Coxiellosis or Q fever is an infectious zoonotic disease caused by the bacterium Coxiella burnetii. A systematic review using bibliographic research was carried out, and the focus was the relationship between C. burnetii infection and reproductive disorders in cattle [abortion/stillbirth/perinatal morality/weak calves (ASPW complex); retained foetal membranes (RFMs); metritis/endometritis; and infertility/sub-fertility]. The bibliographical search yielded 443 results from databases, but only 61 were deemed eligible. For each disorder, summary tables were prepared, and a scientific evidence score was calculated for each study based on four criteria to help assess the level of evidence for the impact of C. burnetii on the reproductive disorders assessed: type of publication (peer-reviewed or other); type of study (case–control/cohort or other); type of C. burnetii test (direct or indirect); and comparative statistical analysis (yes or no). In addition, summary tables also included information on the study population, country, authors and year of publication, key findings and an assessment of the evidence for an association. For the ASPW complex, RFMs, metritis/endometritis and infertility/sub-fertility, 43, 9, 8 and 19 studies provided data, respectively. On a scale of four, nearly 50% of all study citations had evidence scores of three or four. For ASPW, RFMs and infertility/sub-fertility, there is a significant body of evidence to support a deleterious role for Q fever. In contrast, for metritis/endometritis, the evidence is unclear. It is concluded that there is a substantial need for further research, particularly involving larger animal populations in more controlled settings. To provide more consistency, it is recommended that authors follow more precise definitions of reproductive parameters and more robust diagnostic methodologies. Full article

Panax notoginseng is a highly regarded medicinal plant that has obstacles associated with continuous cropping. Understanding soil microorganisms is crucial, as they play a major role in this regard. However, soil microorganisms are affected by multiple factors; therefore, we need to conduct more in-depth research. This study investigated the combined effects of irrigation and microbial fertilizer treatments (J1F1, J1F2, J2F1, J2F2, J3F1, J3F2, and CK) on the diversity of bacterial and fungal microbial communities in the rhizosphere of Panax notoginseng. The bacterial 16S rRNA genes and fungal internal transcribed spacer (ITS) sequences were sequenced using Illumina HiSeq. The results showed that, without microbial fertilizer (CK), the microbial community abundance and diversity were significantly lower than in the other treatments; moreover, among the microbial fertilizer treatments, the microbial abundance in F1 was higher than that in F2. Under the same microbial fertilizer application, the incidence rate of Panax notoginseng root rot was J2 > J1 > J3, and the yield of Panax notoginseng was J3 > J2 > J1. Under the same irrigation conditions, the incidence rate of Panax notoginseng root rot was F1 > F2, and the yield of Panax notoginseng was F2 > F1. This study provides important guidance for Panax notoginseng plant microbiota and sustainable agriculture. Full article

Accurate and fast recognition of vehicle license plates from natural scene images is a crucial and challenging task. Existing methods can recognize license plates in simple scenarios, but their performance degrades significantly in complex environments. A novel license plate detection and recognition model YOLOv5-PDLPR is proposed, which employs YOLOv5 target detection algorithm in the license plate detection part and uses the PDLPR algorithm proposed in this paper in the license plate recognition part. The PDLPR algorithm is mainly designed as follows: (1) A Multi-Head Attention mechanism is used to accurately recognize individual characters. (2) A global feature extractor network is designed to improve the completeness of the network for feature extraction. (3) The latest parallel decoder architecture is adopted to improve the inference efficiency. The experimental results show that the proposed algorithm has better accuracy and speed than the comparison algorithms, can achieve real-time recognition, and has high efficiency and robustness in complex scenes. Full article

We study the properties of the constructive linear programming problems. The parameters of linear functions in such problems are constructive real numbers. Solving such a problem involves finding the optimal plan with the constructive real number components. We show that it is impossible to have an algorithm that solves an arbitrary constructive real programming problem. Full article

Gas bearings have the advantages of small friction loss, wide applicable speed range, no pollution, etc., and have important application prospects in micro and small high-speed rotating machinery. However, due to its compressibility and low viscosity, its dynamic stability in high-speed rotating machinery is the key to constraining its development. The experimental study of shaft system dynamics is the main means to explore the mechanism of rotor behavior. On the test platform of dynamic characteristics of multi-disc rotor system supported by aerostatic bearings, experimental research on the nonlinear dynamic characteristics of a rotor system was carried out, and nonlinear vibration test and analysis methods, such as axial orbits, bifurcation diagrams, and spectral characteristics, were adopted, and vibration phenomena, including the critical rotational speed accumulating energy and low-frequency accumulating energy, were presented and the vibration characteristics of bearing fracture faults were presented. The bearing supply pressure and rubber damping pad were introduced as a method to suppress the low-frequency vibration of the aerostatic bearing rotor system, and its vibration-reduction effect was verified by experiments. The above results can provide technical support for vibration control and fault diagnosis of rotor systems supported by aerostatic bearings. Full article

Despite the legalization of psilocybin therapy for depression in terminal illnesses such as advanced cancer through Oregon’s Measure 109 in 2020, significant challenges have impeded its implementation. This review synthesizes the empirical data supporting the utilization of psilocybin therapy for addressing cancer-related depression, including an evaluation of its purported benefits and potential adverse effects. It provides a comprehensive examination of therapeutic strategies, dosing regimens, and barriers to ensuring responsible and equitable access. Salient issues explored include the development of ethical protocols, integration within healthcare systems, ensuring statewide availability, resolving legal ambiguities, and defining clinical standards. Oregon’s pioneering role serves as a case study, highlighting the necessity of addressing regulatory, logistical, and ethical obstacles to ensure the establishment of rigorous and equitable psilocybin care models. Full article

The primary targets of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the lungs are type I pneumocytes, macrophages, and endothelial cells. We aimed to identify lung cells targeted by SARS-CoV-2 using viral nucleocapsid protein staining and morphometric features on patients with fatal COVID-19. We conducted a retrospective analysis of fifty-one autopsy cases of individuals who tested positive for SARS-CoV-2. Demographic and clinical information were collected from forensic reports, and lung tissue was examined for microscopic lesions and the presence of specific cell types. Half of the evaluated cohort were older than 71 years, and the majority were male (74.5%). In total, 24 patients presented diffuse alveolar damage (DAD), and 50.9% had comorbidities (56.9% obesity, 33.3% hypertension, 15.7% diabetes mellitus). Immunohistochemical analysis showed a similar pattern of infected macrophages, infected type I pneumocytes, and endothelial cells, regardless of the presence of DAD (p > 0.5). The immunohistochemical reactivity score (IRS) was predominantly moderate but without significant differences between patients with and without DAD (p = 0.633 IRS for type I pneumocytes, p = 0.773 IRS for macrophage, and p = 0.737 for IRS endothelium). The nucleus/cytoplasm ratio shows lower values in patients with DAD (median: 0.29 vs. 0.35), but the difference only reaches a tendency for statistical significance (p = 0.083). Our study confirms the presence of infected macrophages, type I pneumocytes, and endothelial cells with a similar pattern in patients with and without diffuse alveolar damage. Full article

Draining peatlands to create agricultural land has been the norm in Europe, but in the context of climate change and the loss of biodiversity, these rich ecosystems may reactivate their functions as greenhouse gas sinks and retreat spaces for animals and plants. Against this background, the National Moor Rewetting Strategy was put into effect in Germany in 2023, together with the Natural Climate Protection Action Plan. This article examines the methodology of peatland rewetting from scientific, administrative, social, and technical perspectives. The article focuses on an example of moor rewetting in central Germany: the Rathsbruch moor near the municipality of Zerbst, Saxony-Anhalt. To illustrate the importance of rewetting projects for degraded peatlands, five scenarios with different target soil water levels were considered, and the associated greenhouse gas emissions were calculated for a period of five years. For the planning solution, an estimate of the medium-to-long-term development of the habitat types was made based on current use and the dynamics typical of the habitat. The results for the Rathsbruch moor area showed that increasing the water level in steps of 1, 0.8, or 0.5 m has no significant influence on reducing the CO₂ emissions situation, while a depth of 0.3 m has a slight influence. When the water was raised to 0.1 m below the surface (Scenario 5), a significant CO₂ reduction was observed. The calculated avoided CO₂ costs due to environmental damage show that the environmental benefits multiply with every decimeter of water level increase. The rising groundwater levels and extensification favor the establishment of local biotopes. This means that two of the biggest man-made problems (extinction of species and climate change) can be reduced. Therefore, this research is applicable to the development and planning of recultivation work at municipal and regional levels in Germany and beyond within the framework of EU restoration policy. Full article

This work aimed to elucidate how O₃ pollution causes a loss of regulation in the immune response in both the brain and the intestine. In this work, we studied the effect of exposing rats to low doses of O₃ based on the association between the antioxidant response of superoxide dismutase (SOD) levels and the nuclear factor kappa light chains of activated B cells (NFκB) as markers of inflammation. Method: Seventy-two Wistar rats were used, divided into six groups that received the following treatments: Control and 7, 15, 30, 60, and 90 days of O₃. After treatment, tissues were extracted and processed using Western blotting, biochemical, and immunohistochemical techniques. The results indicated an increase in 4-hydroxynonenal (4HNE) and Cu/Zn-SOD and a decrease in Mn-SOD, and SOD activity in the substantia nigra, jejunum, and colon decreased. Furthermore, the translocation of NFκB to the nucleus increased in the different organs studied. In conclusion, repeated exposure to O₃ alters the regulation of the antioxidant and inflammatory response in the substantia nigra and the intestine. This indicates that these factors are critical in the loss of regulation in the inflammatory response; they respond to ozone pollution, which can occur in chronic degenerative diseases. Full article

In calcium nephrolithiasis (CaNL), most calcium kidney stones are identified as calcium oxalate (CaOx) with variable amounts of calcium phosphate (CaP), where CaP is found as the core component. The nucleation of CaP could be the first step of CaP+CaOx (mixed) stone formation. High urinary supersaturation of CaP due to hypercalciuria and an elevated urine pH have been described as the two main factors in the nucleation of CaP crystals. Our previous in vivo findings (in mice) show that transient receptor potential canonical type 3 (TRPC3)-mediated Ca²⁺ entry triggers a transepithelial Ca²⁺ flux to regulate proximal tubular (PT) luminal [Ca²⁺], and TRPC3-knockout (KO; -/-) mice exhibited moderate hypercalciuria and microcrystal formation at the loop of Henle (LOH). Therefore, we utilized TRPC3 KO mice and exposed them to both hypercalciuric [2% calcium gluconate (CaG) treatment] and alkalineuric conditions [0.08% acetazolamide (ACZ) treatment] to generate a CaNL phenotype. Our results revealed a significant CaP and mixed crystal formation in those treated KO mice (KOT) compared to their WT counterparts (WTT). Importantly, prolonged exposure to CaG and ACZ resulted in a further increase in crystal size for both treated groups (WTT and KOT), but the KOT mice crystal sizes were markedly larger. Moreover, kidney tissue sections of the KOT mice displayed a greater CaP and mixed microcrystal formation than the kidney sections of the WTT group, specifically in the outer and inner medullary and calyceal region; thus, a higher degree of calcifications and mixed calcium lithiasis in the kidneys of the KOT group was displayed. In our effort to find the Ca²⁺ signaling pathophysiology of PT cells, we found that PT cells from both treated groups (WTT and KOT) elicited a larger Ca²⁺ entry compared to the WT counterparts because of significant inhibition by the store-operated Ca²⁺ entry (SOCE) inhibitor, Pyr6. In the presence of both SOCE (Pyr6) and ROCE (receptor-operated Ca²⁺ entry) inhibitors (Pyr10), Ca²⁺ entry by WTT cells was moderately inhibited, suggesting that the Ca²⁺ and pH levels exerted sensitivity changes in response to ROCE and SOCE. An assessment of the gene expression profiles in the PT cells of WTT and KOT mice revealed a safeguarding effect of TRPC3 against detrimental processes (calcification, fibrosis, inflammation, and apoptosis) in the presence of higher pH and hypercalciuric conditions in mice. Together, these findings show that compromise in both the ROCE and SOCE mechanisms in the absence of TRPC3 under hypercalciuric plus higher tubular pH conditions results in higher CaP and mixed crystal formation and that TRPC3 is protective against those adverse effects. Full article

The specific sensory properties attributed to distillates from different plum varieties are intricately linked to aromatic substances, fruit quality, and technology employed during production. This study compares four plum brandies, each made from a renowned plum variety: Presenta, Valjevka, Čačanská lepotica, and Čačanská rodná on a production scale. Analytical and sensory profiles were assessed using GC-FID, an available analytical method advantageous for monitoring industrial fruit distillate production. Between 71 and 85 analytes were detected in the distillates, with the Presenta plum distillate containing the highest number of substances. Statistically significant differences in analyte concentration between plum varieties (p < 0.05) were observed for 11 analytes. The comparison of analytical profiles and sensory evaluation revealed that a high concentration of 1-propanol, despite its negative sensory perception, significantly impacts the overall perception of a distillate, contrasting with substances like acetaldehyde and propyl acetate, which have positive sensory evaluations but lesser significance in content. Our work identified key compounds and procedures that can be used as benchmarks for production of plum brandy with positive sensory evaluation. These findings demonstrate the broad application potential of GC-FID in fruit distillate production as an independent tool for aromatic profile assessment and quality control. Full article

The complex epidemiology of hepatitis C virus (HCV) infection among human immunodeficiency virus (HIV) patients in West Mexico remains poorly understood. Thus, this study aimed to investigate the HCV prevalence, HCV-associated risk factors, and HCV genotypes/subtypes and assess their impacts on liver fibrosis in 294 HIV patients (median age: 38 years; 88.1% male). HCV RNA was extracted and amplified by PCR. Hepatic fibrosis was assessed using three noninvasive methods: transient elastography (TE), the aspartate aminotransferase (AST)-to-platelets ratio index score (APRI), and the fibrosis-4 score (FIB4). Patients with liver stiffness of ≥9.3 Kpa were considered to have advanced liver fibrosis. HCV genotypes/subtypes were determined by line probe assay (LiPA) or Sanger sequencing. The prevalence of HIV/HCV infection was 36.4% and was associated with injection drug use (odds ratio (OR) = 13.2; 95% confidence interval (CI) = 5.9–33.6; p < 0.001), imprisonment (OR = 3.0; 95% CI = 1.7–5.4; p < 0.001), the onset of sexual life (OR = 2.6; 95% CI = 1.5–4.5; p < 0.001), blood transfusion (OR = 2.5; 95% CI = 1.5–4.2; p = 0.001), tattooing (OR = 2.4; 95% CI = 1.4–3.9; p = 0.001), being a sex worker (OR = 2.3; 95% CI = 1.0–5.4; p = 0.046), and surgery (OR = 1.7; 95% CI = 1.0–2.7; p = 0.042). The HCV subtype distribution was 68.2% for 1a, 15.2% for 3a, 10.6% for 1b, 3.0% for 2b, 1.5% for 2a, and 1.5% for 4a. The advanced liver fibrosis prevalence was highest in patients with HIV/HCV co-infection (47.7%), especially in those with HCV subtype 1a. CD4+ counts, albumin, direct bilirubin, and indirect bilirubin were associated with liver fibrosis. In conclusion, HCV infection had a significant impact on the liver health of Mexican HIV patients, highlighting the need for targeted preventive strategies in this population. Full article

As an environmentally friendly natural polymer, citric acid-modified chitosan (CAMC) can effectively regulate the hydration and exothermic processes of cement-based materials. However, the influence of CAMC on the macroscopic properties of concrete and the optimal dosage are still unclear. This work systematically investigates the effects of CAMC on the mixing performance, mechanical properties, shrinkage performance, and durability of concrete. The results indicated that CAMC has a thickening effect and prolongs the setting time of concrete. CAMC has a negative impact on the early strength of concrete, but it is beneficial for the development of the subsequent strength of concrete. With the increase in CAMC content, the self-shrinkage rate of concrete samples decreased from 86.82 to 14.52 με. However, the CAMC-0.6% sample eventually expanded, with an expansion value of 78.49 με. Moreover, the long-term drying shrinkage rate was decreased from 551.46 to 401.94 με. Furthermore, low-dose CAMC can significantly reduce the diffusion coefficient of chloride ions, improve the impermeability and density of concrete, and thereby enhance the freeze–thaw cycle resistance of concrete. Full article

The light weight and excellent mechanical properties of rubber foam means that it is widely applied in the aerospace, automobile, and military industries. However, its poor wear resistance contributes directly to a short service life and a waste of resources. Therefore, the design and development of high-wear-resistance rubber foam are of great importance. In this work, some nanoclay/rubber composite foams were prepared by blending NR/EPDM with different kinds of nanoclays containing layered double hydroxide (LDH), montmorillonite (MMT), and attapulgite (ATP) to indicate the effects of the kinds of nanoclays on the wear resistance and mechanical properties of nanoclay/rubber composite foams. The kinds of nanoclay/rubber composite foams were investigated by Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction. The results showed that nanoclay has heterogeneous nucleation in composite foamed materials. The wear resistance of the composite foam materials with added nanoclay was significantly improved, and the MMT of the lamellar structure (increased by 43.35%) and LDH (increased by 38.57%) were significantly higher than the ATP of the rod-like structure (increased by 13.04%). The improvement in the wear resistance of the matrix was even higher. Compared with other foams, the wear resistance of the OMMT–NR/EPDM foam (increased by 58.89%) with a lamellar structure had the best wear resistance. Due to the increase in the lamellar spacing of the modified OMMT, the exfoliation of worn rubber molecular chains has little effect on the adjacent molecular chains, which prevents the occurrence of crimp wear and further improves the wear resistance of composite foaming materials. Therefore, this work lays the foundation for the manufacturing of rubber foams for wear-resistant applications. Full article

To enhance path tracking precision in intelligent vehicles, this study proposes a lateral–longitudinal control strategy optimized with a Backpropagation (BP) neural network. The strategy employs the BP neural network to dynamically adjust prediction and control time-domain parameters within an established Model Predictive Control (MPC) framework, effectively computing real-time front-wheel steering angles for lateral control. Simultaneously, it integrates an incremental Proportional–Integral–Derivative (PID) approach with a meticulously designed acceleration–deceleration strategy for accurate and stable longitudinal speed tracking. The strategy’s efficiency and superior performance are validated through a comprehensive CarSim(2020)/Simulink(2020b) simulation, demonstrating that the proposed controller adeptly modulates control parameters to adapt to various road adhesion coefficients and vehicle speeds. This adaptability significantly improves tracking and driving dynamics, thereby enhancing accuracy, safety, stability, and real-time responsiveness in the intelligent vehicle tracking control system. Full article

Feeding is a critical process in aquaculture, as it has a direct impact on the quantity and quality of fish. With advances in convolutional neural network (CNN) and vision transformer (ViT), intelligent feeding has been widely adopted in aquaculture, as the real-time monitoring of fish behavior can lead to better feeding decisions. However, existing models still have the problem of insufficient accuracy in the fish behavior-recognition task. In this study, the largemouth bass (Micropterus salmoides) was selected as the research subject, and three categories (weakly, moderately, and strongly hungry) were defined. We applied the deformable attention to the vision transformer (DeformAtt-ViT) to identify the fish hunger degree. The deformable attention module was extremely powerful in feature extraction because it improved the fixed geometric structure of the receptive fields with data-dependent sparse attention, thereby guiding the model to focus on more important regions. In the experiment, the proposed DeformAtt-ViT was compared with the state-of-the-art transformers. Among them, DeformAtt-ViT achieved optimal performance in terms of accuracy, F1-score, recall, and precision at 95.50%, 94.13%, 95.87%, and 92.45%, respectively. Moreover, a comparative evaluation between DeformAtt-ViT and CNNs was conducted, and DeformAtt-ViT still dominated the others. We further visualized the important pixels that contributed the most to the classification result, enabling the interpretability of the model. As a prerequisite for determining the feed time, the proposed DeformAtt-ViT could identify the aggregation level of the fish and then trigger the feeding machine to be turned on. Also, the feeding machine will stop working when the aggregation disappears. Conclusively, this study was of great significance, as it explored the field of intelligent feeding in aquaculture, enabling precise feeding at a proper time. Full article

Tuberculosis (TB) continues to be a major global health challenge and a leading cause of death from infectious diseases. Inspired by the results from a previous work by our group on antimycobacterial N-alkylnitrobenzamides, which are structurally related to the nitrobenzamide family of decaprenylphosphoryl-β-d-ribose oxidase (DprE1) inhibitors, the present study explored a broad array of substituted benzamides. We particularly focused on previously unexplored 3,5-dinitrobenzamide derivatives. Starting with 3,5-dinitrobenzoic acid, we synthesized a diverse library of amides, incorporating both linear and cyclic amine moieties and also assessed the impact of terminal aromatic groups connected through ether, ester, or amide bonds on the bioactivity of the compounds. The synthesis primarily utilized nucleophilic addition/elimination, S_N2, and Mitsunobu reactions. The activity was impacted mainly by two structural features, the addition of an aromatic moiety as a terminal group and the type of linker. The most interesting compounds (c2, d1, and d2, MIC = 0.031 μg/mL) exhibited activities against Mycobacterium Tuberculosis (Mtb) H37Rv comparable to isoniazid. Complementary computational studies helped elucidate potential interactions with DprE1, enhancing our understanding of the molecular basis of their action. Our findings suggest that the most active compounds provide a promising foundation for the continued development of new antimycobacterial agents. Full article

This paper presents an investigation of the frictional behaviour of three-piece piston oil control rings. A bespoke tribometer replicates the kinematics of the contact between a full oil control ring and the cylinder liner. The three-piece oil control ring is composed of two segments, separated by a waveform-type expander. The experimental results indicate the dominance of a mixed regime of lubrication throughout the stroke. This is particularly the case when the experiments are conducted at 80 °C, a typical engine sump temperature, when compared with those at 20 °C (a typical engine start-up temperature in the UK in the summer). A mixed hydrodynamic analytical model of the oil control ring–cylinder liner tribological interface is employed to apportion frictional contributions with their physical underlying mechanisms. Therefore, combined numerical and experimental investigations are extended to lubricant contamination/dilution by ethanol-based fuels. This study shows that the transition from E10 to E85 would have an insignificant effect on the friction generated in the oil control ring conjunction. This holistic approach, using a detailed predictive l mixed regime of lubrication model and a representative bespoke tribometry, has not hitherto been reported in the open literature. Full article

Influenza viruses are constantly evolving and are therefore monitored worldwide in the hope to reduce the burden of disease by annual updates to vaccine recommendations. We conducted genomic sequencing of 110 influenza A and 30 influenza B viruses from specimens collected between October 2023 and February 2024 in Arizona, USA. We identified mutations in the hemagglutinin (HA) antigenic sites as well as the neuraminidase (NA) gene in our samples. We also found no unique HA and NA mutations in vaccinated yet influenza-infected individuals. Real-time genomic sequencing surveillance is important to ensure influenza vaccine effectiveness. Full article

Background: Vitamin D and protein deficiencies are common conditions in the general population. In Orthopaedic surgery, they can result in wound complications or poor bone healing. The goal of this study was, therefore, to determine the prevalence of vitamin D and albumin deficiencies in patients scheduled for elective Orthopaedic procedures. Methods: We performed an observational, noninterventional study using the demographic characteristics via means chart analysis of in-patients with an elective surgery in a single Swiss Orthopaedic centre. The following variables were collected and analysed: age, gender, BMI, ASA score, rate of vitamin D supplementation before surgery, and serum preoperative levels of vitamin D, albumin, Haemoglobin, calcium, and phosphate. Results: A total of 336 patients were analysed; there were 218 women (64.9%) and 118 men (35.1%). The average age was 59.4 years (17–89 years). The average BMI was 26.8 kg/m² (17.8–37.6) and the average ASA score was II (I–III). The overall prevalence of vitamin D deficiency was 82.1%, being more common in the male (89.8%) than female patients (77.9%). Patients who received vitamin D supplements before surgery had an average vitamin D level in the normal range. Of the subgroup of 170 patients who were over 60 years of age, 78.8% of the patients were Vitamin D deficient, with deficiency once again being more common in men (93.3%) than in women (92%). Albumin deficiency was documented in 58.9% of the cases. A total of 62.8% of all the female patients were albumin deficient, and 51.7% of all male patients were. Of the subgroup of 170 patients who were over 60 years of age, 71.8% of the patients were albumin deficient, with the deficiency being practically identical in men (71.1%) and women (72%). Conclusions: Despite increased awareness in the medical community, vitamin D and albumin deficiencies remain highly prevalent in elective Orthopaedic patients. Pre/perioperative screening and addressing possible vitamin D and albumin deficiencies are of great importance for good outcomes in Orthopaedic surgery. Full article