publicly available genome-wide association study (GWAS) data, we performed a two-sample MR analysis. The initial analysis unveiled a causal relationship between TG (GWAS ID: ebi-a-GCST90018975) and SNHL (GWAS ID: finn b-H8_HL_SEN-NAS). Subsequent analysis validated this through MR with a larger sample size for TG (GWAS ID: ieu-b-111) and SNHL. To conduct the MR analysis, we utilized several methods including inverse-variance weighted (IVW), MR Egger, weighted median, and weighted mode. We also employed Cochrane’s Q test to identify any heterogeneity in the MR results. To detect horizontal pleiotropy, we conducted the MR-Egger intercept test and MR pleiotropy residual sum and outliers (MR-PRESSO) test. We performed a leave-one-out analysis to assess the sensitivity of this association. Finally, a meta-analysis of the MR results was undertaken. Results: Our study found a significant positive correlation between TG and SNHL, with OR values of 1.14 (95% CI: 1.07–1.23, p < 0.001) in the IVW analysis and 1.09 (95% CI: 1.03–1.16, p < 0.006) in the replicate analysis. We also found no evidence of horizontal pleiotropy or heterogeneity between the genetic variants (p > 0.05), and a leave-one-out test confirmed the stability and robustness of this association. The meta-analysis combining the initial and replicate analyses showed a significant causal effect with OR values of 1.11 (95% CI: 1.06–1.16, p = 0.01). Conclusion: These findings indicate TG as a risk factor for SNHL, suggesting potential pathways for prevention and intervention in populations at risk. This conclusion underscores the importance of managing TG levels as a strategy to mitigate the risk of developing SNHL. Full article
(This article belongs to the Section Biomedical Engineering and Biomaterials)
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13 pages, 2987 KiB  
Article
Isolation, Characterization, and Biopreservation of Lactobacillus brevis DN-1 to Inhibit Mold and Remove Aflatoxin B1 in Peanut and Sunflower Cakes
by Xiaoni Wang, Siyuan Wang, Junzhao Xu, Baiyila Wu, Zongfu Hu and Huaxin Niu
Agriculture 2024, 14(5), 698; https://doi.org/10.3390/agriculture14050698 (registering DOI) - 29 Apr 2024
Abstract
Aflatoxin B1 (AFB1) is the most toxic mycotoxin and is widespread in moldy feed. The use of biological removal methods to reduce AFB1 has become a research hotspot. This study aimed to isolate lactic acid bacteria (LAB) capable of removing AFB1 from moldy [...] Read more.
Aflatoxin B1 (AFB1) is the most toxic mycotoxin and is widespread in moldy feed. The use of biological removal methods to reduce AFB1 has become a research hotspot. This study aimed to isolate lactic acid bacteria (LAB) capable of removing AFB1 from moldy feeds and assessed the removal capacity under various environmental conditions. A strain named Lactobacillus brevis DN-1 was isolated from feed samples and showed 71.38% AFB1 percent removal. Furthermore, DN-1 showed good antifungal activity against Aspergillus flavus BNCC336156 and Aspergillus parasiticus BNCC335939. The optimum growth temperature and pH of DN-1 were 37 °C and 6.0, respectively, and DN-1 grew well in the concentration range of 0–20 µg/L AFB1. Under a temperature of 20–40 °C, pH of 3.0–9.0, and anaerobic conditions, the percent removal of AFB1 was more than 60%. An analysis of the different components of DN-1 showed that cell wall adsorption was the main removal method and suggested the pathway for AFB1 removal by LAB. In addition, strain DN-1 was used as a biological preservative in artificially contaminated peanut and sunflower cakes, which significantly inhibited the growth of mold and production of AFB1. In brief, this study highlights the potential use of DN-1 as a preventive agent against aflatoxicosis via strong removal capability in the application of fermented feed or food. Full article
(This article belongs to the Section Agricultural Product Quality and Safety)
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20 pages, 10661 KiB  
Article
Examination of Cardiac Activity with ECG Monitoring Using Heart Rate Variability Methods
by Galya Georgieva-Tsaneva, Evgeniya Gospodinova and Krasimir Cheshmedzhiev
Diagnostics 2024, 14(9), 926; https://doi.org/10.3390/diagnostics14090926 (registering DOI) - 29 Apr 2024
Abstract
The paper presents a system for analyzing cardiac activity with the possibility of continuous and remote monitoring. The created sensor mobile device monitors heart activity by means of the convenient and imperceptible registration of cardiac signals. At the same time, the behavior of [...] Read more.
The paper presents a system for analyzing cardiac activity with the possibility of continuous and remote monitoring. The created sensor mobile device monitors heart activity by means of the convenient and imperceptible registration of cardiac signals. At the same time, the behavior of the human body is also monitored through the accelerometer and gyroscope built into the device, thanks to which it is possible to signal in the event of loss of consciousness or fall (in patients with syncope). Conducting real-time cardio monitoring and the analysis of recordings using various mathematical methods (linear, non-linear, and graphical) enables the research, accurate diagnosis, timely assistance, and correct treatment of cardiovascular diseases. The paper examines the recordings of patients diagnosed with arrhythmia and syncope recorded by electrocardiography (ECG) sensors in real conditions. The obtained results are subjected to statistical analysis to determine the accuracy and significance of the obtained results. The studies show significant deviations in the patients with arrhythmia and syncope regarding the obtained values of the studied parameters of heart rate variability (HRV) from the accepted normal values (for example, the root mean square of successive differences between normal heartbeats (RMSSD) in healthy individuals is 24.02 ms, while, in patients with arrhythmia (6.09 ms) and syncope (5.21 ms), it is much lower). The obtained quantitative and graphic results identify some possible abnormalities and demonstrate disorders regarding the activity of the autonomic nervous system, which is directly related to the work of the heart. Full article
(This article belongs to the Section Pathology and Molecular Diagnostics)
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17 pages, 3520 KiB  
Article
Hepatocyte-Specific Fads1 Overexpression Attenuates Western Diet-Induced Metabolic Phenotypes in a Rat Model
by Dushan T. Ghooray, Manman Xu, Hongxue Shi, Craig J. McClain and Ming Song
Int. J. Mol. Sci. 2024, 25(9), 4836; https://doi.org/10.3390/ijms25094836 (registering DOI) - 29 Apr 2024
Abstract
Fatty acid desaturase 1 (FADS1) is a rate-limiting enzyme in long-chain polyunsaturated fatty acid (LCPUFA) synthesis. Reduced activity of FADS1 was observed in metabolic dysfunction-associated steatotic liver disease (MASLD). The aim of this study was to determine whether adeno-associated virus serotype 8 (AAV8) [...] Read more.
Fatty acid desaturase 1 (FADS1) is a rate-limiting enzyme in long-chain polyunsaturated fatty acid (LCPUFA) synthesis. Reduced activity of FADS1 was observed in metabolic dysfunction-associated steatotic liver disease (MASLD). The aim of this study was to determine whether adeno-associated virus serotype 8 (AAV8) mediated hepatocyte-specific overexpression of Fads1 (AAV8-Fads1) attenuates western diet-induced metabolic phenotypes in a rat model. Male weanling Sprague-Dawley rats were fed with a chow diet, or low-fat high-fructose (LFHFr) or high-fat high-fructose diet (HFHFr) ad libitum for 8 weeks. Metabolic phenotypes were evaluated at the endpoint. AAV8-Fads1 injection restored hepatic FADS1 protein levels in both LFHFr and HFHFr-fed rats. While AAV8-Fads1 injection led to improved glucose tolerance and insulin signaling in LFHFr-fed rats, it significantly reduced plasma triglyceride (by ~50%) and hepatic cholesterol levels (by ~25%) in HFHFr-fed rats. Hepatic lipidomics analysis showed that FADS1 activity was rescued by AAV8-FADS1 in HFHFr-fed rats, as shown by the restored arachidonic acid (AA)/dihomo-γ-linolenic acid (DGLA) ratio, and that was associated with reduced monounsaturated fatty acid (MUFA). Our data suggest that the beneficial role of AAV8-Fads1 is likely mediated by the inhibition of fatty acid re-esterification. FADS1 is a promising therapeutic target for MASLD in a diet-dependent manner. Full article
(This article belongs to the Special Issue Nutrition, Obesity and Metabolic Syndrome)
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16 pages, 704 KiB  
Article
Ethnic Background of the Two Feeding Stories in Mark’s Gospel
by Paula Andrea García Arenas
Religions 2024, 15(5), 553; https://doi.org/10.3390/rel15050553 (registering DOI) - 29 Apr 2024
Abstract
The analysis delves into the conflict inherent within the thematic discourse surrounding the two tables as portrayed in Mark’s Gospel, with particular emphasis on the section concerning the multiplication of loaves of bread (Mk 6–8). Noteworthy is the conflict arising from the juxtaposition [...] Read more.
The analysis delves into the conflict inherent within the thematic discourse surrounding the two tables as portrayed in Mark’s Gospel, with particular emphasis on the section concerning the multiplication of loaves of bread (Mk 6–8). Noteworthy is the conflict arising from the juxtaposition of Jewish and pagan individuals at a shared table. This theological tension finds resonance in the narratives presented by Paul in Galatians and Romans, albeit Galatians 2:9 intimates a seemingly facile resolution, a departure from the intricate portrayal in Mark’s Gospel. Mark’s narrative accentuates two salient dimensions: firstly, the ethnic substrate of the conflict, and secondly, its contextual specificity within the historical milieu of Syria after the Jewish war. The ethnic genesis of this conflict, as delineated in the accounts of Flavius Josephus, furnishes a background essential for comprehending the dual incidents of bread multiplication: the initial instance catering exclusively to Jews and the subsequent occurrence inclusive of both Jews and other disparate ethnic groups “from afar” (Mk 8:3). The spatial symbolism in the section pertaining to the multiplication of loaves may symbolically represent the heterogeneous composition of the recipients, thereby exacerbating the challenges inherent in reconciling conflicts rooted in ethnic diversity. Full article
(This article belongs to the Special Issue The Bible within Ancient and Modern Cultures)
10 pages, 431 KiB  
Article
The Impact of Financial Pressure on the Association between Employment and Depressive Symptoms for Community-Dwelling Older Adults
by Hiroko Costantini, Takazumi Ono, Nanami Shogaki, Tomoki Tanaka, Weida Lyu, Bo-Kyung Son, Yasuyo Yoshizawa and Katsuya Iijima
J. Ageing Longev. 2024, 4(2), 41-50; https://doi.org/10.3390/jal4020004 (registering DOI) - 29 Apr 2024
Abstract
Addressing how employment impacts older adults, including their psychological well-being, we contribute to the study of this topic by examining the association between paid work in community-dwelling older adults and their depressive mood, while considering the extent of their financial pressure. Methods: The [...] Read more.
Addressing how employment impacts older adults, including their psychological well-being, we contribute to the study of this topic by examining the association between paid work in community-dwelling older adults and their depressive mood, while considering the extent of their financial pressure. Methods: The data are from the Kashiwa longitudinal cohort study, with a 2014 baseline and a 2016 follow-up. Of the 1308 participants in the 2014 survey, 781 people were included. We conducted binary logistic regression analyses stratified by economic status with regard to the extent of the financial pressure experienced. The independent variable of interest was paid work in 2014, and the dependent variable was the extent of depressive mood (a score of 5 or more on the Geriatric Depression Scale 15) in 2016. Results: Paid work was associated with lower odds of depressive mood among those under financial pressure (odds ratio [OR] 0.46, 95% confidence interval [95%CI] 0.26, 0.81), whereas for those financially stable, we did not find an association between paid work and odds of depressive mood (OR 0.99, [95%CI] 0.26, 2.63). Discussion: We evidence that for older adults under financial pressure, employment supports the maintenance of their psychological well-being. We contribute to the literature by understanding when employment is beneficial for older adults, which is important in developing appropriate older adult employment social policies. Full article
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18 pages, 1992 KiB  
Article
Resilience of Terraced Landscapes to Human and Natural Impacts: A GIS-Based Reconstruction of Land Use Evolution in a Mediterranean Mountain Valley
by Titouan Le Vot, Marianne Cohen, Maciej Nowak, Paul Passy and Franck Sumera
Land 2024, 13(5), 592; https://doi.org/10.3390/land13050592 (registering DOI) - 29 Apr 2024
Abstract
Terraced historical landscapes have multiple functions in mountain land, limiting erosion, enabling agricultural production and constituting cultural heritage. Currently, they are largely abandoned in Mediterranean regions and facing the ongoing impacts of climate change. Our aim is to reconstruct the evolution of land [...] Read more.
Terraced historical landscapes have multiple functions in mountain land, limiting erosion, enabling agricultural production and constituting cultural heritage. Currently, they are largely abandoned in Mediterranean regions and facing the ongoing impacts of climate change. Our aim is to reconstruct the evolution of land use on the terraces in order to test the hypothesis of the resilience of these landscapes and their age in recent history (17th–21st century). To achieve this, we used various current and archive spatial datasets and GIS knowledge to detect and map terraces and the changes in land use. We tested this hypothesis in a territory impacted by a recent extreme event, facing the challenge of its reconstruction. Our main outcome showed that the optimal use of the terraces corresponded to the demographic optimum of the mid-19th century, and they were gradually abandoned after the Second World War, with significant differences between Mediterranean and mountain lands. Despite this evolution, the terraces persisted and withstood an extreme event, validating our resilience hypothesis and opening avenues for the revitalization of this territory based on this heritage. These findings are drawing perspectives for the future of terraced landscapes in Mediterranean mountains in the context of climate change. Full article
(This article belongs to the Special Issue Resilience in Historical Landscapes)
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12 pages, 773 KiB  
Article
The Protective Effect of the Crosstalk between Zinc Hair Concentration and Lymphocyte Count—Preliminary Report
by Tomasz Urbanowicz, Anetta Hanć, Jolanta Tomczak, Michał Michalak, Anna Olasińska-Wiśniewska, Patrycja Rzesoś, Mateusz Szot, Krzysztof J. Filipiak, Beata Krasińska, Zbigniew Krasiński, Andrzej Tykarski and Marek Jemielity
Life 2024, 14(5), 571; https://doi.org/10.3390/life14050571 (registering DOI) - 29 Apr 2024
Abstract
Background: An imbalance between pro- and anti-inflammatory mechanisms is indicated in the pathophysiology of atherosclerotic plaque. The coronary artery and carotid disease, despite sharing similar risk factors, are developed separately. The aim of this study was to analyze possible mechanisms between trace element [...] Read more.
Background: An imbalance between pro- and anti-inflammatory mechanisms is indicated in the pathophysiology of atherosclerotic plaque. The coronary artery and carotid disease, despite sharing similar risk factors, are developed separately. The aim of this study was to analyze possible mechanisms between trace element hair–scalp concentrations and whole blood counts that favor atherosclerotic plaque progression in certain locations. Methods: There were 65 (36 (55%) males and 29 (45%) females) patients with a median age of 68 (61–73) years enrolled in a prospective, preliminary, multicenter analysis. The study group was composed of 13 patients with stable coronary artery disease (CAD group) referred for surgical revascularization due to multivessel coronary disease, 34 patients with carotid artery disease (carotid group) admitted for vascular procedure, and 18 patients in a control group (control group). Results: There was a significant difference between the CAD and carotid groups regarding lymphocyte (p = 0.004) counts. The biochemical comparison between the coronary and carotid groups revealed significant differences regarding chromium (Cr) (p = 0.002), copper (Cu) (p < 0.001), and zinc (Zn) (p < 0.001) concentrations. Spearman Rank Order Correlations between lymphocyte counts and trace elements in the analyzed groups were performed, revealing a strong correlation with zinc (R = 0.733, p < 0.001) in the control group (non-CAD, non-carotid). Conclusion: Significant differences in hair–scalp concentrations related to atherosclerosis location were observed in our analysis. The interplay between zinc concentration and lymphocyte count may play a pivotal role in cardiovascular disease development. Full article
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15 pages, 623 KiB  
Article
Intelligent Dynamic Power Control with Cell Range Expansion for Small-Cells in 5G HetNets
by Ilhak Ban and Se-Jin Kim
Appl. Sci. 2024, 14(9), 3789; https://doi.org/10.3390/app14093789 (registering DOI) - 29 Apr 2024
Abstract
In 5G heterogeneous networks (HetNets), small-cell base stations (SBSs) are deployed in the coverage of macro base stations (MBSs) to improve the system performance. However, some macro user equipments (MUEs) have strong interference from neighboring SBSs and thus the performance of MBSs decreases. [...] Read more.
In 5G heterogeneous networks (HetNets), small-cell base stations (SBSs) are deployed in the coverage of macro base stations (MBSs) to improve the system performance. However, some macro user equipments (MUEs) have strong interference from neighboring SBSs and thus the performance of MBSs decreases. Thus, in this paper, we propose a novel intelligent dynamic power control (DPC) with cell range expansion (CRE) to improve the downlink performance of both small-cell user equipments (SUEs) and CRE user equipments (CUEs) in 5G HetNets. That is, in the proposed DPC scheme, each MUE first collects the received signal strength indicator (RSSI) measurements from neighboring SBSs and sends them to the serving MBS. Then, the MBS finds MUEs with strong interference from neighboring SBSs based on a given target threshold of CRE and offloads a fraction of MUEs from MBSs to SBSs. In addition, SBSs divide their SUEs and CUEs into two groups, i.e., inner and outer groups, to assign different subchannels and dynamically allocate the appropriate transmission power to increase the performance of both SUEs and CUEs. Through simulation results, it is shown that the proposed DPC scheme outperforms others in terms of the capacity and outage probability of SUEs and CUEs. Full article
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22 pages, 5392 KiB  
Article
Petrogenesis and Tectonic Setting of the Baluogenguole Mafic Dykes, Zongwulong Belt: Implications for Evolution of the Northern East Paleo-Tethys Ocean
by Min Chen, Tong Pan, Wen-Tao Zhao, Yan-He Wang, Chun-Ji Xue, Bing-Zhang Wang and Chu-Lin Xia
Minerals 2024, 14(5), 473; https://doi.org/10.3390/min14050473 (registering DOI) - 29 Apr 2024
Abstract
The late Paleozoic tectonic setting of the Zongwulong Belt (ZWLB), a significant unit located in the northern Qaidam margin, Qinghai province, remains uncertain. Diabase dykes in the western part of the Zongwulong Belt offer insights into this issue. Field investigations reveal that the [...] Read more.
The late Paleozoic tectonic setting of the Zongwulong Belt (ZWLB), a significant unit located in the northern Qaidam margin, Qinghai province, remains uncertain. Diabase dykes in the western part of the Zongwulong Belt offer insights into this issue. Field investigations reveal that the dips of the dykes are almost vertical, and they have sharp boundaries with the host rocks. These dykes consist of plagioclase, clinopyroxene, and opaque minerals exhibiting a characteristic porphyritic texture and massive structure. Zircon U-Pb dating of the dykes yields a weighted 206Pb/238U age of 289 ± 1 Ma. The dykes exhibit relatively high concentrations of TFeO, K2O + Na2O, and TiO2, while the SiO2 and MgO concentrations are relatively low. They display relative light-over-heavy REE enrichment, and lack negative Nb-Ta and Eu anomalies. The dykes underwent negligible crustal contamination, and experienced extensive fractional crystallization of olivine, clinopyroxene, and Fe-Ti oxides. Originating from the spinel–garnet transition zone at depths of approximately 75 km, the dykes result from garnet facies low-degree melting (5%–10%) in a continental rift setting. Combining these findings with regional geological data, we propose that the ZWLB likely experienced a continental rift in the west and exhibited a narrow oceanic environment in the east in the late Paleozoic period, potentially representing the most distant north branch of the East Paleo-Tethys Ocean. Full article
(This article belongs to the Section Mineral Geochemistry and Geochronology)
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16 pages, 2541 KiB  
Article
Study on Labor Productivity Improvement Based on Situational Awareness and Improved Value Stream Mapping
by Lixuan Jiang, Hongying Shen and Jinhua Zhang
Buildings 2024, 14(5), 1253; https://doi.org/10.3390/buildings14051253 (registering DOI) - 29 Apr 2024
Abstract
This study addresses the issue of poor construction labor productivity, exploring the process and methodology for increasing construction workers’ participation in labor productivity improvement, as well as a situational awareness (SA) development approach for construction workers based on lean construction (LC). A framework [...] Read more.
This study addresses the issue of poor construction labor productivity, exploring the process and methodology for increasing construction workers’ participation in labor productivity improvement, as well as a situational awareness (SA) development approach for construction workers based on lean construction (LC). A framework for enhancing labor productivity through SA has been developed. A case study approach is utilized to describe the LC improvement process, which includes enhancing construction workers’ SA for waste minimization and process optimization and engaging them in LC with improved value stream mapping (VSM). The labor productivity measurement approach, combined with VSM, is used to evaluate the labor productivity improvement with labor productivity improvement rate, resource efficiency, and flow efficiency. The results demonstrate that the labor productivity of the case project has increased by 24.07%, with 11.54% contributing resource efficiency and 88.46% contributing flow efficiency, indicating a greater increase in flow efficiency. This study fills a research gap in terms of the combination of construction workers’ SA enhancement and the involvement of LC practices to boost labor productivity. Furthermore, this study proposes an SA development approach based on LC at three levels: waste perception, VSM implementation, and labor productivity improvement assessment. This provides new perspectives for leveraging construction workers’ SA to increase labor productivity. The improved VSM approach and labor productivity measurements provide an operationalization of workers’ participation in continuous improvement. This research is of great practical relevance for augmenting the competencies of construction laborers and inspiring them to engage in labor productivity enhancement. Full article
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13 pages, 1858 KiB  
Article
Fermented Rapeseed and Soybean Alone and in Combination with Macro Algae Inhibit Human and Pig Pathogenic Bacteria In Vitro
by Frederik Beck, Ninfa Rangel Pedersen and Dennis Sandris Nielsen
Microorganisms 2024, 12(5), 891; https://doi.org/10.3390/microorganisms12050891 (registering DOI) - 29 Apr 2024
Abstract
Higher plants produce secondary metabolites expressing antimicrobial effects as a defense mechanism against opportunistic microorganisms living in close proximity with the plant. Fermentation leads to bioconversion of plant substrates to these bioactive compounds and their subsequent release via breakdown of plant cell walls. [...] Read more.
Higher plants produce secondary metabolites expressing antimicrobial effects as a defense mechanism against opportunistic microorganisms living in close proximity with the plant. Fermentation leads to bioconversion of plant substrates to these bioactive compounds and their subsequent release via breakdown of plant cell walls. Fermented feed products have recently started to become implemented in the pig industry to reduce overall disease pressure and have been found to reduce events such as post-weaning diarrhea. In this study, we investigate the antimicrobial potential of fermented soybean- and rapeseed-based pig feed supplements with and without added seaweed. The antimicrobial effect was tested in a plate well diffusion assay against a range of known human and livestock pathogenic bacteria. Further, we investigate the metabolite profiles based on liquid-chromatography mass-spectrometry (LC-MS) analysis of the fermented products in comparison to their unfermented constituents. We observed a pronounced release of potential antimicrobial secondary metabolites such as benzoic acids when the plant material was fermented, and a significantly increased antimicrobial effect compared to the unfermented controls against several pathogenic bacteria, especially Salmonella enterica Typhimurium, Listeria monocytogenes, Yersinia enterocolitica, and a strain of atopic dermatitis causing Staphylococcus aureus CC1. In conclusion, fermentation significantly enhances the antimicrobial properties of rapeseed, soybean, and seaweed, offering a promising alternative to zinc oxide for controlling pathogens in piglet feed. This effect is attributed to the release of bioactive metabolites effective against pig production-relevant bacteria. Full article
(This article belongs to the Section Food Microbiology)
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20 pages, 4543 KiB  
Review
Role and Applications of Experimental Animal Models of Fontan Circulation
by Zakaria Jalal, Elise Langouet, Nabil Dib, Soazig Le-Quellenec, Mansour Mostefa-Kara, Amandine Martin, François Roubertie and Jean-Benoît Thambo
J. Clin. Med. 2024, 13(9), 2601; https://doi.org/10.3390/jcm13092601 (registering DOI) - 29 Apr 2024
Abstract
Over the last four decades, the Fontan operation has been the treatment of choice for children born with complex congenital heart diseases and a single-ventricle physiology. However, therapeutic options remain limited and despite ongoing improvements in initial surgical repair, patients still experience a [...] Read more.
Over the last four decades, the Fontan operation has been the treatment of choice for children born with complex congenital heart diseases and a single-ventricle physiology. However, therapeutic options remain limited and despite ongoing improvements in initial surgical repair, patients still experience a multiplicity of cardiovascular complications. The causes for cardiovascular failure are multifactorial and include systemic ventricular dysfunction, pulmonary vascular resistance, atrioventricular valve regurgitation, arrhythmia, development of collaterals, protein-losing enteropathy, hepatic dysfunction, and plastic bronchitis, among others. The mechanisms leading to these late complications remain to be fully elucidated. Experimental animal models have been developed as preclinical steps that enable a better understanding of the underlying pathophysiology. They furthermore play a key role in the evaluation of the efficacy and safety of new medical devices prior to their use in human clinical studies. However, these experimental models have several limitations. In this review, we aim to provide an overview of the evolution and progress of the various types of experimental animal models used in the Fontan procedure published to date in the literature. A special focus is placed on experimental studies performed on animal models of the Fontan procedure with or without mechanical circulatory support as well as a description of their impact in the evolution of the Fontan design. We also highlight the contribution of animal models to our understanding of the pathophysiology and assess forthcoming developments that may improve the contribution of animal models for the testing of new therapeutic solutions. Full article
(This article belongs to the Special Issue Management of Pediatric Congenital Heart Disease)
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14 pages, 295 KiB  
Article
Effect of Dialectical Behavior Therapy on Negative Affect, and Symptoms of Depression and Anxiety in Individuals with Borderline Personality Disorder during COVID-19 Pandemic
by Olga Malas and Alba Gómez-Domenech
J. Clin. Med. 2024, 13(9), 2603; https://doi.org/10.3390/jcm13092603 (registering DOI) - 29 Apr 2024
Abstract
Background: This study investigated the effectiveness of dialectical behavior therapy (DBT) in patients with borderline personality disorder (BPD) during the COVID-19 pandemic, assessing negative affect, depression, and anxiety levels as indicators of health. Methods: A total of 287 participants were recruited, [...] Read more.
Background: This study investigated the effectiveness of dialectical behavior therapy (DBT) in patients with borderline personality disorder (BPD) during the COVID-19 pandemic, assessing negative affect, depression, and anxiety levels as indicators of health. Methods: A total of 287 participants were recruited, including BPD patients at different stages of treatment and the general population without a diagnosis of BPD. Questionnaires were used to assess the fear of COVID-19 and the referenced health indicators. Results: No differences were observed between groups in levels of fear of COVID-19, but there were differences in the health indicators studied. BPD patients in long-term treatment showed levels of negative affect similar to those of the general population, while those in early treatment stages exhibited significantly higher levels. However, no significant improvements were observed in levels of depression and anxiety in the long-term treatment group compared to those who underwent the initial treatment phase. Conclusions: These findings underscore the importance of effectively intervening in BPD, especially in stress-inducing situations such as the pandemic, and suggest the need to explore complementary approaches to addressing depression and anxiety in this clinical context. Full article
18 pages, 790 KiB  
Article
Multiplex Detection of Seven Staphylococcal Enterotoxins Using Liquid Chromatography–Mass Spectrometry Combined with a Novel Capture Molecule
by Jing Lv, Tingting Liu, Xinyu Fang, Songyang Han, Lina Dong, Jiaxin Li, Jing Wang, Jinglin Wang, Shan Gao, Lin Kang and Wenwen Xin
Separations 2024, 11(5), 136; https://doi.org/10.3390/separations11050136 (registering DOI) - 29 Apr 2024
Abstract
Food poisoning caused by Staphylococcal enterotoxins (SEs) is prevalent globally, making efficient detection of these toxins very important. Traditionally, liquid chromatography–mass spectrometry required immunosorbent enrichment by magnetic bead-coupled antibodies obtained by animal-specific immunization. However, this method is time-consuming and costly. In this study, [...] Read more.
Food poisoning caused by Staphylococcal enterotoxins (SEs) is prevalent globally, making efficient detection of these toxins very important. Traditionally, liquid chromatography–mass spectrometry required immunosorbent enrichment by magnetic bead-coupled antibodies obtained by animal-specific immunization. However, this method is time-consuming and costly. In this study, two recombinant protein capture molecules were designed based on the principle of toxins binding to Major Histocompatibility Complex (MHCII) and T cell receptor (TCR) molecules. The two capture molecules are called MHCII and MHCII-D10. The design of the MHCII and TCR-D10 was achieved through searching for the binding site protein sequence of Staphylococcal enterotoxins in the relevant literature, and MHCII-D10 was to link MHCII sequence with TCR-D10 sequence using linker (G4S)3 linking peptide. These capture molecules were shown to effectively bind to seven types of toxins and to capture SEs in various matrices. The digestion time, ratio, and temperature were further optimized, reducing the overall digestion time to just 2 h. The specificity, linearity, sensitivity, precision (RSD%), and recovery of the two methods were verified by liquid chromatography–mass spectrometry. When the MHCII and MHCII-D10 captured the toxins, the limit of quantification (LOD) in the 1 × PBS, plasma, and milk matrices ranged from 1.5625 to 100 fmol/µL, with the recovery rate ranging from 18.4% to 96%. The design of these capture molecules eliminates the need for animal-specific immunization, simplifying the pre-detection process and avoiding ethical concerns. This development holds significant promise for clinical diagnosis and reference. Full article
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13 pages, 714 KiB  
Article
Energy Budget Characterisation of the Optimal Disturbance in Stratified Shear Flow
by Larry E. Godwin, Philip M. J. Trevelyan, Takeshi Akinaga and Sotos C. Generalis
Fluids 2024, 9(5), 106; https://doi.org/10.3390/fluids9050106 (registering DOI) - 29 Apr 2024
Abstract
Stratified Taylor–Couette flow (STCF) undergoes transient growth. Recent studies have shown that there exists transient amplification in the linear regime of counter-rotating STCF. The kinetic budget of the optimal transient perturbation is analysed numerically to simulate the interaction of the shear production (SP), [...] Read more.
Stratified Taylor–Couette flow (STCF) undergoes transient growth. Recent studies have shown that there exists transient amplification in the linear regime of counter-rotating STCF. The kinetic budget of the optimal transient perturbation is analysed numerically to simulate the interaction of the shear production (SP), buoyancy flux (BP), and other energy components that contributes to the total optimal transient kinetic energy. These contributions affect the total energy by influencing the perturbation to extract kinetic energy (KE) from the mean flow. The decay of the amplification factor resulted from the positive amplification of both BP and SP, while the growth is attributed to the negative and positive amplification of BP and SP, respectively. The optimal SP is positively amplified, implying that there is the possibility of constant linear growth. These findings agree with the linear growth rate for increasing values of Grashof number. Full article
(This article belongs to the Collection Challenges and Advances in Heat and Mass Transfer)
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13 pages, 259 KiB  
Article
Nutritional Value and Physicochemical Properties of Male and Female Broad-Breasted Bronze Turkey Muscle
by Anna Czech, Piotr Domaradzki, Mateusz Niedzielak and Joanna Stadnik
Foods 2024, 13(9), 1369; https://doi.org/10.3390/foods13091369 (registering DOI) - 29 Apr 2024
Abstract
Colored varieties of turkeys, such as the broad-breasted Bronze, not currently subject to intensive breeding work, are kept only in amateur breeding and treated rather as ornamental poultry. They are raised in extensive systems, which undoubtedly affects the quality of the meat obtained. [...] Read more.
Colored varieties of turkeys, such as the broad-breasted Bronze, not currently subject to intensive breeding work, are kept only in amateur breeding and treated rather as ornamental poultry. They are raised in extensive systems, which undoubtedly affects the quality of the meat obtained. Consumers are looking for meat with specific and unique sensory qualities; hence, the interest in meat from turkeys with a slower-than-typical growth rate, such as the broad-breasted Bronze, is justified. The object of this research was to analyze the physicochemical properties and nutritional value (amino acid, fatty acid, and antioxidant profile) of the breast and thigh muscles of broad-breasted Bronze turkeys with regard to gender. It was shown that gender had little effect on muscle chemical composition, amino acid, and fatty acid content (p > 0.05), as well as most oxido-reduction indices. However, significant differences were noted in muscle quality traits such as color brightness (L*; turkeys > indors; p = 0.023), proportion of red (a*; turkeys < indors; p = 0.048) and yellow (b*; turkeys > indors; p = 0.039), and water absorption (turkeys < indors; p = 0.009). The type of muscle also had a significant effect on quality characteristics. Higher a*, b*, C*, pH, water absorption, and thermal leakage were shown in the femoral muscle (p < 0.001), while L* and h were higher in the pectoral muscle (p < 0.001). Turkey meat was characterized by a high proportion of unsaturated fatty acids (MUFA + PUFA ~68%), favorable FA index values, and low lipid oxidation indices. Thigh muscles (especially turkey) were more caloric, and contained significantly (p < 0.001) more fat and all major FA groups. Breast muscles (especially of turkeys) were characterized by a high protein content (about 25%) and a high proportion of essential amino acids. The data obtained indicate that broad-breasted Bronze turkeys can provide high-quality meat, and are an excellent option for meeting modern consumer needs. Full article
(This article belongs to the Section Meat)

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