The 2023 MDPI Annual Report has
been released!
 
25 pages, 9712 KiB  
Article
Comparative Analysis of Color Space and Channel, Detector, and Descriptor for Feature-Based Image Registration
by Wenan Yuan, Sai Raghavendra Prasad Poosa and Rutger Francisco Dirks
J. Imaging 2024, 10(5), 105; https://doi.org/10.3390/jimaging10050105 (registering DOI) - 28 Apr 2024
Abstract
The current study aimed to quantify the value of color spaces and channels as a potential superior replacement for standard grayscale images, as well as the relative performance of open-source detectors and descriptors for general feature-based image registration purposes, based on a large [...] Read more.
The current study aimed to quantify the value of color spaces and channels as a potential superior replacement for standard grayscale images, as well as the relative performance of open-source detectors and descriptors for general feature-based image registration purposes, based on a large benchmark dataset. The public dataset UDIS-D, with 1106 diverse image pairs, was selected. In total, 21 color spaces or channels including RGB, XYZ, Y′CrCb, HLS, L*a*b* and their corresponding channels in addition to grayscale, nine feature detectors including AKAZE, BRISK, CSE, FAST, HL, KAZE, ORB, SIFT, and TBMR, and 11 feature descriptors including AKAZE, BB, BRIEF, BRISK, DAISY, FREAK, KAZE, LATCH, ORB, SIFT, and VGG were evaluated according to reprojection error (RE), root mean square error (RMSE), structural similarity index measure (SSIM), registration failure rate, and feature number, based on 1,950,984 image registrations. No meaningful benefits from color space or channel were observed, although XYZ, RGB color space and L* color channel were able to outperform grayscale by a very minor margin. Per the dataset, the best-performing color space or channel, detector, and descriptor were XYZ/RGB, SIFT/FAST, and AKAZE. The most robust color space or channel, detector, and descriptor were L*a*b*, TBMR, and VGG. The color channel, detector, and descriptor with the most initial detector features and final homography features were Z/L*, FAST, and KAZE. In terms of the best overall unfailing combinations, XYZ/RGB+SIFT/FAST+VGG/SIFT seemed to provide the highest image registration quality, while Z+FAST+VGG provided the most image features. Full article
(This article belongs to the Special Issue Image Processing and Computer Vision: Algorithms and Applications)
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23 pages, 1611 KiB  
Article
The Impact of Income Disparity on Food Consumption—Microdata from Rural China
by Jing Li, Kelin Chen, Chao Yan and Zhong Tang
Agriculture 2024, 14(5), 689; https://doi.org/10.3390/agriculture14050689 (registering DOI) - 28 Apr 2024
Abstract
This paper examines the relationship between income inequality and consumption, utilizing panel data from rural China over a span of four years to validate the application of relative income theory in the domain of food consumption. Food consumption represents a significant portion of [...] Read more.
This paper examines the relationship between income inequality and consumption, utilizing panel data from rural China over a span of four years to validate the application of relative income theory in the domain of food consumption. Food consumption represents a significant portion of expenditures for the low-income demographic and is of vital importance to China’s food security and agricultural development. To ascertain the impact of income inequality on food consumption, this paper employs a bi-directional fixed-effects model, a mediation effect model, and machine learning causal analysis methods. Utilizing four years of rural resident survey data from the China Health and Nutrition Survey database, the study empirically tests the effect of income inequality on various types of food consumption, the channels through which it operates, and the heterogeneity among different income groups and educational backgrounds. The findings indicate that (1) income inequality within rural communities positively influences food consumption, and this conclusion remains robust under endogeneity treatment and robustness checks, positively affecting the transformation of food consumption and healthy intake; (2) income inequality among rural residents promotes food consumption through two mediating channels: the “demonstration effect” and the “ratchet effect;” (3) the impact of income inequality on food consumption exhibits heterogeneity among rural residents of different income levels and educational backgrounds. Full article
(This article belongs to the Section Agricultural Economics, Policies and Rural Management)
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24 pages, 7759 KiB  
Article
Experimental and Numerical Prediction of Slamming Impact Loads Considering Fluid–Structure Interactions
by Tao Lu, Jiaxia Wang, Kun Liu and Xiaochao Zhao
J. Mar. Sci. Eng. 2024, 12(5), 733; https://doi.org/10.3390/jmse12050733 (registering DOI) - 28 Apr 2024
Abstract
Slamming impacts on water are common occurrences, and the whipping induced by slamming can significantly increase the structural load. This paper carries out an experimental study of the water entry of rigid wedges with various deadrise angles. The drop height and deadrise angle [...] Read more.
Slamming impacts on water are common occurrences, and the whipping induced by slamming can significantly increase the structural load. This paper carries out an experimental study of the water entry of rigid wedges with various deadrise angles. The drop height and deadrise angle are parametrically varied to investigate the effect of the entry velocity and wedge shape on the impact dynamics. A two-way coupled approach combing CFD method software STAR-CCM+12.02.011-R8 and the FEM method software Abaqus 6.14 is presented to analyze the effect of structural flexibility on the slamming phenomenon for a wedge and a ship model. The numerical method is validated through the comparison between the numerical simulation and experimental data. The slamming pressure, free surface elevation, and dynamic structural response, including stress and strain, in particular, are presented and discussed. The results show that the smaller the inclined angle at the bottom of the wedge-shaped body, the faster the entry speed into the water, resulting in greater impact pressure and greater structural deformation. Meanwhile, studies have shown that the bottom of the bow is an area of concern for wave impact problems, providing a basis for the assessment of ship safety design. Full article
(This article belongs to the Special Issue Advanced Analysis of Marine Structures—Edition II)
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15 pages, 3879 KiB  
Article
Structure of Polysaccharide from Dendrobium nobile Lindl. and its Mode of Action on TLR4 to Exert Immunomodulatory Effects
by Lian Li, Hang Chen, Guichun Huang, Yiyi Lv, Li Yao, Zhongxia Guo, Shuyi Qiu, Xiaodan Wang and Chaoyang Wei
Foods 2024, 13(9), 1356; https://doi.org/10.3390/foods13091356 (registering DOI) - 28 Apr 2024
Abstract
Dendrobium nobile Lindl. polysaccharide (DNP1) showed good anti-inflammatory activity in our previous study. In this study, the structural characterization of DNP1 and its mode of action on TLR4 were investigated. Structural characterization suggested that DNP1 was a linear glucomannan composed of (1 → [...] Read more.
Dendrobium nobile Lindl. polysaccharide (DNP1) showed good anti-inflammatory activity in our previous study. In this study, the structural characterization of DNP1 and its mode of action on TLR4 were investigated. Structural characterization suggested that DNP1 was a linear glucomannan composed of (1 → 4)-β-Manp and (1 → 4)-β-Glcp residues, and the acetyl group was linked to the C-2 of Manp. The possible repeating structural units of DNP1 were [→4)-2-OAc-β-Manp-(1→]3 →4)-β-Glcp-(1→. Surface plasmon resonance (SPR) binding test results showed that DNP1 did not bind directly to TLR4. The TLR4 and MD2 receptor blocking tests confirmed that DNP1 needs MD2 and TLR4 to participate in its anti-inflammatory effect. The binding energy of DNP1 to TLR4-MD2 was −7.9 kcal/mol, indicating that DNP1 could bind to the TLR4-MD2 complex stably. Therefore, it is concluded that DNP1 may play an immunomodulatory role by binding to the TLR4-MD2 complex and inhibiting the TLR4-MD2-mediated signaling pathway. Full article
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16 pages, 5480 KiB  
Article
Alginate Oligosaccharides Alleviate Salt Stress in Rice Seedlings by Regulating Cell Wall Metabolism to Maintain Cell Wall Structure and Improve Lodging Resistance
by Youwei Du, Huimin Zhao, Naijie Feng, Dianfeng Zheng, Aaqil Khan, Hang Zhou, Peng Deng, Yaxing Wang, Xutong Lu and Wenxin Jiang
Plants 2024, 13(9), 1215; https://doi.org/10.3390/plants13091215 (registering DOI) - 28 Apr 2024
Abstract
Salt stress is one of the major abiotic stresses that damage the structure and composition of cell walls. Alginate oligosaccharides (AOS) have been advocated to significantly improve plant stress tolerance. The metabolic mechanism by which AOS induces salt tolerance in rice cell walls [...] Read more.
Salt stress is one of the major abiotic stresses that damage the structure and composition of cell walls. Alginate oligosaccharides (AOS) have been advocated to significantly improve plant stress tolerance. The metabolic mechanism by which AOS induces salt tolerance in rice cell walls remains unclear. Here, we report the impact of AOS foliar application on the cell wall composition of rice seedlings using the salt-tolerant rice variety FL478 and the salt-sensitive variety IR29. Data revealed that salt stress decreased biomass, stem basal width, stem breaking strength, and lodging resistance; however, it increased cell wall thickness. In leaves, exogenous AOS up-regulated the expression level of OSCESA8, increased abscisic acid (ABA) and brassinosteroids (BR) content, and increased β-galacturonic activity, polygalacturonase activity, xylanase activity, laccase activity, biomass, and cellulose content. Moreover, AOS down-regulated the expression levels of OSMYB46 and OSIRX10 and decreased cell wall hemicellulose, pectin, and lignin content to maintain cell wall stability under salt stress. In stems, AOS increased phenylalamine ammonia-lyase and tyrosine ammonia-lyase activities, while decreasing cellulase, laccase, and β-glucanase activities. Furthermore, AOS improved the biomass and stem basal width and also enhanced the cellulose, pectin, and lignin content of the stem, As a result, increased resistance to stem breakage strength and alleviated salt stress-induced damage, thus enhancing the lodging resistance. Under salt stress, AOS regulates phytohormones and modifies cellulose, hemicellulose, lignin, and pectin metabolism to maintain cell wall structure and improve stem resistance to lodging. This study aims to alleviate salt stress damage to rice cell walls, enhance resistance to lodging, and improve salt tolerance in rice by exogenous application of AOS. Full article
(This article belongs to the Section Plant Response to Abiotic Stress and Climate Change)
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15 pages, 4425 KiB  
Article
Specificity of DNA ADP-Ribosylation Reversal by NADARs
by Bara Cihlova, Yang Lu, Andreja Mikoč, Marion Schuller and Ivan Ahel
Toxins 2024, 16(5), 208; https://doi.org/10.3390/toxins16050208 (registering DOI) - 28 Apr 2024
Abstract
Recent discoveries establish DNA and RNA as bona fide substrates for ADP-ribosylation. NADAR (“NAD- and ADP-ribose”-associated) enzymes reverse guanine ADP-ribosylation and serve as antitoxins in the DarT-NADAR operon. Although NADARs are widespread across prokaryotes, eukaryotes, and viruses, their specificity and broader physiological roles [...] Read more.
Recent discoveries establish DNA and RNA as bona fide substrates for ADP-ribosylation. NADAR (“NAD- and ADP-ribose”-associated) enzymes reverse guanine ADP-ribosylation and serve as antitoxins in the DarT-NADAR operon. Although NADARs are widespread across prokaryotes, eukaryotes, and viruses, their specificity and broader physiological roles remain poorly understood. Using phylogenetic and biochemical analyses, we further explore de-ADP-ribosylation activity and antitoxin functions of NADAR domains. We demonstrate that different subfamilies of NADAR proteins from representative E. coli strains and an E. coli-infecting phage retain biochemical activity while displaying specificity in providing protection from toxic guanine ADP-ribosylation in cells. Furthermore, we identify a myxobacterial enzyme within the YbiA subfamily that functions as an antitoxin for its associated DarT-unrelated ART toxin, which we termed YarT, thus presenting a hitherto uncharacterised ART-YbiA toxin–antitoxin pair. Our studies contribute to the burgeoning field of DNA ADP-ribosylation, supporting its physiological relevance within and beyond bacterial toxin–antitoxin systems. Notably, the specificity and confinement of NADARs to non-mammals infer their potential as highly specific targets for antimicrobial drugs with minimal off-target effects. Full article
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15 pages, 5361 KiB  
Article
Electromagnetic Performance Analysis of Dual-Three-Phase Dual-Rotor Flux-Switching Permanent Magnet Machines
by Yizhi Chen, Guishu Zhao, Zhengliang Li, Zhe Chang, Shuye Ding and Yuheng Zhou
Energies 2024, 17(9), 2102; https://doi.org/10.3390/en17092102 (registering DOI) - 28 Apr 2024
Abstract
In this paper, a novel dual-three-phase dual-rotor flux-switching permanent magnet (PM) (DRFSPM) machine, building upon conventional FSPM machines, is proposed, where the stator is equipped with dual PMs and dual armature windings, enabling it to operate in various working modes and provide fault [...] Read more.
In this paper, a novel dual-three-phase dual-rotor flux-switching permanent magnet (PM) (DRFSPM) machine, building upon conventional FSPM machines, is proposed, where the stator is equipped with dual PMs and dual armature windings, enabling it to operate in various working modes and provide fault tolerance in the event of PM or armature winding faults. Depending on the magnetization directions of the PMs, the proposed DRFSPM machine’s structure can be categorized as 6N-DRFSPM or NS-DRFSPM. In order to assess the electromagnetic performance of the proposed DRFSPM machines with two different magnetizing modes, the topology and operating principle of the two DRFSPM machines are introduced first. Then, the no-load air-gap flux density of the two proposed machines is investigated for a more optimized and purposeful design. Finally, a comparison of the electromagnetic performance between the two proposed DRFSPM machines is conducted by finite-element analysis (FEA), and the FEA-predicted results indicate that the proposed 6N-DRFSPM machine outperforms the NS-DRFSPM machine, as it exhibits a larger back-EMF and average torque and a smaller cogging torque and torque ripple. Full article
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15 pages, 992 KiB  
Article
Exploring the Association between Elevated Anxiety Symptoms and Low Skeletal Muscle Mass among Asymptomatic Adults: A Population-Based Study in Republic of Korea
by Eunsoo Kim, Sra Jung, Mi Yeon Lee, Chul-Hyun Park and Sung Joon Cho
Brain Sci. 2024, 14(5), 438; https://doi.org/10.3390/brainsci14050438 (registering DOI) - 28 Apr 2024
Abstract
Individuals with mental health problems are at higher risk of musculoskeletal diseases. However, the association between low muscle mass (LMM) and anxiety symptoms remains uninvestigated. This cross-sectional study enrolled 174,262 adults (73,833 women, 100,429 men), aged 18 to 89, who completed the anxiety [...] Read more.
Individuals with mental health problems are at higher risk of musculoskeletal diseases. However, the association between low muscle mass (LMM) and anxiety symptoms remains uninvestigated. This cross-sectional study enrolled 174,262 adults (73,833 women, 100,429 men), aged 18 to 89, who completed the anxiety scale and body composition analyses. Using bio-electrical impedance analysis, skeletal muscle mass index (SMI) was calculated based on appendicular skeletal muscle mass (ASM) (kg)/height (m2). LMM was defined as SMI < 7.0 kg/m2 in men and <5.4 kg/m2 in women. Anxiety symptoms were screened using the Clinical Useful Anxiety Outcome Scale (CUXOS) with cut-off scores of 20, 30, and 40. Multivariable logistic regression analyses were performed. LMM prevalence was 20.17% in women, 3.86% in men (p < 0.001). The prevalence of anxiety symptoms in LMM group decreased from mild (CUXOS > 20: women, 32.74%, men, 21.17%) to moderate (CUXOS > 30: 13.34%, 7.32%), to severe anxiety symptoms (CUXOS > 40: 4.00%, 1.73%). In multivariable-adjusted models, LMM was associated with mild (aOR (95% confidence interval)), women, 1.13 (1.08–1.17); men, 1.17 (1.08–1.27)), moderate (1.17 (1.11–1.24); 1.35 (1.19–1.53) and severe anxiety symptoms (1.18 (1.07–1.3), 1.36 (1.06–1.74)), demonstrating an increased risk of ORs with escalating anxiety severity. LMM was independently associated with a higher prevalence of anxiety symptoms. Full article
(This article belongs to the Special Issue Clinical Research on Mood Disorders: Opportunities and Challenges)
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10 pages, 260 KiB  
Article
Do Decision-Making Styles Predict Vagal Control? The Role of Resting Heart Rate Variability
by Adrián Alacreu-Crespo, Raquel Costa, Francisco Molins, Diana Abad-Tortosa, Noemí SanMiguel, Philippe Courtet and Miguel Ángel Serrano
Behav. Sci. 2024, 14(5), 369; https://doi.org/10.3390/bs14050369 (registering DOI) - 28 Apr 2024
Abstract
Decision-making styles are a habit-based propensity that drive behavior and affect daily life. Rational and intuitive decision-making styles have been associated with good mental health. However, the underlying mechanisms are not clear. In the last decade, high basal levels of heart rate variability [...] Read more.
Decision-making styles are a habit-based propensity that drive behavior and affect daily life. Rational and intuitive decision-making styles have been associated with good mental health. However, the underlying mechanisms are not clear. In the last decade, high basal levels of heart rate variability (HRV) have been proposed as an index of health and emotional control, and this could be one of the variables involved in the effects of decision making on health. Therefore, the aim of this study is to analyze the capability of decision-making styles to predict resting HRV. A cross-sectional study was conducted in a sample of 199 (119 women) young university students, and a resting ECG was recorded to extract frequency domain HRV variables. Subsequently, participants completed sociodemographic data and the General Decision-Making Style questionnaire (GDMS). Results showed that the intuitive style predicted high-frequency HRV, while the avoidant style predicted less low-frequency HRV. This study presents new data on the relationship between decision-making style and HRV, suggesting that the intuitive style has a cardioprotective effect, while the avoidant style is related to lower HRV, which has been associated with health vulnerability. In conclusion, this study contributes to the understanding of HRV and its potential as a biomarker for cognitive styles that may improve health. Full article
(This article belongs to the Section Health Psychology)
11 pages, 642 KiB  
Article
Toward Improved Detection of Cholesteatoma Recidivism: Exploring the Role of Non-EPI-DWI MRI
by Natalia Díaz Zufiaurre, Marta Calvo-Imirizaldu, Joan Lorente-Piera, Pablo Domínguez-Echávarri, Pau Fontova Porta, Manuel Manrique and Raquel Manrique-Huarte
J. Clin. Med. 2024, 13(9), 2587; https://doi.org/10.3390/jcm13092587 (registering DOI) - 28 Apr 2024
Abstract
Background: Cholesteatoma is a lesion capable of destroying surrounding tissues, which may result in significant complications. Surgical resection is the only effective treatment; however, the presence of cholesteatoma recidivism is common. This study evaluated the effectiveness of the Attic Exposure-Antrum Exclusion (AE-AE) surgical [...] Read more.
Background: Cholesteatoma is a lesion capable of destroying surrounding tissues, which may result in significant complications. Surgical resection is the only effective treatment; however, the presence of cholesteatoma recidivism is common. This study evaluated the effectiveness of the Attic Exposure-Antrum Exclusion (AE-AE) surgical technique in treating cholesteatomas and identifying factors associated with recidivism. Additionally, the study aimed to assess the utility of non-echo-planar diffusion MRI (non-EPI-DWI MRI) in detecting cholesteatoma recidivism in patients undergoing AE-AE surgery. Methods: The study involved 63 patients who underwent AE-AE surgery for primary acquired cholesteatoma and were followed up clinically and radiologically for at least five years. The radiological follow-up included a non-EPI-DWI MRI. Results: Results showed that the AE-AE technique successfully treated cholesteatomas, with a recidivism rate of 5.2%. The study also found that non-EPI-DWI MRI was a useful diagnostic tool for detecting cholesteatoma recidivism, although false positives could occur due to the technique’s high sensitivity. As Preoperative Pure-tone average (PTA) increases, there is a higher probability of cholesteatoma recidivism in imaging tests (p = 0.003). Conclusions: Overall, the study highlights the importance of the AE-AE surgical technique and non-EPI-DWI MRI in managing cholesteatoma recidivism in patients, providing valuable insights into associated risk factors and how to manage recidivism. Non-EPI-DWI MRI can assist in patient selection for revision surgery, reducing unnecessary interventions and associated risks while improving treatment outcomes and patient care. Full article
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8 pages, 469 KiB  
Article
The Efficacy of Platelet-Rich Plasma Injection Therapy in Obese versus Non-Obese Patients with Knee Osteoarthritis: A Comparative Study
by Juho Aleksi Annaniemi, Jüri Pere and Salvatore Giordano
J. Clin. Med. 2024, 13(9), 2590; https://doi.org/10.3390/jcm13092590 (registering DOI) - 28 Apr 2024
Abstract
Background/Objectives: Obesity is a common comorbidity in knee osteoarthritis (KOA) patients. Platelet-Rich Plasma (PRP) injection therapy may mitigate KOA. To further clarify potential patient selection for PRP injection therapy, we compared the outcomes in patients with different body mass index (BMI). Methods [...] Read more.
Background/Objectives: Obesity is a common comorbidity in knee osteoarthritis (KOA) patients. Platelet-Rich Plasma (PRP) injection therapy may mitigate KOA. To further clarify potential patient selection for PRP injection therapy, we compared the outcomes in patients with different body mass index (BMI). Methods: A total of 91 patients with mild to moderate KOA were treated with three intra-articular PRP injections at 10 to 14-day intervals. Range of motion (ROM), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and Visual Analogue Scale (VAS) were documented before and after the injections at 15 days, 6 months, 12 months, and at the last follow-up. Outcomes were compared between patients with a BMI over 30 kg/m2 (obese, n = 34) and under 30 kg/m2 (non-obese, n = 57). Results: Significant difference during the follow-up was detected in WOMAC score at the last follow-up favouring BMI under 30 group [17.8 ± 18.8 versus 10.5 ± 11.7, p = 0.023]. The odds ratio (OR) in BMI over 30 kg/m2 group for total knee arthroplasty was 3.5 (95% CI 0.3–40.1, p = 0.553), and OR for any arthroplasty was 7.5 (95% CI 0.8–69.8, p = 0.085) compared to non-obese patients. Conclusions: Obese patients benefitted from PRP injections in KOA but there is a minimal difference favouring non-obese patients in symptom alleviation in follow-up stages after 12 months. The risk of arthroplasty is higher for obese KOA patients. Full article
(This article belongs to the Section Orthopedics)
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13 pages, 690 KiB  
Article
Preliminary Envelope for Large Transport Aircrafts Operating with Non-Primary Fuels AVGAS, MOGAS and F76-Dieso
by José Luis Díaz Palencia
Eng 2024, 5(2), 720-732; https://doi.org/10.3390/eng5020039 (registering DOI) - 28 Apr 2024
Abstract
This study explores the operational implications and safety considerations of using non-primary fuels—AVGAS, MOGAS, and F76 Dieso—in military transport aircraft, against the backdrop of standard aviation fuels. Through an analysis of fuel properties such as vapor pressure, density, viscosity, freeze temperature, water solubility, [...] Read more.
This study explores the operational implications and safety considerations of using non-primary fuels—AVGAS, MOGAS, and F76 Dieso—in military transport aircraft, against the backdrop of standard aviation fuels. Through an analysis of fuel properties such as vapor pressure, density, viscosity, freeze temperature, water solubility, and thermal conductivity, this work outlines the operational envelopes for the mentioned non-primary fuels, highlighting the temperature and altitude limitations inherent to their use. The evaluation underscores the necessity of relevant testing, certification, and adherence to operational guidelines and constrains to ensure aircraft safety and reliability when standard fuels are unavailable, and hence, non-primary fuels may be required in special missions under emergency. Key findings include the specific altitude and temperature limitations for AVGAS and MOGAS to prevent fuel freezing and boiling, as well as the operational challenges posed by F76 Dieso due to its higher density and viscosity. The study also addresses the importance of managing water content in the fuel system, the flammability range of the non-primary fuels, and the considerations for fuel mixing to maintain aircraft performance and safety standards. This analysis aims to enhance the understanding of non-primary fuel usage in military transport aircraft, providing insights for system design, performance assessment, and the development of operational procedures to support military aviation in diverse operational scenarios. Full article
(This article belongs to the Section Chemical, Civil and Environmental Engineering)
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12 pages, 9613 KiB  
Article
Mn2Ga2S5 and Mn2Al2Se5 van der Waals Chalcogenides: A Source of Atomically Thin Nanomaterials
by Ivan V. Chernoukhov, Alexey V. Bogach, Kirill A. Cherednichenko, Ruslan A. Gashigullin, Andrei V. Shevelkov and Valeriy Yu. Verchenko
Molecules 2024, 29(9), 2026; https://doi.org/10.3390/molecules29092026 (registering DOI) - 28 Apr 2024
Abstract
Layered chalcogenides containing 3d transition metals are promising for the development of two-dimensional nanomaterials with interesting magnetic properties. Both mechanical and solution-based exfoliation of atomically thin layers is possible due to the low-energy van der Waals bonds. In this paper, we present the [...] Read more.
Layered chalcogenides containing 3d transition metals are promising for the development of two-dimensional nanomaterials with interesting magnetic properties. Both mechanical and solution-based exfoliation of atomically thin layers is possible due to the low-energy van der Waals bonds. In this paper, we present the synthesis and crystal structures of the Mn2Ga2S5 and Mn2Al2Se5 layered chalcogenides. For Mn2Ga2S5, we report magnetic properties, as well as the exfoliation of nanofilms and nanoscrolls. The synthesis of both polycrystalline phases and single crystals is described, and their chemical stability in air is studied. Crystal structures are probed via powder X-ray diffraction and high-resolution transmission electron microscopy. The new compound Mn2Al2Se5 is isomorphous with Mn2Ga2S5 crystallizing in the Mg2Al2Se5 structure type. The crystal structure is built by the ABCBCA sequence of hexagonal close-packing layers of chalcogen atoms, where Mn2+ and Al3+/Ga3+ species preferentially occupy octahedral and tetrahedral voids, respectively. Mn2Ga2S5 exhibits an antiferromagnetic-like transition at 13 K accompanied by the ferromagnetic hysteresis of magnetization. Significant frustration of the magnetic system may yield spin-glass behavior at low temperatures. The exfoliation of Mn2Ga2S5 layers was performed in a non-polar solvent. Nanolayers and nanoscrolls were observed using high-resolution transmission electron microscopy. Fragments of micron-sized crystallites with a thickness of 70–100 nanometers were deposited on a glass surface, as evidenced by atomic force microscopy. Full article
(This article belongs to the Section Materials Chemistry)
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15 pages, 3509 KiB  
Article
Burst Diagnosis Multi-Stage Model for Water Distribution Networks Based on Deep Learning Algorithms
by Sen Peng, Yuxin Wang, Xu Fang and Qing Wu
Water 2024, 16(9), 1258; https://doi.org/10.3390/w16091258 (registering DOI) - 28 Apr 2024
Abstract
Pipe bursts in water distribution networks (WDNs) pose significant threats to the safety of distribution networks, driving attention to deep learning-based burst detection and localization. However, the applicability of different pressure features still needs to be compared and verified. A large number of [...] Read more.
Pipe bursts in water distribution networks (WDNs) pose significant threats to the safety of distribution networks, driving attention to deep learning-based burst detection and localization. However, the applicability of different pressure features still needs to be compared and verified. A large number of nodes challenges deep learning with the excessive number of classification categories and low recognition accuracy. To address these problems, this paper extracts different burst pressure features, including pressure value, pressure difference, and pressure fluctuation ratio, and inputs one of these features into a Burst Diagnosis Multi-Stage Model (BDMM) based on three CS-LSTMs (a combination of the Cuckoo Search algorithm and a long short-term memory network). The first model addresses a binary classification problem, outputting labels indicating whether a pipe burst has occurred. The second one solves a multi-classification problem, outputting the label of the burst partition, and the third model also solves a multi-classification problem, outputting the ID of the bursting junction. The model is tested on a real network and outperforms ELM. For basic burst identification tasks using CS-LSTM, differences among the three features are minimal, while pressure difference and pressure fluctuation ratio exhibit superior performance to pressure value when resolving more complex problems like burst junction localization. Full article
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13 pages, 282 KiB  
Article
Lead Levels in the Most Consumed Mexican Foods: First Monitoring Effort
by Alejandra Cantoral, Larissa Betanzos-Robledo, Sonia Collado-López, Betzabeth A. García-Martínez, Héctor Lamadrid-Figueroa, Rosa M. Mariscal-Moreno, Araceli Díaz-Ruiz, Camilo Ríos and Martha María Téllez-Rojo
Toxics 2024, 12(5), 318; https://doi.org/10.3390/toxics12050318 (registering DOI) - 28 Apr 2024
Abstract
Globally, there is growing concern over the presence of lead (Pb) in foods because it is a heavy metal with several toxic effects on human health. However, monitoring studies have not been conducted in Mexico. In this study, we estimated the concentrations of [...] Read more.
Globally, there is growing concern over the presence of lead (Pb) in foods because it is a heavy metal with several toxic effects on human health. However, monitoring studies have not been conducted in Mexico. In this study, we estimated the concentrations of Pb in the most consumed foods and identified those that exceeded the maximum limits (MLs) for Pb in foods established by the International Standards. Based on the Mexican National Health and Nutrition Survey, 103 foods and beverages were selected and purchased in Mexico City retail stores and markets. Samples were analyzed twice using atomic absorption spectrophotometry. Values above the limit of quantification (0.0025 mg/kg) were considered to be detected. The percentage of detected values was 18%. The highest concentration was found in infant rice cereal (1.005 mg/kg), whole wheat bread (0.447 mg/kg), pre-cooked rice (0.276 mg/kg), black pepper (0.239 mg/kg), and turmeric (0.176 mg/kg). Among the foods with detected Pb, the levels in infant rice cereal, whole wheat bread, pre-cooked rice, and soy infant formula exceeded the MLs. The food groups with the highest percentages of exceeded MLs were baby foods (18%) and cereals (11%). Monitoring the concentration of contaminants in foods is essential for implementing food safety policies and protecting consumer health. Full article
(This article belongs to the Section Metals and Radioactive Substances)
8 pages, 1546 KiB  
Article
External Radiation Assistance of Neutrinoless Double Electron Capture
by Vladimir N. Kondratyev and Feodor F. Karpeshin
Atoms 2024, 12(5), 27; https://doi.org/10.3390/atoms12050027 (registering DOI) - 28 Apr 2024
Abstract
The influence of electromagnetic radiation on nuclear processes is applied to an example of a neutrinoless double electron capture (0ν2ec). For cases with X-ray free-electron lasers (X-ray FELs) and/or inverse Compton X-ray sources, it was shown that such a decay can [...] Read more.
The influence of electromagnetic radiation on nuclear processes is applied to an example of a neutrinoless double electron capture (0ν2ec). For cases with X-ray free-electron lasers (X-ray FELs) and/or inverse Compton X-ray sources, it was shown that such a decay can be significantly enhanced by tuning the system to the resonant conditions through the absorption and/or emission of a photon with the decay resonance defect energy Δ. In this case, the 0v2ec decay rate Γ2e of nuclide Z grew linearly with field intensity (S/Sz) up to the X-ray flux power Sm~Z6, while Sz~Z6 (Γ/Δ)2 with decay width Γ of a daughter atom. For the case of 78Kr → 78Se − 0ν2eL1L1 capture we find Sz~109 W cm−2 and Sm~1017 W cm−2 which indicate a possibility of increasing decay rate to eight orders of magnitude or even larger. Full article
(This article belongs to the Section Nuclear Theory and Experiments)
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13 pages, 2356 KiB  
Article
DeepSub: Utilizing Deep Learning for Predicting the Number of Subunits in Homo-Oligomeric Protein Complexes
by Rui Deng, Ke Wu, Jiawei Lin, Dehang Wang, Yuanyuan Huang, Yang Li, Zhenkun Shi, Zihan Zhang, Zhiwen Wang, Zhitao Mao, Xiaoping Liao and Hongwu Ma
Int. J. Mol. Sci. 2024, 25(9), 4803; https://doi.org/10.3390/ijms25094803 (registering DOI) - 28 Apr 2024
Abstract
The molecular weight (MW) of an enzyme is a critical parameter in enzyme-constrained models (ecModels). It is determined by two factors: the presence of subunits and the abundance of each subunit. Although the number of subunits (NS) can potentially be obtained from UniProt, [...] Read more.
The molecular weight (MW) of an enzyme is a critical parameter in enzyme-constrained models (ecModels). It is determined by two factors: the presence of subunits and the abundance of each subunit. Although the number of subunits (NS) can potentially be obtained from UniProt, this information is not readily available for most proteins. In this study, we addressed this gap by extracting and curating subunit information from the UniProt database to establish a robust benchmark dataset. Subsequently, we propose a novel model named DeepSub, which leverages the protein language model and Bi-directional Gated Recurrent Unit (GRU), to predict NS in homo-oligomers solely based on protein sequences. DeepSub demonstrates remarkable accuracy, achieving an accuracy rate as high as 0.967, surpassing the performance of QUEEN. To validate the effectiveness of DeepSub, we performed predictions for protein homo-oligomers that have been reported in the literature but are not documented in the UniProt database. Examples include homoserine dehydrogenase from Corynebacterium glutamicum, Matrilin-4 from Mus musculus and Homo sapiens, and the Multimerins protein family from M. musculus and H. sapiens. The predicted results align closely with the reported findings in the literature, underscoring the reliability and utility of DeepSub. Full article
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11 pages, 4882 KiB  
Article
Modulation of Diamond PN Junction Diode with Double-Layered n-Type Diamond by Using TCAD Simulation
by Caoyuan Mu, Genzhuang Li, Xianyi Lv, Qiliang Wang, Hongdong Li, Liuan Li and Guangtian Zou
Electronics 2024, 13(9), 1703; https://doi.org/10.3390/electronics13091703 (registering DOI) - 28 Apr 2024
Abstract
This study proposed a novel double-layer junction termination structure for vertical diamond-based PN junction diodes (PND). The effects of the geometry and doping concentration of the junction termination structure on the PNDs’ electrical properties are investigated using Silvaco TCAD software (Version 5.0.10.R). It [...] Read more.
This study proposed a novel double-layer junction termination structure for vertical diamond-based PN junction diodes (PND). The effects of the geometry and doping concentration of the junction termination structure on the PNDs’ electrical properties are investigated using Silvaco TCAD software (Version 5.0.10.R). It demonstrates that the electric performances of PND with a single n-type diamond layer are sensitive to the doping concentration and electrode location of the n-type diamond. To further suppress the electric field crowding and obtain a better balance between breakdown voltage and on-resistance, a double-layer junction termination structure is introduced and evaluated, yielding significantly improved electronic performances. Those results provide some useful thoughts for the design of vertical diamond PND devices. Full article
(This article belongs to the Special Issue Recent Advances in Wide Bandgap Semiconductors)
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17 pages, 1125 KiB  
Article
Plant-Growth-Promoting Rhizobacteria and Arbuscular Mycorrhizal Fungi May Improve Soil Fertility and the Growth, Nutrient Uptake, and Physiological Performance of Batavia Lettuce (Lactuca sativa L. var. longifolia) Plants
by Theocharis Chatzistathis, Konstantinos Zoukidis, Christos Vasilikiotis, Antonios Apostolidis, Anastasia E. Giannakoula, Areti Bountla and Apostolos Chatziathanasiadis
Horticulturae 2024, 10(5), 449; https://doi.org/10.3390/horticulturae10050449 (registering DOI) - 28 Apr 2024
Abstract
A greenhouse experiment was realized to investigate the effects of plant-growth-promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF) on soil quality and the growth, nutrient uptake, and physiological performance of Batavia lettuce (Lactuca sativa L. var. longifolia). For this purpose, six [...] Read more.
A greenhouse experiment was realized to investigate the effects of plant-growth-promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF) on soil quality and the growth, nutrient uptake, and physiological performance of Batavia lettuce (Lactuca sativa L. var. longifolia). For this purpose, six fertilization treatments were applied: (i) inorganic fertilization (I.F.—control, i.e., usual/conventional fertilization), (ii) I.F. + PGPR, (iii) I.F. + PGPR + AMF, (iv) manure (MAN), (v) MAN + PGPR, and (vi) MAN + PGPR + AMF. Soil fertility was influenced by the treatments, and soil respiration (CO2 flux) was significantly increased after applications of PGPR and AMF. Following MAN fertilization in particular, an approximately sixfold higher soil respiration value was recorded following the MAN + PGPR + AMF treatment compared to the control (I.F.). Root, leaf, and total biomass dry weights were significantly affected by the PGPR and AMF applications, mainly following the I.F. treatment. In contrast, K, Mg, and Fe uptake was significantly influenced by PGPR and AMF application following the MAN treatment. The SPAD value, performance index (PI), photosynthetic rate, and stomatal conductance were significantly higher in the I.F. + PGPR + AMF-treated plants compared to the control plants. Overall, these data prove the beneficial roles of PGPR and AMF in soil quality and fertility and the nutrient uptake and physiological performance of lettuce plants. However, further clarification is needed in the near future to test the interaction effects between PGPR, AMF, and the type of fertilizer used (organic or inorganic). Full article
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10 pages, 1301 KiB  
Article
Test–Retest Reliability of a Motorized Resistance Device for Measuring Throwing Performance in Volleyball Athletes
by Eleftherios Paraskevopoulos, Anna Christakou, George Plakoutsis, George M. Pamboris and Maria Papandreou
Biomechanics 2024, 4(2), 259-268; https://doi.org/10.3390/biomechanics4020015 (registering DOI) - 28 Apr 2024
Abstract
Throwing performance is a critical aspect of sports, particularly in overhead activities, necessitating reliable assessment methods. This study explores the test–retest reliability of throwing performance metrics measured by the 1080 Sprint, a robotic device integrating linear position technology and an electric motor. Specifically [...] Read more.
Throwing performance is a critical aspect of sports, particularly in overhead activities, necessitating reliable assessment methods. This study explores the test–retest reliability of throwing performance metrics measured by the 1080 Sprint, a robotic device integrating linear position technology and an electric motor. Specifically focusing on professional volleyball athletes with scapular dyskinesis, the study draws data from a previously published investigation on the impact of mirror cross exercise. Thirty-nine athletes were recruited, aged 21.9 ± 3.6 years, height 1.79 ± 0.3 m weight 68.5 ± 19.8 kg, and body mass index 21.3 ± 3.2 kg/m2, meeting stringent inclusion criteria. One-sample t-tests indicated no statistically significant differences between test–retest trials. The study revealed excellent reliability of the 1080 Sprint, with intraclass correlation coefficient (ICC) values exceeding 0.99 for all metrics, including speed, force, and power. The standard error of measurement (SEM) calculation revealed that the Sprint 1080 motorized resistance device demonstrates high precision in measuring throwing performance. Bland and Altman plots indicated minimal systematic bias across all metrics, encompassing speed, force, and power. The provision of the minimum detectable change (MDC) for each variable of the Sprint 1080 motorized resistance device offers coaches a valuable tool to identify performance improvements in volleyball athletes. In conclusion, the present study shows that the 1080 Sprint is valid and reliable for measuring throwing performance in volleyball athletes for monitoring purposes. Full article
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12 pages, 1601 KiB  
Article
Using Temporally and Spatially Resolved Measurements to Improve the Sensitivity of Fluorescence-Based Immunoassays
by Ran Kremer, Shira Roth, Avital Bross, Amos Danielli and Yair Noam
Biosensors 2024, 14(5), 220; https://doi.org/10.3390/bios14050220 (registering DOI) - 28 Apr 2024
Abstract
Detecting low concentrations of biomarkers is essential in clinical laboratories. To improve analytical sensitivity, especially in identifying fluorescently labeled molecules, typical optical detection systems, consisting of a photodetector or camera, utilize time-resolved measurements. Taking a different approach, magnetic modulation biosensing (MMB) is a [...] Read more.
Detecting low concentrations of biomarkers is essential in clinical laboratories. To improve analytical sensitivity, especially in identifying fluorescently labeled molecules, typical optical detection systems, consisting of a photodetector or camera, utilize time-resolved measurements. Taking a different approach, magnetic modulation biosensing (MMB) is a novel technology that combines fluorescently labeled probes and magnetic particles to create a sandwich assay with the target molecules. By concentrating the target molecules and then using time-resolved measurements, MMB provides the rapid and highly sensitive detection of various biomarkers. Here, we propose a novel signal-processing algorithm that enhances the detection and estimation of target molecules at low concentrations. By incorporating both temporally and spatially resolved measurements using human interleukin-8 as a target molecule, we show that the new algorithm provides a 2–4-fold improvement in the limit of detection and an ~25% gain in quantitative resolution. Full article
(This article belongs to the Special Issue Nano-Biosensors for Detection and Monitoring (Volume II))
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14 pages, 3472 KiB  
Article
Endurant Stent Graft for Treatment of Abdominal Aortic Aneurysm Inside and Outside of the Instructions for Use for the Proximal Neck: A 14-Year, Single-Center Experience
by Giulio Accarino, Francesco De Vuono, Giancarlo Accarino, Giovanni Fornino, Aniello Enrico Puca, Rodolfo Fimiani, Valentina Parrella, Giovanni Savarese, Sergio Furgiuele, Carmine Vecchione, Gennaro Galasso and Umberto Marcello Bracale
J. Clin. Med. 2024, 13(9), 2589; https://doi.org/10.3390/jcm13092589 (registering DOI) - 28 Apr 2024
Abstract
Aim: To assess the medium and long-term performance of the Endurant stent graft in a cohort of consecutive patients treated with this device for an abdominal aortic aneurysm (AAA) both inside and outside of the instructions for use (IFU) and to find [...] Read more.
Aim: To assess the medium and long-term performance of the Endurant stent graft in a cohort of consecutive patients treated with this device for an abdominal aortic aneurysm (AAA) both inside and outside of the instructions for use (IFU) and to find factors influencing the outcomes. Methods: Our observational, retrospective, single-center study included all patients who consecutively underwent endovascular aneurysm repair with the Endurant stent graft from February 2009 to January 2023. Patients with an AAA to treat according to current guidelines were included. Patients were divided into two groups: Group 1 inside of the IFUs and Group 2 outside of the IFUs for the proximal aortic neck. Patients were followed up after the procedure with computed angiography tomography, ultrasound examination, and interviews. Aneurysm-related mortality, procedure-related reinterventions, and type IA and III endoleaks were considered primary endpoints. Secondary endpoints included aneurysmal sac variations and graft thrombosis. Results: A total of 795 patients were included, 650 in Group 1 and 145 in Group 2; 732 were males, and the mean age was 74 ± 8. Anamnestic baseline did not differ between the two groups. Neck length, width, and angulation were different between the two groups (all p < 0.001). A total of 40 patients had a ruptured AAA, while 56 were symptomatic. At a mean follow-up of 43 ± 39 months, aneurysm-related mortality was less than 1%, and 82 endoleak (10.5%) were observed. Overall endoleak rate and type 1A endoleak, as well as procedure-related reintervention, were significantly more frequent in Group 2. Sac regression of at least 5 mm was observed in 65.9% of cases. AAAs larger than 60.5 mm carried a higher risk of endoleak (HR: 1.025; 95% CI: 1.013–1.37; p < 0.001) and proximal necks shorter than 13.5 mm carried a higher type 1A risk (HR: 0.890; 95% CI: 0.836–0.948; p < 0.001). Patients without chronic obstructive pulmonary disease and taking lipid-lowering drugs had an overall more consistent sac-shrinking rate. Conclusions: The Endurant stent graft proves safe and reliable. Out-of-IFU treatment has poorer medium and long-term outcomes. Some conditions influence medium and long-term reintervention risk and sac behavior. Patients with bigger aneurysms, proximal necks shorter than 13.5 mm, and chronic obstructive pulmonary disease should be more carefully evaluated during follow-up. Consistent follow-up is in keeping low aneurysm-related mortality. Personalized risk profiles and peri and postoperative management strategies are needed. Full article
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13 pages, 2126 KiB  
Article
A Study on Endogenous Inhibitors of Nitraria roborowskii Kom. Seeds
by Shangfu Ren, Lamei Jiang and Guanghui Lv
Forests 2024, 15(5), 773; https://doi.org/10.3390/f15050773 (registering DOI) - 28 Apr 2024
Abstract
Nitraria roborowskii Kom. seeds have deep dormancy characteristics. Under natural conditions, the germination rate of the seeds is low, and the germination time is long. Therefore, exploring the reasons for seed dormancy is highly important. The results showed that the extracts of the [...] Read more.
Nitraria roborowskii Kom. seeds have deep dormancy characteristics. Under natural conditions, the germination rate of the seeds is low, and the germination time is long. Therefore, exploring the reasons for seed dormancy is highly important. The results showed that the extracts of the methanol phase, ethyl acetate phase, petroleum ether phase and water phase of N. roborowskii seeds all had a significant inhibitory effect on the germination rate and germination index of Brassica rapa seeds, among which the extract of the methanol phase had the strongest inhibitory effect, and the inhibitory effect decreased in the following order from the strongest to the weakest: methanol phase > ethyl acetate phase > petroleum ether phase > water phase. The components of the methanol phase, ethyl acetate phase and petroleum ether phase ether extracts of N. roborowskii seeds were identified by gas chromatography–mass spectrometry (GC–MS). The experimental results showed that the organic phase extracts of N. roborowskii seeds contained a variety of inhibitory compounds, which included 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-dibutyl phthalate; 4-((1E)-3-hydroxy-1-propenyl)-2-methoxyphenol; 13-docosenamide, (Z)-; 3-hydroxy-4-methoxybenzoic acid; vanillin; 2,4-di-tert-butylphenol; and cyclohexane, ethyl-. The seeds of N. roborowskii contain a variety of endogenous inhibitors, which are the main reason for its seed dormancy. Full article
(This article belongs to the Section Forest Ecophysiology and Biology)
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