Psychostimulants alter cellular morphology and activate neuroimmune signaling in a number of brain regions, yet few prior studies have investigated their persistence beyond acute abstinence or following high levels of voluntary drug intake. In this study, we examined the effects of the repeated binge-like self-administration (96 h/week for 3 weeks) of methamphetamine (METH) and 21 days of abstinence in female and male rats on changes in cell density, morphology, and cytokine levels in two addiction-related brain regions—the prefrontal cortex (PFC) and dorsal striatum (DStr). We also examined the effects of similar patterns of intake of the cocaine-like synthetic cathinone derivative 3,4-methylenedioxypyrovalerone (MDPV) or saline as a control. Robust levels of METH and MDPV intake (~500–1000 infusions per 96 h period) were observed in both sexes. We observed no changes in astrocyte or neuron density in either region, but decreases in dendritic spine densities were observed in PFC pyramidal and DStr medium spiny neurons. The microglial cell density was decreased in the PFC of METH self-administering animals, accompanied by evidence of microglial apoptosis. Changes in microglial morphology (e.g., decreased territorial volume and ramification and increased cell soma volume) were also observed, indicative of an inflammatory-like state. Multiplex analyses of PFC and DStr cytokine content revealed elevated levels of various interleukins and chemokines only in METH self-administering animals, with region- and sex-dependent effects. Our findings suggest that voluntary binge-like METH or MDPV intake induces similar cellular perturbations in the brain, but they are divergent neuroimmune responses that persist beyond the initial abstinence phase. Full article

In the present study, the effectiveness of catalytic wet oxidation triggered by using Cu-Ce/γ-Al₂O₃ to degrade antibiotic excess sludge was investigated, during which some small molecule carboxylic acids were produced, which are valuable in biological wastewater treatment as an organic carbon source. The influence of reaction parameters on the degradation efficiency was explored through single-factor and orthogonal experiments, including catalyst amount, reaction temperature and time, and oxygen supply amount. The results illustrated that the treatment system can achieve 81.2% COD and 93.8% VSS removal rates under optimized reaction conditions. Carboxylic acids produced after the sludge degradation mainly included acetic acid, propanoic acid, etc. The results of wastewater biological treatment experiments exhibited that the degraded solution after catalytic wet oxidation has potential to be used as a carbon source to meet the demand of biological treatment, which helps the removal of COD and TN. This work confirms the effectiveness of catalyst for enhancing antibiotic excess sludge treatment, which provided a new idea for the rational disposal of antibiotic excess sludge. Full article

Prenylated flavonoids (PFs) are natural flavonoids with a prenylated side chain attached to the flavonoid skeleton. They have great potential for biological activities such as anti-diabetic, anti-cancer, antimicrobial, antioxidant, anti-inflammatory, enzyme inhibition, and anti-Alzheimer’s effects. Medicinal chemists have recently paid increasing attention to PFs, which have become vital for developing new therapeutic agents. PFs have quickly developed through isolation and semi- or full synthesis, proving their high value in medicinal chemistry research. This review comprehensively summarizes the research progress of PFs, including natural PFs from the Moraceae family and their pharmacological activities. This information provides a basis for the selective design and optimization of multifunctional PF derivatives to treat multifactorial diseases. Full article

On 21 May 2023, a hidden eruption occurred at the Southeast Crater (SEC) of Etna (Italy); indeed, bad weather prevented its direct and remote observation. Tephra fell toward the southwest, and two lava flows propagated along the SEC’s southern and eastern flanks. The monitoring system of the Istituto Nazionale di Geofisica e Vulcanologia testified to its occurrence. We analyzed the seismic and infrasound signals to constrain the temporal evolution of the fountain, which lasted about 5 h. We finally reached Etna’s summit two weeks later and found an unexpected pyroclastic density current (PDC) deposit covering the southern lava flow at its middle portion. We performed unoccupied aerial system and field surveys to reconstruct in 3D the SEC, lava flows, and PDC deposits and to collect some samples. The data allowed for detailed mapping, quantification, and characterization of the products. The resulting lava flows and PDC deposit volumes were (1.54 ± 0.47) × 10⁶ m³ and (1.30 ± 0.26) × 10⁵ m³, respectively. We also analyzed ground-radar and satellite data to evaluate that the plume height ranges between 10 and 15 km. This work is a comprehensive analysis of the fieldwork, UAS, volcanic tremor, infrasound, radar, and satellite data. Our results increase awareness of the volcanic activity and potential dangers for visitors to Etna’s summit area. Full article

Background. Music evokes positive emotions and reduces stress and anxiety. Operating room (OR) staff face various challenges which can lead to high levels of stress. The aim of the study is to assess whether listening to music during intraoperative phases improves the work environment by reducing anxiety and stress in the entire surgical team. Methods. A prospective observational study was conducted from February to September 2023, involving medical personnel, nursing staff, and nursing students. They were divided into two groups: Group 1 with music during surgical procedures, and Group 2 without music. Participants were administered two validated instruments: the Zung Anxiety Self-Assessment Scale (SAS) to measure anxiety, and the Positive and Negative Affect Schedule to assess emotions generating stress. Additional items were included for demographics, job satisfaction, and the organization method. Results. Music did not impact anxiety, but increased positive emotions while reducing negative ones. Music had an ancillary effect, highlighting the need for significant organizational interventions aimed at increasing operator satisfaction, including offering voluntary instead of mandatory assignments to nursing staff. Conclusions. Music appears to reduce stress in the intraoperative team when supported by a positive work environment in which assigned operators have chosen to work in the OR. Full article

We aimed to analyze the association between CYP7B1 and prostate cancer, along with its association with proteins involved in cancer and metabolic processes. A retrospective analysis was performed on 390 patients with prostate cancer (PC) or benign prostatic hyperplasia (BPH). We investigated the interactions between CYP7B1 expression and proteins associated with PC and metabolic processes, followed by an analysis of the risk of biochemical recurrence based on CYP7B1 expression. Of the 139 patients with elevated CYP7B1 expression, 92.8% had prostate cancer. Overall, no increased risk of biochemical recurrence was associated with CYP7B1 expression. However, in a non-diabetic subgroup analysis, higher CYP7B1 expression indicated a higher risk of biochemical recurrence, with an HR of 1.78 (CI: 1.0–3.2, p = 0.05). PC is associated with elevated CYP7B1 expression. In a subgroup analysis of non-diabetic patients, elevated CYP7B1 expression was associated with an increased risk of biochemical recurrence, suggesting increased cancer aggressiveness. Full article

The results of diagnostic tests under steady-state speed conditions of an unloaded engine do not fully reflect the emissivity of vehicles adapted to run on natural gas. Therefore, it is reasonable to pay attention to the emissions performance of these vehicles under dynamic conditions. In this regard, the tests were carried out on a chassis dynamometer with the engine fueled by gasoline and natural gas. Due to the area of operation of natural gas vehicles being usually limited to urban areas, the urban phases of the NEDC (New European Driving Cycle) and WLTC (Worldwide harmonized Light-duty vehicles Test Cycle) were adapted. While CO₂ emissions are lower when fueled by natural gas, CH₄ emissions can be high, which is related to momentary changes in the composition of the combustible mixture. Although CH₄ emissions are higher when the engine runs on natural gas, the CO_2eq value is, depending on the driving cycle, about 15–25% lower than when running on petrol. Additionally, studies have shown that in engines adapted to run on CNG (compressed natural gas), it is advisable to consider the use of catalytic converters optimized to run on natural gas, as is the case with vehicles which are factory–adapted to run on CNG. Full article

Bacteria in the genus Vibrio are ubiquitous in estuarine and coastal waters. Some species (including Vibrio cholerae and Vibrio vulnificus) are known human pathogens causing ailments like cholera, diarrhea, or septicemia. Notably, V. vulnificus can also cause a severe systemic infection (known as vibriosis) in eels raised in aquaculture facilities. Water samples were periodically collected from the estuary of the Asahi River, located in the southern part of Okayama City, Japan. These samples were directly plated onto CHROMagar Vibrio plates, and colonies displaying turquoise-blue coloration were selected. Thereafter, polymerase chain reaction was used to identify V. cholerae and V. vulnificus. A total of 30 V. cholerae strains and 194 V. vulnificus strains were isolated during the warm season when the water temperature (WT) was higher than 20 °C. Concurrently, an increase in coliforms was observed during this period. Notably, V. vulnificus has two genotypes, designated as genotype 1 and genotype 2. Genotype 1 is pathogenic to humans, while genotype 2 is pathogenic to both humans and eels. The loop-mediated isothermal amplification method was developed to rapidly determine genotypes at a low cost. Of the 194 strains isolated, 80 (41.2%) were identified as genotype 1 strains. Among the 41 strains isolated when the WTs were higher than 28 °C, 25 strains (61.0%) belonged to genotype 1. In contrast, of the 32 strains isolated when the WTs were lower than 24 °C, 27 strains (84.4%) belonged to genotype 2. These results suggest that the distribution of the two genotypes was influenced by WT. Full article

The escalating prevalence of metabolic disorders, notably type 2 diabetes (T2D) and obesity, presents a critical global health challenge, necessitating deeper insights into their molecular underpinnings. Our study integrates proteomics and metabolomics analyses to delineate the complex molecular landscapes associated with T2D and obesity. Leveraging data from 130 subjects, including individuals with T2D and obesity as well as healthy controls, we elucidate distinct molecular signatures and identify novel biomarkers indicative of disease progression. Our comprehensive characterization of cardiometabolic proteins and serum metabolites unveils intricate networks of biomolecular interactions and highlights differential protein expression patterns between T2D and obesity cohorts. Pathway enrichment analyses reveal unique mechanisms underlying disease development and progression, while correlation analyses elucidate the interplay between proteomics, metabolomics, and clinical parameters. Furthermore, network analyses underscore the interconnectedness of cardiometabolic proteins and provide insights into their roles in disease pathogenesis. Our findings may help to refine diagnostic strategies and inform the development of personalized interventions, heralding a new era in precision medicine and healthcare innovation. Through the integration of multi-omics approaches and advanced analytics, our study offers a crucial framework for deciphering the intricate molecular underpinnings of metabolic disorders and paving the way for transformative therapeutic strategies. Full article

An easy-to-implement and cost-effective Fiber Bragg Grating (FBG) sensor interrogation technique based on a ring Erbium-Doped Fiber Laser (EDFL) topology is proposed and experimentally assessed. The FBG sensor is part of the EDFL cavity and must have a central wavelength located within the linear region of the EDF’s amplified spontaneous emission (ASE) spectrum, which occurs at between 1530 and 1540 nm. In this manner, the wavelength-encoded response of the FBG under strain is converted to a linear variation in the laser output power, removing the need for spectrum analysis as well as any limitations from the use of external edge-filtering components. In addition, the laser linewidth is significantly reduced with respect to the FBG bandwidth, thus improving the resolution of the system, whereas its sensitivity can be controlled through pumping power. The performance of the system has been characterized by modeling and experiments for EDFs with different lengths, doping concentrations, and pumping power levels. The influence of mode-hopping in the laser cavity on the resolution and accuracy of the system has also been investigated. Full article

Lipodystrophies (LDs) are rare, complex disorders of the adipose tissue characterized by selective fat loss, altered adipokine profile and metabolic impairment. Sirtuins (SIRTs) are class III NAD⁺-dependent histone deacetylases linked to fat metabolism. SIRT1 plays a critical role in metabolic health by deacetylating target proteins in tissue types including liver, muscle, and adipose. Circulating SIRT1 levels have been found to be reduced in obesity and increased in anorexia nervosa and patients experiencing weight loss. We evaluated circulating SIRT1 levels in relation to fat levels in 32 lipodystrophic patients affected by congenital or acquired LDs compared to non-LD subjects (24 with anorexia nervosa, 22 normal weight, and 24 with obesity). SIRT1 serum levels were higher in LDs than normal weight subjects (mean ± SEM 4.18 ± 0.48 vs. 2.59 ± 0.20 ng/mL) and subjects with obesity (1.7 ± 0.39 ng/mL), whereas they were close to those measured in anorexia nervosa (3.44 ± 0.46 ng/mL). Our findings show that within the LD group, there was no relationship between SIRT1 levels and the amount of body fat. The mechanisms responsible for secretion and regulation of SIRT1 in LD deserve further investigation. Full article

Recently, due to the decrease in labor force, increase in labor costs, and the desire for improved quality of life, research on robots has been actively conducted to address these issues. However, it is currently difficult to find robots that physically interact with humans. The reason is that the actuators of robots do not have a high torque density on their own. To solve this problem, high-torque-density motors, such as proprioceptive actuators, are being researched. However, the torque density is still insufficient for physical interaction with humans, so a motor with higher torque density has been developed. However, high-torque-density motors have the disadvantage of lower speed characteristics due to increased Back EMF levels. Therefore, to address the deterioration of speed characteristics in the developed motor, we applied the independent three-phase winding structure to improve the speed characteristics. Consequently, through comparison with the Y-Connection and D-Connection, we propose the most suitable winding structure for high-torque-density motors intended for physical interaction with humans. Full article

Well-defined and stable property rights play a pivotal role in shaping human economic behavior by averting the tragedy of the commons. This study employs micro-survey data from Heilongjiang Province, China, to empirically investigate the impact and mechanisms of land approval on the adoption of straw returning tstraw-returning technology by farmers. Utilizing the Probit model and mediation and moderation effect testing methods, the findings reveal the following: (1) Land approval significantly promotes the adoption of straw-returning techniques by farmers, with a marginal effect of 0.288. This view is further validated through counterfactual inference constructed using the propensity score matching method. (2) Endowment effects mediate the relationship between land approval and farmers’ adoption of straw-returning technology. (3) Digital skills and farming scale negatively moderate the policy’s impact on farmers’ adoption of straw-returning technology. (4) In terms of control variables, the age of farmers and the dispersion of cultivated land have a significant negative impact on the adoption of straw-returning technology by farmers, while training related to agricultural straw-returning skills and government technology promotion significantly positively affects the use of straw-returning technology by farmers. Therefore, the clarity of land property rights helps to harness the policy effects of land approval and provides a research approach for countries with communal land ownership to implement actions for soil quality conservation. Full article

Pesticides ensure greater productivity in less time; however, they spread beyond the perimeters to which they are applied to reach non-target organisms, thereby affecting plant, animal, and human health. Thiamethoxam (TMX) is considered to be one of the main agents responsible for poisoning bees and potentially contaminating surface and groundwater. Conventional water-treatment protocols are unable to degrade thiamethoxam; therefore, electrochemically advanced oxidative processes (EAOPs) have become promising alternatives owing to their ease of operation and cost-effectiveness. Herein, we examined the use of EAOPs to oxidize thiamethoxam in commercial Actara^® and analyzed treatment efficiencies through phytotoxicity studies using cucumber and maize seeds as bioindicators. In addition, the cost of each process was analyzed based on the resulting current efficiency. The treated solutions were used to germinate seeds that were analyzed for total protein, hydrogen peroxide, lipid peroxidation (MDA), superoxide dismutase (SOD), and catalase (CAT) activities. EAOPs were found to effectively oxidize TMX, with more than 50% degraded and 80% COD removed under all treatment conditions, even when the commercial product was used. The photoelectro-Fenton process using 10 mg L⁻¹ FeSO4 and 100 mg L⁻¹ H₂O₂ exhibited the best results, with 79% of the TMX degraded and 83% of the COD removed, additionally exhibiting the lowest estimated operating cost (USD 1.01 dm⁻³). Higher enzymatic SOD and CAT activities, total protein content, and H₂O₂ concentration were observed; however, no significant changes in MDA were recorded. This treatment protocol effectively oxidizes TMX and reduces its phytotoxicity in maize and cucumber seedlings. Full article

Density functional theory (DFT) calculations of hydrogen adsorption on titanium nitride had previously shown that hydrogen may adsorb on both titanium and nitrogen sites with a moderate adsorption energy. Further, the diffusion barrier was also found to be low. These findings may qualify TiN, a versatile multifunctional material with electronic conductivity, as an electrode material for the hydrogen evolution reaction (HER). This was the main impetus of this study, which aims to experimentally and theoretically investigate the electrocatalytic properties of TiN layers that were processed on a Ti substrate using reactive ion sputtering. The properties are discussed, focusing on the role of oxygen defects introduced during the sputtering process on the HER. Based on DFT calculations, it is shown that these oxygen defects alter the electronic environment of the Ti atoms, which entails a low hydrogen adsorption energy in the range of −0.1 eV; this leads to HER performances that match those of Pt-NPs in acidic media. When a few nanometer-thick layers of Pd-NPs are sputtered on top of the TiN layer, the performance is drastically reduced. This is interpreted in terms of oxygen defects being scavenged by the Pd-NPs near the surface, which is thought to reduce the hydrogen adsorption sites. Full article

The wide size range and high tendency to agglomerate of in-situ TiB₂ particles in reinforced Al matrix composites introduce great difficulties in their size characterization. In order to use a nanoparticle size analyzer (NSA) to obtain the precise size distribution of TiB₂ particles, a controlled size characterization process has been explored. First, the extraction and drying processes for TiB₂ particles were optimized. In the extraction process, alternated applications of magnetic stirring and normal ultrasound treatments were proven to accelerate the dissolution of the Al matrix in HCl solution. Furthermore, freeze-drying was found to minimize the agglomeration tendency among TiB₂ particles, facilitating the acquisition of pure powders. Such powders were quantitatively made into an initial TiB₂ suspension. Second, the chemical and physical dispersion technologies involved in initial TiB₂ suspension were put into focus. Chemically, adding PEI (M.W. 10000) at a ratio of m_PEI/m_TiB2 = 1/30 into the initial suspension can greatly improve the degree of TiB₂ dispersion. Physically, the optimum duration for high-energy ultrasound application to achieve TiB₂ dispersion was 10 min. Overall, the corresponding underlying dispersion mechanisms were discussed in detail. With the combination of these chemical and physical dispersion specifications for TiB₂ suspension, the bimodal size distribution of TiB₂ was able to be characterized by NSA for the first time, and its number-average diameter was 111 ± 6 nm, which was reduced by 59.8% over the initial suspension. Indeed, the small-sized and large-sized peaks of the TiB₂ particles characterized by NSA mostly match the results obtained from transmission electron microscopy and scanning electron microscopy, respectively. Full article

The reliance on more sustainable refrigeration systems with less electricity consumption attracts a lot of attention as the demand for refrigeration increases due to population growth and global warming threats. This study examines the use of a cascade vapor-compression/adsorption refrigeration system in hot weather, focusing on condensing temperatures of 50, 55, and 60 °C, whereas an air-cooled condenser is in use due to practical considerations. A fully coupled transient model is developed using COMSOL Multiphysics to simulate the integrated system, considering the practical limitations of the vapor compression system (VCS) and the dynamic nature of the adsorption system (ADS). The model combines a lumped model for the ADS with the manufacturer’s data for a VCS compressor at different condensing and evaporating temperatures. It was found that the VCS is more sensitive to the change in the ADS’s condensing temperature, since when the temperature is raised from 50 °C to 60 °C, the VCS’s COP decreases by 29.5%, while the ADS’s COP decreases by 7.55%. Furthermore, the cycle time of ADS plays an important role in providing the cooling requirements for the bottoming cycle (VCS), and it can be optimized to maximize the energy conversion efficiency of the VCS. At optimum cycle time and compared to the conventional VCS, the cascade system can boost the cooling capacity of the VCS by 18.2%, lower the compressor power by 63.2%, and greatly enhance the COP by 221%. These results indicate that the application of the cascade VCS/ADS in such severe conditions is a more sustainable and energy-efficient solution to meet the growing need for refrigeration. Full article

The rapid and accurate detection of forest disturbances in temperate forests has become increasingly crucial as policy demands and climate pressure on these forests rise. The cloud-penetrating Sentinel-1 radar constellation provides frequent and high-resolution observations with global coverage, but few studies have assessed its potential for mapping disturbances in temperate forests. This study investigated the sensitivity of temporally dense C-band backscatter data from Sentinel-1 to varying management-related disturbance intensities in temperate forests, and the influence of confounding factors such as radar backscatter signal seasonality, shadow, and layover on the radar backscatter signal at a pixel level. A unique network of 14 experimental sites in the Netherlands was used in which trees were removed to simulate different levels of management-related forest disturbances across a range of representative temperate forest species. Results from six years (2016–2022) of Sentinel-1 observations indicated that backscatter seasonality is dependent on species phenology and degree of canopy cover. The backscatter change magnitude was sensitive to medium- and high-severity disturbances, with radar layover having a stronger impact on the backscatter disturbance signal than radar shadow. Combining ascending and descending orbits and complementing polarizations compared to a single orbit or polarization was found to result in a 34% mean increase in disturbance detection sensitivity across all disturbance severities. This study underlines the importance of linking high-quality experimental ground-based data to dense satellite time series to improve future forest disturbance mapping. It suggests a key role for C-band backscatter time series in the rapid and accurate large-area monitoring of temperate forests and, in particular, the disturbances imposed by logging practices or tree mortality driven by climate change factors. Full article

The aim of this study was to develop microcapsules containing juniper or black pepper essential oils, using a combination of faba bean protein and chia seed polysaccharides (in ratios of 1:1, 1:2, 2:1). By synergizing these two polymers, our goal was to enhance the efficiency of essential oil microencapsulation, opening up various applications in the food industry. Additionally, we aimed to investigate the influence of different polymer mixing ratios on the properties of the resulting microcapsules and the course of the complex coacervation process. To dissolve the essential oils and limit their evaporation, soybean and rapeseed oils were used. The powders resulting from the freeze-drying of coacervates underwent testing to assess microencapsulation efficiency (65.64–87.85%), density, flowability, water content, solubility, and hygroscopicity. Additionally, FT-IR and DSC analyses were conducted. FT-IR analysis confirmed the interactions between the components of the microcapsules, and these interactions were reflected in their high thermal resistance, especially at a protein-to-polysaccharide ratio of 2:1 (177.2 °C). The water content in the obtained powders was low (3.72–7.65%), but it contributed to their hygroscopicity (40.40–76.98%). Full article

Measurement-based care (MBC) is a procedure in which systematic and routine assessments are performed. Through this practice, clinicians can verify the progress of the symptomatology of the patient and adapt the appointments and the intervention to the current symptoms. Studies have reflected on the importance and the benefits of this type of procedure in the adult population, and have shown positive results. However, there is a lack of evidence concerning the remaining populations. Regarding youth, for instance, few articles have evaluated the benefits of using this procedure in clinical practice. However, research focused on this topic has revealed positive results, especially when clinicians were loyal to the MBC procedures. Still, further research is needed. This letter aims to share the methodology used by our multidisciplinary team, composed of psychologists and psychiatrists, in a clinical context at the Hospital Cruz Vermelha, Lisboa, applied to the adult population; the objective is to share and discuss some alterations that could be made to our evaluation protocol to enable the same to be used with the youth population. We believe that implementing MBC for youth is crucial for several reasons, including enhanced treatment efficacy, more personalized treatment, a reduced reliance on subjectivity, and empowerment not only of patients but also families. Full article

Joining high strength 2xxx series aluminum is known to be complex and difficult; these alloys are traditionally considered non-weldable for fusion welding. This paper describes details on welding AA2029-T8 for skin-stiffened structures using refill friction stir spot welding (RFSSW). RFSSW is a solid-state process invented in the early 2000s that produces spot welds that are strong, lightweight, flush, and hermetic. Cycle times between 1 and 3 s are discussed, and process forces within a range of 8 to 14 kN are demonstrated. Furthermore, lap-shear quasi-static tensile strengths are shown to be between 10 kN and 12 kN in 9 mm diameter spots. A comparison of the performance of RFSSW welds made with various tool materials—which include H13 tool steel, tungsten carbide, and MP159—is detailed. Comparisons of parameters, weld consolidation, and heat-affected zones are presented with discussion related to heat generation specific to each tool material. Full article